Effect of Artificial Light Exposure on Estrus Synchronization and Hormone Secretion of Minxinan Black Female Rabbits
-
摘要:
目的 研究不同光源诱导闽西南黑母兔同期发情与激素之间的关系，以提高闽西南黑兔的繁殖性能。 方法 选用150日龄未经产雌性闽西南黑兔，于LED灯和节能灯下饲养1周，光照周期L：D=16：8 h，对照组只提供自然光照。于补光前1 d、光照3 d后和光照7 d后观察记录发情率并耳缘静脉采血。采用ELISA方法，研究不同光源对闽西南黑兔褪黑激素、促卵泡素和雌二醇分泌的影响。 结果 （1）LED灯和节能灯光组的发情率明显高于对照组，差异显著，表明LED灯和节能灯补光对闽西南黑母兔的发情率有明显影响；（2）ELISA试剂盒检测不同光源下血清中褪黑激素、促卵泡素和雌二醇素含量，LED灯和节能灯光处理使150日龄闽西南黑母兔在光照后3 d和光照后7 d血清中褪黑激素水平显著降低，而促卵泡素和雌二醇激素水平有增加的趋势，但差异不显著。 结论 不同光源长光照可以显著影响150日龄未经产雌性闽西南黑兔同期发情，且对血浆中的褪黑激素具有明显下降作用，但对促卵泡素和雌二醇影响较小。 Abstract:Objective Effects of exposure to artificial lights on estrus synchronization and hormone secretion in female rabbits were investigated in order to improve the reproductive performance of Minxinan black rabbit. Method Non-breeding Minxinan black female rabbits at the age of 150 d were raised under LED and energy-saving lamps or natural light for one week. A daily exposure cycle of L:D=16:8 h on the treatment animals or of the natural light conditions for the control group was applied. Estrus occurrence rates of the rabbits on both groups were recorded 1 d before as well as 3 d and 7 d after the beginning of the treatment. At the same time, blood samples were collected from the ear vein of the rabbits for serum melatonin, follicle stimulating hormone, and estradiol determinations by ELISA. Result (1) The artificial light exposure significantly increased estrus in the rabbits. (2) After 3 d and 7 d of the treatment, the serum melatonin in the rabbits decreased significantly, while the follicle stimulating hormone and estradiol increased slightly, whereas, no significant differences on those were detected in the control group. Conclusion The artificial light exposure appeared to significantly affect the estrus of 150-d-old non-parous Minxinan black female rabbits. It also exerted a significant effect on the serum melatonin, but not on the follicle stimulating hormone or estradiol in the rabbits. -
Key words:
- LED light /
- energy-saving light /
- estrus synchronization /
- hormone /
- Minxinan black rabbit
-
表 1 日粮组成
Table 1. Dietary composition
成分
Composition含量
Content/%粗蛋白Crude protein ≥17.0 粗纤维Crude fibre 12.0~23.0 粗灰分Curde ash ≤11.0 钙Calcium 0.5~1.5 总磷Total phosphorus ≥0.4 氯化钠Sodium chloride 0.3~1.0 赖氨酸Lysine ≥0.7 蛋氨酸+胱氨酸Methionine and csytine ≥0.7 水分Moisture ≤14.0 
下载: 导出CSV
表 2 LED灯和节能灯光在不同时间点对闽西南黑母兔发情率的影响
Table 2. Estrus rates of treatment rabbits at various sampling time
(单位/%) 项目
ItemsLED灯组
LED light group节能灯组
Energy saving light group对照组
Control group光照前1 d 1 day before the light 45.0±0.25 42.5±0.25 42.5±0.25 光照3 d后3 days after the light 52.5±0.25a 52.5±0.25a 12.5±0.25b 光照7 d后7 days after the light 95.0±0.25a 90.0±0.25b 52.5±0.25c 注：同行数据后不同小写字母表示差异显著(P < 0.05)。表 3~5同。
Note：Different lowercase letters mean significant difference (P < 0.05). The same as Table 3-5.
下载: 导出CSV
表 3 LED灯和节能灯光在不同时间点对闽西南黑母兔血清褪黑激素(MLT)水平的影响
Table 3. Serum melatonin of treatment rabbits at various sampling time
[单位/(ng·mL-1)] 项目
ItemsLED灯组
LED light group节能灯组
Energy saving light group对照组
Control group光照前1 d 1 day before the light 21.16±1.41 21.16±1.41 21.37±1.47 光照3 d后3 days after the light 14.96±1.26c 15.16±1.24b 20.17±1.56a 光照7 d后7 days after the light 11.27±1.25c 13.17±1.21b 19.17±1.61a
下载: 导出CSV
表 4 LED光和节能灯光组在不同时间点对闽西南黑母兔血清促卵泡素(FSH)水平的影响
Table 4. Serum follicle stimulating hormone of treatment rabbits at various sampling time
[单位/(mIU·mL-1)] 项目
ItemsLED灯组
LED light group节能灯组
Energy saving light group对照组
Control group光照前1 d 1 day before the light 7.35±0.41 7.21±0.41 7.19±0.43 光照3 d后3 days after the light 8.69±0.24 8.24±0.14 7.61±0.24 光照7 d后7 days after the light 9.24±0.21 8.73±0.17 7.71±0.19
下载: 导出CSV
表 5 LED光和节能灯光组在不同时间点对母兔血清雌二醇(E2)水平的影响
Table 5. Serum estradiol of treatment rabbits at various sampling time
[单位/(pg·mL-1)] 项目
ItemsLED灯组
LED light group节能灯组
Energy saving light group对照组
Control group光照前1 d 1 day before the light 120.13±1.41 120.6±1.41 121.37±1.74 光照3 d后3 day after the light 150.16±1.34 147.16±1.42 127.17±1.35 光照7 d后7 day after the light 210.17±1.31 198.17±1.24 161.00±1.47
下载: 导出CSV
-
[1] THEAU-CLÉMENT M.Preparation of the rabbit doe to insemination:a review[J]. World Rabbit Sci, 2007, 15:61-80. [2] THEAU-CLÉMENT M, LEBAS F, DRION P, et al. Evolution de laproduction d'anticorps anti-PMSG enfonction de la dose etdu nombred injections[J]. Relation avec la productivité deslapines, 7es Journées de la Recherche Cunicole, Lyon, 1998, 3(1):225-228. [3] VIRÁGGY, PAPP Z, RAFAI P, et al. Effect of an intermittent lighting schedule on doe and suckling rabbit's performance[J]. World Rabbit Sci, 2000, 8(1):477-481. [4] 张雅琼, 秦应和, 阎英凯, 等.不同剂量马绒毛膜促性腺激素对母兔同期发情效果的影响[J].中国畜牧杂志, 2010, 46(23):23-26. http://www.cnki.com.cn/Article/CJFDTOTAL-ZGXM201023008.htmZHANG Y Q, QIN Y H, YAN Y K, et al. Effect of different doses equine chorionic gonadotropin on the estrus of female rabbits[J]. Chinese Journal of Animal Science, 2010, 46(23):23-26.(in Chinese) http://www.cnki.com.cn/Article/CJFDTOTAL-ZGXM201023008.htm [5] 谭卫国, 李继兴, 谭小平, 等.光照与药物对母兔同期发情影响的研究[J].黑龙江畜牧兽医, 2014(6):122-123. http://www.cnki.com.cn/Article/CJFDTOTAL-HLJX201412064.htmTAN W G, LI J X, TAN X P, et al. Experimental study for the effects of lighting and drug on estrus synchronization in female rabbits[J]. Heilongjiang Animal Science and Veterinary Medicine, 2014(6):122-123.(in Chinese) http://www.cnki.com.cn/Article/CJFDTOTAL-HLJX201412064.htm [6] 谷子林, 张国磊, 马学会, 等.光照对家兔的影响及其控制[J].今日畜牧兽医, 2006(2):37. http://d.old.wanfangdata.com.cn/Periodical/hebxmsy200602030GU Z L, ZHANG G L, MA X H, et al. Effect of light on rabbits and its control[J]. Today Animal Husbandry and Veterinary Medicine, 2006(2):37.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/hebxmsy200602030 [7] 宋亚鹏. LED光源与节能灯对母兔同期发情影响[J].中国动物保健, 2014(12):70-71. doi: 10.3969/j.issn.1008-4754.2014.12.032SONG Y P. Effect of LED light source and energy saving lamp on estrus of female rabbits[J].China Animal Health, 2014(12):70-71.(in Chinese) doi: 10.3969/j.issn.1008-4754.2014.12.032 [8] 李士栋.光照刺激光源和季节对母兔繁殖性能的影响[J].中国养兔, 2016(4):18-20. doi: 10.3969/j.issn.1005-6327.2016.04.005LI S D, Effect of photostimulation light source and season on reproductive performance of rabbit does[J]. Chinese Journal of Rabbit Farming, 2016(4):18-20.(in Chinese) doi: 10.3969/j.issn.1005-6327.2016.04.005 [9] REBOLIAR P G, UBILLA E, ALVARIO JM R, et al. Influencia del nivel de receptividad sexual sobre el estradiol plasmático y la respuesta ovulatoria durante el post-parto de la coneja[J]. Rev Esp Fisiology, 1992, 48:13-18. [10] 曹静, 陈耀星, 王子旭, 等.单色光对肉鸡生长发育的影响[J].中国农业科学, 2007, 40(10):2350-2353. doi: 10.3321/j.issn:0578-1752.2007.10.032CAO J, CHEN Y X, WANG Z X, et al. Effect of monochromatic light on broiler growth[J]. Scientia Agriculture Sinica, 2007, 40(10):2350-2353.(in Chinese) doi: 10.3321/j.issn:0578-1752.2007.10.032 [11] 范国成, 王伟新, 林雄杰, 等.LED在我国农业相关领域的研究与应用[J].福建农业科技, 2013, (2):106-107. http://d.old.wanfangdata.com.cn/Periodical/fjnykj201301048FAN G C, WANG W X, LIN X J, et al. Research and application of light emitting diode in agricultural-related fields in China[J]. Fujian Agricultural Science and Technology, 2013, (2):106-107.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/fjnykj201301048 [12] 徐志刚.LED在现代农业中的应用[J].半导体技术, 2010, 35(9):175-180. http://d.old.wanfangdata.com.cn/Periodical/kcjyyj201719277XU Z G. LED applied in modern agriculture[J]. Semiconductor Technology, 2010, 35(9):175-180.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/kcjyyj201719277 [13] 陈岩峰, 陈冬金, 孙世坤, 等.闽西南黑兔繁殖、生长、屠宰性能和肉质特性的研究[J].畜牧与兽医, 2015, 47(2):64-67. http://d.old.wanfangdata.com.cn/Periodical/xmysy201502016CHEN Y F, CHEN D J, SUN S K, et al. Reproduction, growth, carcass characteristics and meat quality of local breeds rabbits[J]. Animal Husbandry & Veterinary Medicine, 2015, 47(2):64-67.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/xmysy201502016 [14] 桑雷, 王锦祥, 孙世坤, 等.闽西地方肉兔与新西兰兔屠宰和肉质性状研究[J].福建农业学报, 2018, 33(9):888-892. doi: 10.19303/j.issn.1008-0384.2018.09.002SANG L, WANG J X, SUN S K, et al. Carcass characteristics and meat quality of local breeds and New Zealand white rabbits[J]. Fujian Journal of Agricultural Sciences, 2018, 33(9):888-892.(in Chinese) doi: 10.19303/j.issn.1008-0384.2018.09.002 [15] 任永军, 雷岷, 邝良德, 等.不同光照强度对哺乳母兔同期发情及繁殖性能的影响[J].中国养兔, 2014(2):4-6, 12. doi: 10.3969/j.issn.1005-6327.2014.02.001REN Y J, LEI M, KUANG L D, et al. Effect of different light intensity on estrus synchronization and reproductive performance of lactation mother rabbit[J]. Chinese Journal of Rabbit Farming, 2014(2):4-6, 12.(in Chinese) doi: 10.3969/j.issn.1005-6327.2014.02.001 [16] 孟繁臣. LED光源不同光色与光照强度对伊拉肉兔母兔繁殖性能的影响[D].杨凌: 西北农林科技大学, 2017.MENG F C. Effects of different light color and light intensity of LED on reproductive performance of hyla female rabbits[D]. Yangling: Northwest A & F University, 2017.(in Chinese) [17] KALABA Z M, ABDEL-KHALEK A E, et al. Reproductive performance of rabbit does and productivity of their kits in response to colour of light[J]. Asian Journal of Animal and Veterinary Advances, 2011, (8):814-822.(in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=Open J-Gate000003868969 [18] MOUSA-BALABEL T, MOHAMED R. Effect of different photoperiods and melatonin treatment on rabbit reproductive performance[J]. The Veterinary Quarterly, 2011, 31(4):165-71.(in Chinese) doi: 10.1080/01652176.2011.642533 [19] BESENFELDER U, THEAU-CLÉMENT M, SABBIONI E, et al. Effects of different light intensities on quality of spermatozoa in rabbits[J]. World Rabbit Science, 2005, (12):227-234.(in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=8f04dd7119a3cd4e82aa7ede0af6c7a8 [20] ULENT G, MILTON H. Effects of photoperiod, pinealectomy and MT implant on testicular development in Juvenile Siberian Hanmsters (Phodopussungonus)[J]. Biology of Reproduction, 1994, 51:1181-1187. doi: 10.1095/biolreprod51.6.1181 -
