Preparation, Characterization and Antimicrobial Activity of Carbon Dots from Pilei of Dictyophora echinovolvata
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摘要:
目的 利用废旧生物质资源制备量子点并增加细菌病害防治的资源。 方法 以刺托竹荪菌盖采用水热法合成碳量子点，并用透射电镜扫描、红外光谱、XRD和荧光分析对碳量子点进行表征。选取青枯雷尔氏菌Ralstonia solanacearum和地毯草黄单胞菌Xanthomonas axonopodis作为供试菌种，采用抑菌圈法对碳量子点的抑菌性能进行研究。 结果 该方法制得的碳量子点近似球形，粒径均匀，分散性好。其发射波长依赖于激发波长，在紫外灯的照射下，出现明亮的蓝色荧光。碳量子点对青枯雷尔氏菌和地毯草黄单胞菌均有明显的抑菌作用。 结论 获得了一种可用于防治植物细菌病害的量子点。 Abstract:Objective Utilization of mushroom biomass waste to prepare carbon dots (CDs) for the prevention and control of diseases on plants was studied. Method CDs were obtained by hydrothermal oxidation of pilei of Dictyophora echinovolvata and characterized by the transmission electron microscopy (TEM), Fourier transform infrared spectrophoto-metry (FT-IR), x-ray diffraction (XRD), and fluorescene spectrophotometry. Antibacterial properties of the CDs were determined by measurements of diameter of the bacteriostatic circle on Ralstonia solanacearum and Xanthomonas axonopodis. Result The CDs obtained displayed an appropriate spherical morphology and uniform size with good dispersion. They emitted bright blue photoluminescence under UV excitation, exhibited an excitation-dependent PL behavior, and demonstrated excellent antibacterial activities against R. solanacearum and X. axonopodis. Conclusion The CDs with a potential of preventing and controlling plant diseases by bacterial infection were successful prepared in laboratory. -
Key words:
- Carbon dots /
- hydrothermal method /
- antibacterial activity /
- Dictyophora echinovolvata
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图 3 碳量子点CDs的荧光特性(A)，激发/发射光谱(B)和波长依赖性发射(C)
Figure 3. Fluorescence (A), exciation/emission sectra (B), and wave length-dependent emission (C) of CDs
图 4 碳量子点对青枯雷尔氏菌FJAT-91(A)和地毯草黄单胞菌FJAT-10151(B)的抑制作用
Figure 4. Inhibitory effects of CDs on R. solanacearum (A) and X. axonopodis (B)
表 1 碳量子点对植物病原菌的抑菌作用
Table 1. Inhibitory effects of CDs on pathogens
(单位/mm) 菌株
Strains抑菌圈直径Inhibition zone diameters 平均值±标准差
Mean±SD1 2 3 4 5 6 FJAT-91 22.25 26.64 24.18 25.31 26.23 26.65 25.21±1.73 FJAT-10151 21.79 21.64 21.14 23.68 23.55 22.64 22.41±1.05 
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