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西藏尼玛县盐碱地嗜碱芽胞杆菌资源采集与鉴定

刘国红 刘波 陈倩倩 车建美 阮传清 陈峥

引用本文:
Citation:

西藏尼玛县盐碱地嗜碱芽胞杆菌资源采集与鉴定

    作者简介: 刘国红(1983-),博士,助理研究员,主要从事微生物生物技术与农业生物药物研究(E-mail:1iuguohong624@sina.com)
    通讯作者: 刘波(1957-),博士、研究员,主要从事微生物生物技术与农业生物药物研究(E-mail:fzliubo@163.com)
  • 基金项目:

    国家自然科学基金(31370059);福建省种业创新与产业化工程项目(FJZZZY-1544);福建省种业创新项目(2015);福建省农业科学院博士基金项目(2014BS-3);福建省农业科学院青年英才计划(YJRC2015-17)

  • 中图分类号: Q939

Collection and Identification of Bacillus Species in Saline-alkali Habitatsat Nima County, Tibet

  • 摘要: 为调查西藏尼玛县盐碱地环境芽胞杆菌资源,采用纯培养法从西藏尼玛县采集的盐碱地样品中分离芽胞杆菌,利用16S rRNA基因进行系统发育进化分析。同时,选取代表性菌株进行生理生化特性分析。结果筛选到了14株芽胞杆菌,经16S rRNA基因鉴定均属于嗜碱芽胞杆菌。16S rRNA系统发育分析表明,14株嗜碱菌划分为3个群:类群I包含11株菌,与假坚强芽胞杆菌Bacillus pseudofirmus的亲缘关系较近,类群Ⅱ由FJAT-24879组成,与Jeotgalibacillus campisalis的亲缘关系较近;类群Ⅲ包含2株菌,与Bacillus horikoshii具有较近的亲缘关系。3种芽胞杆菌均在pH8以上生长良好,生理生化结果分析表明嗜碱芽胞杆菌杆菌类群具有丰富的生理生化特征。研究结果表明西藏尼玛县盐碱地含有较多芽胞杆菌种类。
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