摘要:
为研究羽毛肽粉替代鱼粉对瓦氏黄颡鱼Pelteobagrus vachelli幼鱼生长、体成分和消化酶活力的影响，以基础饲料为对照，分别在基础饲料中添加5%、10%、15%、20%羽毛肽粉，饲喂初始体重（252）g试验鱼60 d。结果表明，试验黄颡鱼增重率、特定生长率随羽毛肽粉添加水平升高呈先升后降趋势，添加水平为10%时，增重率达到最大值117.48%。当添加水平为15%、20%时，增重率、特定生长率显著下降（P0.05）；摄食总量随着羽毛肽粉的添加水平也呈先升后降趋势，在10%时，摄食总量达到最大值。饲料系数随着羽毛肽粉添加水平增加呈升高趋势，5%添加组与对照组无显著性差异（P0.05），其余各组均显著高于对照组（P0.05）；羽毛肽粉在20%添加水平内对瓦氏黄颡鱼幼鱼体蛋白质、灰分、脂肪、水分无显著性影响（P0.05）；试验黄颡鱼肝胰腺胰蛋白酶活力随羽毛肽粉添加水平的升高呈升高趋势，5%添加水平组与对照组无显著差异（P0.05），但显著低于其余各组（P0.05）；试验黄颡鱼肠胰蛋白酶活力与肝胰腺蛋白酶活力呈相似的变化趋势，当添加水平达15%时，肠胰蛋白酶活力与胰胰蛋白酶活力比值显著下降（P0.05）；不同添加水平羽毛肽粉对试验黄颡鱼淀粉酶活性无显著性影响（P0.05）；5%羽毛肽粉添加组的肠、肝胰腺脂肪酶活力最低，显著低于其他羽毛肽粉添加组与对照组（P0.05）；羽毛肽粉添加水平至10%时，肠脂肪酶活力与对照组无显著性差异（P0.05），至15%时，肝胰脏脂肪酶活力与对照组无显著差异（P0.05）；羽毛肽粉添加组瓦氏黄颡鱼肠胃蛋白酶活性显著高于对照组（P0.05），15%、20%添加组5%、10%添加组对照组，各组间肝胰腺胃蛋白酶活性无显著性差异。在瓦氏黄颡鱼饲料中可以适量添加羽毛肽粉，添加水平在10%以内时，可以显著提高瓦氏黄颡鱼摄食量，促进其生长。
关键词:
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瓦氏黄颡鱼 /
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幼鱼 /
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羽毛肽粉 /
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生长 /
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体成分 /
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消化酶 /
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营养
Abstract:
Effects of peptide meal made from feathers in the feed on the growth, body composition, and digestive enzymes of the darkbarbel catfish, Pelteobagrus vachelli, were studied. Triplicate groups of 30 fish with an averaging weight of 25(2 gin each group were randomly selected to be fed ondiets formulated with the addition of 0, 5, 10, 15, or 20% of the peptide meal for 60 days. The results showed that(1) the peptide meal significantly affected the weight gain rate(WGR), specific growth rate(SGR), and total feed intake(TFI) on the fish (P0.05); (2) a 10% addition of the meal in the feed was determined to be appropriatefora diet that produced a WGR of 117.48% on the fish; (3) the WGR and SGR of the fish decreased significantly when the meal addition increased to 15% or 20%; (4) with increasing amount of the peptide meal, the feed conversation ratio increased gradually; (5)no significant differences were found among all groups on the muscle moisture, lipid, protein,or ash content on the fish fed on diet with 20% peptide meal addition (P0.05); (6)the trypsinactivity in thehepatopancreas of the fishsignificantly increased with increasing meal addition (P0.05), but no significant differences were found between 5% meal addition and control; (7) the trypsin activities in the intestine and hepatopancreas showed a similar trend, however, the ratio of intestinal trypsin/hepatopancreatic trypsinsignificantly decreased at 15% meal addition; (8) no significant differences was observedon the amylase activities among all groups (P0.05); (9) at 5% meal addition, the lipase activity in the intestine or hepatopancreas of the fish was lowest among all groups (P0.05); (10)with a 10% meal addition, there was no significant differenceobserved in the intestinal lipase activity, and the hepatopancreatic lipase activityremain same as control up to 15% of the meal addition; (11)the pepsin activities in the stomach of the fishfed with different diets were significant higher than control, and the 15% and 20% meal addition were higher than 5%, while 10% addition was higher than control (P0.05); (12)no significant differences were evidenton the stomach pepsin activities in the hepatopancreas of the fish from all groups. It appeared that a 10% addition of the feather peptide meal to the basal diet could be satisfactorily applied for feeding juvenile darkbarbel catfish.
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