摘要:
采用盆栽试验研究土壤中不同施芘水平对菠菜(Spinacia oleracea L.)生长、叶片叶绿素和抗氧化酶活性的影响。结果表明:50～200 mg•kg-1施芘水平下菠菜幼苗叶片的各项叶绿体色素含量、不同时期的根长与对照均无显著差异;施芘浓度大于50 mg•kg-1,菠菜苗期的株高、根和茎叶的鲜重显著降低;芘大于100mg•kg-1时,菠菜收获期的根和茎叶干重显著受到抑制;芘胁迫导致菠菜幼苗叶片的丙二醛累积量提高,可溶性糖含量增加;同时叶片的APX、CAT、POD活性和非酶抗氧化剂AsA含量均随芘浓度增加而显著增加。
关键词:
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菠菜 /
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芘胁迫 /
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生长 /
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叶绿素 /
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抗氧化酶
Abstract:
An experiment was conducted in flowerpots to study the effect of the pyrene level in soil on the growth leaf,chlorophyll content and anti-oxidative enzyme activity of the spinach(Spinacia oleracea L.).No significant differences were found on the root length in different growth periods or the contents of chlorophyll a,chlorophyll b,chlorophyll a+b and carotenoid in the leaves,when 50-200 mg•kg-1 pyrene was applied.However,when pyrene application was greater than 50 mg•kg-1,significant reductions on plant height,fresh root,stem and leaf weights at the seedling stage were observed.As the pyrene increased beyond 100 mg•kg-1,the dry root,stem and leaf weights at harvesting stage decreased significantly.Pyrene stress increased MDA and SS in the leaves of the spinach seedling.It also significantly increased the APX,CAT and POD activities,as well as the antioxidant AsA contents in the leaves.
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