山药块茎生长期5种内源激素含量变化对块茎膨大的影响

敖兰吉亚; 季祥; 邵盈; 王志敏; 赵令敏; 张艳芳; 霍秀文

doi:10.19303/j.issn.1008-0384.2019.03.005

山药块茎生长期5种内源激素含量变化对块茎膨大的影响

doi: 10.19303/j.issn.1008-0384.2019.03.005

内蒙古农业大学农学院/内蒙古野生蔬菜种质资源与创新重点实验室, 内蒙古呼和浩特 010019

基金项目:

国家自然科学基金项目 31360480

详细信息

作者简介:
敖兰吉亚(1994-), 女, 硕士研究生, 研究方向:蔬菜遗传育种与生物技术(E-mial:1140842979@qq.com)

通讯作者:
霍秀文(1968-), 女, 教授, 博士生导师, 研究方向:蔬菜遗传育种与生物技术(E-mail:huoxiuwen@imau.edu.cn.com)

中图分类号: S632.1
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- 被引次数: 0
出版历程
- 收稿日期: 2018-12-30
- 修回日期: 2019-01-25
- 刊出日期: 2019-03-28

Correlation Between Endogenous Hormones and Tuber Growth of Dioscorea opposite Thunb.

College of Agriculture, Inner Mongolia Agricultural University/Inner Mongolia Wild Vegetable Germplasm Resources and Innovation Key Laboratory, Hohhot, Inner Mongolia 010019, China

摘要

摘要: 目的探讨山药不同生长发育阶段主要内源激素含量的变化规律，以及不同山药品种形态指标与5种内源激素的相关性，为进一步研究山药生长发育的生理机制和指导山药的高产优质栽培奠定基础。方法以毕克齐山药和河北安平白山药7个不同生长发育时期块茎为材料，采用酶联免疫吸附法分别测定了脱落酸（ABA）、赤霉素（GA₃）、生长素（IAA）、茉莉酸（JA）、玉米素（ZR）等5种内源激素含量，并分析不同生长期内源激素含量的动态变化，以及山药形态指标与内源激素的相关性。结果在两个山药品种块茎膨大的生长发育过程中，内源激素IAA和ABA含量总体呈逐渐上升的趋势；内源激素ZR和GA₃含量有变化，但比较稳定；内源激素JA含量，两个品种均呈下降趋势，尤其是河北安平白山药的JA含量与形态指标呈极显著的负相关，说明JA明显抑制了河北安平白山药块茎的周长增加、重量增加及伸长生长；内源激素IAA和JA在不同生长期的含量水平高低，两个品种表现出不同的变化趋势。结论内源激素ABA、GA₃、IAA可促进2个山药品种块茎生长发育，其中ABA、IAA对毕克齐山药块茎伸长的促进作用更显著；而内源激素ZR、JA可促进河北安平白山药的块茎形成及初期块茎周长的增加。
- 山药 /
- 块茎膨大周期 /
- 内源激素 /
- 形态指标 /
- 相关性
Abstract: Objective Physiology relating to the hormones of two varieties of Dioscorea opposite was studied for cultivation improvement. Method The endogenous ABA, GA₃, IAA, JA, and ZR in slim-type Inner Mongolia Bikeqi yam and short-type Hebeianpingbai yam were determined by using ELISA at 7 tuber development stages. The dynamic changes of the hormones were correlated with the morphological indicators of the tuber growth. Result For both varieties, IAA and ABA increased gradually but not significantly during the tuber development. ZR and GA₃ did not change significantly. And, JA declined showing retarded extensions on tuber diameter, weight and length in Hebeianpingbai yam. Conclusion ABA, GA₃ and IAA promoted the tuber development on both varieties; ABA and IAA enhanced the tuber elongation on Inner Mongolia Bikeqi; while ZR and JA stimulated the tuber formation and diameter increase on Hebeianpingbai yam at the beginning stage.
- Dioscorea opposite Thunb. /
- tuber development /
- endogenous hormones /
- relevance /

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图 1 ABA在山药不同生长期含量的变化

Figure 1. Changes on ABA at various growth stages of yams

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图 2 GA₃在山药不同生长期含量的变化

Figure 2. Changes on GA₃ at various growth stages of yams

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图 3 IAA在山药不同生长期含量的变化

Figure 3. Changes on IAA at various growth stages of yams

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图 4 JA在山药不同生长期含量的变化

Figure 4. Changes on JA at various growth stages of yams

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图 5 ZR在山药不同生长期含量的变化

Figure 5. Changes on ZR at various growth stages of yams

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表 1 不同山药品种在相同生长发育时期5种内源激素的差异

Table 1. Variations on 5 endogenous hormones in D. opposite at a same development stage

生长期 Growth period	品种 Varieties	ABA/ (ng·g^-1)	GA₃/ (ng·g^-1)	IAA/ (ng·g^-1)	JA/ (ng·g^-1)	ZR/ (ng·g^-1)
块茎膨大始期 Initial period of tuber expansion	河北安平白山药 Hebeianpingbai yam	39.70±1.89bB	5.46±0.35aA	32.09±1.88bA	24.70±0.56aA	7.06±0.29aA
	毕克齐山药 Bikeqi yam	55.83±3.13aA	5.33±0.22aA	35.94±0.90aA	19.92±0.48bB	5.80±0.37bA
块茎膨大初期 The beginning of tuber expansion	河北安平白山药 Hebeianpingbai yam	32.78±1.53bB	4.39±0.10bA	40.52±0.97aA	20.35±0.12bB	10.86±0.53aA
	毕克齐山药 Bikeqi yam	39.21±1.07aA	5.86±0.39aA	27.44±0.60bB	37.97±1.93aA	6.53±0.23bB
气生块茎期 Air tuber stage	河北安平白山药 Hebeianpingbai yam	38.01±1.78aA	5.12±0.23aA	27.90±1.11bA	17.64±0.28bB	5.77±0.23bB
	毕克齐山药 Bikeqi yam	32.70±0.80bA	5.59±0.36aA	31.60±1.22aA	20.78±0.46aA	7.95±0.16aA
块茎膨大中期 Intermediate period of tuberexpansion	河北安平白山药 Hebeianpingbai yam	34.30±0.97bA	5.56±0.26aA	33.78±0.95bB	11.70±0.38bB	6.66±0.25aA
	毕克齐山药 Bikeqi yam	38.518±1.76aA	5.99±0.30aA	39.28±1.24aA	20.83±0.73aA	7.15±0.26aA
块茎膨大盛期 The vigorous period of tuber expansion	河北安平白山药 Hebeianpingbai yam	30.06±2.20bB	5.08±0.24aA	36.19±1.16aA	11.25±0.57aA	7.10±0.44aA
	毕克齐山药	43.71±2.27aA	3.87±0.09bB	36.07±1.30aA	10.81±0.25aA	5.68±0.35bA
块茎膨大后期 The later period of tuber expansion	河北安平白山药 Hebeianpingbai yam	54.98±3.48aA	4.97±0.26aA	55.76±2.93aA	10.85±0.17bB	7.50±0.34aA
	毕克齐山药 Bikeqi yam	43.20±1.47bA	4.03±0.12bA	35.40±0.96bB	15.90±0.53aA	6.49±0.48bA
成熟收获期 Mature harvest period	河北安平白山药 Hebeianpingbai yam	70.95±4.13bB	5.03±0.30bA	41.02±1.94bB	12.20±0.51aA	5.81±0.26bB
	毕克齐山药 Bikeqi yam	100.33±7.53aA	5.76±0.28aA	68.23±1.24aA	12.42±0.53aA	7.42±0.11aA
注：表中数据后无相同小写字母者表示在P＜0.05水平上差异显著; 无相同大写字母者表示在P < 0.01水平上差异极显著。 Note: Data with different lowercase letters indicate significant differences at P < 0.05; data with different capital letters indicate extremely significant at P < 0.01.

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表 2 河北安平白山药与5种内源激素的相关性

Table 2. Correlation between tuber growth and 5 endogenous hormones of Hebeianpingbai yam

项目 Progects	块茎长度 Tuber length	块茎周长 Tuber circumference	块茎干重 Tuber dry weight	块茎鲜重 Tuber freshweight	JA	IAA	ZR	GA₃	ABA
块茎长度 Tuber length		0.892^**	0.961^**	0.862^*	-0.951^**	0.587	-0.269	-0.109	0.389
块茎周长 Tuber circumference			0.852^*	0.898^**	-0.874^*	0.642	-0.092	-0.273	0.561
块茎干重 Tuberdry weight				0.927^**	-0.937^**	0.504	-0.447	-0.006	0.504
块茎鲜重 Tuber freshweight					-0.889^**	0.59	-0.383	0.013	0.671
JA						-0.45	0.34	-0.072	-0.312
IAA							0.3	-0.416	0.502
ZR								-0.729	-0.389
GA₃									-0.041
ABA
注：表中数据上标*表示在0.01级别(双尾)相关极显著；表示在0.05级别(双尾)相关显著。表 3同。 Note: ** represents extremely significant correlation at P < 0.01; * represents significant correlation at P < 0.05.The same as table 3.

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表 3 毕克齐山药与5种内源激素的相关性

Table 3. Correlation between tuber growth and 5 endogenous hormones of Inner Mongolia Bikeqi yam

项目 Progects	块茎长度 Tuber length	块茎周长 Tuber circumference	块茎干重 Tuber dry weight	块茎鲜重 Tuber freshweight	JA	IAA	ZR	GA₃	ABA
块茎长度 Tuber length		0.558	0.653	0.636	-0.364	0.322	0.145	-0.435	0.155
块茎周长 Tuber circumference			0.854^*	0.989^**	-0.446	0.131	-0.237	-0.38	-0.059
块茎干重 Tuberdry weight				0.876^**	-0.539	0.13	-0.309	-0.665	0.028
块茎鲜重 Tuber
freshweight					-0.525	0.196	-0.256	-0.46	0.008
JA						-0.546	0.069	0.539	-0.412
IAA							0.303	0.193	0.942^**
ZR								0.588	0.119
GA₃									0.181
ABA

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山药块茎生长期5种内源激素含量变化对块茎膨大的影响

doi: 10.19303/j.issn.1008-0384.2019.03.005

作者简介:
敖兰吉亚(1994-), 女, 硕士研究生, 研究方向:蔬菜遗传育种与生物技术(E-mial:1140842979@qq.com)

通讯作者:
霍秀文(1968-), 女, 教授, 博士生导师, 研究方向:蔬菜遗传育种与生物技术(E-mail:huoxiuwen@imau.edu.cn.com)

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Correlation Between Endogenous Hormones and Tuber Growth of Dioscorea opposite Thunb.

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山药块茎生长期5种内源激素含量变化对块茎膨大的影响

doi: 10.19303/j.issn.1008-0384.2019.03.005

作者简介: 敖兰吉亚(1994-), 女, 硕士研究生, 研究方向:蔬菜遗传育种与生物技术(E-mial:1140842979@qq.com)

通讯作者: 霍秀文(1968-), 女, 教授, 博士生导师, 研究方向:蔬菜遗传育种与生物技术(E-mail:huoxiuwen@imau.edu.cn.com)

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Correlation Between Endogenous Hormones and Tuber Growth of Dioscorea opposite Thunb.

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出版历程

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作者简介:
敖兰吉亚(1994-), 女, 硕士研究生, 研究方向:蔬菜遗传育种与生物技术(E-mial:1140842979@qq.com)

通讯作者:
霍秀文(1968-), 女, 教授, 博士生导师, 研究方向:蔬菜遗传育种与生物技术(E-mail:huoxiuwen@imau.edu.cn.com)