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甘蓝型油菜抗除草剂恢复系18Z363苯磺隆抗性剂量筛选

张超 代文东 王军 唐容 黄莎 杨克相 饶勇

张超,代文东,王军,等. 甘蓝型油菜抗除草剂恢复系18Z363苯磺隆抗性剂量筛选 [J]. 福建农业学报,2019,34(9):1081−1086. doi: 10.19303/j.issn.1008-0384.2019.09.013
引用本文: 张超,代文东,王军,等. 甘蓝型油菜抗除草剂恢复系18Z363苯磺隆抗性剂量筛选 [J]. 福建农业学报,2019,34(9):1081−1086. doi: 10.19303/j.issn.1008-0384.2019.09.013
ZHANG C, DAI W D, WANG J, et al. Tribenuron-methyl Tolerance of Brassica napus Restorer Line 18Z363 [J]. Fujian Journal of Agricultural Sciences,2019,34(9):1081−1086. doi: 10.19303/j.issn.1008-0384.2019.09.013
Citation: ZHANG C, DAI W D, WANG J, et al. Tribenuron-methyl Tolerance of Brassica napus Restorer Line 18Z363 [J]. Fujian Journal of Agricultural Sciences,2019,34(9):1081−1086. doi: 10.19303/j.issn.1008-0384.2019.09.013

甘蓝型油菜抗除草剂恢复系18Z363苯磺隆抗性剂量筛选

doi: 10.19303/j.issn.1008-0384.2019.09.013
基金项目: 贵州省科技计划项目(黔科合NY[2015]3019-1号);贵州省科学技术基金项目(黔科合LH字[2014]7685号);贵州省油菜现代农业产业技术体系建设项目(GZCYTX2018-0801)
详细信息
    作者简介:

    张超(1985−),男,硕士研究生,助理研究员,研究方向:油菜遗传育种(E-mail:18083606406@163.com

    通讯作者:

    饶勇(1959−),男,研究员,研究方向:油菜遗传育种与应用推广(E-mail:ry590@21cn.com

  • 中图分类号: S 482.4

Tribenuron-methyl Tolerance of Brassica napus Restorer Line 18Z363

  • 摘要:   目的  研究不同剂量苯磺隆对甘蓝型油菜苗期叶绿素含量、存活率及成熟期农艺性状的影响,为波里马细胞质不育(polima cytoplasmic male sterility, pol CMS)恢复系18Z363筛选安全、有效、经济的苯磺隆除草剂施用量提供依据。  方法  以pol CMS恢复系18Z363、M342、2350C及杂交种LDX95-58A×18Z363为试验材料,盆栽种植于4-6叶期喷施不同剂量的苯磺隆,7 d后采用SPAD-502叶绿素测定仪测量叶片的叶绿素含量,14 d后调查存活率;大田种植4-6叶期喷施不同剂量的苯磺隆,于成熟期考察主要农艺性状。  结果  油菜叶片叶绿素含量SPAD值随苯磺隆施用剂量的增加而下降,在18.0 g·hm−2及以上剂量处理下,不抗除草剂材料2350C叶片叶绿素含量SPAD值显著下降到较低水平,而抗除草剂材料18Z363、M342及杂交种LDX95-58A×18Z363的SPAD值仍保持较高水平;18.0 g·hm−2处理下不抗除草剂材料2350C存活率为0,而18Z363、M342及杂交种LDX95-58A×18Z363存活率在88.9%以上;高于18.0 g·hm−2剂量的苯磺隆处理对18Z363、M342及杂交种LDX95-58A×18Z363成熟期的主要农艺性状造成不同程度的负面影响。  结论  pol CMS恢复系18Z363及配制的“三系”杂交种的最适苯磺隆施用剂量为18.0 g·hm−2
  • 表  1  苯磺隆各处理施药量

    Table  1.   Dosages of tribenuron-methyl applied for treatments

    处理
    Treatments
    苯磺隆质量浓度
    Concentration of tribenuron-methyl/(mg·L−1
    苯磺隆用量
    Application dosage of tribenuron-methyl /(g·hm−2)
    施用有效成分量
    Dose of tribenuron-methyl /(g·hm−2)
    CK00.00.0
    T120090.09.0
    T2400180.018.0
    T3600270.027.0
    T4800360.036.0
    下载: 导出CSV

    表  2  不同剂量苯磺隆处理后叶片叶绿素含量SPAD值

    Table  2.   Chlorophyll SPAD in leaves of rapeseed plants being sprayed with tribenuron-methyl at various dosages

    处理
    Treatments
    叶绿素含量SPAD值 The chlorophyll SPAD value
    2350CM34218Z363LDX95-58A×18Z363
    CK42.7±1.7a44.2±1.7a41.8±1.3a45.4±1.8a
    T139.2±1.1b42.6±1.4ab39.7±0.5ab42.8±0.9b
    T222.7±0.8c40.3±0.6b38.4±0.6b38.2±1.0c
    T319.3±1.3d35.9±1.2c35.7±1.3c33.6±1.3d
    T416.9±1.0d34.0±1.7c32.5±2.3c26.5±1.5e
    注:同一列不同小写字母表示显著差异(P<0.05),表3、4同。
    Note:The different small letters in the same column represent significances difference (P<0.05),the same as Table 3 - 4.
    下载: 导出CSV

    表  3  不同剂量苯磺隆处理后油菜的存活率

    Table  3.   Survival rate of rapeseed plants being sprayed with tribenuron-methyl at various dosages

    处理
    Treatments
    存活率 Survival rate/%
    2350CM34218Z363LDX95-58A×18Z363
    CK 100.0±0.0a 100.0±0.0a 100.0±0.0a 100.0±0.0a
    T1 57.8±5.1b 98.9±1.9a 96.7±3.3a 93.3±3.3b
    T2 0.0±0.0c 97.8±1.9a 93.3±3.3ab 88.9±3.8b
    T3 0.0±0.0c 86.7±3.3b 85.6±5.1b 78.9±5.1c
    T4 0.0±0.0c 73.3±6.7c 75.6±5.1c 55.6±1.9d
    下载: 导出CSV

    表  4  不同剂量苯磺隆处理对油菜成熟期主要农艺性状的影响

    Table  4.   Effectsof main agronomic traitsunder different dose of tribenuron-methyltreatments

    材料
    Material
    处理
    Treatments
    株高
    Plant
    height
    /cm
    分枝高
    Branch
    height
    /cm
    一次
    分枝数
    First
    branch
    number
    二次
    分枝数
    Second
    branch
    number
    含油量
    Oil
    content
    /%
    每角粒数
    Seeds per pod
    千粒重
    Thousand seed
    weight /g
    单株角果数
    Number of pods per
    plant
    主花序长
    Length of main inflorescence /cm
    主花序角果数
    Number of pods per main inflorescence
    单株产量
    Yield per plant /g
    18Z363CK180.6±4.4 a66.6±6.3 b8.6±0.6 a15.3±1.1 a44.3±0.9 a17.7±1.0 a3.3±0.4 a342.5±19.0 a62.5±4.5 a77.8±5.2 a17.7±0.8 a
    T1181.1±4.3 a65.2±9.8 b9.0±0.6 a15.9±1.6 a45.3±2.4 a17.3±1.2 a3.4±0.2 a336.1±13.2 a62.2±4.2 a75.2±8.0 a17.7±2.9 a
    T2180.4±3.9 a69.1±3.9 b8.9±0.6 a15.5±1.2 a43.7±3.0 a17.5±2.1 a3.3±0.3 a335.3±19.0 a61.4±3.3 ab73.1±6.0 a16.5±3.2 a
    T3178.2±2.6 a71.1±2.7 ab7.1±0.3 b11.1±0.8 b44.3±2.1 a17.0±1.0 a3.4±0.3 a277.9±8.6 b58.5±6.3 ab67.0±5.8 a14.0±2.5 ab
    T4168.6±3.1 b81.2±2.0 a6.6±0.5 b8.8±0.6 c44.1±2.4 a16.8±1.4 a3.2±0.3 a251.6±19.4 b52.4±4.0 b53.0±3.8 b11.2±0.2 b
    M342CK183.1±4.8 a69.6±5.9 b7.7±0.9 a5.5±0.7 a43.7±1.0 a22.2±0.7a3.7±0.1 a286.0±21.5 a61.7±3.6 a61.3±5.7 ab19.5±0.3 a
    T1182.5±3.9 a69.3±4.0 b7.7±0.4 a5.7±0.4 a44.1±1.1 a22.0±1.4 a3.7±0.1 a275.1±19.4 a60.7±6.8 a64.2±6.9 a19.1±2.4 ab
    T2178.3±4.9 ab73.4±4.3 ab6.9±0.7 a4.8±0.7 ab43.4±1.7 a22.7±1.5 a3.7±0.2 a260.5±23.6 ab58.7±5.3 a59.7±3.8 ab18.8±1.2 ab
    T3177.9±3.6 ab79.2±4.7 a6.8±0.4 a4.3±0.6 bc42.6±1.5 a22.6±2.5 a3.6±0.2 a231.9±24.2 bc53.0±4.0 ab54.0±4.0 b16.4±1.5 bc
    T4171.7±4.3 b80.3±5.4 a5.6±0.4 b3.4±0.6 c42.4±1.4 a21.9±1.6 a3.6±0.2 a201.0±15.5 c46.3±5.2 b43.2±3.7 c14.1±0.9 c
    LDX95-58Ax18Z363CK206.4±7.5 a102.7±8.9 a8.1±0.5 ab14.3±1.0 a40.4±0.6 b16.3±1.1 a3.4±0.2 a352.2±48.8 a68.9±5.6 ab81.5±4.0 a18.4±0.5 a
    T1203.4±6.9 ab101.6±5.5 a8.2±0.4 ab13.9±1.5 a41.5±1.3 ab15.8±1.7 a3.3±0.2 a363.3±37.3 a73.2±4.6 a78.6±6.9 a17.6±1.0 ab
    T2203.1±7.1 ab99.7±7.8 a8.3±0.8 a13.5±1.2 a40.8±1.3 ab15.2±2.6 a3.3±0.2 a361.5±25.1 a71.9±4.1 a79.3±7.6 a15.7±1.4 bc
    T3197.0±7.3 ab104.2±6.0 a7.4±0.4 bc10.9±0.7 b42.7±0.5 a16.4±2.7 a3.4±0.1 a312.6±22.7 ab63.5±3.3 b65.3±6.0 b14.7±1.1 cd
    T4191.4±5.1 b102.9±7.6 a6.5±0.3 c7.8±0.6 c41.2±1.1 ab16.2±2.2 a3.3±0.2 a282.9±22.6 b54.3±2.9 c52.7±5.5 c12.9±1.6 d
    下载: 导出CSV
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  • 收稿日期:  2019-06-29
  • 修回日期:  2019-09-01
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