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嫁接对大果番茄果实挥发性风味物质的影响

刘子记 刘维侠 牛玉 杨衍

刘子记,刘维侠,牛玉,等. 嫁接对大果番茄果实挥发性风味物质的影响 [J]. 福建农业学报,2020,35(11):1207−1214 doi: 10.19303/j.issn.1008-0384.2020.11.005
引用本文: 刘子记,刘维侠,牛玉,等. 嫁接对大果番茄果实挥发性风味物质的影响 [J]. 福建农业学报,2020,35(11):1207−1214 doi: 10.19303/j.issn.1008-0384.2020.11.005
LIU Z J, LIU W X, NIU Y, et al. Effect of Plant Grafting on Flavoring Volatiles in Large-fruit Tomatoes [J]. Fujian Journal of Agricultural Sciences,2020,35(11):1207−1214 doi: 10.19303/j.issn.1008-0384.2020.11.005
Citation: LIU Z J, LIU W X, NIU Y, et al. Effect of Plant Grafting on Flavoring Volatiles in Large-fruit Tomatoes [J]. Fujian Journal of Agricultural Sciences,2020,35(11):1207−1214 doi: 10.19303/j.issn.1008-0384.2020.11.005

嫁接对大果番茄果实挥发性风味物质的影响

doi: 10.19303/j.issn.1008-0384.2020.11.005
基金项目: 海南省科技计划项目(ZDYF2020055);中国热带农业科学院基本科研业务费专项项目(1630032017027);农业农村部财政专项项目(NFZX2018)
详细信息
    作者简介:

    刘子记(1982−),男,博士,副研究员,研究方向:蔬菜遗传育种(E-mail:liuziji1982@163.com

    通讯作者:

    杨衍(1971−),男,博士,研究员,研究方向:蔬菜遗传育种(E-mail:catasvegetable@163.com

  • 中图分类号: S 641.2

Effect of Plant Grafting on Flavoring Volatiles in Large-fruit Tomatoes

  • 摘要:   目的  探索嫁接对大果番茄果实挥发性风味物质的影响,为进一步开展大果番茄风味调控研究提供依据。  方法  采用顶空固相微萃取-气相色谱-质谱联用技术对博收1号、辉腾和辉腾2号3个大果番茄品种实生苗(S)和嫁接苗(J)果实的挥发性风味物质种类和相对含量进行分析。  结果  博收1号S含有59种挥发性物质,包括7种特有成分;博收1号J含有62种挥发性物质,包括10种特有成分。辉腾S含有59种挥发性物质,包括8种特有成分;辉腾J含有64种挥发性物质,包括13种特有成分。辉腾2号S含有58种挥发性物质,包括7种特有成分;辉腾2号J含有60种挥发性物质,包括9种特有成分。  结论  综合比较3个大果番茄品种发现,3个大果番茄品种的嫁接苗(J)均能显著提高果实中6-甲基-5-庚烯-2-酮、β-紫罗兰酮和E-2-己烯醛的含量。
  • 图  1  挥发性物质总离子流图

    注:A:博收1号S;B:博收1号J;C:辉腾S;D:辉腾J;E:辉腾2号S;F:辉腾2号J

    Figure  1.  Total ionic chromatograms of volatiles in tomatoes

    Note: A: Boshou No. 1S; B: Boshou No. 1J; C: Huiteng S; D: Huiteng J; E: Huiteng No. 2S; F: Huiteng No. 2J

    表  1  嫁接和非嫁接大果番茄品种挥发性物质及相对含量

    Table  1.   Volatiles and relative contents in fruits on grafted and non-grafted large-fruit varieties of tomato plants

    序号
    No.
    类别
    Category
    保留时间
    Retention  
    time/min
    化合物名称
    Compound
    分子式
    Formula
    相对含量
    Relative content/%
    博收1号 S
    Boshou
    No.1 S
    博收1号 J
    Boshou
    No.1 J
    辉腾 S
    Huiteng
    S
    辉腾 J
    Huiteng
    J
    辉腾2号 S
    Huiteng
    No.2 S
    辉腾2号 J
    Huiteng
    No.2 J
    醇类 Alcohol
    1 1.64 乙醇 Ethanol C2H6O 1.55 0.09 0.57 0.34 0.55 1.42
    2 2.54 异丁醇 Isobutyl alcohol C4H10O 0.05 0.01 0.10 0.04 0.12
    3 3.01 1-丁醇 1-Butanol C4H10O 0.08 0.03 0.04 0.06 0.02 0.19
    4 4.30 3-甲基-1-丁醇 3-Methyl-1-butanol C5H12O 2.66 0.29 1.90 1.69 0.57 2.45
    5 4.36 2-甲基-1-丁醇 2-Methyl-1-butanol C5H12O 7.00 0.44 3.92 1.33 1.04 5.34
    6 5.15 1-戊醇 1-Pentanol C5H12O 2.02 0.65 3.19 1.06 1.64 2.64
    7 8.15 Z-3-已烯醇 Z-3-Hexenol C6H12O 1.82 7.33 2.54 1.89 1.92 5.03
    8 8.73 1-己醇 1-Hexanol C6H14O 2.86 4.22 0.87 1.34 1.89 3.08
    9 13.28 1-庚醇 1-Heptanol C7H16O 0.32 0.38 0.14 0.23 0.28 0.29
    10 13.36 1-辛烯-3-醇 1-Octen-3-ol C8H16O 0.64 0.62 0.36 0.60 0.61 0.57
    11 14.34 6-甲基-5-庚烯-2-醇 6-Methyl-5-hepten-2-ol C8H16O 0.40 0.18 0.41 0.24 0.38
    12 16.09 2-乙基己醇 2-Ethylhexanol C8H18O 2.26 0.81 1.62
    13 16.83 苯甲醇 Benzenemethanol C7H8O 0.02
    14 18.16 1-辛醇 1-Octanol C8H18O 1.36 2.67 1.03 1.48 1.92 1.53
    15 20.35 苯乙醇 Benzeneethanol C8H10O 0.84 0.06 0.52 0.70 0.56
    16 23.02 1-壬醇 1-Nonanol C9H20O 0.22 0.57 0.22 0.09 0.25 0.52
    17 26.96 Z-3-癸烯-1-醇 Z-3-Decen-1-ol C10H20O 0.29
    酮类 Ketone
    18 3.25 1-戊烯-3-酮 1-Penten-3-one C5H8O 2.30 0.56 2.58 1.18 2.46 3.71
    19 13.79 6-甲基-5-庚烯-2-酮 6-Methyl-5-hepten-2-one C8H14O 4.54 6.03 4.08 7.61 3.85 5.49
    20 35.19 香叶基丙酮 Geranyl acetone C13H22O 1.05 1.57 0.41 1.31 0.89 0.57
    21 36.54 β-紫罗兰酮 β-Ionone C13H20O 0.04 0.11 0.09 0.17 0.08 0.17
    酯类 Ester
    22 1.86 乙酸甲酯 Methyl acetate C3H6O2 0.05 0.14 0.56 0.18 0.41 0.07
    23 2.38 乙酸乙酯 Ethyl acetate C4H8O2 10.42 0.15 3.81 0.80 2.47 7.50
    24 2.55 丙酸甲酯 Methyl propanoate C4H8O2 0.05
    25 2.90 乙酸异丙酯 Isopropyl acetate C5H10O2 0.46 0.41 0.44
    26 3.70 丙酸乙酯 Ethyl propanoate C5H10O2 0.03
    27 3.75 乙酸丙酯 Propyl acetate C5H10O2 0.22 0.03 0.03 0.13
    28 6.46 乙酸丁酯 Butyl acetate C6H12O2 0.06 0.03 0.16
    29 8.87 乙酸-2-甲基丁酯 2-Methylbutyl acetate C7H14O2 1.31 1.71 0.40 0.65 1.62
    30 10.42 乙酸戊酯 Amyl acetate C7H14O2 0.03
    31 14.77 Z-乙酸-3-己烯醇酯 Z-3-Hexenyl Acetate C8H14O2 0.58 0.07
    32 15.10 乙酸己酯 Hexyl acetate C8H16O2 0.07 0.17
    33 21.74 乙酸-2-乙基己醇酯 2-Ethylhexyl acetate C10H20O2 0.17
    34 23.95 水杨酸甲酯 Methyl salicylate C8H8O3 0.89 2.39 2.18 20.06 0.97 1.50
    醛类 Aldehyde
    35 1.99 异丁醛 Isobutanal C4H8O 0.01
    36 2.06 2-甲基-2-丙烯醛 2-Methyl-2-propenal C4H6O 0.01 0.02
    37 2.20 丁醛 Butanal C4H8O 0.01 0.03 0.04 0.02
    38 2.79 3-甲基丁醛 3-Methylbutanal C5H10O 0.86 0.10 1.06 0.70 0.20 0.93
    39 2.90 2-甲基丁醛 2-Methylbutanal C5H10O 0.19 0.02 0.07
    40 3.41 正戊醛 Pentanal C5H10O 1.32 0.39 2.65 0.80 1.50 2.00
    41 4.67 E-2-戊烯醛 E-2-pentenal C5H8O 1.40 0.45 1.43 1.02 1.66 2.28
    42 5.92 正己醛 Hexanal C6H12O 14.68 6.52 11.20 5.59 13.95 14.41
    43 7.82 E-2-己烯醛 E-2-Hexenal C6H10O 1.10 3.36 0.94 1.40 0.63 1.35
    44 9.72 Z-4-庚烯醛 Z-4-Heptenal C7H12O 0.27
    45 9.81 庚醛 Heptanal C7H14O 2.49 2.65 3.86 4.22 2.50
    46 12.32 E-2-庚烯醛 E-2-Heptenal C7H12O 3.66 2.05 2.55 2.96 2.21 3.19
    47 12.56 苯甲醛 Benzaldehyde C7H6O 1.31 1.21 0.38 1.10 0.82 0.19
    48 14.52 正辛醛 Octanal C8H16O 3.17 10.86 2.81 1.44 14.20 1.71
    49 16.64 苯乙醛 Benzeneacetaldehyde C8H8O 0.15 0.05
    50 16.70 水杨醛 Salicyl aldehyde C7H6O2 0.75
    51 17.27 2-辛烯醛 2-Octenal C8H14O 6.83 5.36 5.28 7.24 4.15 5.23
    52 19.02 E-4-壬烯醛 E-4-Nonenal C9H16O 0.17
    53 19.49 正壬醛 Nonanal C9H18O 4.21 18.23 5.15 2.25 21.39 2.99
    54 22.27 E-2-壬烯醛 E-2-Nonenal C9H16O 0.12 0.17 0.11 0.11
    55 23.79 Z-4-癸烯醛 Z-4-decenal C10H18O 1.40 2.08 0.95 1.19 1.08
    56 24.35 癸醛 Decanal C10H20O 1.21 0.86 0.88 1.35 0.67 0.30
    57 24.96 β-环柠檬醛 β-Cyclocitral C10H16O 0.06 0.12 0.11 0.20 0.08 0.24
    58 26.07 Z-柠檬醛 Z-Citral C10H16O 0.04 0.15 0.18 0.09
    59 26.99 E-2-癸烯醛 E-2-Decenal C10H18O 0.19 0.97 0.42
    60 27.43 E-柠檬醛 E-Citral C10H16O 0.15 0.38 0.10 0.72 0.27 0.17
    61 28.48 E,Z-2,4-癸二烯醛 E,Z-2,4-Decadienal C10H16O 1.77 1.70 1.40 2.05 0.99
    62 29.01 十一醛 Undecanal C11H22O 0.25
    63 29.65 E,E-2,4-癸二烯醛 E,E-2,4-Decadienal C10H16O 0.11 0.11 0.29 1.04
    64 31.57 E-2-十一烯醛 E-2-Undecenal C11H20O 0.21
    烃类 Alkane
    65 1.73 正戊烷 Pentane C5H12 0.23 0.11 0.44 0.18 0.29 0.32
    66 2.02 2-甲基戊烷 2-Methylpentane C6H14 0.04
    67 2.11 3-甲基戊烷 3-Methylpentane C6H14 0.05
    68 2.22 己烷 Hexane C6H14 0.05 0.33 0.06 0.08 0.11
    69 6.92 乙基环己烷 Ethylcyclohexane C8H16 0.02
    70 9.38 苯乙烯 Styrene C8H8 0.15 0.09 0.41 0.14 0.19 0.26
    71 9.68 壬烷 Nonane C9H20 0.19
    72 11.06 α-蒎烯 α-Pipene C10H16 0.05 0.07 0.03 0.06
    73 14.29 (+)-2-蒈烯 (+)-2-Carene C10H16 0.23 0.25 0.33 0.12 0.33
    74 15.67 β-水芹烯 β-Phellandrene C10H16 0.03 0.47 0.13 0.06 0.29
    75 32.95 正十四烷 Tetradecane C14H30 0.05 0.04 0.10 0.07 0.04 0.07
    其他 Others
    76 1.58 甲硫醇 Methanethiol CH4S 0.05 0.01 0.06 0.04 0.03 0.03
    77 1.82 2-硫代丙烷 2-Thiapropane C2H6S 0.16 0.05 0.12
    78 2.29 2-甲基呋喃 2-Methylfuran C5H6O 0.03 0.02 0.04 0.03 0.05 0.04
    79 3.47 2-乙基呋喃 2-Ethylfuran C6H8O 0.30 0.12 0.27 0.11 0.25 0.36
    80 9.82 1-硝基-3-甲基丁烷 1-nitro-3-methylbutane C5H11NO2 4.63
    81 13.61 2-正丙基噻唑 2-Propylthiazole C6H9NS 0.10
    82 13.95 2-戊基呋喃 2-Amylfuran C9H14O 3.25 3.25 4.53 3.60 2.69 3.39
    83 15.96 2-异丁基噻唑 2-Isobutylthiazole C7H11NS 4.32 5.20 17.66 15.79 1.19 5.10
    84 19.11 愈创木酚 Guaiacol C7H8O2 0.71 0.18 0.63 0.30 1.33
    85 28.98 1-硝基-2-苯乙烷 1-Nitro-2-phenylethane C8H9NO2 0.51 0.04
    86 31.62 丁香酚 Eugenol C10H12O2 0.16 0.18 0.29
    注:“-”表示未检测到。
    Note: “-” indicates compound not detected.
    下载: 导出CSV

    表  2  嫁接和非嫁接大果番茄品种挥发性物质类别及相对含量

    Table  2.   Categories and relative contents of volatiles in fruits on grafted and non-grafted large-fruit varieties of tomato plants

    类别
    Category
    相对含量/%
    博收1号 S
    Boshou No.1S
    博收1号 J
    Boshou No.1J
    辉腾 S
    Huiteng S
    辉腾 J
    Huiteng J
    辉腾2号 S
    Huiteng No.2S
    辉腾2号 J
    Huiteng No.2J
    醇类 Alcohol 21.82 19.62 15.58 11.58 12.30 25.18
    酮类 Ketone 7.93 8.27 7.15 10.27 7.28 9.94
    酯类 Ester 12.93 3.51 8.81 21.49 5.00 11.65
    醛类 Aldehyde 46.72 58.31 36.89 34.10 68.82 39.75
    烃类 Alkane 0.72 0.33 2.34 0.96 0.79 1.45
    其他 Others 8.46 9.35 27.54 20.50 4.82 10.55
    下载: 导出CSV
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出版历程
  • 收稿日期:  2020-05-08
  • 修回日期:  2020-06-19
  • 网络出版日期:  2021-01-26
  • 刊出日期:  2020-11-30

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