鄂北岗地山药病害病原菌鉴定

杨小林; 张佑宏; 王佐乾; 常向前; 吕亮; 张舒; 杨新笋

doi:10.19303/j.issn.1008-0384.2021.01.008

鄂北岗地山药病害病原菌鉴定

doi: 10.19303/j.issn.1008-0384.2021.01.008

杨小林^{1, 2,},
张佑宏^3,,
王佐乾²,
常向前²,
吕亮²,
张舒^2, ,,
杨新笋^4, ,

1.
粮食作物种质创新与遗传改良湖北省重点实验室，湖北　武汉　430064
2.
湖北省农业科学院植保土肥研究所，湖北　武汉　430064
3.
湖北省生物农药工程研究中心，湖北　武汉　430064
4.
湖北省农业科学院粮食作物研究所，湖北　武汉　430064

基金项目: 粮食作物种质创新与遗传改良湖北省重点实验室基金项目（2019lzjj06）；国家重点研发计划项目（2018YFD0301305）

详细信息

作者简介:
杨小林（1971−），女，博士，研究员，研究方向：植物病原菌致病性及病虫害防控技术（E-mail：1205600908@qq.com）

共同第一作者：张佑宏（1970−），男，副研究员，研究方向：作物病虫害绿色防控技术（E-mail：2430204775@qq.com）

通讯作者:
张舒（1964－），男，研究员，研究方向：作物病虫害研究及防控（E-mail: zs6410@163.com）

杨新笋（1967－），男，博士，研究员，研究方向：鄂薯系列新品种的创制及应用（E-mail：yangxins@163.com）

中图分类号: S 432.1
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- 被引次数: 0
出版历程
- 收稿日期: 2020-07-30
- 修回日期: 2020-12-20
- 网络出版日期: 2021-02-08
- 刊出日期: 2021-01-31

Pathogens Infecting Chinese Yams Planted on Ridges in Northern Hubei

1.
Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Wuhan, Hubei 　430064, China
2.
Institute of Plant Protection and Soil Science, Hubei Academy of Agricultural Sciences, Wuhan, Hubei 　430064, China
3.
Hubei Biological Pesticide Engineering Research Center, Wuhan, Hubei 　430064, China
4.
Institute of Food Crops, Hubei Academy of Agricultural Sciences, Wuhan, Hubei 　430064, China

摘要

摘要: 目的开展鄂北岗地山药病害病原菌的鉴定，探索引起病害的病原菌及其分类地位，为进一步研究其发生规律及制定切实有效的防治措施提供依据。方法采集鄂北岗地山药地上组织病害样本，利用植物病理学组织分离法和ITS-PCR技术，通过病原物培养形态、显微观察、ITS鉴定比对和致病性测定验证，进行山药病害病原菌的分离和鉴定。结果鄂北岗地山药病害的病原真菌系链格孢属互隔交链孢霉（Alternaria alternata），刺盘孢属果生炭疽菌（Colletotrichum fructicola）和胶孢炭疽菌（Colletotrichumn gloeosporiaides），毛色二孢属假可可毛色二孢（Lasiodiplodia pseudotheobromae）。结论以上结果揭示鄂北岗地山药有多病害发生，病原菌组成复杂。山药炭疽病及其他病害均可导致山药地上组织枯萎甚至死亡。
- 山药 /
- 病原菌 /
- 形态学 /
- ITS鉴定 /
- 刺盘孢属
Abstract: Objective Pathogens that cause serious diseases on Dioscorea batatas Decne grown on the mountain ridges in northern Hubei province were investigated to facilitate drafting effective disease prevention and control measures. Method Specimens of aboveground parts of the diseased plants were collected for pathogen isolation. The isolates were identified by colonial morphology, microstructure, and blasting sequence of ITS as well as the pathogenicity determination with tissue separation of phytopathology and ITS-PCR. Result The isolated pathogenic fungi included Alternaria alternate, Colletotrichum fructicola, C. gloeosporiaides, and Lasiodiplodia pseudotheobromae. Conclusion The pathogens causing the diseases, such as anthracnose, that can lead to withering or death of Chinese yams at the region were highly diversified and complex.
- Dioscorea batatas Decne /
- pathogen /
- morphology /
- ITS identification /
- Colletotrichum

HTML全文

图 1 山药病叶

Figure 1. Diseased leaves on vines of Chinese yam

下载: 全尺寸图片幻灯片

图 2 菌落形态

注：A. C20FG001; B. C20FG002; C. C20FG003; D. C20FG004

Figure 2. Colony morphology

Note: A: C20FG001; B: C20FG002; C: C20FG003; D: C20FG004.

下载: 全尺寸图片幻灯片

图 3 显微形态

注：A. C20FG001; B. C20FG002; C. C20FG003; D. C20FG004

Figure 3. Microstructure

Note: A: C20FG001; B: C20FG002; C: C20FG003; D: C20FG004.

下载: 全尺寸图片幻灯片

图 4 菌株基因组DNA-ITS

注：M: DL 2000 marker; 泳道1-4: 菌C20FG001～C20FG004

Figure 4. Applied fragment of pathogenic fungi by DNA-ITS

Note: M: DL 2000 marker; Lanes 1-4: fungi C20FG001-C20FG004.

下载: 全尺寸图片幻灯片

图 5 山药叶片接种菌株后的发病情况

注：A. C20FG001; B. C20FG002; C. C20FG003; D. C20FG004

Figure 5. Symptoms on leaves of Chinese yam plants after artificial fungal inoculation

Note: A: C20FG001; B: C20FG002; C: C20FG003; D: C20FG004.

下载: 全尺寸图片幻灯片

参考文献(31)

[1]	韩帅, 张河庆, 吴婕, 等. 四川省山药黑斑病病原菌的鉴定 [J]. 植物保护, 2019, 45(2):68−74. HAN S, ZHANG H Q, WU J, et al. Identification of the pathogen causing black rot of yam in Sichuan Province [J]. Plant Protection, 2019, 45(2): 68−74.(in Chinese)
[2]	廖朝晖, 朱必凤, 刘安玲, 等. 山药主要生化成分含量的测定 [J]. 韶关学院学报, 2003, 24(6):67−69. doi: 10.3969/j.issn.1007-5348.2003.06.018 LIAO Z H, ZHU B F, LIU A L, et al. Analysis of major biochemical components in Chinese yam [J]. Journal of Shaoguan University, 2003, 24(6): 67−69.(in Chinese) doi: 10.3969/j.issn.1007-5348.2003.06.018
[3]	覃维治, 黄报应, 黄开航, 等. 桂南地区淮山药炭疽病发生规律及防治技术研究 [J]. 河南农业科学, 2015, 44(7):94−98. QIN W Z, HUANG B Y, HUANG K H, et al. Occurrence regularity and control techniques of yam anthracnose in southern Guangxi [J]. Journal of Henan Agricultural Sciences, 2015, 44(7): 94−98.(in Chinese)
[4]	刘永清. 山药炭疽病病原鉴定及其药剂筛选研究 [J]. 安徽农业科学, 2005, 33(12):2327, 2339. LIU Y Q. Study on the identification and the fungicide screening of the anthracnose pathogen of Chinese yam [J]. Journal of Anhui Agricultural Sciences, 2005, 33(12): 2327, 2339.(in Chinese)
[5]	黄云, 王洪波, 李庆, 等. 山药炭疽病研究: Ⅰ炭疽病的症状及其病原鉴定 [J]. 西南农业大学学报, 2004, 26(1):44−46, 54. HUANG Y, WANG H B, LI Q, et al. Studies on yam anthracnose - symptoms of yam anthracnose and identification of its pathogen [J]. Journal of Southwest Agricultural University, 2004, 26(1): 44−46, 54.(in Chinese)
[6]	朱桂宁, 蔡健和, 胡春锦, 等. 广西山药炭疽病病原菌的鉴定与ITS序列分析 [J]. 植物病理学报, 2007, 37(6):572−577. doi: 10.3321/j.issn:0412-0914.2007.06.003 ZHU G N, CAI J H, HU C J, et al. Identification and ITS sequence analysis of the pathogen of yam anthracnose in Guangxi [J]. Acta Phytopathologica Sinica, 2007, 37(6): 572−577.(in Chinese) doi: 10.3321/j.issn:0412-0914.2007.06.003
[7]	傅俊范, 王思文, 周如军, 等. 辽宁山药炭疽病病原鉴定及其生物学特性 [J]. 吉林农业大学学报, 2014, 36(4):395−400. FU J F, WANG S W, ZHOU R J, et al. Pathogen identification of yam anthracnose and its biological characteristics in Liaoning Province [J]. Journal of Jilin Agricultural University, 2014, 36(4): 395−400.(in Chinese)
[8]	《英汉植物病理学词汇》编辑委员会. 英汉植物病理学词汇[M]. 第2版. 北京: 中国农业出版社, 2001.
[9]	林永康. 福建沙县山药炭疽病病原鉴定及其防治 [J]. 亚热带植物通讯, 1998(2):30−33. LIN Y K. Identification and control of the pathogen of Chinese yam anthracnose in Shaxian, Fujian [J]. Subtropical Plant Science, 1998(2): 30−33.(in Chinese)
[10]	DEAN R, VAN KAN J A L, PRETORIUS Z A, et al. The Top 10 fungal pathogens in molecular plant pathology [J]. Molecular Plant Pathology, 2012, 13(4): 414−430. doi: 10.1111/j.1364-3703.2011.00783.x
[11]	SREENIVASAPRASAD S, BROWN A E, MILLS P R. DNA sequence variation and interrelationships among Colletotrichum species causing strawberry anthracnose [J]. Physiological and Molecular Plant Pathology, 1992, 41(4): 265−281. doi: 10.1016/0885-5765(92)90026-R
[12]	HYDE K D, CAI L, CANNON P F, et al. Colletotrichum-names in current use [J]. Fungal Diversity, 2009, 39: 147−182.
[13]	FU M, CROUS P W, BAI Q, et al. Colletotrichum species associated with anthracnose of Pyrus spp. in China [J]. Persoonia - Molecular Phylogeny and Evolution of Fungi, 2019, 42(1): 1−35. doi: 10.3767/persoonia.2019.42.01
[14]	方中达. 植病研究方法[M]. 第 3版. 北京: 中国农业出版社, 1998.
[15]	刘娟. 柑桔园链格孢菌的鉴定与防治[D]. 重庆: 西南大学, 2011. LIU J. Identification and control of Alternaria sp. in citrus orchards[D]. Chongqing: Southwest University, 2011. (in Chinese).
[16]	杨小军, 倪汉文, 杨立军, 等. 小麦叶片上小麦白粉病菌的三种染色方法比较 [J]. 华中农业大学学报, 2004, 33:39−40. doi: 10.3321/j.issn:1000-2421.2004.z1.009 YANG X J, NI H W, YANG L J, et al. Comparison of three dyeing methods of wheat powdery mildew on wheat leaves [J]. Journal of Huazhong Agricultural University, 2004, 33: 39−40.(in Chinese) doi: 10.3321/j.issn:1000-2421.2004.z1.009
[17]	何月秋. 真菌菌丝体培养和提取DNA方法的改进 [J]. 菌物系统, 2000, 19(3):434. doi: 10.3969/j.issn.1672-6472.2000.03.030 HE Y Q. An improved protocol for fungal DNA preparation [J]. Mycosystema, 2000, 19(3): 434.(in Chinese) doi: 10.3969/j.issn.1672-6472.2000.03.030
[18]	张永信, 刘晓杰, 冯争光, 等. 金银花褐斑病病原检测和ITS鉴定 [J]. 河北农业大学学报, 2016, 39(4):73−77. ZHANG Y X, LIU X J, FENG Z G, et al. Pathogen detection and ITS identification of Lonicera japonica brown spot [J]. Journal of Agricultural University of Hebei, 2016, 39(4): 73−77.(in Chinese)
[19]	王佳宁, 赵洪海, 尉莹莹, 等. 蓝莓毛色二胞枝枯病的病原菌 [J]. 菌物学报, 2016, 35(6):657−665. WANG J N, ZHAO H H, YU Y Y, et al. The pathogen causing Lasiodiplodia twig blight of blueberry [J]. Mycosystema, 2016, 35(6): 657−665.(in Chinese)
[20]	李红叶, 梅秀凤, 符雨诗, 等. 柑橘链格孢褐斑病的发生危害风险和治理对策 [J]. 果树学报, 2015, 32(5):969−976. LI H Y, MEI X F, FU Y S, et al. Alternaria brown spot of citrus: The risk and management strategy [J]. Journal of Fruit Science, 2015, 32(5): 969−976.(in Chinese)
[21]	葛慈斌, 黄素芳, 阮传清, 等. 建阳桔柚两种真菌病原菌的鉴定 [J]. 福建农业学报, 2019, 34(8):958−964. GE C B, HUANG S F, RUAN C Q, et al. Pathogens of diseases on Jianyang tangelos [J]. Fujian Journal of Agricultural Sciences, 2019, 34(8): 958−964.(in Chinese)
[22]	何丽. 新疆枣果黑斑病菌交链格孢菌产生毒素种类及其致病作用研究[D]. 石河子: 石河子大学, 2017. HE L. Mycotoxins analysis to Alternaria alternata that mainly pathogen of ujujbe black spot and toxins caused disease effect to ujujbe in Xinjiang[D]. Shihezi : Shihezi University, 2017. (in Chinese).
[23]	WEIR B S, JOHNSTON P R, DAMM U. The Colletotrichum gloeosporioides species complex [J]. Studies in Mycology, 2012, 73: 115−180. doi: 10.3114/sim0011
[24]	LIU F, DAMM U, CAI L, et al. Species of the Colletotrichum gloeosporioides complex associated with anthracnose diseases of Proteaceae [J]. Fungal Diversity, 2013, 61(1): 89−105. doi: 10.1007/s13225-013-0249-2
[25]	谢昀烨, 曾思锦, 袁月, 等. 浙江省新昌县茶叶炭疽病病原鉴定及其药剂敏感性测定 [J]. 浙江农业学报, 2018, 30(7):1188−1193. doi: 10.3969/j.issn.1004-1524.2018.07.12 XIE Y Y, ZENG S J, YUAN Y, et al. Pathogen identification and susceptibility to fungicides on tea anthracnose in Xinchang of Zhejiang Province [J]. Acta Agriculturae Zhejiangensis, 2018, 30(7): 1188−1193.(in Chinese) doi: 10.3969/j.issn.1004-1524.2018.07.12
[26]	宋慧云, 段志豪, 韦嘉怡, 等. 多穗柯炭疽病病原鉴定及其不同杀菌剂的室内毒力测定 [J]. 南方农业学报, 2019, 50(3):593−599. SONG H Y, DUAN Z H, WEI J Y, et al. Pathogen identification of Lithocarpus polystachyus Rehd. anthracnose and indoor toxicity measurement of different fungicides [J]. Journal of Southern Agriculture, 2019, 50(3): 593−599.(in Chinese)
[27]	王妮, 尹显慧, 彭丽娟, 等. 辣椒炭疽病病原鉴定及其杀菌剂毒力测定 [J]. 植物保护, 2019, 45(4):216−223. WANG N, YIN X H, PENG L J, et al. Identification of the pathogen and toxicity test of fungicides to capsicum anthracnos [J]. Plant Protection, 2019, 45(4): 216−223.(in Chinese)
[28]	李海云, 靳帅, 张学勤, 等. 大豆炭疽病菌Colletotrichum chlorophyti的鉴定 [J]. 植物保护, 2017, 43(2):163−166. doi: 10.3969/j.issn.0529-1542.2017.02.028 LI H Y, JIN S, ZHANG X Q, et al. Identification of Colletotrichum chlorophyti causing soybean anthracnose [J]. Plant Protection, 2017, 43(2): 163−166.(in Chinese) doi: 10.3969/j.issn.0529-1542.2017.02.028
[29]	王步云, 乔岩, 张涛, 等. 北京地区草莓炭疽病病原鉴定及生物防治药剂筛选 [J]. 河南农业科学, 2019, 48(4):88−92. WANG B Y, QIAO Y, ZHANG T, et al. Identification of pathogens causing strawberry anthracnose and screening of biopesticides in Beijing area [J]. Journal of Henan Agricultural Sciences, 2019, 48(4): 88−92.(in Chinese)
[30]	顾红杰, 郭利军, 赵亚, 等. 菠萝蜜炭疽病病原菌的形态学与多基因分子系统学鉴定 [J]. 分子植物育种, 2019, 17(5):1487−1492. GU H J, GUO L J, ZHAO Y, et al. Identification of pathogen causing jackfruit anthracnose based on morphology and multi-gene sequence analysis [J]. Molecular Plant Breeding, 2019, 17(5): 1487−1492.(in Chinese)
[31]	王薇. 苹果炭疽叶枯病病原学及苹果炭疽病侵染来源研究[D]. 杨凌: 西北农林科技大学, 2017. WANG W. Etiology of apple glomerallaleaf spot caused by Colletotrichum and infection sources of apple bitter bot[D]. Yangling: Northwest A & F University, 2017. (in Chinese).