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喷雾干燥法制备玫瑰茄花色苷微胶囊条件优化

赵彦巧 王月 孟翔宇 李钰琨

赵彦巧,王月,孟翔宇,等. 喷雾干燥法制备玫瑰茄花色苷微胶囊条件优化 [J]. 福建农业学报,2021,36(1):104−114 doi: 10.19303/j.issn.1008-0384.2021.01.013
引用本文: 赵彦巧,王月,孟翔宇,等. 喷雾干燥法制备玫瑰茄花色苷微胶囊条件优化 [J]. 福建农业学报,2021,36(1):104−114 doi: 10.19303/j.issn.1008-0384.2021.01.013
ZHAO Y Q, WANG Y, MENG X Y, et al. Optimized Preparation of Spray-dried Anthocyanins Microcapsules [J]. Fujian Journal of Agricultural Sciences,2021,36(1):104−114 doi: 10.19303/j.issn.1008-0384.2021.01.013
Citation: ZHAO Y Q, WANG Y, MENG X Y, et al. Optimized Preparation of Spray-dried Anthocyanins Microcapsules [J]. Fujian Journal of Agricultural Sciences,2021,36(1):104−114 doi: 10.19303/j.issn.1008-0384.2021.01.013

喷雾干燥法制备玫瑰茄花色苷微胶囊条件优化

doi: 10.19303/j.issn.1008-0384.2021.01.013
基金项目: 天津市科技支撑计划重点项目(19YFZCSN00010);天津商业大学“国家级大学生创新创业训练计划”项目(201810069002); 天津商业大学“市级大学生创新创业训练计划”项目(202010069048)
详细信息
    作者简介:

    赵彦巧(1979−),女,博士,副教授,研究方向:天然产物的提取与分离(E-mail:yqzhao@tjcu.edu.cn

    通讯作者:

    赵彦巧(1979−),女,博士,副教授,研究方向:天然产物的提取与分离(E-mail:yqzhao@tjcu.edu.cn

  • 中图分类号: TS 201

Optimized Preparation of Spray-dried Anthocyanins Microcapsules

  • 摘要:   目的  优化玫瑰茄花色苷微胶囊喷雾干燥工艺,提高玫瑰茄花色苷的包埋率及稳定性。  方法  以阿拉伯树胶和麦芽糊精为壁材,玫瑰茄花色苷为芯材,采用单因素试验和响应面法优化玫瑰茄花色苷微胶囊制备工艺条件,用扫描电镜观察花色苷微胶囊的微观形貌,考察其对光、热及金属的稳定性。  结果  玫瑰茄花色苷微胶囊制备的最佳工艺参数为:麦芽糊精占总固形物质量分数70.0%,阿拉伯树胶占总固形物质量分数3.0%,玫瑰茄花色苷占总固形物质量分数7.0%,总固形物质量分数7.0%,喷雾干燥进风温度143℃,此条件下玫瑰茄花色苷的包埋率为97.11%。扫描电镜结果显示微胶囊呈圆球形,表面较光滑致密,无裂痕无孔洞。微胶囊玫瑰茄花色苷稳定性比较分析表明:在相同温度、相同光照条件下,玫瑰茄花色苷微胶囊稳定性均明显高于纯化物;微胶囊化后花色苷在Cu2+和Zn2+中的稳定性得到明显改善。且玫瑰茄花色苷微胶囊在4℃、避光以及避免与Cu2+和Zn2+接触的条件下稳定性最高。  结论  通过优化玫瑰茄花色苷微胶囊制备工艺,可有效提高玫瑰茄花色苷的稳定性。
  • 图  1  阿拉伯树胶占总固形物质量分数对微胶囊包埋率的影响

    Figure  1.  Effect of mass fraction of gum Arabic in total solids on encapsulation

    图  2  花色苷占总固形物质量分数对微胶囊包埋率的影响

    Figure  2.  Effect of mass fraction of anthocyanin in total solids on encapsulation

    图  3  总固形物质量分数对微胶囊包埋率的影响

    Figure  3.  Effect of total solid content on encapsulation

    图  4  进风温度对微胶囊包埋率的影响

    Figure  4.  Effect of spray-drying inlet-air temperature on encapsulation

    图  5  各因素交互作用对微胶囊包埋率影响的响应面图

    Figure  5.  Response surface contours showing interactions of various factors in microencapsulation

    图  6  玫瑰茄花色苷微胶囊的SEM图

    Figure  6.  SEM micrograph of H. sabdariffa anthocyanin microcapsule

    图  7  温度对微胶囊花色苷稳定性的影响

    Figure  7.  Effect of temperature on stability of anthocyanin microcapsules

    图  8  光照对微胶囊花色苷稳定性的影响

    Figure  8.  Effect of light on stability of anthocyanin microcapsules

    图  9  金属离子对微胶囊花色苷稳定性的影响

    Figure  9.  Effect of metal ions on stability of anthocyanin microcapsules

    表  1  响应面试验因素与水平

    Table  1.   Factors and levels of response surface design

    水平
    Level
    因素 Factor
    A阿拉伯胶占
    总固形物
    质量分数
    Mass fraction of
    gum arabic
    in total solid/%
    B花色苷占
    总固形物
    质量分数
    Mass fraction of
    anthocyaninintotal
    solid/%
    C 总固形物
    质量分数
    Total solid
    mass fraction/
    %
    D进风温度
    Inlet-air
    temperature/℃
    −1 2 3 7 140
    0 3 5 9 160
    1 4 7 11 180
    下载: 导出CSV

    表  2  响应面试验结果

    Table  2.   Results of response surface test

    序号
    No.
    A阿拉伯树胶
    占总固形物
    质量分数
    Mass fraction
    of gum
    arabic in
    total solid/%
    B花色苷占
    总固形物
    质量分数
    Mass fraction
    of anthocyanin
    in total
    solid/%
    C总固形
    物质量
    分数
    Total
    solid
    mass
    fraction/%
    D进风
    温度
    Inlet-air
    temperature/
    Y包埋率
    Embedding
    rate/%
    1 −1 −1 0 0 94.20
    2 −1 0 0 1 95.14
    3 1 0 1 0 95.84
    4 0 0 1 −1 93.51
    5 −1 1 0 0 96.26
    6 −1 0 −1 0 95.34
    7 0 0 0 0 96.73
    8 0 0 0 0 96.89
    9 1 1 0 0 96.29
    10 0 1 0 −1 97.25
    11 0 −1 −1 0 96.45
    12 0 −1 0 1 95.44
    13 0 0 0 0 97.35
    14 −1 0 1 0 95.17
    15 0 0 0 0 96.30
    16 0 0 −1 1 95.44
    17 0 1 0 1 96.26
    18 0 0 0 0 97.12
    19 0 0 1 1 96.41
    20 1 0 −1 0 96.84
    21 0 −1 1 0 94.29
    22 1 −1 0 0 95.90
    23 1 0 0 −1 94.72
    24 −1 0 0 −1 95.54
    25 0 1 1 0 96.23
    26 0 1 −1 0 97.25
    27 0 −1 0 −1 94.62
    28 1 0 0 1 96.88
    29 0 0 −1 −1 96.37
    下载: 导出CSV

    表  3  回归模型方差分析

    Table  3.   Analysis of variance on regression model

    方差来源
    Sources of variance
    平方和
    Sum of squares
    自由度
    df
    均方
    Mean square
    F
    F-value
    P
    P-value
    显著性
    Significance
    模型 <0.0001 <0.0001 1.84 9.87 <0.0001 **
    A阿拉伯树胶占总固形物质量分数 0.0061 0.0061 1.94 10.38 0.0061 **
    B花色苷占总固形物质量分数 <0.0001 <0.0001 6.22 33.35 <0.0001 **
    C固形物质量分数 0.0009 0.0009 3.24 17.40 0.0009 **
    D进风温度 0.0321 0.0321 1.06 5.66 0.0321 *
    AB 0.0737 0.0737 0.70 3.74 0.0737
    AC 0.3529 0.3529 0.17 0.92 0.3529
    AD 0.0103 0.0103 1.64 8.78 0.0103 *
    BC 0.2081 0.2081 0.32 1.74 0.2081
    BD 0.0548 0.0548 0.82 4.39 0.0548
    CD 0.0006 0.0006 3.67 19.66 0.0006 **
    A2 0.0016 0.0016 2.82 15.10 0.0016 **
    B2 0.0472 0.0472 0.88 4.74 0.0472 *
    C2 0.0073 0.0073 1.83 9.82 0.0073 **
    D2 0.0008 0.0008 3.42 18.36 0.0008 **
    残差 2.61 14 0.19
    失拟项 1.97 10 0.20 1.24 0.4521
    净误差 0.64 4 0.16
    总离差 28.39 28
    决定系数R2=0.9080 校正系数0.8446
    下载: 导出CSV

    表  4  不同样品理化指标测定结果

    Table  4.   Physiochemical properties of samples

    样品
    Sample
    理化指标 Physical and chemical indexes
    水分含量
    Moisture content/%
    堆密度
    Heap density/(g·mL−1)
    花色苷纯化物10.42±0.820.52
    花色苷微胶囊3.49±0.070.89
    下载: 导出CSV
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  • 收稿日期:  2020-07-18
  • 修回日期:  2020-10-06
  • 刊出日期:  2021-01-31

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