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不同荫蔽度对香露兜光合特征及香气成分的影响

唐瑾暄 鱼欢 郭彩权 秦晓威 白亭玉 宗迎

唐瑾暄,鱼欢,郭彩权,等. 不同荫蔽度对香露兜光合特征及香气成分的影响 [J]. 福建农业学报,2020,35(8):820−829 doi: 10.19303/j.issn.1008-0384.2020.08.002
引用本文: 唐瑾暄,鱼欢,郭彩权,等. 不同荫蔽度对香露兜光合特征及香气成分的影响 [J]. 福建农业学报,2020,35(8):820−829 doi: 10.19303/j.issn.1008-0384.2020.08.002
TANG J X, YU H, GUO C Q, et al. Effects of Shading on Photosynthesis and Aromatics of Pandan ( Pandanus amaryllifolius) Plants [J]. Fujian Journal of Agricultural Sciences,2020,35(8):820−829 doi: 10.19303/j.issn.1008-0384.2020.08.002
Citation: TANG J X, YU H, GUO C Q, et al. Effects of Shading on Photosynthesis and Aromatics of Pandan ( Pandanus amaryllifolius ) Plants [J]. Fujian Journal of Agricultural Sciences,2020,35(8):820−829 doi: 10.19303/j.issn.1008-0384.2020.08.002

不同荫蔽度对香露兜光合特征及香气成分的影响

doi: 10.19303/j.issn.1008-0384.2020.08.002
基金项目: 海南省自然科学基金项目(319MS085);海南省基础与应用基础研究计划(自然科学领域)高层次人才项目(2019RC323)
详细信息
    作者简介:

    唐瑾暄(1996−),女,硕士研究生,主要从事高效栽培机理研究(E-mail:787145063@qq.com

    通讯作者:

    秦晓威(1982−),男,博士,副研究员,主要从事种质资源收集保存、鉴定评价与利用研究(E-mail:qin_xiaowei@163.com

  • 中图分类号: S 573

Effects of Shading on Photosynthesis and Aromatics of Pandan (Pandanus amaryllifolius) Plants

  • 摘要:   目的  研究不同荫蔽度对香露兜光合特性、生长情况及香气成分的影响,为生产上林下复合种植香露兜提供理论依据。  方法  采用人工荫蔽盆栽试验的方法,设置全光照、30%荫蔽度、60%荫蔽度和90%荫蔽度等4种不同荫蔽处理,探究不同荫蔽度处理下香露兜光合参数、生长指标和香气成分对光环境的响应。  结果  荫蔽度为30%和60%时,香露兜净光合速率和气孔导度均显著高于全光照和90%遮荫处理,有利于提高香露兜的光合作用,植株叶片较多。随着荫蔽度的增加,分蘖数显著减少。4种遮荫处理下香露兜共鉴定出27种挥发性物质,2-乙酰-1-吡咯啉、叶绿醇、角鲨烯、丙醇、丙酮醇、3-甲基-2-(5H)-呋喃酮、新植二烯、棕榈酸乙酯、2,3-二氢苯并呋喃和亚油酸乙酯等10种共有香气成分含量差异显著。30%和60%荫蔽处理下香露兜叶片关键特征香气物质2-乙酰-1-吡咯啉和3-甲基-2-(5H)-呋喃酮、2,3-二氢苯并呋喃、亚油酸乙酯和叶绿醇显著高于其他处理,30%荫蔽处理角鲨烯和叶绿醇含量显著高于其他处理,60%荫蔽处理丙酮醇、2-乙酰-1-吡咯啉、3-甲基-2-(5H)-呋喃酮、新植二烯、棕榈酸乙酯和亚油酸乙酯显著高于其他处理。  结论  30%~60%荫蔽度可促进香露兜生长,提高主要香气成分含量,风味品质佳。
  • 图  1  遮荫处理下香露兜叶片的光合参数

    注:图中不同小写字母表示4种遮荫处理间光合参数的差异显著性水平(P<0.05)。

    Figure  1.  Photosynthetic indices on leaves of pandan plants grown under varied shading treatments

    Note: Data with different lowercase letters indicate significant difference on photosynthetic parameters among treatments at 0.05 level.

    图  2  不同荫蔽度下香露兜的叶片数(A)和分蘖数(B)

    注:图中不同小写字母表示4种遮荫处间叶片生长状况的差异显著性水平(P<0.05)。

    Figure  2.  Number of leaves (A) and tillers (B) on pandan plant grown under varied shading treatments

    Note: Data with different lowercase letters indicate significant difference on growth of leaves among treatments at 0.05 level.

    图  3  遮荫处理下香露兜叶片香气成分种类数量(A)和含量(B)

    注:图中不同小写字母表示4种遮荫处理间同种香气成分化合物种类的差异显著性水平(P<0.05);Ⅰ-吡咯类,Ⅱ-醇类,Ⅲ-呋喃类,Ⅳ-酚类,Ⅴ-呋喃酮类,Ⅵ-烃类,Ⅶ-酮类,Ⅷ-酯类.

    Figure  3.  Categories (A) and contents (B) of volatile compounds in leaves of pandan plant grown under varied shading treatments

    Note: Data with different lowercase letters indicate significant difference on same aromatic compound among treatments at 0.05 level; Ⅰ-Pyrroles,Ⅱ-Alcohols,Ⅲ-Furans,Ⅳ-Phenols,Ⅴ-Furanones,Ⅵ-Hydrocarbons,Ⅶ-Ketones,Ⅷ-Esters.

    图  4  不同荫蔽度香露兜叶片香气成分PCA分析

    注:A.香气成分组成主成分分析;B.香气成分种类主成分分析;代码为香气成分(表1);黑色圆点表示香露兜27种香气成分,黑色三角表示香露兜8种香气成分种类。

    Figure  4.  Biplot of principal components analysis showing aromatics in leaves of pandan plants grown under varied shading treatments

    Note: A: on aromatic compounds; B: on categories of aromatics; sample codes: for aromatic components (Table 1); black dots: 27 aromatic compounds; black triangles: 8 classes of aromatics in pandan plants.

    表  1  遮荫处理下香露兜叶片挥发性香气成分及含量

    Table  1.   Volatiles in leaves of pandan plant grown under varied shading treatments

    化合物种类
    classes
    保留时间
    RT
    保留指数
    LRI
    化合物名称
    compounds
    代码
    code
    遮荫度 The shade degree/%
    0306090
    脂类 Esters 9.67 1217 丙酮酸甲酯 methyl pyruvate H1 12.58±0.37 8.23±0.45
    12.07 1349 L-乳酸乙酯(−)-Ethyl L-lactate H2 31.07±1.04 11.47±0.33 4.88±0.12
    14.24 1436 乙醇酸乙酯 Ethyl 2-hydroxyacetate H3 8.36±0.22 2.30±0.09 2.92±0.05
    15.21 1474 (S)-缩水甘油乙酸酯
    oxiran-2-ylmethyl acetate
    H4 1.33±0.02
    29.93 2142 (±)-α-羟基-γ-丁内酯 α-butyrolactone H5 24.76±0.11 19.82±0.25 33.56±0.15
    31.65 2251 棕榈酸乙酯 ethyl palmitate H6 21.73±0.19 1.55±0.05 34.44±0.70 14.00±0.27
    34.86 2471 油酸乙酯 ethyl oleate H7 5.30±0.06 7.24±0.29 3.32±0.19
    35.49 2521 亚油酸乙酯 Ethyl linoleate H8 12.73±0.78 25.71±0.51 37.54±1.62 19.78±0.48
    36.59 2591 亚麻酸乙酯 ethyl linolenate H9 22.91±0.96
    醇类 Alcohols 7.62 1036 丙醇 1-propanol B1 4.02±0.04 3.78±0.05 3.77±0.16 4.29±0.15
    9.44 1209 异戊醇 isoamyl alcohol B2 2.27±0.08 1.16±0.10
    20.73 1658 1,2-乙二醇 1,2-ethanediol B3 13.95±0.68
    36.82 2622 叶绿醇 Phytol B4 62.40±1.28 149.23±0.64 124.37±0.59 70.63±0.96
    烃类 Hydrocarbons
    13.14 1400 正十四烷 tetradecane F1 3.12±0.01
    19.08 1600 正十六烷 hexadecane F2 6.70±1.15 3.13±0.21
    23.66 1800 十八烷 octadecane F3 1.33±0.01
    25.99 1922 新植二烯 neophytadiene F4 16.05±0.21 26.36±0.26 54.67±0.87 36.66±0.18
    39.85 2865 角鲨烯 Squalene F5 609.38±4.09 784.72±6.87 413.47±17.74 41.64±1.5
    酮类 Ketones 10.83 1284 3-羟基-2-丁酮 acetoin G1 9.38±0.51 5.11±0.03 9.02±0.18
    11.23 1303 丙酮醇 acetol G2 95.15±5.99 87.36±0.51 155.76±0.9 20.01±0.19
    20.80 1668 1,2-环己二酮 Cyclohexan-1,2-dion G3 4.37±0.09
    24.25 1830 甲基环戊烯醇酮 cyclotene G4 3.21±0.64
    25.49 1894 3-甲基环戊烷-1,2-二酮
    3-ethyl-1,2-cyclopentanedione
    G5 3.16±0.06
    吡咯类 Pyrroles 11.79 1330 2-乙酰基-1-吡咯啉 2-acetyl-1-pyrrolidine A1 5.41±0.08 11.76±0.21 13.88±1.64 4.48±0.14
    酚类 Phenols 32.62 2318 2,4-二叔丁基苯酚 2,4-di-tert-butylphenol C1 12.97±0.12 15.16±0.43
    呋喃 Furans 33.66 2389 2,3-二氢苯并呋喃 coumaran D1 61.94±5.80 86.00±7.22 94.92±14.73 19.82±1.13
    呋喃酮 Furanone 21.64 1713 3-甲基-2-(5H)-呋喃酮
    3-methyl-2(5H)-furanone
    E1 32.04±0.44 43.92±1.03 55.46±0.17 16.27±0.11
    注:“―”表示未检测到该物质;数据后不同小写字母表示4种遮荫处理间香气成分含量的差异显著性水平(P<0.05)。
    Note: “―” indicates substance not detected; data with different lowercase letters indicate significant difference on aromatic contents among treatments at 0.05 level.
    下载: 导出CSV
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  • 收稿日期:  2020-04-17
  • 修回日期:  2020-06-11
  • 刊出日期:  2020-08-19

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