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盐胁迫下异甜菊醇浸种对油菜种子萌发和幼苗生长的影响

张亮 李玉婷 夏文静 许晓风

张亮,李玉婷,夏文静,等. 盐胁迫下异甜菊醇浸种对油菜种子萌发和幼苗生长的影响 [J]. 福建农业学报,2020,35(8):883−890 doi: 10.19303/j.issn.1008-0384.2020.08.011
引用本文: 张亮,李玉婷,夏文静,等. 盐胁迫下异甜菊醇浸种对油菜种子萌发和幼苗生长的影响 [J]. 福建农业学报,2020,35(8):883−890 doi: 10.19303/j.issn.1008-0384.2020.08.011
ZHANG L, LI Y T, XIA W J, et al. Effect of Pre-soaking Seeds in Isosteviol Solution on Germination and Seedling Growth of Brassica napus under Salt Stress [J]. Fujian Journal of Agricultural Sciences,2020,35(8):883−890 doi: 10.19303/j.issn.1008-0384.2020.08.011
Citation: ZHANG L, LI Y T, XIA W J, et al. Effect of Pre-soaking Seeds in Isosteviol Solution on Germination and Seedling Growth of Brassica napus under Salt Stress [J]. Fujian Journal of Agricultural Sciences,2020,35(8):883−890 doi: 10.19303/j.issn.1008-0384.2020.08.011

盐胁迫下异甜菊醇浸种对油菜种子萌发和幼苗生长的影响

doi: 10.19303/j.issn.1008-0384.2020.08.011
基金项目: 江苏省高等学校自然科学研究面上项目(2020);江苏省高校实验室研究会立项课题(GS2019YB06)
详细信息
    作者简介:

    张亮(1987−),男,硕士,讲师,研究方向:植物逆境生理与植物营养(E-mail:liangzai0061@126.com

    通讯作者:

    许晓风(1959−),男,博士,教授,研究方向:植物生理与分子生物学(E-mail:xuxiaofeng@njnu.edu.cn

  • 中图分类号: Q 945.78

Effect of Pre-soaking Seeds in Isosteviol Solution on Germination and Seedling Growth of Brassica napus under Salt Stress

  • 摘要:   目的  探索异甜菊醇浸种对盐胁迫下油菜种子发芽及幼苗生长的影响。  方法  以秦油2号油菜种子为材料,在盐胁迫下添加不同浓度的异甜菊醇,对其进行浸种处理。统计油菜种子的发芽率、发芽指数和活力指数,测定幼苗苗高、根长、叶绿素含量、根系活力、丙二醛(MDA)含量及抗氧化酶活性等生理指标。  结果  单纯盐胁迫下,油菜种子的发芽力受到抑制,种子发芽指标显著减低,NaCl敏感浓度为140 mmol·L−1;1×10−8 mol·L−1异甜菊醇浸种处理可显著增加盐胁迫下油菜幼苗的根系活力、子叶中叶绿素总量和SOD活性,分别比CK2(去离子水浸种、140 mmol·L−1NaCl胁迫)增加27.33%、36.94%和83.31%;显著降低幼苗子叶的MDA的含量,比CK2降低32.11%。而1×10−9 mol·L−1异甜菊醇浸种处理可显著增加油菜幼苗子叶的POD和CAT活性,分别比CK2增加140.80%和47.25%。异甜菊醇浸种还可显著提高油菜种子的发芽率、发芽指数和活力指数,显著增加幼苗的苗高和根长。  结论  用适宜浓度(1×10−9~1×10−8 mol·L−1)的异甜菊醇浸种可有效增强油菜幼苗的抗盐能力,缓解盐胁迫对种子发芽和幼苗生长造成的伤害。
  • 图  1  NaCl胁迫下异甜菊醇浸种对油菜根系活力的影响

    注:不同字母表示不同处理间存在显著差异(P<0.05),下同。

    Figure  1.  Effect of pre-soaking seeds in isosteviol solution on root activity of B. napus seedlings under salt stress

    Note: Data with different letter indicate significant difference between different treatments at P <0.05. Same for the following unless stated otherwise.

    图  2  NaCl胁迫下异甜菊醇浸种对油菜幼苗(鲜样)叶绿素含量的影响

    Figure  2.  Effect of pre-soaking seeds in isosteviol solution on chlorophyll content of B. napus seedlings (fresh samples) under salt stress

    图  3  NaCl胁迫下异甜菊醇浸种对油菜幼苗子叶(鲜样)丙二醛含量的影响

    Figure  3.  Effect of pre-soaking seeds in isosteviol solution on MDA content of B. napus seedlings (fresh samples) under salt stress

    图  4  NaCl胁迫下异甜菊醇浸种对油菜幼苗子叶(鲜样)抗氧化酶活性的影响

    Figure  4.  Effects of pre-soaking seeds in isosteviol solution on SOD, POD, CAT activities of B. napus seedlings (fresh samples) under salt stress

    表  1  NaCl胁迫对油菜种子发芽指标的影响

    Table  1.   Effect of salt stress on seed germination of B. napus

    NaCl 浓度
    NaCl concentration/(mmol·L−1
    发芽率
    Germination rate/%
    发芽指数
    Germination index
    活力指数
    Vitality index
    苗高
    Stem length/cm
    根长
    Root length/cm
    0(CK193.00±1.51 Aa43.71±0.92 Aa576.97±5.80 Aa3.40±0.28 Aa9.80±1.41 Aa
    4091.30±2.62 Aab41.02±1.41 Aab500.44±3.30 Bb3.50±0.32 Aa8.70±1.12 Bb
    8090.70±1.71 ABb37.61±1.31 Bb304.64±4.60 Cc2.70±0.56 Bb5.40±0.71 Cc
    10090.20±1.12 ABb36.82±1.72 Bbc217.24±2.30 Dd2.30±0.73 Cbc3.60±0.42 Dd
    12085.10±0.91 Bc32.41±1.91 Cc184.74±1.91 Ee2.20±1.11 Cc3.50±0.41 Dd
    14073.60±1.61 Cd26.53±0.72 Dd124.69±1.80 Ff1.80±0.82 Dd2.90±0.11 Ee
    16049.50±1.72 De12.26±0.41 Ee31.88±2.30 Gg1.20±1.38 Ee1.40±0.11 Ff
    注:同一列无相同大写字母表示处理间差异极显著(P<0.01);同一列无相同小写字母表示处理间差异显著(P<0.05);CK1为去离子水浸种和发芽处理组。
    Note: Data with different capitalized letters on same column indicate significant difference between treatments at P<0.01; those with same lowercase letters on same column indicate significant difference between treatments at P<0.05; CK1 indicates control group by soaking and germinating seeds in deionized water.
    下载: 导出CSV

    表  2  NaCl胁迫下异甜菊醇浸种对油菜种子发芽及幼苗生长的影响

    Table  2.   Effects of pre-soaking in isosteviol solution on seed germination and seedling growth of B. napus under salt stress

    异甜菊醇浓度Isosteviol concentration/
    (mol·L−1
    发芽率
    Germination rate/%
    发芽指数
    Germination index
    活力指数
    Vitality index
    苗高
    Stem length/cm
    根长
    Root length/cm
    0(CK192.50±1.52 a42.82±0.90 a560.94±5.82 a3.50±0.28 a9.60±1.40 a
    0(CK274.70±2.61 e29.13±0.81 d136.91±1.72 f1.70±0.31 e3.00±0.10 f
    1×10−1079.31±1.42 cd34.13±1.12 c211.61±0.92 e2.20±0.51 d4.00±0.11 e
    1×10−985.22±1.20 b37.04±1.93 b288.91±1.71 c2.90±0.20 b4.90±0.22 c
    1×10−884.11±2.10 b38.75±1.01 b337.13±2.83 b2.90±0.41 b5.80±0.23 b
    1×10−781.63±1.61 c36.91±1.04 bc265.75±2.74 cd2.60±0.51 c4.60±0.41 cd
    1×10−677.52±2.02 d35.92±1.53 c255.03±1.73 d2.60±0.42 c4.50±0.32 d
    注:CK1为去离子水浸种、发苗处理组;CK2为去离子水浸种、140 mmol·L−1 NaCl胁迫发苗处理组,下同。
    Note: CK1 indicates control group by soaking and germinating seeds in deionized water. CK2 indicates control group by pre-soaking seeds in deionized water and germinating in medium containing NaCl 140 mmol·L−1. Same for the following unless stated otherwise.
    下载: 导出CSV
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