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福建省七叶一枝花细菌性软腐病病原鉴定

郑梅霞 苏海兰 肖荣凤 何桂强 朱育菁

郑梅霞,苏海兰,肖荣凤,等. 福建省七叶一枝花细菌性软腐病病原鉴定 [J]. 福建农业学报,2021,36(3):332−336 doi: 10.19303/j.issn.1008-0384.2021.03.012
引用本文: 郑梅霞,苏海兰,肖荣凤,等. 福建省七叶一枝花细菌性软腐病病原鉴定 [J]. 福建农业学报,2021,36(3):332−336 doi: 10.19303/j.issn.1008-0384.2021.03.012
ZHENG M X, SU H L, XIAO R F, et al. Pathogen of Bacterial Soft Rot on Paris polyphylla Smith var. chinensis (Franch.) Hara in Fujian [J]. Fujian Journal of Agricultural Sciences,2021,36(3):332−336 doi: 10.19303/j.issn.1008-0384.2021.03.012
Citation: ZHENG M X, SU H L, XIAO R F, et al. Pathogen of Bacterial Soft Rot on Paris polyphylla Smith var. chinensis (Franch.) Hara in Fujian [J]. Fujian Journal of Agricultural Sciences,2021,36(3):332−336 doi: 10.19303/j.issn.1008-0384.2021.03.012

福建省七叶一枝花细菌性软腐病病原鉴定

doi: 10.19303/j.issn.1008-0384.2021.03.012
基金项目: 福建省科技计划公益类专项(2019R1034-9);福建省农业科学院科技创新团队建设项目(STIT2017-2-8、kjfw20);福建省农业科学院科技服务项目(2021KF21-1);福建省科技厅对外合作项目 (2020I0034)
详细信息
    作者简介:

    郑梅霞(1986−),女,硕士,助理研究员,研究方向:农业生物资源保护与利用(E-mail:zhengmeixia2005@163.com

    通讯作者:

    朱育菁(1972−),女,博士,研究员,研究方向:农业生物资源保护与利用(E-mail:zyjingfz@163.com

  • 中图分类号: S 435

Pathogen of Bacterial Soft Rot on Paris polyphylla Smith var. chinensis (Franch.) Hara in Fujian

  • 摘要:   目的   调查七叶一枝花软腐病的发病特点及危害,分离和鉴定七叶一枝花软腐病的病原菌,以期明确七叶一枝花软腐病的病原菌,为该病的大田防治提供依据。   方法   通过对七叶一枝花软腐病进行田间病害调查和采样,以及对采集来的七叶一枝花样品进行病原菌分离纯化、形态特征鉴定、16S rDNA基因序列特征及致病性测定,综合分析鉴定七叶一枝花软腐病的病原菌。   结果   七叶一枝花软腐病的田间病害调查结果表明从每年2月植株出苗开始,七叶一枝花叶片、茎秆和块茎均有软腐病发生,常发于通气不良、田里积水环境。叶片发病由初期受害部位的水浸状的坏死有刺激性气味产生,而后感病部位内部组织被分解为液体状态,直至软腐。对发病植株上分离出的菌株进行形态学、分子生物学特征和致病性测定,将七叶一枝花软腐病病原菌鉴定为胡萝卜软腐果胶杆菌Pectobacterium carowvorum   结论   七叶一枝花在通气不良、排水不畅的环境中易感软腐病,胡萝卜软腐果胶杆菌P. carowvorum是七叶一枝花软腐病的病原菌。
  • 图  1  七叶一枝花植株软腐病发病症状

    注:a:田间发病症状;b:叶部发病症状;c:茎秆部发病症状;d:块茎部发病症状。

    Figure  1.  Symptoms of soft rot disease on P. polyphylla

    Note: a: Disease onset symptoms on plants in the field; b: Symptoms on leaves; c: Symptoms on stems; d: Symptoms on tubers.

    图  2  七叶一枝花植株人工接种致病症状.

    注:a、b.对照正常植株和回接发病植株;c.茎秆涂抹回接发病症状。

    Figure  2.  Symptoms of soft rot disease on artificially inoculated P. polyphylla plant

    Note: a and b: Reattached diseased and normal plants; c: Symptoms on reattached stalks smeared with disease.

    图  3  植株软腐病病原菌的菌落形态

    Figure  3.  Morphology of soft rot pathogen colonies

    图  4  植株软腐病病原细菌形态特征

    Figure  4.  Morphology of soft rot pathogen

    图  5  七叶一枝花植株软腐病病原细菌的发育树

    Figure  5.  Pathogenic bacteria development tree of soft rot disease on P. polyphylla

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出版历程
  • 收稿日期:  2020-11-07
  • 修回日期:  2020-12-19
  • 网络出版日期:  2021-03-27
  • 刊出日期:  2021-03-31

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