2011 Vol. 26, No. 1
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Display Method:
2011, 26(1): 1-5.
Abstract:
By using DNA recombination technology,nucleocapsid gene from swine transmissible gastroenteritis virus was lined with the gene fragment of the maximum local hydrophilic antigenic determinant of nucleocapsid gene from porcine epidemic diarrhea virus.The recombinant gene was cloned into the prokaryotic expression vector pET-32a(+).Then,the prokaryotic expression plasmid with the bivalent gene to prevent piglet’s coronaviral diarrhea was constructed.After transforming the plasmid into E.coil BL21(DE3),the bacteria were induced by IPTG with SDS-PAGE analysis showing the bivalent gene expressed efficiently,as well as,the western-blot test expressing the fusion protein.
By using DNA recombination technology,nucleocapsid gene from swine transmissible gastroenteritis virus was lined with the gene fragment of the maximum local hydrophilic antigenic determinant of nucleocapsid gene from porcine epidemic diarrhea virus.The recombinant gene was cloned into the prokaryotic expression vector pET-32a(+).Then,the prokaryotic expression plasmid with the bivalent gene to prevent piglet’s coronaviral diarrhea was constructed.After transforming the plasmid into E.coil BL21(DE3),the bacteria were induced by IPTG with SDS-PAGE analysis showing the bivalent gene expressed efficiently,as well as,the western-blot test expressing the fusion protein.
2011, 26(1): 6-9.
Abstract:
Layers from one of the chicken houses in Fujian showed egg laying reduction and some died.Hemangioma was observed on their claws.Using a real-time florescence quantitative polymerase chain reaction method,ALV-J was found in the tissue and blood samples of the infected layers.The sequence analysis further indicated that the viruses were highly homologous to those reported previously at home and abroad.This was the first time the existence of ALV-J in domestic layers in Fujian was confirmed by means of a pathogenic microbiological diagnostic method.
Layers from one of the chicken houses in Fujian showed egg laying reduction and some died.Hemangioma was observed on their claws.Using a real-time florescence quantitative polymerase chain reaction method,ALV-J was found in the tissue and blood samples of the infected layers.The sequence analysis further indicated that the viruses were highly homologous to those reported previously at home and abroad.This was the first time the existence of ALV-J in domestic layers in Fujian was confirmed by means of a pathogenic microbiological diagnostic method.
2011, 26(1): 10-12.
Abstract:
A pair of primers based on NS5 gene sequences of different strains of genus Flavivirus was designed for amplification of 470 bp fragments.No positive target fragment was available from H9 subtype influenza virus,Muscovy duck reovirus,duck plague,duck hepatitis virus(DHV-Ⅰ) or duck-origin avian paramyxovirus typeⅠ.The detection limit reached 20 pg under the optimized conditions.The results showed that the RT-PCR was sensitive,specific and applicable for rapid laboratory diagnosis and epidemiologic surveillance.
A pair of primers based on NS5 gene sequences of different strains of genus Flavivirus was designed for amplification of 470 bp fragments.No positive target fragment was available from H9 subtype influenza virus,Muscovy duck reovirus,duck plague,duck hepatitis virus(DHV-Ⅰ) or duck-origin avian paramyxovirus typeⅠ.The detection limit reached 20 pg under the optimized conditions.The results showed that the RT-PCR was sensitive,specific and applicable for rapid laboratory diagnosis and epidemiologic surveillance.
2011, 26(1): 24-28.
Abstract:
Rice codon optimized cry1Ca gene was cloned into expression vector pET-28b(+) by means of restricted enzymatic digestion.His-cry1Ca fusion protein was highly expressed by SDS-PAGE analysis accumulating more than 24% of the total bacterial proteins.The main expression production was deposited as inclusion body.His-cry1Ca was then purified with Ni-NTA after treatment of solvents.The target protein was used as the antigen to immunize rabbits.ELISA assay showed that the titer of the prepared polyclonal antibody was 1:50000,and had high specificities.
Rice codon optimized cry1Ca gene was cloned into expression vector pET-28b(+) by means of restricted enzymatic digestion.His-cry1Ca fusion protein was highly expressed by SDS-PAGE analysis accumulating more than 24% of the total bacterial proteins.The main expression production was deposited as inclusion body.His-cry1Ca was then purified with Ni-NTA after treatment of solvents.The target protein was used as the antigen to immunize rabbits.ELISA assay showed that the titer of the prepared polyclonal antibody was 1:50000,and had high specificities.
2011, 26(1): 29-32.
Abstract:
WRKY transcription factor family is one of its large kinds that involve in the biotic and abiotic responses.GmWRKY cDNA sequence,which had a 92% homology with Arabidopsis thaliana transcription factor WRKY14,was obtained by RT-PCR.Its expression pattern in different tissues and under abiotic stresses was analysed by semi-quantitative RT-PCR.The result showed that GmWRKY expressed in soybean roots,stems,as well as,leaves,and the expression was strongly induced by abiotic stresses,such as aluminum,P deficiency and salt.It suggested that GmWRKY could play an important role in stress resistance of soybean under abiotic stresses.
WRKY transcription factor family is one of its large kinds that involve in the biotic and abiotic responses.GmWRKY cDNA sequence,which had a 92% homology with Arabidopsis thaliana transcription factor WRKY14,was obtained by RT-PCR.Its expression pattern in different tissues and under abiotic stresses was analysed by semi-quantitative RT-PCR.The result showed that GmWRKY expressed in soybean roots,stems,as well as,leaves,and the expression was strongly induced by abiotic stresses,such as aluminum,P deficiency and salt.It suggested that GmWRKY could play an important role in stress resistance of soybean under abiotic stresses.
2011, 26(1): 33-44.
Abstract:
In order to improve the photosynthetic productivity of crops,an experimental site was set to observe solar radiation and photosynthetic production of crops in Longhai,Fujian from 2006 to 2009.The results showed that(1) based on solar radiation and sunshine rate,an empirical formula for calculating the monthly,seasonal radiation in Longhai was established;(2) a positive correlation between solar radiation and plant’s dry matter accumulation and economic yield was found-the solar radiation during the period between rice full heading and maturation had a significant and determining effect on the grain yield;(3) the average light energy utilization(Eu) rates were 2.10%,1.98% and 1.85%,respectively,in the entire growth period of Longhai early rice,the late rice and the potatoes,and in the short-term the highest Eu was 4.26%;and(4) estimated by the local annual solar radiation and the maximum Eu value,the greatest theoretical yields of the early rice,the late rice and the potatoes could be 1.6 to1.8 times higher than the current production of the three crops indicating a substantial production increase potential.By more efficient utilization of the solar energy,increase in crop yield could be realized.
In order to improve the photosynthetic productivity of crops,an experimental site was set to observe solar radiation and photosynthetic production of crops in Longhai,Fujian from 2006 to 2009.The results showed that(1) based on solar radiation and sunshine rate,an empirical formula for calculating the monthly,seasonal radiation in Longhai was established;(2) a positive correlation between solar radiation and plant’s dry matter accumulation and economic yield was found-the solar radiation during the period between rice full heading and maturation had a significant and determining effect on the grain yield;(3) the average light energy utilization(Eu) rates were 2.10%,1.98% and 1.85%,respectively,in the entire growth period of Longhai early rice,the late rice and the potatoes,and in the short-term the highest Eu was 4.26%;and(4) estimated by the local annual solar radiation and the maximum Eu value,the greatest theoretical yields of the early rice,the late rice and the potatoes could be 1.6 to1.8 times higher than the current production of the three crops indicating a substantial production increase potential.By more efficient utilization of the solar energy,increase in crop yield could be realized.
2011, 26(1): 45-49.
Abstract:
This study analyzed 15 agronomic trait indicators by hierarchical clustering method,and built inherit distance tree for Dioscorea polystachya Turcz.When the absolute value was between 13 and 26,34 D.polystachya Turcz.were divided into two categories according to their state of above ground growth.When the absolute value was between 9 and 13,the category of the poorer growth was further divided into two sub-categories:(a) the long D.polystachya Turcz.with branches and russet brown bark,and(b) rod-like D.polystachya Turcz.and lisbon yam.When the absolute value was around 8,the rod-like D.polystachya Turcz.and D.alata L.became distinguishable.The two methods could satisfactorily separate D.alata L.from the general D.polystachya Turcz.,according to the analytical results of comprehensive morphological and molecular markers.It also showed that the tuber type could be an important criterion for distinguishing different varieties of D.polystachya Turcz.
This study analyzed 15 agronomic trait indicators by hierarchical clustering method,and built inherit distance tree for Dioscorea polystachya Turcz.When the absolute value was between 13 and 26,34 D.polystachya Turcz.were divided into two categories according to their state of above ground growth.When the absolute value was between 9 and 13,the category of the poorer growth was further divided into two sub-categories:(a) the long D.polystachya Turcz.with branches and russet brown bark,and(b) rod-like D.polystachya Turcz.and lisbon yam.When the absolute value was around 8,the rod-like D.polystachya Turcz.and D.alata L.became distinguishable.The two methods could satisfactorily separate D.alata L.from the general D.polystachya Turcz.,according to the analytical results of comprehensive morphological and molecular markers.It also showed that the tuber type could be an important criterion for distinguishing different varieties of D.polystachya Turcz.
2011, 26(1): 55-59.
Abstract:
Breeding and main characteristics of Quanshu No.9,a new sweetpotato variety,are preliminarily presented in this report in order to provide references for its potential production and breeding applications.Quanshu No.9 was bred from the female parent,Quanshu 268,by open-pollination by Quanzhou Institute of Agricultural Science in 2003.Its innovative and successful breeding method of using middle-materials as parents of composite hybridization through single cross,group cross or open-pollination was effective for sweetpotato breeding.Trials at multiple-spots in different years showed that Quanshu No.9 had a high yield,and its dry root and starch were especially high and stable.The annual yield of the dry root was between 8.50 and 14.93 t·hm-2,being 104.20% to 152.30% of those of the reference varieties.The annual yield of starch was between 5.62 and 9.85 t.hm-2,being 107.91 to 155.36% of those of the reference varieties.The annual yield of starch was between 6.43 and 9.85 t·hm-2,being 109.75% to 155.36% of those of the reference varieties.The dry matter and starch contents of the fresh root was about 30.05% and 20.5%,respectively,i.e.,3.5%-4.0% higher than those of the reference varieties.The edible and sensory quality of the sweetpotatoes was better than that of Jinshang 57,and equivalent to that of Nanshu 88.Its external appearance was better than the reference varieties’.This variety was resistant to fusarium wilt,bacterial wilt,black spot,and stem nematode.And,among other superior characteristics,it had desirable storage performance and germinating ability.Quanshu No.9 was suitable for either fresh-eating or starch-processing.It was examined and approved by the Crop Variety Approval Committee of Fujian Province.Its acreage in Fujian was about 3000 hm2 in 2009,and the prospect for large production appeared to be bright in Southern and Middle China,as well as,the Lower Reaches of Yangtze River.
Breeding and main characteristics of Quanshu No.9,a new sweetpotato variety,are preliminarily presented in this report in order to provide references for its potential production and breeding applications.Quanshu No.9 was bred from the female parent,Quanshu 268,by open-pollination by Quanzhou Institute of Agricultural Science in 2003.Its innovative and successful breeding method of using middle-materials as parents of composite hybridization through single cross,group cross or open-pollination was effective for sweetpotato breeding.Trials at multiple-spots in different years showed that Quanshu No.9 had a high yield,and its dry root and starch were especially high and stable.The annual yield of the dry root was between 8.50 and 14.93 t·hm-2,being 104.20% to 152.30% of those of the reference varieties.The annual yield of starch was between 5.62 and 9.85 t.hm-2,being 107.91 to 155.36% of those of the reference varieties.The annual yield of starch was between 6.43 and 9.85 t·hm-2,being 109.75% to 155.36% of those of the reference varieties.The dry matter and starch contents of the fresh root was about 30.05% and 20.5%,respectively,i.e.,3.5%-4.0% higher than those of the reference varieties.The edible and sensory quality of the sweetpotatoes was better than that of Jinshang 57,and equivalent to that of Nanshu 88.Its external appearance was better than the reference varieties’.This variety was resistant to fusarium wilt,bacterial wilt,black spot,and stem nematode.And,among other superior characteristics,it had desirable storage performance and germinating ability.Quanshu No.9 was suitable for either fresh-eating or starch-processing.It was examined and approved by the Crop Variety Approval Committee of Fujian Province.Its acreage in Fujian was about 3000 hm2 in 2009,and the prospect for large production appeared to be bright in Southern and Middle China,as well as,the Lower Reaches of Yangtze River.
2011, 26(1): 13-18.
Abstract:
A 28 day-feeding experiment was conducted to determine the effects of the functional egg yolk powder as a feed ingredient on the growth performance,diarrhea occurrence,fecal microbe and the morphology of intestinal villi of the piglets.The results showed that(a) the diarrhea occurrence rates among the piglets in GroupⅠand Ⅱdecreased by 54.8%(P0.01) and 84.3%(P0.01),respectively,as compared to the control;and,(b) the fecal Escherichia coli at the 28th days decreased in GroupⅠ(P0.01),while decreased during the entire testing period in GroupⅡ(P0.01).The heights of the duodenum villi of piglets in GroupⅠand Ⅱwere greater than those in the control group(P0.01).The average daily piglet weight gain was significantly higher in both Group I and II than that of Control Group(P0.05),increased by 14.4% and 15.3%,respectively.The average daily feed intakes of the piglets in GroupⅠand Ⅱwere higher than that of the control(P0.05) by 11.70% and 6.86%,respectively.The feed conversion efficiencies for GroupⅠand Ⅱwere lower than that of the control(P0.05) by 5.55% and 6.50%,respectively.The results indicated that addition of the functional egg yolk powder in the feed could effectively inhibit the growth of the enterotoxigenic E.coli preventing piglets from diarrhea and improve their growth performances.
A 28 day-feeding experiment was conducted to determine the effects of the functional egg yolk powder as a feed ingredient on the growth performance,diarrhea occurrence,fecal microbe and the morphology of intestinal villi of the piglets.The results showed that(a) the diarrhea occurrence rates among the piglets in GroupⅠand Ⅱdecreased by 54.8%(P0.01) and 84.3%(P0.01),respectively,as compared to the control;and,(b) the fecal Escherichia coli at the 28th days decreased in GroupⅠ(P0.01),while decreased during the entire testing period in GroupⅡ(P0.01).The heights of the duodenum villi of piglets in GroupⅠand Ⅱwere greater than those in the control group(P0.01).The average daily piglet weight gain was significantly higher in both Group I and II than that of Control Group(P0.05),increased by 14.4% and 15.3%,respectively.The average daily feed intakes of the piglets in GroupⅠand Ⅱwere higher than that of the control(P0.05) by 11.70% and 6.86%,respectively.The feed conversion efficiencies for GroupⅠand Ⅱwere lower than that of the control(P0.05) by 5.55% and 6.50%,respectively.The results indicated that addition of the functional egg yolk powder in the feed could effectively inhibit the growth of the enterotoxigenic E.coli preventing piglets from diarrhea and improve their growth performances.
2011, 26(1): 19-23.
Abstract:
Eels(Anguilla japonica) were randomly divided into the infected and control groups.Fish in the infected group were intraperitoneally injected with Aeromonas hydrophila suspension in the concentration of 3106 CFUmL-1,while those in the control group the same amount of sterile saline solution(0.85% NaCl).The eels were sacrificed to determine their nonspecific immunity on parameters including haematocyte count(i.e.,erythrocyte,leucocyte,lymphocyte and granulocyte),leucocyte phagocytic activity and serum antibacterial activity.The results showed that(a) the A.hydrophila infection increased the numbers of leucocytes and lymphocytes in the peripheral blood of A.japonica,and significantly reduced the number of granulocytes;(b) the phagocytic index and phagocytic rate increased on the 1st day after injection,and were very significantly different(P0.01) or significantly different(P0.05) on the 5th day and 9th day,as compared with the control;(c) on the 1st day after injection,the antibacterial activity of the infected fish also increased,and on the 9th day and 13th day,it was very significantly different(P0.01) from the control.The results suggested that the intraperitoneal injection enhanced A.japonicas resistance to the invading A.hydrophila by means of lymphocyte,especially,the proliferation of leucocytes,the increase in leucocyte phagocytic activity and the excretion of antibacterial substances.
Eels(Anguilla japonica) were randomly divided into the infected and control groups.Fish in the infected group were intraperitoneally injected with Aeromonas hydrophila suspension in the concentration of 3106 CFUmL-1,while those in the control group the same amount of sterile saline solution(0.85% NaCl).The eels were sacrificed to determine their nonspecific immunity on parameters including haematocyte count(i.e.,erythrocyte,leucocyte,lymphocyte and granulocyte),leucocyte phagocytic activity and serum antibacterial activity.The results showed that(a) the A.hydrophila infection increased the numbers of leucocytes and lymphocytes in the peripheral blood of A.japonica,and significantly reduced the number of granulocytes;(b) the phagocytic index and phagocytic rate increased on the 1st day after injection,and were very significantly different(P0.01) or significantly different(P0.05) on the 5th day and 9th day,as compared with the control;(c) on the 1st day after injection,the antibacterial activity of the infected fish also increased,and on the 9th day and 13th day,it was very significantly different(P0.01) from the control.The results suggested that the intraperitoneal injection enhanced A.japonicas resistance to the invading A.hydrophila by means of lymphocyte,especially,the proliferation of leucocytes,the increase in leucocyte phagocytic activity and the excretion of antibacterial substances.
2011, 26(1): 50-54.
Abstract:
It used the orthogonal regression rotary combinative design of 4 factors and five levels for the experiment in Longhai of Fujian.The high quality potato,Zhoushu 3,was employed to evaluate the production effect by varying planting density,as well as nitrogen,phosphate and potassium fertilizations.The result showed that the effect of the four factors decreased in that order.The mathematical models allowed the selection of a potato with a yield greater than 35832.75 kgm-2 at 95% confidence level.In addition,the range for the planting density was 70245 to 72435 strainm-2,the urea application,349.79 to 384.89 kgm-2,the calcium superphosphate application,461.81 to 558.20 kgm-2,and the potassium sulfate application,550.56 to 621.60 kgm-2.For agronomic practices,the medium values for the planting density was 71 340 strainm-2,and for the urea,calcium superphosphate and potassium sulfate application rates were 367.34 kgm-2,510.00 kgm-2 and 586.08 kgm-2,respectively.
It used the orthogonal regression rotary combinative design of 4 factors and five levels for the experiment in Longhai of Fujian.The high quality potato,Zhoushu 3,was employed to evaluate the production effect by varying planting density,as well as nitrogen,phosphate and potassium fertilizations.The result showed that the effect of the four factors decreased in that order.The mathematical models allowed the selection of a potato with a yield greater than 35832.75 kgm-2 at 95% confidence level.In addition,the range for the planting density was 70245 to 72435 strainm-2,the urea application,349.79 to 384.89 kgm-2,the calcium superphosphate application,461.81 to 558.20 kgm-2,and the potassium sulfate application,550.56 to 621.60 kgm-2.For agronomic practices,the medium values for the planting density was 71 340 strainm-2,and for the urea,calcium superphosphate and potassium sulfate application rates were 367.34 kgm-2,510.00 kgm-2 and 586.08 kgm-2,respectively.
