2013 Vol. 28, No. 12
Display Method:
2013, 28(12): 1195-1199.
doi: 10.19303/j.issn.1008-0384.2013.12.001
Abstract:
Effects of expression strains, plasmid stability and inducing conditions were studied on improving the expression efficiency of Cecropin AD and Buforin Ⅱ fusion protein in recombinant Escherichia coli.According to the result of SDS-PAGE, E.coli BER2566was accepted as expression strain.Plasmid pET (K) -Trx-CAD-Buforin Ⅱ with kanamycin resistant gene was constructed to improve the plasmid stability and protein expression.Inducing conditions including IPTG concentration, induction time and inducing time length were optimized.With the optimal conditions, the highest expression level of fusion protein was obtained in recombinant E.coli at the level of OD600 0.8and final IPTG concentration of 0.8mmol·L-1 for 5hinducing, which was more than 50%of the total protein in E.coli.
Effects of expression strains, plasmid stability and inducing conditions were studied on improving the expression efficiency of Cecropin AD and Buforin Ⅱ fusion protein in recombinant Escherichia coli.According to the result of SDS-PAGE, E.coli BER2566was accepted as expression strain.Plasmid pET (K) -Trx-CAD-Buforin Ⅱ with kanamycin resistant gene was constructed to improve the plasmid stability and protein expression.Inducing conditions including IPTG concentration, induction time and inducing time length were optimized.With the optimal conditions, the highest expression level of fusion protein was obtained in recombinant E.coli at the level of OD600 0.8and final IPTG concentration of 0.8mmol·L-1 for 5hinducing, which was more than 50%of the total protein in E.coli.
2013, 28(12): 1200-1203.
doi: 10.19303/j.issn.1008-0384.2013.12.002
Abstract:
Duck tembusu viruses were cultivated and concentrated by ultracentrifugation and density-gradient centrifugation to determine the best suitable conditions for sensitized latex and prepare antigen for the latex agglutination test (LAT) by titration.The agglutination particles could be observed clearly by naked eyes after antigen reaction with positive duck tembusu virus serum.The sensitized antigen had no cross-reaction with avian influenza virus, avian paramyxovirus type 1, duck hepatitis virus type 1, egg drop syndrome virus, and duck reovirus.Of 80duck serums, 58and 68 were detected to be positive by the established LAT and ELISA, respectively;the positive rates for both were above 85.3% with no statistical difference.The results revealed that the established LAT was simple, quick and specific for the rapid diagnose and survey of duck tembusu virus antibody.
Duck tembusu viruses were cultivated and concentrated by ultracentrifugation and density-gradient centrifugation to determine the best suitable conditions for sensitized latex and prepare antigen for the latex agglutination test (LAT) by titration.The agglutination particles could be observed clearly by naked eyes after antigen reaction with positive duck tembusu virus serum.The sensitized antigen had no cross-reaction with avian influenza virus, avian paramyxovirus type 1, duck hepatitis virus type 1, egg drop syndrome virus, and duck reovirus.Of 80duck serums, 58and 68 were detected to be positive by the established LAT and ELISA, respectively;the positive rates for both were above 85.3% with no statistical difference.The results revealed that the established LAT was simple, quick and specific for the rapid diagnose and survey of duck tembusu virus antibody.
2013, 28(12): 1204-1207.
doi: 10.19303/j.issn.1008-0384.2013.12.003
Abstract:
In order to understand the egg production rule of Huainan partridge chicken, the egg production curve of Huainan partridge chicken from 22to 60weeks old was analyzed and fitted with four mathematical models of Wood, McMillan, Yang and Ali-Schaeffer.The results showed that, the goodness of fit (R2) of four models were different and ranged from 0.391to 0.975.Wood model was not appropriate to predict the variation for egg production curve of caged Huainan partridge chicken (R2=0.391) .The egg production curves could be fitted well with three models of McMillan, Yang and Ali-Schaeffer, the fitness of Yang model was the highest of all (R2=0.975) .The result indicated that Yang model was the optimum model of egg production for caged Huainan partridge chicken.
In order to understand the egg production rule of Huainan partridge chicken, the egg production curve of Huainan partridge chicken from 22to 60weeks old was analyzed and fitted with four mathematical models of Wood, McMillan, Yang and Ali-Schaeffer.The results showed that, the goodness of fit (R2) of four models were different and ranged from 0.391to 0.975.Wood model was not appropriate to predict the variation for egg production curve of caged Huainan partridge chicken (R2=0.391) .The egg production curves could be fitted well with three models of McMillan, Yang and Ali-Schaeffer, the fitness of Yang model was the highest of all (R2=0.975) .The result indicated that Yang model was the optimum model of egg production for caged Huainan partridge chicken.
2013, 28(12): 1208-1212.
doi: 10.19303/j.issn.1008-0384.2013.12.004
Abstract:
To investigate the infection of red sea bream iridoviral disease (RSIVD) , 25batches of fish collected from coasta1areas of Fujian Province were examined by PCR method, the positive amplified fragments and MCP gene of positive virus strains were sequenced and matched with the reported Iridoviridae MCP gene in Genbank.The result showed that two RSIVD strains was detected in samples from large yellow croaker and hapalogenys nitens respectively, which were named RISV-FJ-DHY and RISV-FJ-XDZD and found belonging to Megalocytivirus genus of Iridoviridae.Mutations were found in RISV-FJ-DHY and the strains isolated from large yellow croaker in 2002, while RISV-FJ-XDZD was genetically stable.
To investigate the infection of red sea bream iridoviral disease (RSIVD) , 25batches of fish collected from coasta1areas of Fujian Province were examined by PCR method, the positive amplified fragments and MCP gene of positive virus strains were sequenced and matched with the reported Iridoviridae MCP gene in Genbank.The result showed that two RSIVD strains was detected in samples from large yellow croaker and hapalogenys nitens respectively, which were named RISV-FJ-DHY and RISV-FJ-XDZD and found belonging to Megalocytivirus genus of Iridoviridae.Mutations were found in RISV-FJ-DHY and the strains isolated from large yellow croaker in 2002, while RISV-FJ-XDZD was genetically stable.
2013, 28(12): 1213-1217.
doi: 10.19303/j.issn.1008-0384.2013.12.005
Abstract:
Productivity of a new Daiyun Goat Strain which consecutively breeded by four generations were researched.Comparing with the generation Zero, the new Daiyun Goat Strain weight has increased from 2.26%to 14.06%, and the chest girth incretion was 1.39%-4.20%, while height at withers, body length, chest width and chest depth have not increased significantly.The breeding rate and conception rate of each generation were all above 90%;and the lambing number of the first-born in each generation were between 1.32-1.48, which of the laterborn in each generations were between 1.89-2.12.
Productivity of a new Daiyun Goat Strain which consecutively breeded by four generations were researched.Comparing with the generation Zero, the new Daiyun Goat Strain weight has increased from 2.26%to 14.06%, and the chest girth incretion was 1.39%-4.20%, while height at withers, body length, chest width and chest depth have not increased significantly.The breeding rate and conception rate of each generation were all above 90%;and the lambing number of the first-born in each generation were between 1.32-1.48, which of the laterborn in each generations were between 1.89-2.12.
2013, 28(12): 1218-1223.
doi: 10.19303/j.issn.1008-0384.2013.12.006
Abstract:
Tianyou 3301was developed by crossing the good quality three-line male sterile“Tianfeng A”with the restorer line“Minhui 3301”.The cultivated area of Tianyou 3301is 300 000hm2from 2010to 2012, the total yields were about 150million kg in three years.It is characteristic of good plant types, super-high yield, good quality, blast resistance, wide adaptability, large panicles and more spikelets, high setting rate, strong ratoon ability and high temperature tolerance at heading stage.It was registered in Fujian province and the National Crops Variety Approval Committee in 2008and 2010, respectively, and was approved in Hainan province in 2011.It was one of those super-hybrid varieties to be demonstrated and extended in national wide.The extension and application, botanical traits and yield components of Tianyou 3301were summarized in this paper.And its yield, resistance and quality in regional trials, producing trials and high yield demonstration were introduced in the paper.
Tianyou 3301was developed by crossing the good quality three-line male sterile“Tianfeng A”with the restorer line“Minhui 3301”.The cultivated area of Tianyou 3301is 300 000hm2from 2010to 2012, the total yields were about 150million kg in three years.It is characteristic of good plant types, super-high yield, good quality, blast resistance, wide adaptability, large panicles and more spikelets, high setting rate, strong ratoon ability and high temperature tolerance at heading stage.It was registered in Fujian province and the National Crops Variety Approval Committee in 2008and 2010, respectively, and was approved in Hainan province in 2011.It was one of those super-hybrid varieties to be demonstrated and extended in national wide.The extension and application, botanical traits and yield components of Tianyou 3301were summarized in this paper.And its yield, resistance and quality in regional trials, producing trials and high yield demonstration were introduced in the paper.
2013, 28(12): 1224-1228.
doi: 10.19303/j.issn.1008-0384.2013.12.007
Abstract:
Uniform design was used to analyze the cultivation of the high yield, hybrid rice, Guyoumingzhan, based on three factors:seedling age (X1) , planting density (X2) and quantity of nitrogen (X3) .The regression models for the three factors affecting the rice yield were established.The experimental results revealed that the three factors exerted significant influences on the yield, After a simulated screening and optimization by computer, seedling age in the range of 25.4-29.7dfor transplanting at the density of 301 000-332 000hills·hm-2 with a nitrogen application of 183.8-210.0kg·hm-2 was recommended for the cultivation on yield≥8 250kg·hm-2.
Uniform design was used to analyze the cultivation of the high yield, hybrid rice, Guyoumingzhan, based on three factors:seedling age (X1) , planting density (X2) and quantity of nitrogen (X3) .The regression models for the three factors affecting the rice yield were established.The experimental results revealed that the three factors exerted significant influences on the yield, After a simulated screening and optimization by computer, seedling age in the range of 25.4-29.7dfor transplanting at the density of 301 000-332 000hills·hm-2 with a nitrogen application of 183.8-210.0kg·hm-2 was recommended for the cultivation on yield≥8 250kg·hm-2.
Autumn Maize Varieties Screening Test and Correlation Analysis of Main Quantitative Traits in Fujian
2013, 28(12): 1229-1233.
doi: 10.19303/j.issn.1008-0384.2013.12.008
Abstract:
In this study, in order to select the suitable autumn planting maize varieties in Fujian, we introduced a lots varieties of maize from different ecological types, and tested them through years and multi-point testing in the autumn.Moreover, we carried out the correlation and path analysis of the main quantitative characteristics and yield on maize, in order to study the method of breeding high yield of autumn planting maize.The results showed that denghai3622, derui29and sushi50103had high and stable yield and good resistance adapted to autumn planted in Fujian.In the strategy of breeding high yield autumn planting maize, it′s important to select the varieties of resistant to south-rust disease, and take into account of ear length and hundred-grain weight increase. Furthoremore, the growth season for maize should moderate, but the long growth season is not inadvisable.
In this study, in order to select the suitable autumn planting maize varieties in Fujian, we introduced a lots varieties of maize from different ecological types, and tested them through years and multi-point testing in the autumn.Moreover, we carried out the correlation and path analysis of the main quantitative characteristics and yield on maize, in order to study the method of breeding high yield of autumn planting maize.The results showed that denghai3622, derui29and sushi50103had high and stable yield and good resistance adapted to autumn planted in Fujian.In the strategy of breeding high yield autumn planting maize, it′s important to select the varieties of resistant to south-rust disease, and take into account of ear length and hundred-grain weight increase. Furthoremore, the growth season for maize should moderate, but the long growth season is not inadvisable.
2013, 28(12): 1234-1239.
doi: 10.19303/j.issn.1008-0384.2013.12.009
Abstract:
To test the effects of different doses and fertilizer regimes of molybdenum on leaf size, economic characters and conventional chemical composition of flue-cured tobacco, field experiments were conducted in Changshun area.The results showed that the molybdenum could increase the the blade size, yield and production of tobacco, and reduce the proportion of variegated tobacco significantly;and molybdenum fertilization could increase the content of the potassium and reduce the content of the nicotine within tobacco leaves.The effect of foliar spraying on the leaves or combination of watering the root and spraying were much better than that of watering root once when transplanting.
To test the effects of different doses and fertilizer regimes of molybdenum on leaf size, economic characters and conventional chemical composition of flue-cured tobacco, field experiments were conducted in Changshun area.The results showed that the molybdenum could increase the the blade size, yield and production of tobacco, and reduce the proportion of variegated tobacco significantly;and molybdenum fertilization could increase the content of the potassium and reduce the content of the nicotine within tobacco leaves.The effect of foliar spraying on the leaves or combination of watering the root and spraying were much better than that of watering root once when transplanting.
2013, 28(12): 1240-1243.
doi: 10.19303/j.issn.1008-0384.2013.12.010
Abstract:
Pot experiments with hydroponics were carried out to study the effect of adventitious root on the chlorophyll content, nitrate reductase activity and the content of nitrogen, potassium, nicotine in K326flue-cured tobacco after multi-topping.The results showed that the chlorophyll content, nitrate reductase activity and the content of nitrogen, potassium, nicotine were greatly enhanced after adventitious root induced by flower mud.It indicated that adventitious root could absorb more nutrients and promote growth of tobacco.Comparing with other treatments, the effect of adding nutrient solution directly to the flower mud was the best, especially in the chlorophyll content, nitrate reductase activity and the content of nitrogen, potassium from cultured plants, and the content of nicotine was appropriate.
Pot experiments with hydroponics were carried out to study the effect of adventitious root on the chlorophyll content, nitrate reductase activity and the content of nitrogen, potassium, nicotine in K326flue-cured tobacco after multi-topping.The results showed that the chlorophyll content, nitrate reductase activity and the content of nitrogen, potassium, nicotine were greatly enhanced after adventitious root induced by flower mud.It indicated that adventitious root could absorb more nutrients and promote growth of tobacco.Comparing with other treatments, the effect of adding nutrient solution directly to the flower mud was the best, especially in the chlorophyll content, nitrate reductase activity and the content of nitrogen, potassium from cultured plants, and the content of nicotine was appropriate.
2013, 28(12): 1244-1247.
doi: 10.19303/j.issn.1008-0384.2013.12.011
Abstract:
Mintang 01-77was a new sugarcane variety, a cross between ROC20and YC84-153.It was high on yield and sucrose content, and widely adaptable.The results from various farming sites for several years showed that it had the characteristics of good germination, strong tillering, good ratooning, and a high mean cane yield of 122 257 kg·hm-2 (i.e., 5.24% higher than the control) .Its sucrose content was 14.88%in December, and sugar yield was 18.197t·hm-2 (i.e., 4.43% higher than the standard control for Fujian) .
Mintang 01-77was a new sugarcane variety, a cross between ROC20and YC84-153.It was high on yield and sucrose content, and widely adaptable.The results from various farming sites for several years showed that it had the characteristics of good germination, strong tillering, good ratooning, and a high mean cane yield of 122 257 kg·hm-2 (i.e., 5.24% higher than the control) .Its sucrose content was 14.88%in December, and sugar yield was 18.197t·hm-2 (i.e., 4.43% higher than the standard control for Fujian) .
2013, 28(12): 1248-1251.
doi: 10.19303/j.issn.1008-0384.2013.12.012
Abstract:
'Minyin huangpei sugarcane'is a new sugarcane variety introduced from Guangdong.This variety characterizes excellently in plant height and stem diameter with high yield, good in quality and textures.By repeating annual multipoint test for the variety demonstration in production, its average yield was 142 653.3 kg·hm-2 and was 20.37% higher than the yield of Heipi sugarcane′in 118 508.7kg·m-2 (P<0.01) .Some agronomic properties and quality traits of the variety were similar or better than the control variety, which indicated apotential of it in extending for cultivation.
'Minyin huangpei sugarcane'is a new sugarcane variety introduced from Guangdong.This variety characterizes excellently in plant height and stem diameter with high yield, good in quality and textures.By repeating annual multipoint test for the variety demonstration in production, its average yield was 142 653.3 kg·hm-2 and was 20.37% higher than the yield of Heipi sugarcane′in 118 508.7kg·m-2 (P<0.01) .Some agronomic properties and quality traits of the variety were similar or better than the control variety, which indicated apotential of it in extending for cultivation.
2013, 28(12): 1252-1256.
doi: 10.19303/j.issn.1008-0384.2013.12.013
Abstract:
Three-year-old Xiahei were used in studying for the effects of three different calcium fertilizers on the quality and storage property of the grapes.The results showed that applying calcium fertilizers increased the content of soluble solids, total sugar and vitamin C in the fruits significantly while decreased the fruit acidity;the duration of the fruit storage was prolonged.Comparing three fertilizers, spraying fluid fertilizer showed the best effects.By the end of the experiment date (August 13th) , the texture of fruit in hardness, the content of soluble solids, total sugar and vitamin C of the fruits from liquid calcium treatment were increased comparatively to those in the contrast for 9.25%, 19.14%, 20.70%and 16.49, respectively, with 20.99%reduction in acidity and 12.02%increase in solid acid ratio of treated fruits.The results showed that the treatment improved the fruit quality obviously.
Three-year-old Xiahei were used in studying for the effects of three different calcium fertilizers on the quality and storage property of the grapes.The results showed that applying calcium fertilizers increased the content of soluble solids, total sugar and vitamin C in the fruits significantly while decreased the fruit acidity;the duration of the fruit storage was prolonged.Comparing three fertilizers, spraying fluid fertilizer showed the best effects.By the end of the experiment date (August 13th) , the texture of fruit in hardness, the content of soluble solids, total sugar and vitamin C of the fruits from liquid calcium treatment were increased comparatively to those in the contrast for 9.25%, 19.14%, 20.70%and 16.49, respectively, with 20.99%reduction in acidity and 12.02%increase in solid acid ratio of treated fruits.The results showed that the treatment improved the fruit quality obviously.
2013, 28(12): 1257-1262.
doi: 10.19303/j.issn.1008-0384.2013.12.014
Abstract:
Fresh leaves of Mingke 1were processed with traditional processing technology for green tea, black tea, white tea and oolong tea, respectively, and stream tea sample used for control.The aromatic components of various tea samples were detected with GC-MS.The results showed that the effect of processing techniques on the aromatic components and quality of tea samples were great.The green tea of Mingke 1had flavor like chestnut mixed with flower, and the major aromatic compounds wereβ-linalool, cis-linalool oxide, trans-geranilo and hotrienol.Its black tea had honey peach-like smell, and the major aromatic compounds were D-merolidol, trans-geranilo, cis-hexanic acid 3-hexenyl ester, cis-jasmone and cis-linalool oxide.The Mingke 1white tea had orchid-like aroma with major aromatic compounds in trans-geranilo, cislinalool oxide, salicylic acid methyl ester andβ-linalool.The oolong tea gave the aromatic smell of orchid-like and gardenialike, and the major aromatic compounds were D-merolidol, indloe, cis-β-ocimene and phenylethyl alcohol.The quality of green tea, black tea, white tea and oolong tea processed from Mingke 1 was excellent although their flavors and the aromatic components were different.The results indicated that Mingke 1was suited in processing for various types of tea differently with distinctive and rich in flavors.
Fresh leaves of Mingke 1were processed with traditional processing technology for green tea, black tea, white tea and oolong tea, respectively, and stream tea sample used for control.The aromatic components of various tea samples were detected with GC-MS.The results showed that the effect of processing techniques on the aromatic components and quality of tea samples were great.The green tea of Mingke 1had flavor like chestnut mixed with flower, and the major aromatic compounds wereβ-linalool, cis-linalool oxide, trans-geranilo and hotrienol.Its black tea had honey peach-like smell, and the major aromatic compounds were D-merolidol, trans-geranilo, cis-hexanic acid 3-hexenyl ester, cis-jasmone and cis-linalool oxide.The Mingke 1white tea had orchid-like aroma with major aromatic compounds in trans-geranilo, cislinalool oxide, salicylic acid methyl ester andβ-linalool.The oolong tea gave the aromatic smell of orchid-like and gardenialike, and the major aromatic compounds were D-merolidol, indloe, cis-β-ocimene and phenylethyl alcohol.The quality of green tea, black tea, white tea and oolong tea processed from Mingke 1 was excellent although their flavors and the aromatic components were different.The results indicated that Mingke 1was suited in processing for various types of tea differently with distinctive and rich in flavors.
2013, 28(12): 1263-1266.
doi: 10.19303/j.issn.1008-0384.2013.12.015
Abstract:
Using fruit fly (Drosophila melanogaster) survival experiment, we investigated the antioxidant and antiaging functions of grapefruit juice in vitro.Low dose (5%) 、middle dose (10%) and high dose (20%) of grapefruit juice were added into the basic medium for fruit fly respectively, the average lifespan and the highest lifespan of the fruit flies fed in three groups were calculated.Moreover, SOD activity and MDA content in the homogenate of fruit fly body were determined.The results showed that, with low, middle and high dosages, the grapefruit juice significantly prolonged the average lifespan of male fruit flies by 8.24%, 20.06%and 13.50%, respectively, while 12.04%, 24.93% and 19.33% for it of the female fruit flies, respectively.Feeding grapefruit juice in certain concentrate could improve SOD activity and reduce MDA content for both male and female fruit flies.The data from middle dose group showed the best effects which coincide with the results of fruit fly survival experiment.
Using fruit fly (Drosophila melanogaster) survival experiment, we investigated the antioxidant and antiaging functions of grapefruit juice in vitro.Low dose (5%) 、middle dose (10%) and high dose (20%) of grapefruit juice were added into the basic medium for fruit fly respectively, the average lifespan and the highest lifespan of the fruit flies fed in three groups were calculated.Moreover, SOD activity and MDA content in the homogenate of fruit fly body were determined.The results showed that, with low, middle and high dosages, the grapefruit juice significantly prolonged the average lifespan of male fruit flies by 8.24%, 20.06%and 13.50%, respectively, while 12.04%, 24.93% and 19.33% for it of the female fruit flies, respectively.Feeding grapefruit juice in certain concentrate could improve SOD activity and reduce MDA content for both male and female fruit flies.The data from middle dose group showed the best effects which coincide with the results of fruit fly survival experiment.
2013, 28(12): 1267-1272.
doi: 10.19303/j.issn.1008-0384.2013.12.016
Abstract:
51samples of sweet potato from Fujian province were detected by the double antibody enzyme-linked immunosorbent method (DAS-ELISA) .The results showed that 24samples were SPFMV-positive, the positive rate were 47.06%;the highest positive rate was 71.40%, detected in the samples from Quanzhou, and the next were 50.00%, detected in samples from Nanan and Longyan.A pair of primer was designed and synthesized according to the published nucleotide sequence of coat protein gene of SPFMV, and a specific RT-PCR detection procedure was established.The amplified SPFMV-specific fragment in PCR contained 441bps, and showed 92%-97%, homology with the SPFMV reference sequence.
51samples of sweet potato from Fujian province were detected by the double antibody enzyme-linked immunosorbent method (DAS-ELISA) .The results showed that 24samples were SPFMV-positive, the positive rate were 47.06%;the highest positive rate was 71.40%, detected in the samples from Quanzhou, and the next were 50.00%, detected in samples from Nanan and Longyan.A pair of primer was designed and synthesized according to the published nucleotide sequence of coat protein gene of SPFMV, and a specific RT-PCR detection procedure was established.The amplified SPFMV-specific fragment in PCR contained 441bps, and showed 92%-97%, homology with the SPFMV reference sequence.
2013, 28(12): 1273-1276.
doi: 10.19303/j.issn.1008-0384.2013.12.017
Abstract:
The comparison in field trapping efficacy on B.dorsalis was carried out for zhenfengkexing sticky-trap and 3attractants such as Yi′brand Herbal aromatic attractant (Yi′brand) , Haotianyuan′physical induced adhesive (Haotianyuan′) and Methyl eugenol, in red meat honey pomelo orchard.The results showed that the numbers of B. dorsalis attracted by Haotianyuan′was maximal in overall trial (30d) and single-day for (114.8±27.7) and (15.0 ±3.9) head·bottler-1, respectively, which were significantly higher than those of‘Yi'brand, Methyl eugenol and Zhenfengkexing sticky-trap.The differences in field trapping efficacy of 4attracts were remarkable for various trapping time schedule as showed in following: (1) the highest trapping number of B.dorsalis was obtained by Haotianyuan′in two time phases of the 1-5dand 6-10dafter treatment; (2) during the 20-25dafter treatment, the trapping number was significantly lower in Haotianyuan′than in Yi′brand; (3) there was no significant difference in attracting quantity between Yi′brand and Methyl eugenol during entire trial period.Because of the shortest in function time, the quantity of attracted B.dorsalis by zhenfengkexing sticky-trap was the lowest one with no significant difference to the blank control.The results suggest that, by quick function, Haotianyuan′ could be used while the population of B.dorsalis reached in hazard peak to reduce the density of male adult quickly; Yi′brand and Methyl eugenol could be used in monitoring and long-term prevention for controlling of B.dorsalis.
The comparison in field trapping efficacy on B.dorsalis was carried out for zhenfengkexing sticky-trap and 3attractants such as Yi′brand Herbal aromatic attractant (Yi′brand) , Haotianyuan′physical induced adhesive (Haotianyuan′) and Methyl eugenol, in red meat honey pomelo orchard.The results showed that the numbers of B. dorsalis attracted by Haotianyuan′was maximal in overall trial (30d) and single-day for (114.8±27.7) and (15.0 ±3.9) head·bottler-1, respectively, which were significantly higher than those of‘Yi'brand, Methyl eugenol and Zhenfengkexing sticky-trap.The differences in field trapping efficacy of 4attracts were remarkable for various trapping time schedule as showed in following: (1) the highest trapping number of B.dorsalis was obtained by Haotianyuan′in two time phases of the 1-5dand 6-10dafter treatment; (2) during the 20-25dafter treatment, the trapping number was significantly lower in Haotianyuan′than in Yi′brand; (3) there was no significant difference in attracting quantity between Yi′brand and Methyl eugenol during entire trial period.Because of the shortest in function time, the quantity of attracted B.dorsalis by zhenfengkexing sticky-trap was the lowest one with no significant difference to the blank control.The results suggest that, by quick function, Haotianyuan′ could be used while the population of B.dorsalis reached in hazard peak to reduce the density of male adult quickly; Yi′brand and Methyl eugenol could be used in monitoring and long-term prevention for controlling of B.dorsalis.
2013, 28(12): 1277-1280.
doi: 10.19303/j.issn.1008-0384.2013.12.018
Abstract:
The toxicity of 10pesticides and field control efficacy of 4systemic pesticides to Diaphorina citri were tested with dipping and root pouring respectively for effectively controlling the citrus psylla.The result of indoor determination showed that, after 24htreatment, the toxicity of pesticides against Diaphorina citri in LC50 of lambda-cyhalothrin and decamethrin was 2.116and 3.030 mg·L-1, respectively, which were higher than it of Pymetrozine, spirotetramat and Imidacloprid.The lowest toxicity was pymetrozine with LC50 2 025.489mg·L-1. Though the effect of 4systemic pesticides controlling citrus psylla in the field was low in short term with root pouring treatment, the control effects of 10% nitenpyram AS 2 000times、70%imidacloprid WG 14 000times and 7 000times were maintained at 87%during the 14-28dof the treatment and even reached higher than 97%28days after treatments.
The toxicity of 10pesticides and field control efficacy of 4systemic pesticides to Diaphorina citri were tested with dipping and root pouring respectively for effectively controlling the citrus psylla.The result of indoor determination showed that, after 24htreatment, the toxicity of pesticides against Diaphorina citri in LC50 of lambda-cyhalothrin and decamethrin was 2.116and 3.030 mg·L-1, respectively, which were higher than it of Pymetrozine, spirotetramat and Imidacloprid.The lowest toxicity was pymetrozine with LC50 2 025.489mg·L-1. Though the effect of 4systemic pesticides controlling citrus psylla in the field was low in short term with root pouring treatment, the control effects of 10% nitenpyram AS 2 000times、70%imidacloprid WG 14 000times and 7 000times were maintained at 87%during the 14-28dof the treatment and even reached higher than 97%28days after treatments.
2013, 28(12): 1281-1284.
doi: 10.19303/j.issn.1008-0384.2013.12.019
Abstract:
The effect of shading in tea garden on population dynamics of small green leafhopper (SGL) was investigated for their eggs, nymphae and adults with plant-flapping and microscopic examination.The results showed that the effect of shading in tea garden was not significant in the population of SGL adults (F=0.836, P> 0.05) but significant on their nymph (F=8.04, P<0.01) and egg density (F=7.183, P<0.05) .The shading in tea garden might delay the impairing of small green leafhopper migration without changing on their occurrence regulation.The population dynamics of small green leafhopper had two peaks annually.The population of SGL adults was significantly positive correlation to its populations in the nymphae and eggs.
The effect of shading in tea garden on population dynamics of small green leafhopper (SGL) was investigated for their eggs, nymphae and adults with plant-flapping and microscopic examination.The results showed that the effect of shading in tea garden was not significant in the population of SGL adults (F=0.836, P> 0.05) but significant on their nymph (F=8.04, P<0.01) and egg density (F=7.183, P<0.05) .The shading in tea garden might delay the impairing of small green leafhopper migration without changing on their occurrence regulation.The population dynamics of small green leafhopper had two peaks annually.The population of SGL adults was significantly positive correlation to its populations in the nymphae and eggs.
2013, 28(12): 1285-1290.
doi: 10.19303/j.issn.1008-0384.2013.12.020
Abstract:
Five leguminous green manure plants, Indigofera endecaphyllajacq., Cassia sophera L., Chamaecrista nictitans, Chamaecrista rotundifolia CPI86134and Chamaecrista rotundifolia cv Wynn CPI34721, were used as mulching materials to study on the effects of inter-row leguminous grass mulching on soil physical and chemical properties of young tea plantation, with no covering as the control.The results showed that the soil physical characteristics were improved by increasing capillary porosity, field moisture capacity, saturated water content and the rate of liquid soil after continuous 3years mulch.Contents of soil organic matter, total nitrogen, available nitrogen and phosphorus were increased by 9.82%-16.91%, 5.29-14.31%, 2.71%-13.07%, 0.71%-33.98%, respectively.The available potassium contents and pH values varied among different kinds of grasses. Mulching of erect Chamaecrista nictitans, erect Cassia sophera L.and semi-erect Chamaecrista rotundifolia CPI86134inhibited tea growth to a certain degree, but creeping Indigofera endecaphylla jacq.and Chamaecrista rotundifoliacv Wynn CPI34721promoted tea growth with no significant difference.Indigofera endecaphyllajacq. and Chamaecrista rotundifolia cv Wynn CPI34721were proved suitable for mulching on young tea plantation based on the comprehensive results of soil physical and chemical characteristics, soil fertility and tea growth.
Five leguminous green manure plants, Indigofera endecaphyllajacq., Cassia sophera L., Chamaecrista nictitans, Chamaecrista rotundifolia CPI86134and Chamaecrista rotundifolia cv Wynn CPI34721, were used as mulching materials to study on the effects of inter-row leguminous grass mulching on soil physical and chemical properties of young tea plantation, with no covering as the control.The results showed that the soil physical characteristics were improved by increasing capillary porosity, field moisture capacity, saturated water content and the rate of liquid soil after continuous 3years mulch.Contents of soil organic matter, total nitrogen, available nitrogen and phosphorus were increased by 9.82%-16.91%, 5.29-14.31%, 2.71%-13.07%, 0.71%-33.98%, respectively.The available potassium contents and pH values varied among different kinds of grasses. Mulching of erect Chamaecrista nictitans, erect Cassia sophera L.and semi-erect Chamaecrista rotundifolia CPI86134inhibited tea growth to a certain degree, but creeping Indigofera endecaphylla jacq.and Chamaecrista rotundifoliacv Wynn CPI34721promoted tea growth with no significant difference.Indigofera endecaphyllajacq. and Chamaecrista rotundifolia cv Wynn CPI34721were proved suitable for mulching on young tea plantation based on the comprehensive results of soil physical and chemical characteristics, soil fertility and tea growth.
2013, 28(12): 1291-1297.
doi: 10.19303/j.issn.1008-0384.2013.12.021
Abstract:
A laboratory incubation test was conducted to study the emission potential of greenhouse gases from tea garden soils and its responses to the soil nitrogen fertilizer.Four soil types (red earth, moisture sandy soil, alpine meadow soil and purple soil) were collected from tea gardens in Wuyishan City.The results showed that the greenhouse gas emission potential was significantly different between soil types (P<0.05) , the emission from alpine meadow soil was the largest.Nitrogen fertilizer increased the emission of greenhouse gases differently in cumulative amount of CO2, CH4 and N2 O for 21.27%-85.23%, 2.34%-38.58% and 21.27%-85.23%, respectively.The emission of N2 O responded more sensitively to nitrogen fertilizer than it of CO2.The application of nitrogen fertilizer significantly increased the emission of greenhouse gases from the alpine meadow soil and moisture sandy soil.The amount of CO2 dominated in the greenhouse gases of Tea garden soil, which contributed for 94% of greenhouse emission from Tea garden approximately, and it of N2 O contributed approximately from 1.44%to 5.89%, but the CH4 emission was low in Tea garden soil.The greenhouse gas emission from Tea garden was significantly correlative to the content of soil organic carbon, total N, soil NO-3-N, soil NH+ 4-N, soil bulk density and soil porosity, but not to total P, C/N and pH value.
A laboratory incubation test was conducted to study the emission potential of greenhouse gases from tea garden soils and its responses to the soil nitrogen fertilizer.Four soil types (red earth, moisture sandy soil, alpine meadow soil and purple soil) were collected from tea gardens in Wuyishan City.The results showed that the greenhouse gas emission potential was significantly different between soil types (P<0.05) , the emission from alpine meadow soil was the largest.Nitrogen fertilizer increased the emission of greenhouse gases differently in cumulative amount of CO2, CH4 and N2 O for 21.27%-85.23%, 2.34%-38.58% and 21.27%-85.23%, respectively.The emission of N2 O responded more sensitively to nitrogen fertilizer than it of CO2.The application of nitrogen fertilizer significantly increased the emission of greenhouse gases from the alpine meadow soil and moisture sandy soil.The amount of CO2 dominated in the greenhouse gases of Tea garden soil, which contributed for 94% of greenhouse emission from Tea garden approximately, and it of N2 O contributed approximately from 1.44%to 5.89%, but the CH4 emission was low in Tea garden soil.The greenhouse gas emission from Tea garden was significantly correlative to the content of soil organic carbon, total N, soil NO-3-N, soil NH+ 4-N, soil bulk density and soil porosity, but not to total P, C/N and pH value.
2013, 28(12): 1298-1302.
doi: 10.19303/j.issn.1008-0384.2013.12.022
Abstract:
Standardization has become inescapable for agriculture industrialization and modernization in a large scale, and tea standardization, as a main trend for our national tea industry development, has played a significant role in improving tea quality, competitiveness and modernization and so on.In this article, on the basis of the concept and significance of tea standardization, the status and problems of tea standardization development in Fujian province are analyzed, with ways and suggestions to promote tea standardization in Fujian province.
Standardization has become inescapable for agriculture industrialization and modernization in a large scale, and tea standardization, as a main trend for our national tea industry development, has played a significant role in improving tea quality, competitiveness and modernization and so on.In this article, on the basis of the concept and significance of tea standardization, the status and problems of tea standardization development in Fujian province are analyzed, with ways and suggestions to promote tea standardization in Fujian province.
2013, 28(12): 1303-1309.
doi: 10.19303/j.issn.1008-0384.2013.12.023
Abstract:
This paper summarized the achievements in germplasm resources research and breeding operation of abalone in recent years.The germplasm resources research included the resources and distribution of abalone in china, chromosome analysis, abalone population genetic structure and genetic background analysis.Among them, population genetic structure and genetic background analysis included the research of biochemical markers, DNA molecular markers and genetic linkage maps constructions.Breeding operation research included:the introduction of abalone in china, the hybridization and selective breeding operation, modern biological technology (polyploid, gynogenesis, transgenic) in the application of abalone breeding.The further research directions in genetic and breeding of abalone were also proposed at the end of this paper.
This paper summarized the achievements in germplasm resources research and breeding operation of abalone in recent years.The germplasm resources research included the resources and distribution of abalone in china, chromosome analysis, abalone population genetic structure and genetic background analysis.Among them, population genetic structure and genetic background analysis included the research of biochemical markers, DNA molecular markers and genetic linkage maps constructions.Breeding operation research included:the introduction of abalone in china, the hybridization and selective breeding operation, modern biological technology (polyploid, gynogenesis, transgenic) in the application of abalone breeding.The further research directions in genetic and breeding of abalone were also proposed at the end of this paper.
2013, 28(12): 1310-1315.
doi: 10.19303/j.issn.1008-0384.2013.12.024
Abstract:
Recent years, the rapid growth of tea demand further promote the rapid development of tea industry.The solids waste which produced by tea cultivation and processing is also increasing.This not only causes the tea industry tremendous waste of resources, but also brings some pressure and burden to agricultural ecological environment.Therefore, how to utilize the solids waste becomes to be addressed, or not to be ignored significant research study in tea industry and environment area in future.Then, This paper reviews the latest research progress of the solid wastes resource in tea industry, exploring the status of the solid waste reuse and focusing on the application in the environmental field.Aiming to effectively solve the environmental pollution caused by the numerous solid wastes and provide a theoretical basis of the solid wastes reuse in tea industry.
Recent years, the rapid growth of tea demand further promote the rapid development of tea industry.The solids waste which produced by tea cultivation and processing is also increasing.This not only causes the tea industry tremendous waste of resources, but also brings some pressure and burden to agricultural ecological environment.Therefore, how to utilize the solids waste becomes to be addressed, or not to be ignored significant research study in tea industry and environment area in future.Then, This paper reviews the latest research progress of the solid wastes resource in tea industry, exploring the status of the solid waste reuse and focusing on the application in the environmental field.Aiming to effectively solve the environmental pollution caused by the numerous solid wastes and provide a theoretical basis of the solid wastes reuse in tea industry.
2013, 28(12): 1316-1318.
doi: 10.19303/j.issn.1008-0384.2013.12.025
Abstract:
The pollen of four pomelo cultivars (Wendan, Pingshan, Guanxi and Hongrou) was observed with scanning electron microscopy for comparing their pollen morphology, exine ornamentation and germinal aperture. The results showed that the pollen shape of four pomelo cultivars was all in sphere, and the size was 23.40-25.82 μm ×26.05-28.00μm, the exine pattern was fine reticulation and the germinal aperture was all in colporate.The pollen of‘guanximiyou'and‘hongroumiyou'were similar in morphology, the murus of exine was smoother, the mesh was shallow and with 4or 5of colporates (while wendanyou′and pingshanyou′only 4) .The data of the pollen morphology could be used further in the identification and systematic classification for pomelo varieties.
The pollen of four pomelo cultivars (Wendan, Pingshan, Guanxi and Hongrou) was observed with scanning electron microscopy for comparing their pollen morphology, exine ornamentation and germinal aperture. The results showed that the pollen shape of four pomelo cultivars was all in sphere, and the size was 23.40-25.82 μm ×26.05-28.00μm, the exine pattern was fine reticulation and the germinal aperture was all in colporate.The pollen of‘guanximiyou'and‘hongroumiyou'were similar in morphology, the murus of exine was smoother, the mesh was shallow and with 4or 5of colporates (while wendanyou′and pingshanyou′only 4) .The data of the pollen morphology could be used further in the identification and systematic classification for pomelo varieties.
