Transcriptome Analysis on Pathway of and Genes Related to Flavonoid Synthesis in <i>Dendrobium officinale</i>

LIN Jiang-bo; WANG Wei-ying; ZOU Hui; DAI Yi-min

doi:10.19303/j.issn.1008-0384.2019.09.004

Volume 34 Issue 9

Sep. 2019

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Article Navigation > Fujian Journal of Agricultural Sciences > 2019 > 34(9): 1019-1025

LIN J B, WANG W Y, ZOU H, et al. Transcriptome Analysis on Pathway of and Genes Related to Flavonoid Synthesis in Dendrobium officinale [J]. Fujian Journal of Agricultural Sciences，2019，34（9）：1019−1025. doi: 10.19303/j.issn.1008-0384.2019.09.004

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LIN J B, WANG W Y, ZOU H, et al. Transcriptome Analysis on Pathway of and Genes Related to Flavonoid Synthesis in Dendrobium officinale [J]. Fujian Journal of Agricultural Sciences，2019，34（9）：1019−1025. doi: 10.19303/j.issn.1008-0384.2019.09.004

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Transcriptome Analysis on Pathway of and Genes Related to Flavonoid Synthesis in Dendrobium officinale

doi: 10.19303/j.issn.1008-0384.2019.09.004

Subtropical Agriculture Research Institute , Fujian Academy of Agricultural Sciences, Zhangzhou, Fujian　363005, China

Received Date: 2019-07-17
Rev Recd Date: 2019-08-05
Publish Date: 2019-09-01

Abstract

Abstract

Objective Pathway and genes related to the flavonoid synthesis in Dendrobium officinale were analyzed for studying on the metabolic regulation and medicinal applications of flavonoids. Method Transcriptomes of the stems and leaves of D. officinale plants at two growth stages were determined by means of Illumina HiSeq 4000 high-throughput sequencing. The assembled unigenes were used for the functional annotation and genetic analysis of the flavonoid biosynthetic pathway. Result There were 48 unigenes involving 14 enzymes found to be associated with the flavonoid metabolism. Among them, the 5 CHS-related unigenes showed a CHS-like conserved domain, highly conserved amino acid residues (Cys-His-Asn) at the active site, and mutated amino acid residues at the malonyl-CoA and product binding sites; and, the CHI (Unigene0013781) was identified as a Type I chalcone isomerase, isomerizing 6'-hydroxychalone to 5-hydroxyflavanone. The expressions of CHS (Unigene0008250), CHI (Unigene0013781) and F3H were higher than those of CHS (Unigene 0012884) and C3′H in the stems and leaves of the plants at both growth stages. Conclusion Forty-eight unigenes involving 14 enzymes associated with the flavonoid metabolism in D. officinale plants were identified by the transcriptome analysis. Of the 5 CHS-related unigenes, two encoded chalcone synthase, and 3 bibenzyl synthase, while the CHI (Unigene0013781) a Type I chalcone isomerase.
- Dendrobium officinale,
- transcriptome,
- flavonoids,
- function annotation,
- expression,
- metabolism

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References(29)

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