Transferability and Polymorphism of <i>Cymbidium</i> Hybrid EST-SSR Markers in Chinese<i> Cymbidium</i> Cultivars

LIN Rongyan; CHEN Yiquan; ZHONG Huaiqin; WU Jianshe; LIN Bing

doi:10.19303/j.issn.1008-0384.2020.05.006

Volume 35 Issue 5

May 2020

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Article Navigation > Fujian Journal of Agricultural Sciences > 2020 > 35(5): 503-508

LIN R Y, CHEN Y Q, ZHONG H Q, et al. Transferability and Polymorphism of Cymbidium Hybrid EST-SSR Markers in Chinese Cymbidium Cultivars [J]. Fujian Journal of Agricultural Sciences，2020，35（5）：503−508 doi: 10.19303/j.issn.1008-0384.2020.05.006

Citation:

LIN R Y, CHEN Y Q, ZHONG H Q, et al. Transferability and Polymorphism of Cymbidium Hybrid EST-SSR Markers in Chinese Cymbidium Cultivars [J]. Fujian Journal of Agricultural Sciences，2020，35（5）：503−508 doi: 10.19303/j.issn.1008-0384.2020.05.006

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Transferability and Polymorphism of Cymbidium Hybrid EST-SSR Markers in Chinese Cymbidium Cultivars

doi: 10.19303/j.issn.1008-0384.2020.05.006

1.
Institute of Crop Sciences, Fujian Academy of Agricultural Sciences/Flower Research Center, Fujian Academy of Agricultural Science/Fujian Engineering Research Center for Characteristic Floriculture, Fuzhou, Fujian　350013, China
2.
Agricultural Engineering and Technology Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350003, China

Received Date: 2019-12-30
Rev Recd Date: 2020-03-03
Publish Date: 2020-05-01

Abstract

Abstract

Objective Cymbidium hybrid EST-SSR markers were studied to determine their transferability for applications on the cultivar identification and germplasm utilization of Chinese cymbidium cultivars. Method Genetic diversity of 240 pairs EST-SSR primers in 44 cultivars of Cymbidium goeringii, C. ensifolium, C. sinense and others were analyzed, and their transferability among Chinese cymbidium cultivars examined. Result The rates of transferability were found to be 67.92% for C. goeringii, 66.25% for C. ensifolium, and 71.25% for C. sinense. Among the markers, 17 EST-SSR primer pairs were selected to study the molecular phylogeny of the 44 cultivars. Out of them, 10 primer pairs showed polymorphisms with expanded 52 bands. The bands numbered between 3 to 9 with a range of polymorphism information content between 0.504 and 0.850. These cultivars were clustered into 4 groups, same as concluded by the conventional botanical classification. Conclusion The Cymbidium hybrid EST-SSR markers selected from this study effectively showed the molecular genetic diversity of the various Chinese cymbidium cultivars.
- Cymbidium hybrid,
- EST-SSR marker,
- Chinese cymbidium,
- transferability,
- polymorphism

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References(20)

References

[1]	龚理, 黄添毅, 王芳, 等. 国兰杂交品种(系)SRAP-PCR反应体系优化及引物快速筛选 [J]. 热带作物学报, 2014, 35(5):925−932. doi: 10.3969/j.issn.1000-2561.2014.05.016 GONG L, HUANG T Y, WANG F, et al. Optimization SRAP-PCR system and quickly screening primers for hybridization cultivars(lines) of Chinese Cymbidium [J]. Chinese Journal of Tropical Crops, 2014, 35(5): 925−932.(in Chinese) doi: 10.3969/j.issn.1000-2561.2014.05.016
[2]	陈和明, 朱根发, 廖飞雄, 等. 广东省兰花种质资源的收集保存与评价利用 [J]. 广东农业科学, 2007, 34(6):27−29. doi: 10.3969/j.issn.1004-874X.2007.06.010 CHEN H M, ZHU G F, LIAO F X, et al. Collection, conservation, evaluation and utilization of Orchidaceae germplasm in Guangdong [J]. Guangdong Agricultural Sciences, 2007, 34(6): 27−29.(in Chinese) doi: 10.3969/j.issn.1004-874X.2007.06.010
[3]	唐源江, 曹雯静, 吴坤林. 基于SRAP标记的国兰种质资源遗传多样性分析及分子身份证构建 [J]. 中国农业科学, 2015, 48(9):1795−1806. doi: 10.3864/j.issn.0578-1752.2015.09.13 TANG Y J, CAO W J, WU K L. Genetic diversity analysis and molecular identification card construction of Chinese Cymbidium germplasms based on SRAP markers [J]. Scientia Agricultura Sinica, 2015, 48(9): 1795−1806.(in Chinese) doi: 10.3864/j.issn.0578-1752.2015.09.13
[4]	江亚雯, 孙小琴, 罗火林, 等. 基于ISSR标记的江西野生寒兰居群遗传多样性研究 [J]. 园艺学报, 2017, 44(10):1993−2000. JIANG Y W, SUN X Q, LUO H L, et al. Studies on genetic diversity of Cymbidium kanran populations from the main mountains in Jiangxi Province based on ISSR marker [J]. Acta Horticulturae Sinica, 2017, 44(10): 1993−2000.(in Chinese)
[5]	王晓英, 张林, 李承秀, 等. 51个春兰(Cymbidium goeringii)品种的AFLP遗传多样性分析 [J]. 植物遗传资源学报, 2015, 16(3):653−658. WANG X Y, ZHANG L, LI C X, et al. Genetic diversity analysis of 51 Cymbidium goeringii cultivars by AFLP markers [J]. Journal of Plant Genetic Resources, 2015, 16(3): 653−658.(in Chinese)
[6]	胡薇, 黄儒珠, 潘晓华, 等. 建兰38个品种的RAPD分析 [J]. 园艺学报, 2008, 35(2):289−294. doi: 10.3321/j.issn:0513-353X.2008.02.022 HU W, HUANG R Z, PAN X H, et al. RAPD analysis of thirty-eight Cymbidium ensifolium cultivars [J]. Acta Horticulturae Sinica, 2008, 35(2): 289−294.(in Chinese) doi: 10.3321/j.issn:0513-353X.2008.02.022
[7]	ISABEL L P, PARK J R, LEE G S, et al. Development of EST-SSR markers and analysis of genetic relationship it's resources in hexaploid oats [J]. Journal of Crop Science and Biotechnology, 2019, 22(3): 243−251. doi: 10.1007/s12892-019-0158-0
[8]	FERRÃO L F V, CAIXETA E T, PENA G, et al. New EST–SSR markers of Coffea arabica: transferability and application to studies of molecular characterization and genetic mapping [J]. Molecular Breeding, 2015, 35: 31. doi: 10.1007/s11032-015-0247-z
[9]	AWASTHI P, SINGH A, SHEIKH G, et al. Mining and characterization of EST-SSR markers for Zingiber officinale Roscoe with transferability to other species of Zingiberaceae [J]. Physiology and Molecular Biology of Plants, 2017, 23(4): 925−931. doi: 10.1007/s12298-017-0472-5
[10]	LEONARDUZZI C, SPANU I, LABRIOLA M, et al. Development and characterization of three highly informative EST-SSR multiplexes for Pinus halepensis mill. And their transferability to other Mediterranean pines [J]. Plant Molecular Biology Reporter, 2016, 34(5): 993−1002. doi: 10.1007/s11105-016-0980-4
[11]	SAISUG W, UKOSKIT K. Comparative analysis of EST-derived markers for allelic variation in Jatropha curcas L. and cross transferability among economically important species of Euphorbiaceae [J]. Genes & Genomics, 2013, 35(1): 1−12.
[12]	李丽辉, 胡瑶, 李宏告, 等. 基于RAPD、ISSR标记的国兰种质资源遗传多样性研究 [J]. 中国农学通报, 2018, 34(29):42−47. doi: 10.11924/j.issn.1000-6850.casb17100075 LI L H, HU Y, LI H G, et al. Study on genetic diversity of China Orchid resources using RAPD and ISSR markers [J]. Chinese Agricultural Science Bulletin, 2018, 34(29): 42−47.(in Chinese) doi: 10.11924/j.issn.1000-6850.casb17100075
[13]	张亚楠, 杨柏云, 熊冬金, 等. 寒兰转录组SSR信息分析及其分子标记开发 [J]. 南昌大学学报(理科版), 2017, 41(3):249−254. ZHANG Y N, YANG B Y, XIONG D J, et al. Analysis on SSR information based on transcriptome and development of molecular markers in Cymbidium kanran [J]. Journal of Nanchang University(Natural Science), 2017, 41(3): 249−254.(in Chinese)
[14]	牛田, 张林, 王厚新, 等. 利用SRAP标记分析春兰种质资源遗传多样性 [J]. 农学学报, 2014, 4(8):53−58. NIU T, ZHANG L, WANG H X, et al. Genetic diversity analysis of Cymbidium goeringii's germplasm resources basedon SRAP markers [J]. Journal of Agriculture, 2014, 4(8): 53−58.(in Chinese)
[15]	郑永胜, 张晗, 王雪梅, 等. 小麦DUS测试已知品种DNA指纹数据库构建及其应用 [J]. 植物遗传资源学报, 2019, 20(4):845−853. ZHENG Y S, ZHANG H, WANG X M, et al. Construction of DNA profile database of Wheat reference varieties and its application in Wheat DUS Test [J]. Journal of Plant Genetic Resources, 2019, 20(4): 845−853.(in Chinese)
[16]	刘栓桃, 张志刚, 王荣花, 等. 基于InDel标记的大白菜育种材料分子身份证构建 [J]. 中国蔬菜, 2019(2):34−41. LIU S T, ZHANG Z G, WANG R H, et al. Construction of molecular identity card for Chinese cabbage breeding materials based on InDel markers [J]. China Vegetables, 2019(2): 34−41.(in Chinese)
[17]	蔡年辉, 许玉兰, 王大玮, 等. 思茅松EST-SSR标记在几种松属植物中的通用性分析 [J]. 西南林业大学学报(自然科学), 2017, 37(4):8−14. CAI N H, XU Y L, WANG D W, et al. The transferability analysis of Pinus kesiya var. langbianensis EST-SSR markers to several pine species [J]. Journal of Southwest Forestry University(Natural Sciences), 2017, 37(4): 8−14.(in Chinese)
[18]	陈金金, 彭俊华. 小麦EST-SSR标记对几种有潜力的禾本科能源植物的可转移性研究 [J]. 植物科学学报, 2011, 29(6):696−703. CHEN J J, PENG J H. Transferability of wheat(Triticum aestivum) EST-derived SSR markers to several potential energy plants in Gramineae [J]. Plant Science Journal, 2011, 29(6): 696−703.(in Chinese)
[19]	文明富, 陈新, 王海燕, 等. 木薯基因组SSR和EST-SSR在麻疯树和橡胶树中的通用性分析 [J]. 作物学报, 2011, 37(1):74−78. doi: 10.3724/SP.J.1006.2011.00074 WEN M F, CHEN X, WANG H Y, et al. Transferability analysis of cassava EST-SSR and genomic-SSR markers in Jatropha and rubber tree [J]. Acta Agronomica Sinica, 2011, 37(1): 74−78.(in Chinese) doi: 10.3724/SP.J.1006.2011.00074
[20]	蒋彧, 何俊蓉, 刘菲, 等. 中国兰遗传多样性的SRAP分析 [J]. 西南农业学报, 2013, 26(4):1645−1648. doi: 10.3969/j.issn.1001-4829.2013.04.070 JIANG Y, HE J R, LIU F, et al. Genetic polymorphism analysis by sequence-related amplified polymorphism in Chinese Cymbidium [J]. Southwest China Journal of Agricultural Sciences, 2013, 26(4): 1645−1648.(in Chinese) doi: 10.3969/j.issn.1001-4829.2013.04.070