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Volume 35 Issue 7
Jul.  2020
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Article Contents
CHEN R J, WU X M, CHEN Q Y, et al. A TaqMan RT-PCR Method for Detecting Porcine Circovirus 3 [J]. Fujian Journal of Agricultural Sciences,2020,35(7):739−745 doi: 10.19303/j.issn.1008-0384.2020.07.007
Citation: CHEN R J, WU X M, CHEN Q Y, et al. A TaqMan RT-PCR Method for Detecting Porcine Circovirus 3 [J]. Fujian Journal of Agricultural Sciences,2020,35(7):739−745 doi: 10.19303/j.issn.1008-0384.2020.07.007

A TaqMan RT-PCR Method for Detecting Porcine Circovirus 3

doi: 10.19303/j.issn.1008-0384.2020.07.007
  • Received Date: 2019-09-23
  • Rev Recd Date: 2020-05-05
  • Publish Date: 2020-07-31
  •   Objective  A TaqMan RT-PCR method was established for detecting porcine circovirus 3 (PCV3) infection in swine.   Method  Specific primers and probe were designed by Oligo 7 software targeting the Rep gene after genetic comparison.   Result  The established TaqMan RT-PCR method could detect 42.2 copies·uL−1 with no positive signal on common porcine infectious diseases indicating high specificity of the methodology. On the constructed positive plasmids, the coefficients of variation for the intra- and inter-assays were less than 1.48% showing a high detection reproducibility. From the 193 clinical specimens collected in Fujian from 2014 to 2018, the newly developed method showed a high prevalence of PCV3 at 65.80% and high co-infection with PCV2 at 52.85%.   Conclusion  The established TaqMan RT-PCR method was made available for studying the function of Rep in PCV3.
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