Optimization of Media for Seed Germination and Propagation of <i>Arundina graminifolia</i>

XIA Chunying; XIE Xiaomin; LIU Jiangfeng; ZHENG Chengle

doi:10.19303/j.issn.1008-0384.2020.11.006

Volume 35 Issue 11

Nov. 2020

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Article Navigation > Fujian Journal of Agricultural Sciences > 2020 > 35(11): 1215-1221

XIA C Y, XIE X M, LIU J F, et al. Optimization of Media for Seed Germination and Propagation of Arundina graminifolia [J]. Fujian Journal of Agricultural Sciences，2020，35（11）：1215−1221 doi: 10.19303/j.issn.1008-0384.2020.11.006

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XIA C Y, XIE X M, LIU J F, et al. Optimization of Media for Seed Germination and Propagation of Arundina graminifolia [J]. Fujian Journal of Agricultural Sciences，2020，35（11）：1215−1221 doi: 10.19303/j.issn.1008-0384.2020.11.006

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Optimization of Media for Seed Germination and Propagation of Arundina graminifolia

doi: 10.19303/j.issn.1008-0384.2020.11.006

1.
College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China
2.
Management Office of Yu Shan Scenic Area, Fuzhou, Fujian　350001, China

Received Date: 2020-04-18
Rev Recd Date: 2020-08-06

Available Online: 2020-11-24

Publish Date: 2020-11-30

Abstract

Abstract

Objective Formulation of culture media was optimized to facilitate the seed germination, tissue propagation, and various developmental stages of Arundina graminifolia. Method An orthogonal experiment was conducted to optimize the media for aseptic germination of the seeds and development of the seedlings of A. graminifolia using 1/2 MS as the base. Application of growth regulators and additives in the solid medium formulation was evaluated in accordance to the seed germination, seedling growth, root development and transplanting efficiency. Result The optimum media for various developmental stages of A. graminifolia were determined to include (1) 1/2 MS + 7 g agar·L⁻¹ + 30.0 g sucrose·L⁻¹ + 1 g activated carbon·L⁻¹ + 100 mL coconut milk·L⁻¹ + 1.0 mg NAA·L⁻¹ + 2.0 mg 6-BA·L⁻¹ + 0.5g Huabao No. 1·L⁻¹ to reach a seed germination rate of 83.79% . (2) 1/2 MS + 7 g agar·L⁻¹ + 3 0g sucrose·L⁻¹ + 1 g activated carbon·L⁻¹ + 1 mg IBA·L⁻¹ + 3 mg 6-BA·L⁻¹ + 0.5 mg NAA·L⁻¹ + 100 g mashed potato·L⁻¹ to achieve an average bud proliferation rate of 1.36. (3) 1/2 MS + 7 g agar·L⁻¹ + 30 g sucrose·L⁻¹ + 1 g activated carbon·L⁻¹ + 0.2 mg IBA·L⁻¹ + 2 mg 6-BA·L⁻¹ + 0.2-0.5 mg NAA·L⁻¹ + 100 g banana puree·L⁻¹ for an optimal root development. (4) Perlite:peat soil mixture at 1 ∶ 1 ratio to yield a seedling survival rate of 98% with desirable transplanting and acclimatization results. Conclusion Coconut milk in culture medium was found to significantly benefit the seed germination. The individually formulated media for various growth stages of A. graminifolia seedlings would help ensure the success in preserving the wild germplasms.
- Arundina graminifolia (D. Don) Hochr,
- aseptic germination,
- acclimatization and transplant,
- orthogonal design,
- tissue culture

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