Response Surface Optimization of Sialic Acid Extraction from Chicken Small-intestine Mucosa

HU Yueming; SUN Guiju; YAO Hongliang

doi:10.19303/j.issn.1008-0384.2020.06.013

Volume 35 Issue 6

Jun. 2020

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Article Navigation > Fujian Journal of Agricultural Sciences > 2020 > 35(6): 665-672

HU Y M, SUN G J, YAO H L. Response Surface Optimization of Sialic Acid Extraction from Chicken Small-intestine Mucosa [J]. Fujian Journal of Agricultural Sciences，2020，35（6）：665−672 doi: 10.19303/j.issn.1008-0384.2020.06.013

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HU Y M, SUN G J, YAO H L. Response Surface Optimization of Sialic Acid Extraction from Chicken Small-intestine Mucosa [J]. Fujian Journal of Agricultural Sciences，2020，35（6）：665−672 doi: 10.19303/j.issn.1008-0384.2020.06.013

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Response Surface Optimization of Sialic Acid Extraction from Chicken Small-intestine Mucosa

doi: 10.19303/j.issn.1008-0384.2020.06.013

HU Yueming^1
,,
SUN Guiju^{1
,
,},
YAO Hongliang^{2, 3
,
,}

1.
Department of Nutrition and Food Hygiene/Key Laboratory of Environmental Medicine Engineering of Ministry of Education, School of Public Health, Southeast University, Nanjing, Jiangsu　210009, China
2.
Food Science Department, Jinling Institute of Technology, Nanjing, Jiangsu　210038, China
3.
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu　210095, China

Received Date: 2019-11-22
Rev Recd Date: 2020-04-17
Publish Date: 2020-08-10

Abstract

Abstract

Objective A process of extracting sialic acid from chicken small-intestine mucosa was optimized for the utilization of a broiler processing byproduct. Method A single factor experiment was conducted to select appropriate acid for the extraction. Then, a 3-factors Box-Behnken test was designed to optimize the acid concentration, time, and liquid-to-material ratio in extracting sialic acid from chicken intestinal mucosa. Result Seventeen experiments were performed in triplicate to arrive at the optimized process that utilized citric acid at a concentration of 0.1 mol·L⁻¹ in a liquid-to-material ratio of 32.20 ∶ 1 (mL·g⁻¹), which was simplified to 30 ∶ 1 (mL·g⁻¹), to extract for 4 h. The resulting extract contained sialic acid (1 010.39±21.87) μg·g⁻¹, which was close to the targeted goal of 1 025.69 μg·g⁻¹. Conclusion The optimized sialic acid extraction from chicken small-intestine mucosa provided a new venue for byproduct utilization and waste reduction in poultry processing.
- Response surface method,
- chicken small-intestine mucosa,
- sialic acid

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References(23)

References

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