Identification and Expressions under Low-temp/Draught Stress of Polyamine Synthesis Genes in Oil Palms

JIN Longfei; YIN Xinxing; ZHANG Anni; CAO Hongxing

doi:10.19303/j.issn.1008-0384.2021.04.004

Volume 36 Issue 4

Apr. 2021

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2021 > 36(4): 402-411

JIN L F, YIN X X, ZHANG A N, et al. Identification and Expressions under Low-temp/Draught Stress of Polyamine Synthesis Genes in Oil Palms [J]. Fujian Journal of Agricultural Sciences，2021，36（4）：402−412 doi: 10.19303/j.issn.1008-0384.2021.04.004

Citation:

JIN L F, YIN X X, ZHANG A N, et al. Identification and Expressions under Low-temp/Draught Stress of Polyamine Synthesis Genes in Oil Palms [J]. Fujian Journal of Agricultural Sciences，2021，36（4）：402−412 doi: 10.19303/j.issn.1008-0384.2021.04.004

Citation:

PDF( 1239 KB)

Identification and Expressions under Low-temp/Draught Stress of Polyamine Synthesis Genes in Oil Palms

doi: 10.19303/j.issn.1008-0384.2021.04.004

Coconut Research Institute, Chinese Academy of Tropical Agricultural Science/Hainan Key Laboratory of Tropical Oil Crops Biology, Wenchang, Hainan　571339, China

Received Date: 2020-11-02
Rev Recd Date: 2021-01-31

Available Online: 2021-03-27

Publish Date: 2021-04-30

Abstract

Abstract

Objective Genetic information and resource of low-temp/draught resistant oil palms were studied with respect to the stress responses of polyamines synthase gene in the plants for breeding purposes. Method Bioinformatics of polyamines synthase genes in oil palm were gathered from the genome bank, and their expressions under abiotic stresses analyzed. Result Fifteen polyamines synthase genes were identified from the oil palm genome. There were between 318 and 720 amino acids in the genes with a molecular weight ranging from 36.00 kDa to 46.43 kDa and an isoelectric point between 4.78 and 6.31. The instability indices of the genes were found in between 29.26 and 48.73, the aliphatic indices in between 76.46 and 94.41, the gravy in between −0.315 and 0.082, and the exon number in between 1 and 11. They contained both specific conserved protein motifs and conserved domain. A phylogenetic analysis divided them into 3 families, i.e., EgADCs closely related to rice, EgACLs and EgSPDSs closely related to data palm, and EgSPMSs closely related to maize. Many cis-acting elements that response to phytohormone, stresses, and light were found in the promoters. The expressions of the polyamine synthase genes existed in different tissues of the plant and could be induced by either low temperature or draught stress. Specifically, EgSPDS1, EgSPDS2, EgSPMS1, EgADC1, EgADC2, EgACL5-2, EgACL5-3, EgSAMDC3-1, EgSAMDC4-1, EgSAMDC4-2, and EgACL5-1 were found to be upregulated by the imposed cold stress; while EgSPDS1, EgSPDS2, EgADC1, EgADC2, EgSAMDC4-1 EgSAMDC4-2, and EgACL5-1 by dehydration. Conclusion The polyamine synthase genes were significantly expressed in tissues of the oil palms that could be induced by either low temperature or draught stress. Those responded to the abiotic stresses were identified in this study.
- Oil palm,
- polyamine synthase,
- cold,
- dehydration,
- gene expression

FullText(HTML)

References(33)

References

[1]	雷新涛, 曹红星, 冯美利, 等. 热带木本生物质能源树种——油棕 [J]. 中国农业大学学报, 2012, 17(6):185−190. doi: 10.11841/j.issn.1007-4333.2012.06.023 LEI X T, CAO H X, FENG M L, et al. Oil Palm: a tropical woody tree species as biomass energy [J]. Journal of China Agricultural University, 2012, 17(6): 185−190.(in Chinese) doi: 10.11841/j.issn.1007-4333.2012.06.023
[2]	MAHLIA T M I, ISMAIL N, HOSSAIN N, et al. Palm oil and its wastes as bioenergy sources: a comprehensive review [J]. Environmental Science and Pollution Research International, 2019, 26(15): 14849−14866. doi: 10.1007/s11356-019-04563-x
[3]	ZHOU L, YARRA R, JIN L, et al. Genome-wide identification and expression analysis of MYB gene family in oil palm (Elaeis guineensis Jacq.) under abiotic stress conditions [J]. Environmental and Experimental Botany, 2020, 180: 104245. doi: 10.1016/j.envexpbot.2020.104245
[4]	XIAO Y, ZHOU L, LEI X, et al. Genome-wide identification of WRKY genes and their expression profiles under different abiotic stresses in Elaeis guineensis [J]. PLoS One, 2017, 12(12): e189224.
[5]	MICHAEL A J. Biosynthesis of polyamines and polyamine-containing molecules [J]. The Biochemical Journal, 2016, 473(15): 2315−2329. doi: 10.1042/BCJ20160185
[6]	王广龙, 却枫, 陈伯清, 等. 胡萝卜S-腺苷甲硫氨酸脱羧酶SAMDC基因的克隆及其对非生物胁迫的响应 [J]. 植物生理学报, 2017, 53(3):413−421. WANG G L, QUE F, CHEN B Q, et al. Cloning of S-adenosylmethioine decarboxylase gene SAMDC from Daucus carota and its response to abiotic stresses [J]. Plant Physiology Communications, 2017, 53(3): 413−421.(in Chinese)
[7]	王保全, 张晓娜, 刘继红, 等. 桃树PpADC基因克隆及逆境胁迫表达分析 [J]. 西南师范大学学报(自然科学版), 2020, 45(7):34−41. WANG B Q, ZHANG X N, LIU J H, et al. Cloning and abiotic stress-induced expression of the arginine decarboxylase gene from Prunus persica [J]. Journal of Southwest China Normal University (Natural Science Edition), 2020, 45(7): 34−41.(in Chinese)
[8]	孙培培. 枳精氨酸脱羧酶基因PtADC抗逆功能解析及其调控因子分离与鉴定[D]. 武汉: 华中农业大学, 2014, . SUN P P. Functional charaterization of arginine decarboxylase gene PtADC from Poncirus trifoliata and indentification of the regulators[D]. Wuhan: Huazhong Agricultural University, 2014. (in Chinese).
[9]	杨硕知, 刘球, 吴际友, 等. 外源多胺对红椿S-腺苷甲硫氨酸脱羧酶基因表达的调节作用 [J]. 中南林业科技大学学报, 2019, 39(1):116−123. YANG S Z, LIU Q, WU J Y, et al. Gene cloning and expression analysis of TcSAMDC of Toona ciliata [J]. Journal of Central South University of Forestry & Technology, 2019, 39(1): 116−123.(in Chinese)
[10]	王显瑞, 刘莉莉, 柴晓娇, 等. 外源亚精胺对干旱胁迫下谷子幼苗光合作用及碳水化合物积累的影响 [J]. 作物杂志, 2015(5):100−106. WANG X R, LIU L L, CAI X J, et al. Effects of exogenous spermidine on photosynthesis and carbohydrate accumulation in foxtail millet (Setaria italica) seedlings under drought stress [J]. Crops, 2015(5): 100−106.(in Chinese)
[11]	李丹阳, 闫永庆, 殷媛, 等. 外源Spd和NO对盐胁迫下玉竹脯氨酸代谢途径的影响 [J]. 河南农业科学, 2018, 47(6):111−116. LI D Y, YAN Y Q, YING Y, et al. Effects of spd and NO on proline metabolic pathways of polygonatum odoratum (Mill.) druce under salt stress [J]. Journal of Henan Agricultural Sciences, 2018, 47(6): 111−116.(in Chinese)
[12]	HANFREY C, SOMMER S, MAYER M J, et al. Arabidopsis polyamine biosynthesis: absence of ornithine decarboxylase and the mechanism of arginine decarboxylase activity [J]. The Plant Journal, 2001, 27(6): 551−560. doi: 10.1046/j.1365-313X.2001.01100.x
[13]	SHI H, CHAN Z. Improvement of plant abiotic stress tolerance through modulation of the polyamine pathway [J]. Journal of Integrative Plant Biology, 2014, 56(2): 114−121. doi: 10.1111/jipb.12128
[14]	LIU J H, WANG W, WU H, et al. Polyamines function in stress tolerance: from synthesis to regulation [J]. Frontiers in Plant Science, 2015, 6: 827.
[15]	KOU S, CHEN L, TU W, et al. The arginine decarboxylase gene ADC1, associated to the putrescine pathway, plays an important role in potato cold-acclimated freezing tolerance as revealed by transcriptome and metabolome analyses [J]. The Plant Journal, 2018, 96(6): 1283−1298. doi: 10.1111/tpj.14126
[16]	HIDALGO-CASTELLANOS J, DUQUE A S, BURGUEÑO A, et al. Overexpression of the arginine decarboxylase gene promotes the symbiotic interaction Medicago truncatula-Sinorhizobium meliloti and induces the accumulation of proline and spermine in nodules under salt stress conditions [J]. Journal of Plant Physiology, 2019, 241: 153034. doi: 10.1016/j.jplph.2019.153034
[17]	LIU Z, LIU P, QI D, et al. Enhancement of cold and salt tolerance of Arabidopsis by transgenic expression of the S-adenosylmethionine decarboxylase gene from Leymus chinensis [J]. Journal of Plant Physiology, 2017, 211: 90−99. doi: 10.1016/j.jplph.2016.12.014
[18]	JI M, WANG K, WANG L, et al. Overexpression of a S-adenosylmethionine decarboxylase from sugar beet M14 increased Araidopsis salt tolerance [J]. International Journal of Molecular Sciences, 2019, 20(8).
[19]	QIU Z, YAN S, XIA B, et al. The eggplant transcription factor MYB44 enhances resistance to bacterial wilt by activating the expression of spermidine synthase [J]. Journal of Experimental Botany, 2019, 70(19): 5343−5354. doi: 10.1093/jxb/erz259
[20]	SINGH R, ONGABDULLAH M, LOW E T L, et al. Oil palm genome sequence reveals divergence of interfertile species in old and new worlds [J]. Nature, 2013, 500(7462): 335. doi: 10.1038/nature12309
[21]	吴昊. 柑橘转录因子CsCBF1和PtrNAC72在调控精氨酸脱羧酶基因表达及抗逆中的功能鉴定[D]. 武汉: 华中农业大学 2017. WU H. Functional characterization of Citrus sinensis CBF1 and NAC72 in regulation of arginine decarboxylase gene expression and stress tolerance[D]. Wuhan: Huazhong Agricultural University, 2017. (in Chinese).
[22]	CHEN C, CHEN H, ZHANG Y, et al. TBtools: an integrative toolkit developed for interactive analyses of big biological data [J]. Molecular Plant, 2020, 13(8): 1194−1202. doi: 10.1016/j.molp.2020.06.009
[23]	EDGAR R C, BATZOGLOU S. Multiple sequence alignment [J]. Current Opinion in Structural Biology, 2006, 16(3): 368−373. doi: 10.1016/j.sbi.2006.04.004
[24]	VUOSKU J, MUILU-MÄKELÄ R, AVIA K, et al. Thermospermine synthase (ACL5) and diamine oxidase (DAO) expression is needed for zygotic embryogenesis and vascular development in Scots pine [J]. Frontiers in Plant Science, 2019, 10: 1600. doi: 10.3389/fpls.2019.01600
[25]	刘荣, 黄海, 韩树全, 等. 芒果精氨酸脱羧酶基因MiADC的克隆及表达分析 [J]. 农业科学与技术(英文版), 2018, 19(2):9−16. LIU R, HUANG H, HAN S Q, et al. Cloning and expression analysis of arginine decarboxylase gene MiADC from mango [J]. Agricultural Science & Technology, 2018, 19(2): 9−16.(in Chinese)
[26]	GONG X, DOU F, CHENG X, et al. Genome-wide identification of genes involved in polyamine biosynthesis and the role of exogenous polyamines in Malus hupehensis Rehd. under alkaline stress [J]. Gene, 2018, 669: 52−62. doi: 10.1016/j.gene.2018.05.077
[27]	LIU T, HUANG B, CHEN L, et al. Genome-wide identification, phylogenetic analysis, and expression profiling of polyamine synthesis gene family members in tomato [J]. Gene, 2018, 661: 1−10. doi: 10.1016/j.gene.2018.03.084
[28]	MO H, WANG X, ZHANG Y, et al. Cotton ACAULIS5 is involved in stem elongation and the plant defense response to Verticillium dahliae through thermospermine alteration [J]. Plant cell reports, 2015, 34(11): 1975−1985. doi: 10.1007/s00299-015-1844-3
[29]	TSANIKLIDIS G, KOTSIRAS A, TSAFOUROS A, et al. Spatial and temporal distribution of genes involved in polyamine metabolism during tomato fruit development [J]. Plant Physiology and Biochemistry, 2016, 100: 27−36. doi: 10.1016/j.plaphy.2016.01.001
[30]	WOITTIEZ L S, van WIJK M T, SLINGERLAND M, et al. Yield gaps in oil palm: A quantitative review of contributing factors [J]. European Journal of Agronomy, 2017, 83: 57−77. doi: 10.1016/j.eja.2016.11.002
[31]	CORLEY R H V, TINKER P B. The oil palm[M]. New Jersey: Wiley Blackwell, 2016: 324−327.
[32]	ALCÁZAR R, MARCO F, CUEVAS J C, et al. Involvement of polyamines in plant response to abiotic stress [J]. Biotechnology Letters, 2006, 28(23): 1867−1876. doi: 10.1007/s10529-006-9179-3
[33]	KASUKABE Y, HE L, NADA K, et al. Overexpression of spermidine synthase enhances tolerance to multiple environmental stresses and up-regulates the expression of various stress-regulated genes in transgenic Arabidopsis thaliana [J]. Plant & Cell Physiology, 2004, 45(6): 712−722.