Identification and Biology of Leaf Spot Pathogen on <i>Chimonanthus salicifolius</i>

PENG Chengbin; WANG Zerong; RUAN Junfeng; WEI Rifeng; XUE Lan; CHEN Meixia; LIU Wei

doi:10.19303/j.issn.1008-0384.2021.04.012

Volume 36 Issue 4

Apr. 2021

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2021 > 36(4): 457-463

PENG C B, WANG Z R, RUAN J F, et al. Identification and Biology of Leaf Spot Pathogen on Chimonanthus salicifolius [J]. Fujian Journal of Agricultural Sciences，2021，36（4）：457−463 doi: 10.19303/j.issn.1008-0384.2021.04.012

Citation:

PENG C B, WANG Z R, RUAN J F, et al. Identification and Biology of Leaf Spot Pathogen on Chimonanthus salicifolius [J]. Fujian Journal of Agricultural Sciences，2021，36（4）：457−463 doi: 10.19303/j.issn.1008-0384.2021.04.012

Citation:

PDF( 736 KB)

Identification and Biology of Leaf Spot Pathogen on Chimonanthus salicifolius

doi: 10.19303/j.issn.1008-0384.2021.04.012

PENG Chengbin^{1, 2
,},
WANG Zerong¹,
RUAN Junfeng^{1, 3},
WEI Rifeng²,
XUE Lan⁴,
CHEN Meixia^{1, 3
,
,},
LIU Wei^{1, 3
,
,}

1.
College of Life Sciences, Ningde Normal University, Ningde, Fujian　352100, China
2.
College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China
3.
College of Chemistry and Materials, Ningde Normal University, Ningde, Fujian　352100, China
4.
Fujian Science and Technology Industry-University-Research Cooperation Demonstration Base, Ningde, Fujian　352100, China

Received Date: 2020-11-19
Rev Recd Date: 2021-01-25

Available Online: 2021-03-27

Publish Date: 2021-04-30

Abstract

Abstract

Objective Pathogen that caused the leaf spot disease on Chimonanthus salicifolius was isolated and classified, and its biological characteristics determined. Method Specimens of C. salicifolius plants infected by leaf spot disease at Shouning county in Fujian were collected for in vitro tissue culture in the laboratory. Morphological and molecular examinations were performed on the isolates. Biological properties and culture conditions including temperature, pH, light, carbon source, nitrogen source, and lethal temperature were scrutinized for the pathogenic identification and characterization. Result Based on the morphology as well as the rDNA ITS and TUB sequences, the pathogen responsible for the disease was identified to be Ascomycetes Pleosporales Epicoccum sorghinum. It could grow at 5-30 ℃ with an optimum temperature at 25 ℃ and pH ranging from 5 to 9 in media of 11 various carbon sources including sucrose and 8 different nitrogen source including peptone under no specific light/dark conditions. The lethal temperature of the fungal mycelia was 52 ℃. Conclusion E. sorghinum was identified to be the major pathogen that caused the leaf spot disease on C. salicifolius in Shouning county. It had strong ability to grow on, resist to, and survive under adverse conditions.
- Chimonanthus salicifolius,
- pathogenic identification,
- multiple gene sequence analysis,
- biological characteristics

FullText(HTML)

References(20)

References

[1]	LV Q D, QIU J, LIU J, et al. The Chimonanthus salicifolius genome provides insight into magnoliid evoluDion and flavonoid biosynthesis [J]. The Plant Journal, 2020, 103（5）: 1910-1923. DOI: 10.1111/tpj.14874.
[2]	叶玉娟, 叶龙华. 柳叶蜡梅的研究综述及开发应用前景 [J]. 黄山学院学报, 2015, 17(5):68−71. doi: 10.3969/j.issn.1672-447X.2015.05.018 YE Y J, YE L H. The research summary and application prospect of Chimonanthus salicifolius S. Y. H [J]. Journal of Huangshan University, 2015, 17(5): 68−71.(in Chinese) doi: 10.3969/j.issn.1672-447X.2015.05.018
[3]	郭孝成, 王伟, 戴毅, 等. 柳叶蜡梅不同部位提取物总多酚含量及体外抗氧化、抑菌特性比较研究 [J]. 黄山学院学报, 2018, 20(5):66−70. doi: 10.3969/j.issn.1672-447X.2018.05.015 GUO X C, WANG W, DAI Y, et al. Comparative study of total polyphenol content, antioxidant, antimicrobial activities in the extracts from different parts of Chimonanthus salicifolicus hu [J]. Journal of Huangshan University, 2018, 20(5): 66−70.(in Chinese) doi: 10.3969/j.issn.1672-447X.2018.05.015
[4]	廖庭. 烟草茎点霉叶斑病的病原鉴定及防治药剂筛选[D]. 南宁: 广西大学, 2015. LIAO T. Pathogen identification and fungicides screening of tobacco leaf spot of Phoma sorghina[D]. Naning: Guangxi University, 2015. (in Chinese).
[5]	ZENG H, LU Q, LI R. First report of leaf spot of lily caused by Epicoccum sorghinum in China [J]. Plant Disease, 2018, 102(12): 2648.
[6]	林宇, 李增平, 吴如慧, 等. 非洲楝拟茎点霉叶斑病病原菌鉴定及其生物学特性的测定 [J]. 热带生物学报, 2019, 10(1):34−40. LIN Y, LI Z P, WU R H, et al. Identification and biological characteristics of the pathogen causing Phomopsis leaf spot in Khaya senegalensis [J]. Journal of Tropical Biology, 2019, 10(1): 34−40.(in Chinese)
[7]	窦晓丽, 胡文静, 刘凡, 等. 新疆2种苜蓿叶斑病病原菌主要生物学特性 [J]. 新疆农业科学, 2020, 57(10):1863−1870. DOU X L, HU W J, LIU F, et al. Biological characteristics of pathogens causing of two Alfalfa leaf spot in Xinjiang [J]. Xinjiang Agricultural Sciences, 2020, 57(10): 1863−1870.(in Chinese)
[8]	雷娅红, 况卫刚, 郑春生, 等. 基于DNA条形码技术对镰刀菌属的检测鉴定 [J]. 植物保护学报, 2016, 43(4):544−551. LEI Y H, KUANG W G, ZHENG C S, et al. Detection and identification of the genus Fusarium by DNA barcoding [J]. Acta Phytophylacica Sinica, 2016, 43(4): 544−551.(in Chinese)
[9]	王梦奇, 白庆荣, 王大川, 等. 吉林省大豆茎点霉叶斑病病原鉴定 [J]. 大豆科学, 2019, 38(3):428−433, 454. WANG M Q, BAI Q R, WANG D C, et al. Pathogen identification of soybean leaf spot caused by Boeremia exigua var. exigua in Jilin Province [J]. Soybean Science, 2019, 38(3): 428−433, 454.(in Chinese)
[10]	王梦奇. 东北地区茎点霉属真菌研究[D]. 长春: 吉林农业大学, 2019. WANG M Q. Study of Phoma in northeast China[D]. Changchun: Jilin Agricultural University, 2019. (in Chinese).
[11]	方中达. 植病研究法[M]. 第3版. 北京: 中国农业出版社, 2001.
[12]	BOEREMA G H, GRUYTER J D, NOORDELOOS M E, et al. Phoma identification manual[M]. CABI publishing, 2004.
[13]	BIAN J Y, FANG Y L, SONG Q, et al. The fungal endophyte Epicoccum dendrobii as a potential biocontrol agent against Colletotrichum gloeosporioides [J]. Phytopathology, 2021, 111（2）: 293-303. DOI: 10.1094/PHYTO-05-20-0170-R.
[14]	VENKATASUBBAIAH P, DYKE C G V, CHILTON W S. Phytotoxic metabolites of Phoma sorghina, a new foliar pathogen of pokeweed [J]. Mycologia, 1992, 84(5): 715−723. doi: 10.1080/00275514.1992.12026197
[15]	朱香. Epicoccum sorghinum的分离及多基因转玉米株系的抗性鉴定[D]. 武汉: 华中农业大学, 2018. ZHU X. Isolation of Epicoccum sorghinum and resistance identification of multi-transgenes Maizei lines[D]. Wuhan: Huazhong Agricultural University, 2018. (in Chinese).
[16]	聂秀美, 赵桂琴, 孙浩洋, 等. 天祝县燕麦叶斑病的发生情况及其病原鉴定 [J]. 草地学报, 2019, 27(5):1384−1391. doi: 10.11733/j.issn.1007-0435.2019.05.034 NIE X M, ZHAO G Q, SUN H Y, et al. Occurrence and pathogenic identification of Oat leaf spot in Tianzhu county [J]. Acta Agrestia Sinica, 2019, 27(5): 1384−1391.(in Chinese) doi: 10.11733/j.issn.1007-0435.2019.05.034
[17]	江涛, 胡媛媛, 马良, 等. 细交链孢菌酮酸人工抗原的制备 [J]. 食品科学, 2014, 35(19):153−157. doi: 10.7506/spkx1002-6630-201419032 JIANG T, HU Y Y, MA L, et al. Preparation of artificial antigens for tenuazonic acid [J]. Food Science, 2014, 35(19): 153−157.(in Chinese) doi: 10.7506/spkx1002-6630-201419032
[18]	DU L, ZHU T, FANG Y, et al. Aspergiolide A, a novel anthraquinone derivative with naphtha [1, 2, 3-de] chromene-2, 7-dione skeleton isolated from a marine-derived fungus Aspergillus glaucus [J]. Tetrahedron, 2007, 38(24): 1085−1088.
[19]	OLIVEIRA R C, DAVENPORT K W, HOVDE B, et al. Draft genome sequence of sorghum grain mold fungus Epicoccum sorghinum, a producer of tenuazonic acid [J]. Genome Announcements, 2017, 5(4): e01495−16.
[20]	LIU X Y, HU T L, CAO K Q. Biological characteristics of strain F603 of Epicoccom sp., an antagonistic fungus for controlling Phytophthora infestans [J]. Frontiers of Agriculture in China, 2007, 1(2): 175−178. doi: 10.1007/s11703-007-0029-4