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CHEN Z Q, CHEN S B, GUO X R, et al. BSA-Seq Identification of Blast-resistance Genes in Gufeng B Rice [J]. Fujian Journal of Agricultural Sciences,2021,36(1):1−6
Citation: CHEN Z Q, CHEN S B, GUO X R, et al. BSA-Seq Identification of Blast-resistance Genes in Gufeng B Rice [J]. Fujian Journal of Agricultural Sciences,2021,36(1):1−6

BSA-Seq Identification of Blast-resistance Genes in Gufeng B Rice

  • Received Date: 2020-11-03
  • Rev Recd Date: 2020-11-18
  • Available Online: 2020-11-24
  •   Objective   To identify and map the blast-resistance gene(s) in Gufeng B, a rice cultivar that confers strong, broad-spectrum, durable resistance against various blast strains.   Method   F1 and F2 population of the cross between Gufeng B and Nipponbare were inoculated with Magnaporthe grisea 501-3 to analyze the inheritance mechanism of the blast resistance in rice. From F2, cultivars extremely resistant or sensitive to the blast were selected to establish contrasting DNA pools. Subsequently, the associated loci were mapped by means of the bulked segregation analysis (BSA-Seq).   Result  Gufeng B exhibited high resistance to all tested strains including KJ201, RB22, CHNOS, RB6, 2Y838-1, 501-3, and IR16-1. It suggested that the cultivar carried broad-spectrum, highly resistant genes toward the disease. And, since the F1 progenies showed apparent resistance against 501-3 and IR16-1, but F2 failed to display a 3 1 ratio on the resistance vs. susceptibility separation, the disease resistance was presumably controlled by multiple loci in Gufeng B. The re-sequencing of entire genomes on the DNA pools of F2 and the parental Gufeng B and Nipponbare identified 1 756 964 SNPs. The calculated △SNP-index indicated two candidate loci related to the blast resistance to be located at Chr.6: 10 082-11 397 Kb and Chr.11: 120-266 Kb. Of the two, Chromosome 6 had a region containing 4 006 SNPs and 623 InDels markers that corresponded to the Pi2/9 disease resistance locus, while Chromosome 11 had one consisting of 752 SNPs and 195 InDels.   Conclusion   The resistance of Gufeng B to 501-3 blast strain might be jointly regulated by the responsible genes in Chromosome 6 and Chromosome 11. The information would be of value in refined mapping and cloning of the resistance genes in Gufeng B as well as in selecting markers for the related studies.
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