Effects of Feeding <i>Schizochytrium</i> on Anti-<i>Vibrio vulnificus</i> Infection in Zebrafish

CHEN Hua; ZHANG Li-juan; LI Su-yi; KE Ling; CHEN Xu; LIN Chen-tao

doi:10.19303/j.issn.1008-0384.2019.10.001

Volume 34 Issue 10

Oct. 2019

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Article Navigation > Fujian Journal of Agricultural Sciences > 2019 > 34(10): 1117-1123

CHEN H, ZHANG L J, LI S Y, et al. Effects of Feeding Schizochytrium on Anti- Vibrio vulnificus Infection in Zebrafish [J]. Fujian Journal of Agricultural Sciences，2019，34（10）：1117−1123. doi: 10.19303/j.issn.1008-0384.2019.10.001

Citation:

CHEN H, ZHANG L J, LI S Y, et al. Effects of Feeding Schizochytrium on Anti- Vibrio vulnificus Infection in Zebrafish [J]. Fujian Journal of Agricultural Sciences，2019，34（10）：1117−1123. doi: 10.19303/j.issn.1008-0384.2019.10.001

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Effects of Feeding Schizochytrium on Anti-Vibrio vulnificus Infection in Zebrafish

doi: 10.19303/j.issn.1008-0384.2019.10.001

CHEN Hua¹,
ZHANG Li-juan¹,
LI Su-yi¹,
KE Ling¹,
CHEN Xu^{1
,
,},
LIN Chen-tao^{2, 3, 4
,
,}

1.
Institute of Biotechnology, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350003, China
2.
College of Life Sciences, Fujian Normal University, Fuzhou, Fujian　350007, China
3.
Center of Engineering Technology Research for Microalgae Germplasm Improvement of Fujian, Southern Institute of Oceanography, Fujian Normal University, Fuzhou, Fujian　350117, China
4.
Fujian Key Laboratory of Special Marine Bio-resources Sustainable Utilization, Fujian Normal University, Fuzhou, Fujian　350117, China

Received Date: 2019-09-05
Rev Recd Date: 2019-10-08
Publish Date: 2019-10-01

Abstract

Abstract

Objective A feeding experiment was conducted in order to study the effects of feeding Schizochytrium on the resistance of fish to bacterial infection. Methods Six-month adult zebrafishes were fed with diety added 3% Schizochytrium(Group S) for 28 days in a laboratory culture system, and the control with only basic diety(Group C). Then Vibrio vulnificus FJ03-X2 was applied for infection, and the relative survival percent of zebrafishes were analyzed. And RNA was extracted from the kidney tissues and reverse transcribed into cDNA to detect the experison of lysozyme and tnfb. Results The survival rate of Group S was significantly increased with immune protective rate reaching 42.8%. The mRNA level of Lysozyme in the kidney of zebrafish in Group S was significantly higher than that in the control (P＜0.01), while the mRNA level of TNFb was significantly lower than that in the control group (P＜0.01). Conclusion As a nutrient additive, Schizochytrium could improve the ability of zebrafish to resist bacterial infection, which provided a theoretical basis for the application of Schizochytrium in fishery industry.
- Schizochytrium,
- zebrafish,
- Vibrio vulnificus,
- feed additive

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References(25)

References

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