Bioinformatics and Construction of Expression Vector for Longan <i>MYB14</i>

LI Haoran; DING Feng; ZHANG Shuwei; PENG Hongxiang; PAN Jiechun; HE Xinhua; WANG Ying; LI Lin; WANG Jinying; HUANG Xing; TAN Chunlu; XU Jiongzhi

doi:10.19303/j.issn.1008-0384.2020.09.002

Volume 35 Issue 9

Sep. 2020

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Article Navigation > Fujian Journal of Agricultural Sciences > 2020 > 35(9): 929-936

LI H R, DING F, ZHANG S W, et al. Bioinformatics and Construction of Expression Vector for Longan MYB14 [J]. Fujian Journal of Agricultural Sciences，2020，35（9）：929−936 doi: 10.19303/j.issn.1008-0384.2020.09.002

Citation:

LI H R, DING F, ZHANG S W, et al. Bioinformatics and Construction of Expression Vector for Longan MYB14 [J]. Fujian Journal of Agricultural Sciences，2020，35（9）：929−936 doi: 10.19303/j.issn.1008-0384.2020.09.002

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Bioinformatics and Construction of Expression Vector for Longan MYB14

doi: 10.19303/j.issn.1008-0384.2020.09.002

LI Haoran^1
,,
DING Feng^{1, 2, 3
,
,},
ZHANG Shuwei⁴,
PENG Hongxiang⁴,
PAN Jiechun²,
HE Xinhua^{2, 3},
WANG Ying²,
LI Lin²,
WANG Jinying²,
HUANG Xing²,
TAN Chunlu²,
XU Jiongzhi^{2
,
,}

1.
Guangxi Academy of Agricultural Sciences/Guangxi Key Open Laboratory of Crop Genetic Improvement Biotechnology, Nanning, Guangxi　530007, China
2.
College of Agriculture, Guangxi University, Nanning, Guangxi　530004, China
3.
State Key Laboratory of Conservation and Utilization of Subtropical Agricultural Biological Resources, Nanning, Guangxi　530004, China
4.
Institute of Horticulture, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi　530007, China

Received Date: 2020-03-26
Rev Recd Date: 2020-08-01
Publish Date: 2020-09-28

Abstract

Abstract

Objective MYB14 in Longan was cloned, its bioinformatics studied, and expression vector constructed. Method Full-length sequence of MYB14 was obtained by transcriptome sequencing and bioinformatics analysis. Accordingly, specific primers for the cloning were designed, bioinformatics on the open reading frame sequence established, and overexpression vector constructed. Result In the TBP-like subfamily of MYB-related family of genes, MYB14 had an 831 bp ORF encoding 276 amino acids with one MYB binding site. It was a non-transmembrane hydrophilic protein with 57 amino acid phosphorylation sites in the cytoplasm. Its secondary structure was irregular crimp and α-helix. The predicted secondary and tertiary structures were highly consistent. The phylogenetic tree on the gene of longan showed a homology with that of cannabis. Conclusion MYB14 of Longan was successfully cloned with the constructed expression vector, PBI121-MYB14, which provided reference for the further study on the function of MYB14.
- Longan,
- MYB-related,
- gene cloning,
- expression vector construction

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References(23)

References

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