Isolation, Identification, and Evaluation of Phoxim-degrading Bacterium, D39

ZHAO Xiaoyan; ZHOU Fangyuan; WU Xiaoqing; SHI Ya-wei; ZHOU Hongzi; ZHANG Guangzhi; FAN Susu; XIE Xueying; PAN Meilin; ZHANG Xinjian

doi:10.19303/j.issn.1008-0384.2020.09.011

Volume 35 Issue 9

Sep. 2020

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Article Navigation > Fujian Journal of Agricultural Sciences > 2020 > 35(9): 997-1003

ZHAO X Y, ZHOU F Y, WU X Q, et al. Isolation, Identification, and Evaluation of Phoxim-degrading Bacterium, D39 [J]. Fujian Journal of Agricultural Sciences，2020，35（9）：997−1003 doi: 10.19303/j.issn.1008-0384.2020.09.011

Citation:

ZHAO X Y, ZHOU F Y, WU X Q, et al. Isolation, Identification, and Evaluation of Phoxim-degrading Bacterium, D39 [J]. Fujian Journal of Agricultural Sciences，2020，35（9）：997−1003 doi: 10.19303/j.issn.1008-0384.2020.09.011

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Isolation, Identification, and Evaluation of Phoxim-degrading Bacterium, D39

doi: 10.19303/j.issn.1008-0384.2020.09.011

1.
Shandong Provincial Key Laboratory of Applied Microbiology,Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan　250103, China
2.
Beijing Aerospace wks Environmental Technology Company Limited, Beijing. Fengtai　100071, China

Received Date: 2020-08-07
Rev Recd Date: 2020-09-07
Publish Date: 2020-09-28

Abstract

Abstract

Object Bacteria capable of degrading phoxim were isolated from field soils and identified by 16S rDNA sequencing prior to characterization and evaluation for pollution abatement of the organophosphate insecticide on farmland. Possibility of utilizing the bacterium for disease prevention was also explored. Method Phoxim-degrading bacteria were isolated on the enrichment culture and identified by 16S rDNA sequences analysis combined with morphological, physiological, and biochemical characteristics. Specific agent in the bacterium responsible for the degradation was located by a plate activity test, and its degrading effect on phoxim-containing wheat bran determined by HPLC. For possible application in disease preventions, the efficacy of the isolated bacteria on fungal phytopathogens was tested with a dual-culture agar plating technique. Result A phoxim-degrading bacterium designated as D39 was isolated from the soil polluted by the pesticide and primarily identified as Delftia sp. The endoenzyme in D39 was determined to be the key agent responsible of the insecticide degradation. The enzyme was, thus, extracted to spray at a concentration of 0.10 g·kg⁻¹ onto wheat bran spiked with 300 mg·kg⁻¹ of phoxim. As measured by HPLC, the treatment produced a 100% phoxim reduction on the bran after 11 h at 25 ℃. Separately, the antagonism study by the dual-culture plating showed that D39 affected 5 tested phytopathogens in varying degrees. The highest average inhibition rate exerted by D39 was 50.00% on Rhizotonia cerealis, and the lowest 21.05% on Curvularia lunata. Conclusion D39 could not only efficiently degrade phoxim residues on wheat bran but also inhibit the growth of a certain phytopathogens. It appeared that the extracted endoenzyme from D39 could be applied on grains to decompose the residual insecticide for food safety improvement and served as a disease prevention bioagent as well.
- Phoxim-degrading bacterium,
- isolation,
- identification,
- inhibition rate,
- endoenzyme

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References(15)

References

[1]	周云全, 刘春宇, 曲冠男, 等. 农药辛硫磷的密度泛函理论计算及拉曼光谱分析 [J]. 原子与分子物理学报, 2020, 37(3):331−336. ZHOU Y Q, LIU C Y, QU G N, et al. Density functional theory calculation and Raman spectrum analysis of the pesticidephoxim [J]. Journal of Atomic and Molecular Physics, 2020, 37(3): 331−336.(in Chinese
[2]	刘飞翔, 王小梅, 吴文睿, 等. 高效液相色谱法测定小麦和大米中辛硫磷残留的快速前处理方法 [J]. 食品安全质量检测学报, 2019, 10(10):2989−2994. doi: 10.3969/j.issn.2095-0381.2019.10.025 LIU F X, WANG X M, WU W R, et al. Rapid pretreatment for the determination of phoxim residues in wheat and rice by high performance liquid chromatography [J]. Journal of Food Safety & Quality, 2019, 10(10): 2989−2994.(in Chinese doi: 10.3969/j.issn.2095-0381.2019.10.025
[3]	崔新刚, 李百祥. 辛硫磷与高效氯氰菊酯对雄性大鼠精子的联合作用 [J]. 牡丹江医学院学报, 2016, 37(6):1−4. CUI X G, LI B X. Joint toxic effect of phoximand and beta-cypermethrin on the sperm system of male rats [J]. Journal of Mudanjiang Medical University, 2016, 37(6): 1−4.(in Chinese
[4]	李宏辉, 刘秀芳, 马潇, 等. 辛硫磷和灭多威混配对雄性小鼠生殖细胞的毒性作用 [J]. 宁夏医科大学学报, 2009, 31(5):582−584. doi: 10.3969/j.issn.1674-6309.2009.05.008 LI H H, LIU X F, MA X, et al. Toxic effects of phoxim and methomyl on male reproductive cells [J]. Journal of Ningxia Medical University, 2009, 31(5): 582−584.(in Chinese doi: 10.3969/j.issn.1674-6309.2009.05.008
[5]	李松花, 金文红, 李铁骥. 辛硫磷对小鼠心脏CHE和SOD活性的影响 [J]. 中国卫生工程学, 2011, 10(1):12−13, 17. LI S H, JIN W H, LI T J. Activity of cordis cholinesterase and superoxide dismutase in mice exposed to phoxim [J]. Chinese Journal of Public Health Engineering, 2011, 10(1): 12−13, 17.(in Chinese
[6]	王运儒, 秦玉燕, 农耀京, 等. 高效液相色谱法测定马铃薯和土壤中的辛硫磷残留 [J]. 食品安全质量检测学报, 2017, 8(2):421−427. WANG Y R, QIN Y Y, NONG Y J, et al. Determination of phoxim residues in potato and soil by high performance liquid chromatography [J]. Journal of Food Safety & Quality, 2017, 8(2): 421−427.(in Chinese
[7]	张其才, 饶钦雄, 汤倩倩, 等.三种有机磷农药在双孢蘑菇及其栽培基质中的残留动态[J]. 农药学学报, 2020, 22: 1-11. ZHANG Q C, RAO Q X, TANG Q Q, et al. The residual dynamics of three organophosphorus pesticides in Agaricus bisporus fruits and cultivation substrates[J]. Chinese Journal of Pesticide Science, 2017, (8)2: 421-427.
[8]	沈雨佳, 洪源范, 洪青, 等. 辛硫磷降解菌XSP-1的分离、鉴定及其降解特性研究 [J]. 环境科学, 2007, 28(12):2833−2837. doi: 10.3321/j.issn:0250-3301.2007.12.029 SHEN Y J, HONG Y F, HONG Q, et al. Isolation, identification and characteristics of a phoxim-degrading bacterium XSP-1 [J]. Chinese Journal of Environmental Science, 2007, 28(12): 2833−2837.(in Chinese doi: 10.3321/j.issn:0250-3301.2007.12.029
[9]	李芳, 黄素芳, 刘波. 淡紫拟青霉对辛硫磷的降解效应 [J]. 应用与环境生物学报, 2006, 12(1):104−107. doi: 10.3321/j.issn:1006-687X.2006.01.025 LI F, HUANG S F, LIU B. Degradation of phoxim by paecilomyces lilaciuns [J]. Chinese Journal of Applied & Environmental Biology, 2006, 12(1): 104−107.(in Chinese doi: 10.3321/j.issn:1006-687X.2006.01.025
[10]	张瑞福, 朱卫, 崔中利, 等. 辛硫磷降解菌X-1的分离鉴定及降解性状的初步研究 [J]. 环境科学学报, 2003, 23(3):411−413. doi: 10.3321/j.issn:0253-2468.2003.03.025 ZHANG R F, ZHU W, CUI Z L, et al. Isolation, identification and characters of phoxim degrading bacterium [J]. Acta Scientiae Circumstantiae, 2003, 23(3): 411−413.(in Chinese doi: 10.3321/j.issn:0253-2468.2003.03.025
[11]	于婷, 侯长明, 王海荣, 等. 嗜铁细菌枯草芽胞杆菌Bs-15对辛硫磷的降解特性 [J]. 应用与环境生物学报, 2014, 20(2):179−184. YU T, HOU C M, WANG H R, et al. Characteristics in degradation of phoxim by siderophore producing bacterium Bacillus subtilis Bs-15 [J]. Chinese Journal of Applied & Environmental Biology, 2014, 20(2): 179−184.(in Chinese
[12]	东秀珠, 蔡妙英. 常见细菌系统鉴定手册[M]. 北京:科学出版社, 2001.
[13]	周永丹, 张勇, 李存雄, 等. HPLC法同步测定谷物中的吡虫啉和辛硫磷 [J]. 江西师范大学学报(自然科学版), 2015, 39(6):566−569. ZHOU Y D, ZHANG Y, LI C X, et al. The determination of imidacloprid and phoxim residues in cereals by HPLC [J]. Journal of Jiangxi Normal University (Natural Sciences Edition), 2015, 39(6): 566−569.(in Chinese
[14]	侯晓慧, 薛科宇, 魏珂, 等. 超高效液相色谱-飞行时间质谱法测定蔬菜中辛硫磷残留量 [J]. 食品安全质量检测学报, 2018, 9(17):4645−4649. doi: 10.3969/j.issn.2095-0381.2018.17.029 HOU X H, XUE K Y, WEI K, et al. Determination of phoxim residue in vegetables by ultra performance liquid chromatography-quadrupole time of flight mass spectrometry [J]. Journal of Food Safety & Quality, 2018, 9(17): 4645−4649.(in Chinese doi: 10.3969/j.issn.2095-0381.2018.17.029
[15]	赵晓燕, 洪永聪, 郭凯, 等. TL2的鉴定及其对植物病原菌的抑制作用 [J]. 福建农业学报, 2013, 28(4):353−356. doi: 10.3969/j.issn.1008-0384.2013.04.010 ZHAO X Y, HONG Y C, GUO K, et al. Identification of TL2 and the inhibitive effect of TL2 to fungal pathogens [J]. Fujian Journal of Agricultural Sciences, 2013, 28(4): 353−356.(in Chinese doi: 10.3969/j.issn.1008-0384.2013.04.010