青花菜<i>BoBURP</i>2基因的克隆与表达分析

章燕如; 许鑫; 祝琦; 周秀倩; 俞可可; 龚秀; 蒋明

doi:10.19303/j.issn.1008-0384.2016.09.007

青花菜BoBURP2基因的克隆与表达分析

doi: 10.19303/j.issn.1008-0384.2016.09.007

台州学院生命科学学院, 浙江椒江 318000

基金项目:

国家级大学生创新创业训练计划项目 201510350011

浙江省公益性技术应用研究计划项目 2016C32091

台州市科技计划项目 162ny14

详细信息

作者简介:
章燕如(1994-),女,研究方向:植物分子生物学(E-mail:674155045@qq.com)

通讯作者:
蒋明(1973-),男,副教授,研究方向:植物逆境生物学及其分子调控(E-mail:jiangming1973@139.com)

中图分类号: Q78
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- 被引次数: 0
出版历程
- 收稿日期: 2016-07-10
- 修回日期: 2016-08-26
- 刊出日期: 2016-09-28

Cloning and Expression Analysis on BoBURP2 Gene of Brassica oleracea var. italica

College of Life Sciences, Taizhou University, Jiaojiang, Zhejiang 318000, China

摘要

摘要: BURP是植物特有蛋白，在生长发育和抗逆反应中起着重要作用。本研究以青花菜为材料，利用PCR克隆到1个BURP基因，命名为BoBURP2。测序结果表明，BoBURP2的基因组DNA全长为1 218 bp，具1个内含子，编码区全长为840 bp，编码279个氨基酸；序列比对结果表明，该基因与BURP家族成员RD22具有较高的同源性。系统发育分析结果表明，BoBURP2与甘蓝、芜菁和甘蓝型油菜的同源序列相似性最高，在发育树上聚为一组，与醉蝶花的相似性最低，亲缘关系最远。RT-PCR结果表明，BoBURP2的表达受NaCl和干旱胁迫的诱导，表达量分别在24 h和48 h时达到最大值，暗示该基因与这2种逆境响应相关。青花菜BoBURP2的克隆与表达分析，为后续基因功能的鉴定和抗逆分子育种奠定了基础。
- 青花菜 /
- BoBURP2 /
- RD22 /
- 克隆 /
- 表达
Abstract: A plant-specific protein, BURP plays an important role in the growth, development, and stress response of a plant. In this study, a BURP gene designated as BoBURP2 was isolated from broccoli, Brassica oleracea var. italica, using PCR. The gene sequencing showed its genome DNA was 1 218 bp with a single intron. The complete coding sequence was 840 bp in length, encoding 279 amino acids. It shared a high homology with RD22, which is also a member of the BURP family. Phylogenetic analysis on BoBURP2 showed its sequence to be highly similar to those of B. oleracea, B. rapa, and B. napus. They could possibly be grouped in a same clade. On the other hand, the low similarity it shared with the sequence of Tarenaya hassleriana suggested a remote relationship between them. The RT-PCR results indicated the expression of BoBRUP2 was induced by both NaCl and drought stresses, with the highest levels detected at 24 h and 48 h. The cloning and expression analysis on BoBURP2 provided the information for further studies on the identification of its genetic function as well as the molecular breeding for stress resistant B. oleracea var. italica.
- Brassica oleracea var. italica /
- BoBURP2 /
- RD22 /
- cloning /
- expression

HTML全文

图 1 BoBURP2基因的编码区及推导的氨基酸序列

注：阴影部分为BURP结构域。

Figure 1. Coding sequence of BoBURP2 gene and its deduced amino acid sequence

下载: 全尺寸图片幻灯片

图 2 BoBURP2与8种同源氨基酸的序列比对

Figure 2. Sequence alignments of BoBURP2 and 8 homologous amino acids

下载: 全尺寸图片幻灯片

图 3 用NJ法构建的BURP进化树

Figure 3. Phylogenetic tree of BURP constructed using NJ methods

下载: 全尺寸图片幻灯片

图 4 BoBURP2基因在NaCl和干旱胁迫下的表达

注：A为NaCl；B、D和F为肌动蛋白内标；C为干旱；E为对照。

Figure 4. Expression of BoBURP2 under NaCl and drought stresses

下载: 全尺寸图片幻灯片

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