Effect of H2S on Morphology and Physiological/Biochemical Properties of Wheat
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摘要: 采用硫氢化钠作为硫化氢的外源性供体，研究不同浓度硫氢化钠（0、0.05、0.10、0.20、0.40、0.80、1.60、3.20 mmol·L-1）对小麦幼苗形态、生理生化的影响，以探寻H2S对小麦幼苗生长发育的机制。结果表明，低浓度硫氢化钠处理促进了小麦幼苗的生长，表现为株高、根长、鲜重和干重的增加；而高浓度的硫氢化钠处理则明显抑制了小麦幼苗的生长，甚至伤害，表现为株高、根长、鲜重和干重的减少。同样，不同浓度的硫氢化钠处理也显著引起了植株的含水量、叶片的叶绿素含量的变化。运用主成分法分析发现，小麦幼苗生物量、生理指标等数据之间存在着显著相关性。Abstract: NaHS was used as an exogenous H2S for this study to induce changes on the protein expression and morphological/physiological properties of wheat seedlings. Varied concentrations of NaHS (i.e., 0, 0.05, 0.10, 0.20, 0.40, 0.80, 1.60, and 3.20 mmol·L-1) were incorporated in the medium solution for the experimentation. According to the effect on the seedling growth and based on their concentrations, 3 groups of the solutions were classified as (a) LC at 0.05 and 0.10 mmol·L-1 that promoted the growth, (b) IC at 0.20, 1.60, and 0.40 mmol·L-1 that inhibited the growth, and (c) HC at 0.80 and 3.20 mmol·L-1 that harmed the growth. When LC was applied, the height, root length, and fresh or dry weight of the plants increased significantly, while the application of HC produced the opposite effects. Furthermore, moisture and leaf chlorophyll contents of the plants were significantly affected by the NaHS concentration. There were also significant correlations observed between the biomass and physiological characteristics, according to a principal component analysis.
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Key words:
- hydrogen sulfide /
- wheat /
- morphology /
- physiology and biochemistry /
- correlation
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图 1 不同浓度NaHS对小麦幼苗株高的影响
Figure 1. Effect of NaHS concentration on plant height of wheat seedlings
图 2 不同浓度NaHS对小麦幼苗根长的影响
Figure 2. Effect of NaHS concentration on root length of wheat seedlings
图 3 不同浓度NaHS对小麦幼苗鲜重的影响
Figure 3. Effect of NaHS concentration on fresh weight of wheat seedlings
图 4 不同浓度NaHS对小麦幼苗生物量的影响
Figure 4. Effect of NaHS concentration on biomass of wheat seedlings
图 5 不同浓度NaHS对小麦幼苗含水量的影响
Figure 5. Effect of NaHS concentration on moisture content of wheat seedlings
图 6 不同浓度NaHS对小麦幼苗叶绿素含量的影响
Figure 6. Effect of NaHS concentration on chlorophyll content in leaves of wheat seedlings
图 7 探索性因子分析实现NaHS处理组聚类
Figure 7. Exploratory factor analysis for clustering NaHS treatment groups
图 8 促进组小麦幼苗形态和生理生化指标主成分分析
Figure 8. Principal components analysis on morphological and physiological/biochemical properties of wheat seedlings in growth-promoting group
图 9 抑制组小麦幼苗形态和生理生化指标主成分分析
Figure 9. Principal component analysis on morphological and physiological/biochemical properties of wheat seedlings in growth-inhibiting group
图 10 伤害组小麦幼苗形态和生理生化指标的主成分分析
Figure 10. Principal component analysis on morphological and physiological/biochemical properties of wheat seedlings in growth-harming group
表 1 不同浓度NaHS处理组之间距离相似性分析
Table 1. Similarity on disparities between treatments with varied NaHS concentrations
浓度组/(mmol·L-1) 0 0.05 0.10 0.20 0.40 0.80 1.60 3.20 0 1.000 0.05 0.997 1.000 0.1 0.996 1.000 1.000 0.2 0.986 0.970 0.970 1.000 0.4 0.969 0.951 0.951 0.994 1.000 0.8 0.942 0.919 0.919 0.983 0.996 1.000 1.6 0.979 0.966 0.966 0.994 0.998 0.989 1.000 3.2 0.920 0.895 0.896 0.966 0.987 0.996 0.977 1.000 
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表 2 不同浓度NaHS处理小麦幼苗不同浓度组的探索性因子分析
Table 2. Exploratory factor analysis on treatment groups of wheat seedlings treated by varied NaHS concentrations
NaHS处理浓度/(mmol·L-1) 第一主因子 第二主因子 0 0.601 0.799 0.05 0.553 0.833 0.1 0.555 0.831 0.2 0.711 0.700 0.4 0.767 0.641 0.8 0.818 0.574 1.6 0.733 0.679 3.2 0.850 0.525 特征值 7.800 0.189 贡献率/% 97.503 2.361 累积贡献率/% 97.503 99.864
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