利用物质组学方法筛选产游离油酸的芽胞杆菌菌株

肖通奋; 王阶平; 刘波; 陈峥; 刘国红; 陈倩倩; 车建美; 葛慈斌

doi:10.19303/j.issn.1008-0384.2016.12.016

利用物质组学方法筛选产游离油酸的芽胞杆菌菌株

doi: 10.19303/j.issn.1008-0384.2016.12.016

1.
福州大学生物科学与工程学院, 福建福州 350108
2.
福建省农业科学院农业生物资源研究所, 福建福州 350003

基金项目:

国家自然科学基金 31370059

福建省重大专项项目农业科技重大专项 2015NZ0003-1

福建省种业创新与产业化工程项目 FJZZZY-1544

福建省农业科学院人才引进科研启动基金项目 YJRC2014-1

福建省科技计划项目--省属公益类科研院所基本科研专项 2014R1101016-11

详细信息

作者简介:
肖通奋(1989-), 男, 硕士研究生, 研究方向:微生物学

通讯作者:
刘波(1957-), 男, 研究员, 研究方向:微生物生物技术与农业生物药物(E-mail:fzliubo@163.com)

中图分类号: Q939
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- 被引次数: 0
出版历程
- 收稿日期: 2016-05-10
- 修回日期: 2016-08-05
- 刊出日期: 2016-12-28

Oleic-acid-producing Bacillus-like Bacteria Screened by Means of Chemomics

1.
College of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian 350108, China
2.
Institute of Agro-biological Resources, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China

摘要

摘要: 利用高效液相色谱-四级杆飞行时间质谱联用技术测定了52个芽胞杆菌Bacillus-like菌株发酵上清液中的代谢物组成，各物质的分子特征通过MassHunter软件从原始数据中进行提取，然后通过Metlin代谢物质谱数据库的检索比对，获得各代谢物的基本信息。结果表明，在海绵芽胞杆菌Bacillus plakortidis菌株FJAT-14858的发酵上清液中，可以检测出游离油酸，其Metlin匹配得分达95.61，其相对含量占发酵上清液总代谢物的1.62%，精确质量数为284.2693。海绵芽胞杆菌菌株FJAT-14858是首个被发现产非毒性的游离油酸的芽胞杆菌菌株，本研究为新型微生物油脂的开发与利用提供菌种资源和理论依据。
- 海绵芽胞杆菌FJAT-14858 /
- 游离油酸 /
- 物质组学 /
- 高效液相色谱-四级杆飞行时间质谱
Abstract: Extracellular metabolites in the supernatants 52 Bacillus-like strains were analyzed by HPLC along with the quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF-MS). Subsequently, the molecular feature extraction (MFE) was performed on the data using the software MassHunter followed by submitting the information for comparison against the mass spectrometry database, Metlin. Free oleic acid was thereby detected in the cultural supernatant of Bacillus plakortidis, FJAT-14858. Its matching score with oleic acid was 95.61, accurate mass was 284.2693, and relative content in the metabolites collected was up to 1.62%. To date, B. plakortidis FJAT-14858 was the first Bacillus-like bacterium known to produce free oleic acid with no cytotoxicity to the bacteria per se.
- Bacillus plakortidis FJAT-14858 /
- free oleic acid /
- chemomics /
- HPLC-quadrupole time-of-flight MS

HTML全文

图 1 海绵芽胞杆菌FJAT-14858的油酸离子提取色谱

Figure 1. Extracted ion chromatogram (EIC) of oleic acid from FJAT-14858

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图 2 海绵芽胞杆菌FJAT-14858中油酸扫描质谱

Figure 2. Scanning mass spectrum of oleic acid from FJAT-14858

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图 3 海绵芽胞杆菌FJAT-14858中油酸分子特征提取质谱

Figure 3. MFE spectrum of oleic acid from FJAT-14858

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表 1 供试芽胞杆菌菌株及产游离油酸的芽胞杆菌筛选结果

Table 1. Bacillus-like bacteria screened and FJAT-14858 that produced free oleic acid in liquid culture medium

序号	菌株编号	学名	中文名称	保留时间	匹配度	相对含量	精确分子量	质核比
1	FJAT-14205	Aneurinibacillus migulanus	米氏解硫胺素芽胞杆菌			0
2	FJAT-14856	Aneurinibacillus aneurinilyticus	解硫胺素硫胺素芽胞杆菌			0
3	FJAT-10042	Fictibacillus barbaricus	奇异虚构芽胞杆菌			0
4	FJAT-10043	Fictibacillus bataviensis	奇异虚构芽胞杆菌			0
5	FJAT-16152	Bacillus cereus	蜡样芽胞杆菌			0
6	FJAT-14221	Bacillus acidiceler	酸快生芽胞杆菌			0
7	FJAT-10032	Bacillus fordii	福氏芽胞杆菌			0
8	FJAT-10014	Bacillus alcalophilus	嗜碱芽胞杆菌			0
9	FJAT-14272	Bacillus cibi	食物芽胞杆菌			0
10	FJAT-14236	Bacillus butanolivorans	食丁酸芽胞杆菌			0
11	FJAT-14270	Bacillus ginsengihumi	人参土芽胞杆菌			0
12	FJAT-10033	Bacillus fortis	强壮芽胞杆菌			0
13	FJAT-520	Bacillus coagulaus	凝结芽胞杆菌			0
14	FJAT-10010	Bacillus endophyticus	内生芽胞杆菌			0
15	FJAT-14212	Bacillus indicus	印度芽胞杆菌			0
16	FJAT-14252	Bacillus isronensis	印空研芽胞杆菌			0
17	FJAT-8757	Bacillus badius	栗褐芽胞杆菌			0
18	FJAT-16342	Bacillus cereus	蜡样芽胞杆菌			0
19	FJAT-16366	Bacillus cereus	蜡样芽胞杆菌			0
20	FJAT-8762	Bacillus clausii	克劳芽胞杆菌			0
21	FJAT-14215	Bacillus cecembensis	科研中心芽胞杆菌			0
22	FJAT-16072	Bacillus megaterium	巨大芽胞杆菌			0
23	FJAT-10015	Alicyclobacillus cellulosilyticus	解纤维素脂环酸芽胞杆菌			0
24	FJAT-14213	Bacillus lehensis	列城芽胞杆菌			0
25	FJAT-17931	Bacillus amyloliquefaciens	解淀粉芽胞杆菌			0
26	FJAT-17934	Bacillus amyloliquefaciens	解淀粉芽胞杆菌			0
27	FJAT-2349	Bacillus amyloliquefaciens	解淀粉芽胞杆菌			0
28	FJAT-8754	Bacillus amyloliquefaciens	解淀粉芽胞杆菌			0
29	FJAT-10037	Bacillus hemicellulosilyticus	解半纤维素芽胞杆菌			0
30	FJAT-8764	Bacillus firmus	坚强芽胞杆菌			0
31	FJAT-16022	Bacillus methylotrophicus	甲基营养芽胞杆菌			0
32	FJAT-16041	Bacillus methylotrophicus	甲基营养芽胞杆菌			0
33	FJAT-8761	Bacillus circulans	环状芽胞杆菌			0
34	FJAT-14210	Bacillus koreensis	韩国芽胞杆菌			0
35	FJAT-16291	Bacillus licheniformis	地衣芽胞杆菌			0
36	FJAT-16398	Bacillus licheniformis	地衣芽胞杆菌			0
37	FJAT-16414	Bacillus licheniformis	地衣芽胞杆菌			0
38	FJAT-16766	Bacillus licheniformis	地衣芽胞杆菌			0
39	FJAT-16777	Bacillus licheniformis	地衣芽胞杆菌			0
40	FJAT-16973	Bacillus licheniformis	地衣芽胞杆菌			0
41	FJAT-17199	Bacillus licheniformis	地衣芽胞杆菌			0
42	FJAT-17228	Bacillus licheniformis	地衣芽胞杆菌			0
43	FJAT-17433	Bacillus licheniformis	地衣芽胞杆菌			0
44	FJAT-17730	Bacillus licheniformis	地衣芽胞杆菌			0
45	FJAT-8756	Bacillus azotoformans	产氮芽胞杆菌			0
46	FJAT-8765	Bacillus.flexus	弯曲芽胞杆菌			0
47	FJAT-14220	Bacillus aryabhattai	阿氏芽胞杆菌			0
48	FJAT-16042	Bacillus aryabhattai	阿氏芽胞杆菌			0
49	FJAT-16043	Bacillus aryabhattai	阿氏芽胞杆菌			0
50	FJAT-16059	Bacillus aryabhattai	阿氏芽胞杆菌			0
51	FJAT-14227	Bacillus novalis	休闲地芽胞杆菌			0
52	FJAT-14858	Bacillus plakortidis	海绵芽胞杆菌	45.6283	95.61	1.62	284.2693	283.2622

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表 2 化合物鉴定的最佳匹配结果

Table 2. Best matching result for compound identification

Best	Name	Formula	Score	Mass	Mass (DB)	Diff (ppm)	Diff (abs.ppm)	Diff (mDa)	Species	m/z	m/z (Calc)	Diff (ppm)	Diff (mDa)	Height	Height (Calc)	Height /%	Height /% (Calc)	Hight Sum /%	Hight Sum /% (Calc)
Yes	Oleic acid (D2)	C₁₈H₃₂D₂O₂	95.61	284.2693	284.2684	-3.21	3.21	-0.91	(M-H)^-	283.2622	283.2612	-3.77	-1.1	79558.5	77929.3	100	100	83.6	81.8

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