非致病性尖孢镰刀菌固体发酵条件筛选

肖荣凤; 陈燕萍; 刘波; 葛慈斌; 朱育菁

doi:10.19303/j.issn.1008-0384.2017.012.014

非致病性尖孢镰刀菌固体发酵条件筛选

doi: 10.19303/j.issn.1008-0384.2017.012.014

福建省农业科学院农业生物资源研究所, 福建福州 350003

基金项目:

福建省自然科学基金项目 2016J01128

福建省农业科技重大专项 2015NZ0003-1

福建省农业科学院科技创新团队PI建设项目 2016PI-43

详细信息

作者简介:
肖荣凤(1977-), 女, 硕士, 副研究员, 研究方向:微生物生物技术(E-mail:xrfxiao@163.com)

通讯作者:
刘波(1957-), 男, 博士, 研究员, 研究方向:微生物生物技术与农业生物药物(E-mail:fzliubo@163.com)

中图分类号: S476
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- 被引次数: 0
出版历程
- 收稿日期: 2017-01-16
- 修回日期: 2017-09-05
- 刊出日期: 2017-12-28

Optimizing Solid-medium Fermentation of Non-pathogenic Fusarium oxysporum

Agricultural Bio-resources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China

摘要

摘要: 为优化枯萎病植物疫苗工程菌株—非致病性尖孢镰刀菌FJAT-9290固体发酵条件，以产孢量为指标，分析了培养基初始含水量、初始接种量、培养温度和培养时间对菌株产孢量的影响，筛选出最优的发酵条件。结果表明，利用组培瓶培养7 d，分别筛选出菌株生长的最佳初始含水量为50%、初始接种量为7%和培养温度为28℃，并在此基础上，利用聚丙烯塑料袋培养，发酵至20 d时菌株的产孢量最高，孢子数量达6.35×10⁸个·g^-1，较初始接菌量增加了4个数量级。
- 非致病尖孢镰刀菌 /
- 固体发酵 /
- 培养条件
Abstract: Optimal culture conditions on solid-medium for the non-pathogenic Fusarium oxysporum FJAT-9290 to produce vaccine against fusarium wilt were investigated. The initial moisture content and inoculum amount in the medium, as well as the temperature and duration for the fermentation were varied to determine their effects on the resulting yield of conidia. It appeared that, a medium with 50% moisture in a tissue culture bottle inoculated with 7% of FJAT-9290 to ferment at 28℃ for 7 ds followed by cultivation in a polypropylene bag could produce a maximized conidium count of 6.35×10⁸·g^-1 on the 20^th day. The condia count quadrupled that of the initial inoculum.
- non-pathogenic Fusarium oxysporum /
- solid-medium fermentation /
- fermentation condition

HTML全文

图 1 培养基初始含水量对菌株FJAT-9290产孢量的影响

Figure 1. Effect of initial moisture content of medium on conidium yield of FJAT-9290

下载: 全尺寸图片幻灯片

图 2 培养基初始接种量对菌株FJAT-9290产孢量的影响

Figure 2. Effect of inoculum amount on conidium yield of FJAT-9290

下载: 全尺寸图片幻灯片

图 3 培养温度对菌株FJAT-9290产孢量的影响

Figure 3. Effect of fermentation temperature on conidium yield of FJAT-9290

下载: 全尺寸图片幻灯片

图 4 培养时间对菌株FJAT-9290产孢量的影响

Figure 4. Effect of fermentation time on conidium yield of FJAT-9290

下载: 全尺寸图片幻灯片

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