Optimization of Polysaccharide Extraction from Mycelia of Selenium-rich Agaricus blazei
-
摘要: 为探讨姬松茸富硒菌丝体多糖的提取工艺，研发食用菌富硒菌丝多糖保健品，通过单因子试验和正交试验，对热水浸提法提取姬松茸富硒菌丝体多糖的条件进行优化。结果表明：料水比、乙醇沉淀浓度和浸提的温度、时间及次数对姬松茸富硒菌丝体多糖的提取均有较大影响，其最优提取条件为：料水比1：35（g·mL-1）、乙醇沉淀浓度90%、浸提温度85℃、浸提时间3.5 h、浸提次数3次，在此工艺条件下富硒菌丝体多糖的提取量及其硒含量分别达到69.5 mg·g-1和12.1 μg·g-1（菌丝干重）；本工艺简单、稳定、可行，适用于姬松茸富硒菌丝体多糖的提取。Abstract: The hot-water extraction of polysaccharides from the mycelia of Agaricus blazeia grown on a selenium-enriched medium was optimized to facilitate product development of health foods with the functional ingredient. A single factor followed by an orthogonal experiment was carried out to show the extraction yield to be significantly affected by the altered processing conditions. The optimized extraction conditions included a sample/water ratio of 1/35 (g·mL-1), an ethanol concentration for precipitation at 90%, a temperature of 85℃, and 3 repeated cycles that lasted 3.5 h. As a result, a yield on polysaccharides reached 69.5 mg·g-1; and the content of selenium in polysaccharides reached 12.1 μg·g-1(dry weight of mycelium). The procedures were simple, stable, practical, and appropriate for the extraction.
-
Key words:
- Agaricus blazei Murill /
- selenium-rich mycelium /
- polysaccharides /
- extraction
-
图 1 料水比对多糖提取量的影响
Figure 1. Effect of sample/water ratios on extraction yield of polysaccharides
图 2 浸提温度对多糖提取量的影响
Figure 2. Effect of temperature on extraction yield of polysaccharides
图 4 浸提次数对多糖提取量的影响
Figure 4. Effect of repeated cycles on extraction yield of polysaccharides
图 5 乙醇沉淀质量分数对多糖提取量的影响
Figure 5. Effect of ethanol concentration for precipitation on extraction yield of polysaccharides
表 1 L9(34)正交试验因素及水平
Table 1. Factors and levels of L9(34) orthogonal test
表 2 L9(34)正交试验结果与分析
Table 2. Results of L9(34) orthogonal test and analysis
-
[1] 吴法先.硒和富硒食用菌对人体健康的保健作用[J].山西医药研究, 1989, 3(1-2):41. http://cdmd.cnki.com.cn/Article/CDMD-10625-2003071321.htm [2] RAYMAN M P. The importance of selenium to human health[J]. The Lancet, 2000, 356(9225):233-241. doi: 10.1016/S0140-6736(00)02490-9 [3] MIZUNO M, MINATO K I, ITO H, et al. Anti-tumor polysaccharide from the mycelium of liquid-cultured Agaricus blazei Murill[J]. Biochem Mol Biol Inte, 1999, 47(4):707-714. [4] SORIMACHI K, AKIMOTO K, IKEHARA Y, et al. Secretion of TNF-alpha, IL-8 and nitric oxide by macrophages activated with Agaricus blazei Murill fractions in vitro[J]. Cell Structure and Function, 2001, 26(2):103-108. doi: 10.1247/csf.26.103 [5] 姜荷, 谷文英.姬松茸菌丝体多糖体外抗肿瘤活性的研究及结构初探[J].中草药, 2005, 36(11):1616-1618. doi: 10.3321/j.issn:0253-2670.2005.11.006 [6] 林戎斌, 林陈强, 张慧, 等.姬松茸食药用价值研究进展[J].食用菌学报, 2012, 19(2):117-122. http://www.cnki.com.cn/Article/CJFDTOTAL-ZGYA201143040.htm [7] 张惠珍, 杨淑云.姬松茸富硒培养研究[J].西南农业大学学报(S1), 2004, 6(21):95-97, 104. http://cdmd.cnki.com.cn/Article/CDMD-10625-2003071321.htm [8] 陈彻, 郝军, 楚惠媛, 等.富硒姬松茸提取物-硒蛋白多糖处理小鼠血清对K562细胞凋亡的影响及机制[J].中国新药杂志, 2007, 16(17):1361-1364. doi: 10.3321/j.issn:1003-3734.2007.17.012 [9] 陈彻, 楚惠媛, 郝军, 等.富硒姬松茸提取物硒蛋白多糖对荷瘤小鼠的影响[J].中药材, 2006, 29(11):1215-1217. doi: 10.3321/j.issn:1001-4454.2006.11.037 [10] 刘祖同, 罗信昌.食用蕈菌生物技术及应用[M].北京:清华大学出版社, 2002. [11] 刘新海, 冯培勇, 史亚丽, 等.姬松茸菌丝体多糖的分离纯化及抗疲劳研究[J].西南农业大学学报, 2006, 28(2):190-194. http://www.cnki.com.cn/Article/CJFDTOTAL-XNND200602004.htm [12] 贾薇, 刘艳芳, 张劲松, 等.姬松茸菌丝体多糖提取方法初探[J].食用菌学报, 2003, 10(3):41-44. http://www.cnki.com.cn/Article/CJFDTOTAL-HBNY201414044.htm [13] 杨立红, 梁建光, 刘新海, 等.姬松茸菌丝体多糖提取工艺最优化研究[J].山东农业大学学报:自然科学版, 2004, 35(4):489-492. http://www.cnki.com.cn/Article/CJFDTOTAL-HBNY201414044.htm [14] 杨淑云, 刘朝贵, 朱凯, 等.富硒姬松茸液体培养条件的研究[J].微生物学通报, 2003, 30(1):9-13. http://www.cnki.com.cn/Article/CJFDTOTAL-WSWT200301003.htm [15] 苗敬芝, 吕兆启.灵芝深层液体发酵多糖的提取与测定[J].食品科技, 2003, (2):13-14. http://www.cnki.com.cn/Article/CJFDTOTAL-SSPJ200302004.htm [16] 刘兴艳, 朱静平, 周浩, 等.原子荧光测定苹果中的微量硒[J].西南农业大学学报:自然科学版, 2006, 28(1):106-110. http://www.cnki.com.cn/Article/CJFDTOTAL-GXJB2012S3003.htm [17] 黄翔宇, 程度, 黄科, 等.姬松茸菌丝体与子实体多糖的提取及含量的比较[J].广东药学, 2002, 12(3):2-4. http://www.cnki.com.cn/Article/CJFDTOTAL-YAXU200203001.htm [18] 姜荷, 谷文英.姬松茸菌丝体中各组分多糖的抗肿瘤免疫活性[J].天然产物研究与开发, 2005, 17(2):152-156. http://cdmd.cnki.com.cn/Article/CDMD-10295-2005104904.htm -
下载:
点击查看大图
计量
- 文章访问数: 1230
- HTML全文浏览量: 132
- PDF下载量: 212
- 被引次数: 0
