核桃楸叶多酚提取工艺的响应面法优化及其抗氧化性分析

彭小进; 王天淼; 孙墨珑

doi:10.19303/j.issn.1008-0384.2017.07.011

核桃楸叶多酚提取工艺的响应面法优化及其抗氧化性分析

doi: 10.19303/j.issn.1008-0384.2017.07.011

东北林业大学理学院, 黑龙江哈尔滨 150040

基金项目:

中央高校基本科研业务费专项 2572015BB18

详细信息

作者简介:
彭小进(1990-), 男, 硕士研究生, 研究方向为天然产物化学(E-mail:657957680@qq.com)

通讯作者:
孙墨珑(1961-), 女, 博士, 教授, 研究方向为天然产物化学(E-mail:molongsun@126.com)

中图分类号: S792.132
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- 文章访问数: 954
- HTML全文浏览量: 134
- PDF下载量: 201
- 被引次数: 0
出版历程
- 收稿日期: 2017-03-29
- 修回日期: 2017-05-22
- 刊出日期: 2017-07-01

Process Optimization and Antioxidant Activity of Polyphenols Extracted from Leaves of Juglans mandshurica Maxim

College of Science, Northeast Forestry University, Harbin, Heilongjiang 150040, China

摘要

摘要: 采用核桃楸叶为原料，超声波辅助提取和紫外分光光度计对其多酚类物质进行含量测定。在单因素试验基础上，通过响应面法优化了核桃楸叶多酚类物质的提取工艺，得出核桃楸叶多酚类物质最佳提取条件为：乙醇体积分数70%，液料比15：1，超声时间30 min，超声温度60℃。核桃楸叶多酚类物质对羟基自由基、亚硝酸根离子、超氧阴离子、DPPH自由基有较强的清除能力，其对4种自由基的清除能力强弱顺序为DPPH自由基>超氧阴离子>羟基自由基>亚硝酸根离子，核桃楸叶多酚类物质对4种自由基的清除能力整体强于化学防腐剂苯甲酸，这表明核桃楸叶多酚类物质具有较强的抗氧化活性，是一种天然的抗氧化剂，为下一步研究多酚类物质的抗氧化性提供了依据。
- 核桃楸叶 /
- 响应面法 /
- 多酚 /
- 抗氧化
Abstract: Polyphenols were extracted from the leaves of Juglans mandshurica Maxim using an ultrasound-assisted process. Polyphenol content was determined by UV spectrophotometry. The extraction was optimized by a response surface experiment to arrive at the conditions including 70% by volume of ethanol as solvent, a solvent:solid ratio of 15:1, and an ultrasonic treatment of 30 min at 60℃. The polyphenol extract showed an excellent capacity in scavenging free radicals and ions in the order of DPPH radical > superoxide anion > hydroxyl radical > nitrite ion. It was more powerful than what the chemical preservative, such as benzoic acid, can provide. Thus, the polyphenols extracted from leaves of J. mandshurica by the current method could be a new natural antioxidant for potential applications.
- leaf of Juglans mandshurica Maxim /
- response surface methodology /
- polyphenol /
- antioxidant

HTML全文

图 1 乙醇体积分数、液料比、超声时间对多酚提取率影响的响应

Figure 1. Response surface diagram on yield of polyphenol affected by ethanol volume, solvent:solid ratio and time of extraction

下载: 全尺寸图片幻灯片

图 2 提取多酚抗氧化能力比较

Figure 2. Antioxidant capacity of polyphenol extract

下载: 全尺寸图片幻灯片

表 1 BBD试验设计因素与水平

Table 1. Levels and factors of Box-Behnken design

水平	酒精体积分数/%	液料比/(mL:g)	超声时间/min
-1	60	10:1	20
0	70	15:1	30
1	80	20:1	40

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表 2 BBD试验设计结果

Table 2. Results of data analysis on Box-Behnken experiment

序号	乙醇体积分数/%	液料比/(mL:g)	超声时间/min	多酚提取率/%
1	60	15:1	20	1.98
2	70	20:1	20	1.81
3	70	15:1	30	3.46
4	70	20:1	40	2.24
5	80	10:1	30	1.75
6	80	20:1	30	1.61
7	60	10:1	30	1.89
8	70	10:1	20	1.71
9	80	15:1	20	1.59
10	70	15:1	30	3.46
11	80	15:1	40	1.66
12	70	15:1	30	3.46
13	60	20:1	30	2.21
14	70	15:1	30	3.46
15	70	10:1	40	1.65
16	60	15:1	40	2.03
17	70	15:1	30	3.46

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表 3 多酚提取物对羟基自由基、亚硝酸根离子、超氧阴离子和DPPH自由基的清除率

Table 3. Scavenging capacities of polyphenol extract on hydroxyl radicals, nitrite ions, super-oxide anions and DPPH free radicals

样品浓度/(μg·mL^-1)	羟基自由基清除率/%			亚硝酸根离子清除率/%			超氧阴离子清除率/%			DPPH自由基清除率/%
样品浓度/(μg·mL^-1)	提取多酚	苯甲酸	抗坏血酸	提取多酚	苯甲酸	抗坏血酸	提取多酚	苯甲酸	抗坏血酸	提取多酚	苯甲酸	抗坏血酸
4	19.9	15.7	29.4	19.6	15.7	29.4	23.2	20.4	30.7	79.6	71.1	83.3
8	33.4	21.1	42.1	29.7	23.2	38.5	41.4	29.0	48.5	84.5	76.5	89.4
12	39.2	32.7	61.9	36.9	29.7	49.1	58.9	32.7	65.4	88.2	79.2	94.1
16	54.3	38.3	68.3	49.1	36.4	57.5	76.1	39.3	81.1	93.7	82.6	99.3
20	62.4	44.7	78.6	58.9	44.2	66.3	89.7	45.2	90.2	96.9	86.3	99.9

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