不同植物生长调节剂对复合盐胁迫下黑小麦种子萌发的影响

王泽琼; 蔡珺; 韩国良; 刘勇

doi:10.19303/j.issn.1008-0384.2019.03.016

不同植物生长调节剂对复合盐胁迫下黑小麦种子萌发的影响

doi: 10.19303/j.issn.1008-0384.2019.03.016

王泽琼^1,,
蔡珺¹,
韩国良¹,
刘勇^2, ,

1.
武汉生物工程学院, 湖北武汉 430415
2.
湖北省农业科学院果树茶叶研究所, 湖北武汉 430064

基金项目:

农业部华中作物有害生物综合治理重点实验室开放课题 2018ZTSJJ9

湖北省自然科学基金项目 2017CFB163

武汉市产学研项目 CXY201643

详细信息

作者简介:
王泽琼(1981-), 女, 博士, 副教授, 研究方向:植物生理学(E-mail:229941116@qq.com)

通讯作者:
刘勇(1981-), 男, 博士, 副研究员, 研究方向:植物生理学(E-mail:liuy1688@163.com)

中图分类号: S512.1
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- 被引次数: 0
出版历程
- 收稿日期: 2019-01-09
- 修回日期: 2019-02-25
- 刊出日期: 2019-03-28

Effects of Plant Growth Regulators on Germination of Black Wheat Seeds under Salt-stress

1.
Wuhan Institute of Bioengineering, Wuhan, Hubei 430415, China
2.
Institute of Fruit and Tea, Hubei Academy of Agricultural Science, Wuhan, Hubei 430064, China

摘要

摘要: 目的探讨α-萘乙酸钠（NAA）、赤霉素（GA₃）和6-苄氨基嘌呤（6-BA）3种植物生长调节剂不同质量浓度对不同程度盐胁迫条件下黑小麦种子萌发的影响，为在盐渍地区种植黑小麦提供可能性和科学依据。方法以紫麦一号的种子为试验材料，采用纸床法分别测定不同质量浓度的3种植物生长调节剂对不同程度盐胁迫条件下黑小麦种子萌发的影响。结果在无盐胁迫条件下，NAA、GA₃、6-BA质量浓度分别为150 mg·L^-1、GA₃ 200 mg·L^-1和6-BA 5 mg·L^-1时，黑小麦种子的发芽率、发芽势等萌发指标均为最佳；复合盐（NaCl：Na₂SO₄=1：1）溶液浸种显著抑制了黑小麦种子的萌发；用不同质量浓度的植物生长调节剂预处理均可在一定程度上缓解复合盐胁迫对黑小麦种子萌发的抑制作用；在中低浓度（40~80 mmol·L^-1）复合盐胁迫条件下，GA₃ 200 mg·L^-1和6-BA 5 mg·L^-1预处理均能有效缓解复合盐胁迫对种子萌发的影响；随着复合盐浓度的增加，各处理种子的发芽势和发芽率明显下降，当复合盐浓度为120 mmol·L^-1时，只有6-BA 5 mg·L^-1预处理对缓解胁迫有一定作用。结论在中低复合盐浓度胁迫条件下，采用GA₃（200 mg·L^-1）或6-BA（5 mg·L^-1）预处理能够缓解盐胁迫对黑小麦种子的伤害，从而显著促进在盐胁迫条件下黑小麦种子的萌发。
- 黑小麦种子 /
- 萌发 /
- 植物生长调节剂 /
- 复合盐 /
- 胁迫
Abstract: Objective Effects of 3 plant growth regulators on the germination of black wheat seeds under salt-stress were studied to provide possible relief for the wheat planting in saline-alkali areas. Method Using a paper bedding method, 1-N aphthaleneacetic acid (NAA), gibberellic acid (GA₃) or 6-benzylamino-purine (6-BA) was applied on seeds of black wheat (Zimai 1) to observe the germination under varied salt concentrations. Result The best rate, potential and other indices on the seed germination were found when 150 mg·L^-1 NAA, 200 mg·L^-1 GA₃ or 5 mg·L^-1 6-BA was used without salt-stress. The presence of NaCl:Na₂SO₄=1:1 significantly inhibited the germination. Addition of the plant growth regulators in different concentrations relieved the inhibitive effect to a certain extent. Both GA₃ at 200 mg·L^-1 and 6-BA at 5 mg·L^-1 significantly alleviated the germination inhibition when medium to low salt-stress (i.e., 40-80 mmol·L^-1) was imposed on the seeds. The germination tendency and rate were significantly decreased with increasing salt-stress. When the salt concentration was raised to 120 mmol·L^-1, only the application of 6-BA 5 mg·L^-1 exhibited an effect on the germination. Conclusion The treatment of GA₃ 200 mg·L^-1 or 6-BA 5 mg·L^-1 could protect the black wheat seeds from being permanently harmed by a medium-to-low salt-stress and improve the germination.
- black wheat seed /
- germination /
- hormone /
- compounded salt /
- stress

HTML全文

表 1 植物生长调节剂的质量浓度梯度设计

Table 1. Experimental design for applications of plant growth regulators in varied concentrations

植物生长调节剂 Plant growth regulator	质量浓度梯度 Concentration gradient/(mg·L^-1)
NAA	50	100	150	200	250
GA₃	100	150	200	250	300
6-BA	1	5	10	15	20

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表 2 NAA、GA₃、6-BA对黑小麦种子萌发的影响

Table 2. Effects of NAA, GA₃ and 6-BA on black wheat seed germination

植物生长调节剂 Plant growth regulator	浓度 Concentration / (mg·L^-1)	发芽率 Germination rate /%	发芽势 Germination energy/%	地上部长度 Above ground part/cm	根长 Length of root/cm
NAA	CK	72.67±4.17 Ccd	56.00±4.00 Cd	4.52±0.56 Bc	5.67±0.21 Aa
	50	70.00±4.00 Cd	58.67±4.16 Cd	8.13±0.57 Ab	2.15±0.25 Cd
	100	86.67±3.06 Aa	71.33±1.15 Bc	7.90±0.72 Ab	2.88±0.17 BCc
	150	87.33±2.31 Aa	85.33±4.17 Aa	9.27±0.67 Aa	3.50±0.20 Bb
	200	82.00±2.00 ABb	78.00±3.46 ABb	9.35±0.71 Aa	2.42±0.57 Ccd
	250	77.33±1.15 Bc	74.67±5.03 Bb	7.93±0.54 Ab	1.20±0.20 De
GA₃	CK	72.67±4.17 Bc	56.00±4.00 Cd	4.52±0.56 Cd	5.67±0.21 Dd
	100	91.33±5.03 Aa	82.67±2.31ABab	7.60±0.97 Bc	7.41±0.54 Cc
	150	90.00±5.29 Aa	84.33±2.04 ABb	9.71±0.36 Ab	7.52±0.41 BCc
	200	91.33±3.06 Aa	87.33±3.06 Aa	10.35±0.50 Aa	9.69±0.45 Aa
	250	78.67±1.15 Bb	76.25±3.06 Bb	10.26±0.62 Aa	8.35±0.16 Bb
	300	76.25±1.08 Bb	63.33±5.77 Cc	7.97±0.77 Bc	5.63±0.23 Dd
6-BA	CK	72.67±4.17 Bc	56.00±4.00 Cc	4.52±0.56 BCc	5.67±0.21 Aa
	1	88.67±3.06 Ab	86.02±1.84 Aa	5.51±0.03 ABb	3.46±0.47 Cc
	5	90.67±1.16 Aa	87.15±2.10 Aa	5.98±0.41 Aa	3.42±0.38 Cc
	10	86.00±2.00 Ab	84.00±2.00 Aa	4.96±0.48 ABCab	4.07±0.21 BCbc
	15	87.33±2.31 Ab	66.00±2.00 Bb	4.25±0.40 CDa	4.60±0.26 Bb
	20	84.67±4.16 Ab	70.00±4.00 Bb	3.69±0.29 Dd	3.53±0.42 Cb
注：同列数据中不同大、小写字母分别表示在0.01、0.05水平上的差异显著性(Duncan′s法)。 Note: Data with different capital and lowercase letters on a same column represent extremely significant difference at 0.01 and significant difference at 0.05 level，respectively (Duncan′s).

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表 3 植物生长调节剂对盐胁迫抑制黑小麦种子萌发的缓解效果

Table 3. Relief effect of plant growth regulators on black wheat seed germination inhibited by salt-stress

复合盐浓度 Concentration of complex salt / (mmol·L^-1)	植物生长调节剂 Plant growth regulator	发芽率 Germination rate /%	发芽势 Germination energy /%	相对盐害率 Relative injury rate /%	地上部长度 Above ground part/cm	根长 Length of root/cm
0(CK)	无	72.67±4.17	56.00±4.00	0	4.52±0.56	5.67±0.21
40	无	56.00±2.00 Cc	18.00±3.46 Cc	22.94	4.35±0.43 BCc	4.54±0.25 Bb
	NAA	64.00±4.00 Bb	18.00±2.00 Cc	-	4.04±0.30 Cc	3.24±0.19 Cc
	GA₃	87.33±3.06 Aa	34.00±3.46 Bb	-	7.31±0.27 Aa	8.43±0.36 Aa
	6-BA	85.33±1.15 Aa	60.00±5.29 Aa	-	5.02±0.20 Bb	3.31±0.36 Cc
80	无	21.33±4.16 Cd	4.00±2.00 Bc	70.65	1.84±0.28 Cd	2.32±0.24 Bc
	NAA	30.00±4.00 Bc	0±0.00 Bd	-	2.00±0.22 Cc	2.25±0.27 Bc
	GA₃	44.00±4.00 Ab	13.33±3.06 Ab	-	5.91±0.47 Aa	5.34±0.55 Aa
	6-BA	54.67±5.03 Aa	19.33±4.16 Aa	-	4.93±0.27 Bb	2.77±0.25 Bb
120	无	14.00±1.25 Bc	4.00±0.00 Bb	80.73	1.63±0.25 Cc	1.56±0.30 Bc
	NAA	14.00±3.46 Bc	0±0.00 Cc	-	1.35±0.12 Cc	1.32±0.29 Bc
	GA₃	20.67±3.06 Bb	14.00±4.00 Aa	-	2.67±0.32 Bb	3.37±0.23 Aa
	6-BA	44.00±2.00 Aa	10.67±3.06 ABa	-	3.93 ±0.32 Aa	2.02±0.16 Bb
160	无	4.00±2.00 Bb	4.00±2.00 ABab	94.50	0.67±0.17 Bc	0.91±0.06 Bc
	NAA	3.33±1.15 Bb	0±0.00 Bc	-	0.92±0.15 Bb	1.06±0.11 Bc
	GA₃	11.33±3.06 Aa	6.00±2.00 Aa	-	0.72±0.10 Bc	1.83±0.25 Aa
	6-BA	12.00±3.46 Aa	1.33±1.15 Bb	-	1.33±0.11 Aa	1.41±0.22 ABb
200	无	0	0	100	0	0
注：(1)同列数据中不同大、小写字母分别表示在0.01、0.05水平上的差异显著性(Duncan′s法)。(2)NAA、GA₃、6-BA的质量浓度分别为150 mg·L^-1、200 mg·L^-1、5 mg·L^-1。 Note: (1) Data with different capital and lowercase letters on a same column represent extremely significant difference at 0.01 and significant difference at 0.05 level，respectively (Duncan′s). (2) Concentrations of NAA，GA₃ and 6-BA are 150 mg·L^-1，200 mg·L^-1 and 5 mg·L^-1，respectively.

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