Effect of Light Intensity on Growth and Physiological Properties of Free-living Conchocelis of Pyropia haitanensis
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摘要:
目的 探讨坛紫菜自由丝状体营养藻丝快速生长的最适宜光照强度。 方法 以坛紫菜新品种申福2号自由丝状体营养藻丝为培养材料，研究不同光照强度（10、20、40、80、100 μmol·photons m-2·s-1）对自由丝状体营养藻丝生长、光系统PSⅡ最大量子效率、光合色素含量、光合作用及抗氧化酶活性的影响。 结果 经25 d培养后，坛紫菜自由丝状体营养藻丝的增重随光照强度梯度增加而表现出先增加后降低的趋势，其中80 μmol·photons m-2·s-1光照强度培养下的藻体增重最多，且光系统PSⅡ最大量子效率Fv/Fm值最高。叶绿素a、类胡萝卜素、藻红蛋白含量整体上随光强的增加而逐渐降低，藻蓝蛋白含量则在各组间差异不显著。藻体CA和RubisCO酶活性均随光强增加而呈现先增强后减弱的趋势。其中，以40 μmol·photons m-2·s-1培养下的酶活性最强，80 μmol·photons m-2·s-1次之。超氧阴离子自由基和SOD在不同光强下的活性随光强增加呈现先减弱后增强的趋势，80 μmol·photons m-2·s-1下培养的坛紫菜自由丝状体营养藻丝这两种酶的活性均最低，分别为最高活性试验组的24.0%和22.9%。 结论 在80 μmol·photons m-2·s-1培养下，坛紫菜自由丝状体营养藻丝增重最多，藻体最大量子效率Fv/Fm值最大，RubisCO和CA活性较强，超氧阴离子和SOD活性最低，该光照强度最有利于坛紫菜自由丝状体营养藻丝的快速增殖。 Abstract:Objective Intensity of LED light for optimal growth of free-living conchocelis of Pyropia haitanensis (FCP) was studied. Method The effects of varied light intensities (i.e., 10, 20, 40, 80 or 100 μmol·photons m-2·s-1) using LED on the growth, maximal quantum efficiency (Fv/Fm) on PS Ⅱ, content of photosynthetic pigments as well as the photosynthesis and antioxidant enzymes of Shenfu No.2, a new cultivar of P. haitanensis, were determined. Result After 25 d of culturing, the weight of algae increased with increasing intensity of illumination and peaked when 80 μmol·photons m-2·s-1 was applied. As the weight reached the maximum, the Fv/Fm on PS Ⅱ was also at its highest level. On the other hand, the contents of chlorophyll a, carotenoids, and phycoerythrin decreased gradually as the light intensity increased. No significant differences on phycocyanin among the groups were observed. The greatest activities of CA and RubisCO in the algae were observed under 40 μmol·photons m-2·s-1 followed by 80 μmol·photons m-2·s-1 treatment before a decline. Upon an increase on light intensity, the activities of superoxide anion radicals and SOD decreased to the lowest level under 80 μmol·photons m-2·s-1 and then raised again. Conclusion The vegetative filaments of FCP cultured under 80 μmol·photons m-2·s-1 light appeared to render the highest algae growth rate and Fv/Fm, lowest activities of superoxide anions and SOD, and strongest activities of RubisCO and CA. -
Key words:
- Pyropia haitanensis /
- free-living conchocelis /
- light intensity /
- growth /
- physiological characteristics
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图 3 不同光强培养下坛紫菜自由丝状体营养藻丝的藻胆蛋白含量
Figure 3. Contents of phycobiliproteins in vegetative filaments of FCP grown under different light intensities
图 4 不同光强培养条件下坛紫菜自由丝状体营养藻丝的Fv/Fm
Figure 4. Fv/Fm of FCP grown under different light intensities
图 5 不同光强下坛紫菜自由丝状体营养藻丝的RubisCO和CA活性
Figure 5. RubisCO and CA activities of FCP grown under different light intensities
图 6 不同光强下坛紫菜自由丝状体营养藻丝的超氧阴离子自由基和SOD活性
Figure 6. Superoxide anion radical and SOD activities of FCP grown under different light intensities
表 1 坛紫菜自由丝状体营养藻丝在不同光强下的特定生长率
Table 1. SGR of FCP grown under different light intensities
培养时间
Culture time/d光照强度
Light intensity/(μmol·photons m-2·s-1)10 20 40 80 100 0~5 14.69 17.65 14.94 14.98 13.14 6~10 4.14 2.85 8.30 6.87 6.99 11~15 2.14 4.11 2.92 4.75 3.77 16~20 0.96 2.69 2.13 7.01 3.72 21~25 1.26 2.37 4.56 3.50 4.96 
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