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嘉宝果叶片总多酚提取工艺优化及其体外降糖活性

林宝妹 邱珊莲 郑开斌 张帅 洪佳敏 张少平

林宝妹, 邱珊莲, 郑开斌, 张帅, 洪佳敏, 张少平. 嘉宝果叶片总多酚提取工艺优化及其体外降糖活性[J]. 福建农业学报, 2019, 34(5): 587-594. doi: 10.19303/j.issn.1008-0384.2019.05.012
引用本文: 林宝妹, 邱珊莲, 郑开斌, 张帅, 洪佳敏, 张少平. 嘉宝果叶片总多酚提取工艺优化及其体外降糖活性[J]. 福建农业学报, 2019, 34(5): 587-594. doi: 10.19303/j.issn.1008-0384.2019.05.012
LIN Bao-mei, QIU Shan-lian, ZHENG Kai-bin, ZHANG Shuai, HONG Jia-min, ZHANG Shao-ping. Process Optimization and in vitro Hypoglycemic Effect of Polyphenols Extracted from Jaboticaba Leaves[J]. Fujian Journal of Agricultural Sciences, 2019, 34(5): 587-594. doi: 10.19303/j.issn.1008-0384.2019.05.012
Citation: LIN Bao-mei, QIU Shan-lian, ZHENG Kai-bin, ZHANG Shuai, HONG Jia-min, ZHANG Shao-ping. Process Optimization and in vitro Hypoglycemic Effect of Polyphenols Extracted from Jaboticaba Leaves[J]. Fujian Journal of Agricultural Sciences, 2019, 34(5): 587-594. doi: 10.19303/j.issn.1008-0384.2019.05.012

嘉宝果叶片总多酚提取工艺优化及其体外降糖活性

doi: 10.19303/j.issn.1008-0384.2019.05.012
基金项目: 

福建省科技计划项目——省属公益类科研院所基本科研专项 2018R1024-6

福建省农业科学院科技创新项目 PC2018-9-6

福建省农业科学院青年英才计划项目 YC2015-19

福建省农业科学院青年创新团队项目 STIT2017-3-4

福建省农业科学院生产性工程化实验室中试项目 AG2017-5

详细信息
    作者简介:

    林宝妹(1988-), 女, 硕士, 研究实习员, 研究方向:天然产物化学(E-mail:yogobm@qq.com)

    通讯作者:

    邱珊莲(1979-), 女, 博士, 副研究员, 研究方向:天然产物化学(E-mail:slqiu79@163.com)

  • 中图分类号: Q946.8

Process Optimization and in vitro Hypoglycemic Effect of Polyphenols Extracted from Jaboticaba Leaves

  • 摘要:   目的  优化嘉宝果叶片总多酚的提取工艺,检测优化提取后的嘉宝果叶片提取物对α-葡萄糖苷酶的抑制作用。  方法  利用超声波辅助与乙醇提取相结合的方法,以总多酚提取量为考察指标,采用单因素及正交试验,研究功率、时间、乙醇体积分数、料液比和温度对嘉宝果叶片总多酚提取量的影响,并采用酶动力学及紫外可见吸收光谱法探讨叶片提取物体外抑制α-葡萄糖苷酶的活性。  结果  嘉宝果叶片总多酚的最佳提取工艺为:功率120 W,时间10 min,乙醇体积分数40%,料液比1:160(g:mL),温度40℃,在此条件下,嘉宝果叶片总多酚质量分数可达33.84%。优化提取后的嘉宝果叶片提取物对α-葡萄糖苷酶具有较强的抑制作用,半数抑制浓度IC50为0.003 1 mg·mL-1,阿卡波糖对α-葡萄糖苷酶的半数抑制浓度IC50为8.110 8 mg·mL-1;Lineweaver-Burk双倒数作图显示嘉宝果叶片多酚对α-葡萄糖苷酶的抑制类型为反竞争性抑制。  结论  正交实验法优化嘉宝果叶片总多酚提取具有可行性,嘉宝果叶片总多酚对α-葡萄糖苷酶具有较强的抑制作用,可作为降血糖功能因子进行开发利用。
  • 图  1  功率对总多酚提取量的影响

    Figure  1.  Effect of ultrasonic wave power on polyphenol extraction

    图  2  时间对总多酚提取量的影响

    Figure  2.  Effect of ultrasonic wave application duration on polyphenol extraction

    图  3  乙醇体积分数对总多酚提取量的影响

    Figure  3.  Effect of ethanol volume fraction on polyphenol extraction

    图  4  料液比对总多酚提取量的影响

    Figure  4.  Effect of material-solvent ratio on polyphenol extraction

    图  5  温度对总多酚提取量的影响

    Figure  5.  Effect of processing temperature on polyphenol extraction

    图  6  嘉宝果叶片总多酚提取物(A)及阿卡波糖(B)对α-葡萄糖苷酶的抑制作用

    Figure  6.  Effects of polyphenol extract (A) and acarbose (B) on α-glucosidase

    图  7  嘉宝果叶片总多酚提取物对α-葡萄糖苷酶抑制作用类型

    Figure  7.  Inhibition type of polyphenol extract on α-glucosidase

    图  8  嘉宝果叶片总多酚提取物对α-葡萄糖苷酶抑制作用的Lineweaver-Burk曲线

    Figure  8.  Lineweaver-Burk plot on inhibition of polyphenol extract on α-glucosidase

    表  1  正交试验因素水平

    Table  1.   Factors and levels of orthogonal experiment

    水平
    Level
    乙醇体积分数
    Ethanol volume fraction/%
    料液比
    Material-solvent ratio/(g:mL)
    温度
    Temperature/℃
    1 20 1:120 20
    2 40 1:160 30
    3 60 1:200 40
    下载: 导出CSV

    表  2  正交试验设计与结果

    Table  2.   Orthogonal design, results and range analysis

    试验号
    Numbers
    因素 总多酚提取量
    Total polyphenol/%
    A/% B/(g:mL) C/℃ D
    1 1(20) 1(1:120) 1(20) 1 30.13
    2 1 2(1:160) 2(30) 2 31.91
    3 1 3(1:200) 3(40) 3 31.59
    4 2(40) 1 2 3 32.67
    5 2 2 3 1 34.52
    6 2 3 1 2 34.06
    7 3(60) 1 3 2 32.22
    8 3 2 1 3 33.14
    9 3 3 2 1 33.20
    k1 93.63 95.02 97.33 97.85
    k2 101.25 99.57 97.78 98.19
    k3 98.56 98.85 98.33 97.40
    R 7.62 4.55 1.00 0.79
    注:表中因素A为乙醇体积分数,B为液料比,C为温度,D为空列。表 3同。
    Note:A.Ethanol volume fraction,B.Material-solvent ratio,C.Temperature,D.Vacant column.The same as table 3.
    下载: 导出CSV

    表  3  正交试验结果方差分析

    Table  3.   Analysis of variance on results of orthogonal experiment

    变异来源
    Variations
    偏差平方和
    Sum of square
    自由度
    Freedom
    F
    F value
    F临界值
    F critical value
    P
    P value
    A 9.956 2 94.819 19.000 <0.05
    B 3.988 2 37.981 19.000 <0.05
    C 0.167 2 1.590 19.000 >0.05
    D 0.105 2 1.000 19.000 >0.05
    误差Errors 0.105 2
    下载: 导出CSV

    表  4  嘉宝果总多酚提取物对α-葡萄糖苷酶抑制作用的Lineweaver-Burk曲线方程

    Table  4.   Lineweaver-Burk plot equation for inhibition of polyphenol extract on α-glucosidase

    项目
    Items
    质量浓度
    Mass concentration/(mg·mL-1)
    线性回归方程Linear regression equation R2 Vmax/(OD·min-1) Km/(mmol·L-1)
    斜率Slope 截距Intercept
    对照组Contrast 0 5.7203 8.0232 0.9976 0.1246 0.7130
    嘉宝果总多酚
    Total polyphenol
    0.003 5.8844 22.6842 0.9974 0.0441 0.2594
    0.006 5.8105 61.6144 0.9976 0.0162 0.0943
    下载: 导出CSV
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  • 收稿日期:  2018-12-25
  • 修回日期:  2019-03-12
  • 刊出日期:  2019-05-28

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