新白8号枇杷果肉香气成分及HS-SPME内标浓度分析

张桂彬; 安宁; 朱立辉; 蒋际谋; 胡文舜

doi:10.19303/j.issn.1008-0384.2019.06.006

新白8号枇杷果肉香气成分及HS-SPME内标浓度分析

doi: 10.19303/j.issn.1008-0384.2019.06.006

张桂彬¹,
安宁¹,
朱立辉¹,
蒋际谋^2, ,,
胡文舜^{1, 2, ,}

1.
福建农林大学园艺学院, 福建福州 350002
2.
福建省农业科学院果树研究所, 福建福州 350013

基金项目:

福建省财政专项——福建省农业科学院科技创新团队建设项目 STIT2017-1-4

福建省科技星火计划项目 217S0009

详细信息

作者简介:
张桂彬(1998-), 女, 主要从事枇杷果实品质方面研究

通讯作者:
蒋际谋(1974-), 男, 研究员, 主要从事果树育种、种质资源和配套技术研究(E-mail:jjm2516@126.com)

胡文舜(1984-), 男, 硕士, 助理研究员, 主要从事龙眼、枇杷遗传育种与分子生物学研究(E-mail:huwenshun06@163.com)

中图分类号: S667.3
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- 被引次数: 0
出版历程
- 收稿日期: 2019-02-12
- 修回日期: 2019-05-14
- 刊出日期: 2019-06-28

Aromatics Determination of Xinbai 8 Loquat by HS-SPME Internal Standard Method

ZHANG Gui-bin¹,
AN Ning¹,
ZHU Li-hui¹,
JIANG Ji-mou^{2
, ,},
HU Wen-shun^{1, 2
, ,}

1.
Horticultural College, Fujian Agricultural and Forestry University, Fuzhou, Fujian 350002, China
2.
Institute of Fruit Trees, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350002, China

摘要

摘要: 目的探究新白8号枇杷香气成分及内标物的最适浓度，以期为白肉枇杷香气成分的研究及内标选择提供依据。方法以白肉枇杷新白8号果实为试材，加入4种不同浓度等体积的环己酮溶液（947、94.7、9.47、0.947μg·μL^-1）为内标，采用HS-SPME-GC/MS技术，对试材的香气成分进行定性和定量分析。结果新白8号枇杷果肉中共检测出10类43种香气成分，以醛类、烷烃、芳香烃、酮类和醇类为主，其中相对含量较高的物质有己醛、（E）-2-己烯醛、正丁基环己烷、2-庚酮、正戊基环己烷、（E）-2-己烯-1-醇，苯乙烯等。4种环己酮浓度的样品分别检测出有效香气化合物为30、39、41和32种，有效峰面积占总峰面积比分别为38.20%、28.49%、42.70%和39.51%；环己酮峰面积比分别为47.38%、58.55%、36.37%和25.86%。加入9.47 μg·μL^-1环己酮的样品检测出的香气物质种类最多、有效峰面积比最高、环己酮及各香气成分峰面积占比适宜。结论新白8号香气成分丰富多样，9.47 μg·μL^-1环己酮为枇杷果实香气成分固相微萃取的适宜内标浓度。
- 枇杷 /
- 香气 /
- 环己酮 /
- 内标 /
- 固相微萃取 /
- 气相色谱-质谱
Abstract: Objective To determine the aromatic composition of loquat fruit and cyclohexanone concentration for the HS-SPME-GC/MS internal standard method. Methods The pulp of Xinbai 8 fruits was added with a cyclohexanone solution in the concentration of 947, 94.7, 9.47, 0.947 μg·μL^-1 at a same volume as the internal standard for HS-SPME-GC/MS analysis. Results The analysis detected 10 classifications of chemicals including aldehydes, alkanes, dutrex, ketones and alcohols. Hexanal, (E)-2-hexenal, n-butyl cyclohexane, 2-heptanone, n-pentyl cyclohexane, (E)-2-hexene-1-alcohol, and styrene were the dominating volatiles among the 43 compounds identified.In the cyclohexanone-added samples, the decreasing concentration produced 31, 40, 42 and 33 aromatic substances with the effective peak area ratios of 38.20%, 28.49%, 42.70% and 39.52%, and the peak area ratios of 38.20%, 58.55%, 36.37% and 25.86%, respectively. A maximum number of aromatics was found in the sample that was added with 9.47 μg·μL^-1 cyclohexanone which also had the highest effective peak area ratio and optimum peak area radio. Conclusion The aromatics in the fruits of Xinbai 8 were diverse, and the cyclohexanone concentration at 9.47 μg·μL^-1 was the choice internal standard for the analytical.
- loquat /
- aromatics /
- cyclohexanone /
- internal standard /
- SPME /
- GC-MS

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图 1 枇杷果实香气成分4种环己酮浓度的总离子色谱

Figure 1. Total ion chromatograms of aromatics in loquat fruits using cyclohexanone at 4 concentrations

下载: 全尺寸图片幻灯片

图 2 4种环己酮浓度下枇杷各类别香气成分的峰面积比及数量

Figure 2. Number and area ratio of peaks for various aroma types using 4 cyclohexanone concentrations as internal standard

下载: 全尺寸图片幻灯片

表 1 新白8号果实香气主要成分的相对含量

Table 1. Relative content of aromatics in fruits of Xinbai 8

编号 No.	化学成分 Components	相对含量Relative content/(ng·g^-1 FW)
编号 No.	化学成分 Components	×1环己酮 Cyclohexanone	×10环己酮 Cyclohexanone	×100环己酮 Cyclohexanone	×1000环己酮 Cyclohexanone
	酯类Esters
1	2-甲基丁酸甲酯methyl 2-methylbutyrate	－	－	－	0.10
2	甲酸环己酯Formic acid, cyclohexyl ester	901.57	84.85	18.76	－
3	乙酸环己酯Acetic acid, cyclohexyl ester	－	3.37	0.56	－
4	顺式-3-己烯醇乳酸酯cis-3-Hexenyllactate	36.35	－	－	－
	醇类Alcohol
5	1-甲基环己醇1-MethyLcyclohexanol	339.30	49.95	6.77	0.35
6	正己醇1-Hexano	178.37	85.49	17.66	2.94
7	(Z)-3-己烯醇3-Hexen-1-ol, (E)-	52.04	22.05	4.55	0.52
8	(E)-2-己烯-1-醇2-Hexen-1-ol, (E)-	－	141.39	34.64	5.32
9	2-乙基己醇1-Hexanol, 2-ethyl-	57.17	8.45	2.08	0.36
10	正辛醇1-Octanol	48.93	11.68	2.10	0.34
11	新薄荷醇(1R)-(+)-neomenthol	－	－	5.28	－
12	戊乙二醇Pentaethylene glycol	98.08	38.10	0.78	0.20
	酮类Ketones
13	3-庚酮3-Heptanone	331.77	38.12	5.44	－
14	2-庚酮2-Heptanone	8144.71	503.82	65.1	1.93
15	环戊基乙酮Ethanone, 1-cyclopentyl-	96.90	10.76	1.49	－
16	2-甲基环戊酮Cyclohexanone, 2-methyL-	634.71	72.03	8.41	－
17	甲基庚烯酮5-Hepten-2-one, 6-methyL-	78.23	16.85	2.98	0.34
18	2-环己烯酮2-CycLohexen-1-one	415.65	22.48	3.48	0.51
19	苯乙酮Acetophenone	－	3.50	0.52	0.14
20	1.4-环己二酮2-Cyclohexene-1, 4-dione	－	－	1.10	0.23
21	香叶基丙酮Geranylacetone	－	12.46	2.60	－
22	β-紫罗酮Beta-Ionone	168.23	12.12	2.47	0.69
	烷烃Alkane
23	正丁基环己烷Cyclohexane, butyl-	19873.41	278.97	156.65	0.48
24	正戊基环己烷Cyclohexane, pentyl-	15890.51	207.81	104.70	0.93
25	环十二烷Cyclododecane	－	4.38	0.70	0.18
	醛类Aldehyde
26	己醛Hexanal	4908.29	1295.02	301.00	74.94
27	(E)-2-己烯醛2-HexenaL, (E)-	7896.42	1569.74	382.22	55.98
28	环己基甲醛Cyclohexane carboxaldehyde	111.95	8.48	1.86	－
29	壬醛Nonanal	228.65	34.57	6.01	1.12
30	(E.E)-2.4-庚二烯醛2, 4-Hexadienal, (E, E)-	580.28	23.18	6.58	1.12
31	癸醛Decanal	126.22	16.45	2.37	0.70
32	苯甲醛Benzaldehyde	148.86	10.08	2.54	0.51
33	β-环柠檬醛Beta-cyclocitral	－	5.92	0.97	0.22
	酸类Acid
34	乙酸Acetic acid	70.98	4.96	1.11	0.28
35	2-甲基丁酸Butanoic acid, 2-methyl-	－	4.72	1.37	0.35
36	己酸Hexanoic acid	201.78	20.25	5.81	1.50
37	(E)-2-己烯酸2-Hexenoic acid, (E)-	－	30.07	5.74	1.71
	酚类Phenol
38	2, 6-二叔丁基对甲酚Butylated hydroxytoluene	－	43.68	5.89	2.89
	醚类Ether
39	三乙二醇单乙醚Ethanol, 2-(2-ethoxyethoxy)-	398.08	4.19	9.78	2.20
	芳香烃Aromatic hydrocarbon
40	苯乙烯Styrene	3442.55	111.46	20.67	0.62
41	乙苯Ethylbenzene	2264.31	43.77	14.90	－
42	对二甲苯p-Xylene	17089.28	249.82	15.61	－
	其他Others
43	2-乙基呋喃Furan, 2-ethyl-	－	15.09	1.97	0.11
	相对总含量The relative total content	84813.58	5120.08	1235.22	160.81
	累计有效峰面积All of effective peak area	7.37×10⁹	4.18×10⁹	3.99×10⁹	1.58×10⁹
	有效峰面积/总峰面积	38.20%	28.49%	42.70%	39.51%
	The proportion of effective and total peak area
	环己酮峰面积The peak area of cyclohexanone	9.14×10⁹	8.58×10⁹	3.40×10⁹	1.06×10⁹
注：“-”表示未检测到。 Note：“-” indicates undetected.

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