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不同种植模式七叶一枝花土壤芽胞杆菌多样性研究

苏海兰 郑梅霞 朱育菁 单寄坪

苏海兰, 郑梅霞, 朱育菁, 单寄坪. 不同种植模式七叶一枝花土壤芽胞杆菌多样性研究[J]. 福建农业学报, 2019, 34(8): 974-984. doi: 10.19303/j.issn.1008-0384.2019.08.016
引用本文: 苏海兰, 郑梅霞, 朱育菁, 单寄坪. 不同种植模式七叶一枝花土壤芽胞杆菌多样性研究[J]. 福建农业学报, 2019, 34(8): 974-984. doi: 10.19303/j.issn.1008-0384.2019.08.016
SU Hai-lan, ZHENG Mei-xia, ZHU Yu-jing, SHAN Ji-ping. Bacillus spp. in Soils at Paris polyphylla Cultivation Fields[J]. Fujian Journal of Agricultural Sciences, 2019, 34(8): 974-984. doi: 10.19303/j.issn.1008-0384.2019.08.016
Citation: SU Hai-lan, ZHENG Mei-xia, ZHU Yu-jing, SHAN Ji-ping. Bacillus spp. in Soils at Paris polyphylla Cultivation Fields[J]. Fujian Journal of Agricultural Sciences, 2019, 34(8): 974-984. doi: 10.19303/j.issn.1008-0384.2019.08.016

不同种植模式七叶一枝花土壤芽胞杆菌多样性研究

doi: 10.19303/j.issn.1008-0384.2019.08.016
基金项目: 

福建省科技计划项目——省属公益类科研院所基本科研专项 2019R11020019-9

福建省财政专项——福建省农业科学院创新团队建设项目 STIT2017-2-8

福建省农业科学院科技服务团队项目 kjfw20

详细信息
    作者简介:

    苏海兰(1980-), 女, 硕士, 农艺师, 研究方向:药用植物资源利用与栽培研究(E-mail:363801575@qq.com)

    通讯作者:

    朱育菁(1972-), 女, 博士, 研究员, 研究方向:农业生物资源与生物防治的研究(E-mail:zyjingfz@163.com)

  • 中图分类号: Q938

Bacillus spp. in Soils at Paris polyphylla Cultivation Fields

  • 摘要:   目的  分析水稻田、山垅和林下3种种植模式七叶一枝花土壤中芽胞杆菌资源多样性,为七叶一枝花的栽培提供理论基础。  方法  采用五点采样法,分别采集水稻田、山垅田与林下种植模式七叶一枝花根际土壤与非根际土壤样本6份,采用涂布法分离芽胞杆菌,采用16S rRNA基因同源性将其鉴定。  结果  一共分离获得了87株芽胞杆菌,13个属、35个种,分别属于芽胞杆菌属Bacillus的16个种、赖氨酸芽胞杆菌属Lysinibacillus、类芽胞杆菌属Paenibacillus和假单胞菌属Pseudomonas的各3个种、Bhargavaea、短杆菌属BrevibacteriumBurkholderia、短芽胞杆菌属Brevibacillus、代夫特菌属DelftiaPaenarthrobacter、节杆菌属Arthrobacter、伯克氏菌属泛菌属Pantoea、假单胞菌属Pseudomonas的各1个种。根据分离频度分析得知,七叶一枝花土壤中的芽胞杆菌优势菌群为图瓦永芽胞杆菌B.toyonensis和假蕈状芽胞杆菌B.pseudomycoides。其中,水稻田种植模式土壤中的芽胞杆菌的数量最多,且Margalef、Shannon-Wiener、Pielou和Simpson多样性指数都最高。七叶一枝花根际土壤中的芽胞杆菌数量均大于非根际土壤。  结论  水稻田蕴藏着丰富的芽胞杆菌种类和数量,多样性高,在七叶一枝花的种植中具有很好的应用前景。
  • 图  1  芽胞杆菌的菌落形态

    Figure  1.  Colony morphology of Bacillus spp.

    图  2  基于16S rRNA基因序列的南平七叶一枝花不同种植模式土壤中芽胞杆菌系统发育树

    注:数字表示各节点自发展支持率数值(>50)。

    Figure  2.  Phylogenetic tree of Bacillus spp. isolated from soil at P. polyphylla cultivation fields in Nanping based on 16S rRNA gene sequences using neighbor-joining method

    Note:Data at branching points indicate bootstrap values are greater than 50%.

    图  3  不同种植模式七叶一枝花土壤芽胞杆菌的多样性指数

    注:Ⅰ为水稻田,Ⅱ为山垅田,Ⅲ为林下。

    Figure  3.  Diversity indices of Bacilus spp. isolated from soil at paddy field (SDT), mountain ridge (SLT) and understory (LX) growing P. polyphylla

    Note:Ⅰ.SDT, Ⅱ.SLT, Ⅲ.LX.

    表  1  从七叶一枝花不同种植模式土壤中分离得到的细菌的种类

    Table  1.   Bacillus spp. isolated from soil at P. polyphylla cultivation fields

    分离来源
    Reource
    类群
    Group
    代表菌株
    Strains
    最相近菌株
    Closest match
    相似性
    Sequence identify/%
    水稻田非根际土(SDF)
    Irrhizosphere soil in paddy field
    芽胞杆菌属 Bacillus FJAT-49195 高地芽胞杆菌 Bacillus altitudinis 100.00
    FJAT-49204 高地芽胞杆菌 Bacillus altitudinis 100.00
    FJAT-49198 钻特省芽胞杆菌 Bacillus drentensis 99.64
    FJAT-49200 太平洋芽胞杆菌 Bacillus pacificus 100.00
    FJAT-49201 阿氏芽胞杆菌 Bacillus aryabhattai 100.00
    FJAT-49211 图瓦永芽胞杆菌 Bacillus toyonensis 99.93
    FJAT-49206 阿氏芽胞杆菌 Bacillus aryabhattai 100.00
    FJAT-49207 扁平芽胞杆菌 Bacillus depressus 99.15
    FJAT-49196 假蕈状芽胞杆菌 Bacillus pseudomycoides 98.87
    FJAT-49208 Bacillus pseudomycoides 100.00
    FJAT-49209 假蕈状芽胞杆菌 Bacillus pseudomycoides 97.95
    赖氨酸芽胞杆菌属 Lysinibacillus FJAT-49203 Lysinibacillus fusiformis 99.72
    FJAT-49202 Lysinibacillus fusiformis 99.72
    FJAT-49199 Lysinibacillus mangiferihumi 99.93
    短芽胞杆菌 Brevibacillus FJAT-49197 耐寒短杆菌 Brevibacterium frigoritolerans 99.79
    Paenarthrobacter FJAT-49205 Paenarthrobacter ilicis 99.57
    类芽胞杆菌属 Paenibacillus FJAT-49210 Paenibacillus silvae 99.50
    水稻田根际土
    (SDG)Rhizosphere soil in paddy field
    芽胞杆菌属 Bacillus FJAT-49212 图瓦永芽胞杆菌 Bacillus toyonensis 99.79
    FJAT-49218 图瓦永芽胞杆菌 Bacillus toyonensis 100.00
    FJAT-49223 图瓦永芽胞杆菌 Bacillus toyonensis 100.00
    FJAT-49225 Bacillus pseudomycoides 100.00
    FJAT-49219 假蕈状芽胞杆菌 Bacillus pseudomycoides 99.79
    FJAT-49214 阿氏芽胞杆菌 Bacillus aryabhattai 100.00
    FJAT-49215 高地芽胞杆菌 Bacillus altitudinis 100.00
    FJAT-49216 Bacillus albus 99.65
    FJAT-49213 泰门芽胞杆菌 Bacillus timonensis 98.94
    FJAT-49224 钻特省芽胞杆菌 Bacillus drentensis 99.49
    FJAT-49220 Bacillus mobilis 99.79
    FJAT-49227 Bacillus mobilis 100.00
    FJAT-49228 科研中心芽胞杆菌 Bacillus cecembensis 97.19
    FJAT-49229 扁平芽胞杆菌 Bacillus depressus 98.65
    类芽胞杆菌属 Paenibacillus FJAT-49222 乐金类芽胞杆菌 Paenibacillus elgii 99.64
    FJAT-49226 蜂房类芽胞杆菌 Paenibacillus alvei 99.09
    赖氨酸芽胞杆菌属 Lysinibacillus FJAT-49217 解木糖赖氨酸芽胞杆菌 Lysinibacillus xylanilyticus 99.72
    FJAT-49221 纺锤形赖氨酸芽胞杆菌 Lysinibacillus fusiformis 100.00
    林下非根际土
    (LXF) Irrhizosphere soil in understory
    芽胞杆菌属 Bacillus FJAT-49230 Bacillus proteolyticus 99.93
    FJAT-49238 Bacillus proteolyticus 99.86
    FJAT-49237 图瓦永芽胞杆菌 Bacillus toyonensis 100.00
    FJAT-49240 图瓦永芽胞杆菌 Bacillus toyonensis 99.93
    FJAT-49231 Bacillus paramycoides 100.00
    代夫特菌 Delftia FJAT-49233 湖生代夫特菌 Delftia lacustris 99.86
    赖氨酸芽胞杆菌属 Lysinibacillus FJAT-49232 解木糖赖氨酸芽胞杆菌 Lysinibacillus xylanilyticus 97.96
    FJAT-49234 解木糖赖氨酸芽胞杆菌 Lysinibacillus xylanilyticus 99.58
    FJAT-49235 解木糖赖氨酸芽胞杆菌 Lysinibacillus xylanilyticus 98.85
    类芽胞杆菌属 Paenibacillus FJAT-49236 蜂房类芽胞杆菌 Paenibacillus alvei 99.02
    短芽胞杆菌 Brevibacillus FJAT-49239 侧胞短芽胞杆菌 Brevibacillus laterosporus 99.57
    伯克霍尔德菌 Burkholderia FJAT-49241 稳定伯克霍尔德菌 Burkholderia stabilis 99.79
    林下根际土(LXG)
    Rhizosphere soil in understory
    芽胞杆菌属 Bacillus FJAT-49242 图瓦永芽胞杆菌 Bacillus toyonensis 99.93
    FJAT-49243 图瓦永芽胞杆菌 Bacillus toyonensis 99.93
    FJAT-49245 Bacillus paramycoides 100.00
    FJAT-49246 Bacillus paramycoides 100.00
    FJAT-49247 假蕈状芽胞杆菌 Bacillus pseudomycoides 99.86
    赖氨酸芽胞杆菌属 Lysinibacillus FJAT-49248 解木糖赖氨酸芽胞杆菌 Lysinibacillus xylanilyticus 99.58
    FJAT-49252 解木糖赖氨酸芽胞杆菌 Lysinibacillus xylanilyticus 99.58
    FJAT-49244 解木糖赖氨酸芽胞杆菌 Lysinibacillus xylanilyticus 99.58
    假单胞菌 Pseudomonas FJAT-49251 扁平假单胞菌 Pseudomonas koreensis 99.57
    FJAT-49249 霍氏假单胞菌 99.43
    Bhargavaea FJAT-49250 Bhargavaea ginsengi 99.72
    山垅非根际土(SLF)
    Irrhizosphere soil in mountain ridge
    芽胞杆菌属 Bacillus FJAT-49253 图瓦永芽胞杆菌 Bacillus toyonensis 99.93
    FJAT-49263 图瓦永芽胞杆菌 Bacillus toyonensis 99.93
    FJAT-49264 图瓦永芽胞杆菌 Bacillus toyonensis 100.00
    FJAT-49262 假蕈状芽胞杆菌 Bacillus pseudomycoides 98.87
    FJAT-49254 假蕈状芽胞杆菌 Bacillus pseudomycoides 100.00
    FJAT-49256 赛门枝芽胞杆菌 Bacillus siamensis 99.65
    FJAT-49257 阿氏芽胞杆菌 Bacillus aryabhattai 100.00
    FJAT-49255 Bacillus albus 100.00
    FJAT-49259 维德曼芽胞杆菌 Bacillus wiedmannii 100.00
    假单胞菌 Pseudomonas FJAT-49258 Pseudomonas granadensis 99.43
    FJAT-49260 Pseudomonas granadensis 99.43
    泛菌属 Pantoea FJAT-49261 Pantoea rodasii 98.72
    山垅非根际土(SLF)
    Rhizosphere soil in mountain ridge
    芽胞杆菌属 Bacillus FJAT-49265 Bacillus proteolyticus 99.93
    FJAT-49266 Bacillus albus 100.00
    FJAT-49269 图瓦永芽胞杆菌 Bacillus toyonensis 99.86
    FJAT-49270 维德曼芽胞杆菌 Bacillus wiedmannii 100.00
    FJAT-49271 高地芽胞杆菌 Bacillus altitudinis 100.00
    FJAT-49273 阿氏芽胞杆菌 Bacillus aryabhattai 100.00
    FJAT-49277 吉氏芽胞杆菌 Bacillus gibsonii 99.86
    FJAT-49280 扁平芽胞杆菌 Bacillus depressus 99.23
    FJAT-49281 扁平芽胞杆菌 Bacillus depressus 99.93
    FJAT-49267 假蕈状芽胞杆菌 Bacillus pseudomycoides 99.93
    假单胞菌 Pseudomonas FJAT-49272 基尔假单胞菌 Pseudomonas koreensis 99.64
    FJAT-49268 基尔假单胞菌 Pseudomonas koreensis 99.64
    FJAT-49275 霍氏假单胞菌 Pseudomonas rhodesiae 99.43
    赖氨酸芽胞杆菌属 Lysinibacillus FJAT-49274 纺锤形赖氨酸芽胞杆菌 Lysinibacillus fusiformis 99.79
    代夫特菌属 Delftia FJAT-49278 食酸丛毛单胞菌 Delftia acidovorans 99.93
    绿芽胞杆菌 Viridibacillus FJAT-49279 田地绿芽胞杆菌 Viridibacillus arvi 100.00
    节杆菌 Arthrobacter FJAT-49276 Arthrobacter bambusae 99.35
    下载: 导出CSV

    表  2  不同种植模式七叶一枝花土壤芽胞杆菌的分离频度(%)和平均数量(×105 cfu·g-1)

    Table  2.   Appearance frequency (%) and average quantity (×105cfu·g-1) of Bacillus spp. isolated from soil specimens

    芽胞杆菌种类
    Bacillus-like species
    SDG SDF SLG SLF LXG LXF 总体 Total
    分离频度 数量 分离频度 数量 分离频度 数量 分离频度 数量 分离频度 数量 分离频度 数量 分离频度 数量
    Bacillus altitudinis 50 18 50 9 50 3 0 0 0 0 0 0 50.00 30
    Bacillus pseudomycoides 50 45 50 16 50 4 50 20 50 7 0 0 83.33 92
    Brevibacterium frigoritolerans 0 0 50 4 0 0 0 0 0 0 0 0 16.67 4
    Bacillus drentensis 50 8 50 3 0 0 0 0 0 0 0 0 33.33 11
    Lysinibacillus mangiferihumi 0 0 50 2 0 0 0 0 0 0 0 0 16.67 2
    Bacillus pacificus 0 0 50 15 0 0 0 0 0 0 0 0 16.67 15
    Bacillus aryabhattai 50 32 50 17 50 10 50 6 0 0 0 0 66.67 65
    Lysinibacillus fusiformis 50 1 50 11 50 4 0 0 0 0 0 0 50.00 16
    Paenarthrobacter ilicis 0 0 50 1 0 0 0 0 0 0 0 0 16.67 1
    Bacillus depressus 50 4 50 4 50 10 0 0 0 0 0 0 50.00 18
    Paenibacillus silvae 0 0 50 3 0 0 0 0 0 0 0 0 16.67 3
    Bacillus toyonensis 50 24 50 7 50 15 50 7 50 92 50 23 100.00 168
    Bacillus timonensis 50 49 0 0 0 0 0 0 0 0 0 0 16.67 49
    Bacillus albus 50 22 0 0 50 4 50 11 0 0 0 0 50.00 37
    Lysinibacillus xylanilyticus 50 13 0 0 0 0 0 0 50 44 50 17 50.00 74
    Bacillus mobilis 50 15 0 0 0 0 0 0 0 0 0 0 16.67 15
    Paenibacillus elgii 50 4 0 0 0 0 0 0 0 0 0 0 16.67 4
    Paenibacillus alvei 50 1 0 0 0 0 0 0 0 0 50 2 33.33 3
    Bacillus cecembensis 50 36 0 0 0 0 0 0 0 0 0 0 16.67 36
    Bacillus proteolyticus 0 0 0 0 50 1 0 0 0 0 50 31 33.33 32
    Bacillus paramycoides 0 0 0 0 0 0 0 0 50 11 50 3 33.33 14
    Delftia lacustris 0 0 0 0 0 0 0 0 0 0 50 1 16.67 1
    Brevibacillus laterosporus 0 0 0 0 0 0 0 0 0 0 50 1 16.67 1
    Burkholderia stabilis 0 0 0 0 0 0 0 0 0 0 50 11 16.67 11
    Pseudomonas rhodesiae 0 0 0 0 50 14 0 0 50 52 0 0 33.33 66
    Bhargavaea ginsengi 0 0 0 0 0 0 0 0 50 29 0 0 16.67 29
    Pseudomonas koreensis 0 0 0 0 50 10 0 0 50 1 0 0 33.33 11
    Bacillus siamensis 0 0 0 0 0 0 50 7 0 0 0 0 16.67 7
    Pseudomonas granadensis 0 0 0 0 0 0 50 12 0 0 0 0 16.67 12
    Bacillus wiedmannii 0 0 0 0 50 3 50 3 0 0 0 0 33.33 6
    Pantoea rodasii 0 0 0 0 0 0 50 8 0 0 0 0 16.67 8
    Arthrobacter bambusae 0 0 0 0 50 150 0 0 0 0 0 0 16.67 150
    Bacillus gibsonii 0 0 0 0 50 1 0 0 0 0 0 0 16.67 1
    Delftia acidovorans 0 0 0 0 50 32 0 0 0 0 0 0 16.67 32
    Viridibacillus arvi 0 0 0 0 50 2 0 0 0 0 0 0 16.67 2
    总计 Tatal - 272 - 92 - 263 - 74 - 236 - 89 - 1026
    注:分离频度 Separation frequency,数量 Amount。SDF:水稻田七叶一枝花非根际 Irrhizosphere soil of Paris polyphylla in paddy field; SDG:水稻田七叶一枝花根际土 Rhizosphere soil of P. polyphylla in paddy field; LXF:林下七叶一枝花非根际土 Irrhizosphere soil of P. polyphylla in understory; LXG:林下七叶一枝花根际土 Rhizosphere soil of P. polyphylla in understory; SLF:山垅七叶一枝花非根际土 Irrhizosphere soil of P. polyphylla in mountain ridge; SLG:山垅七叶一枝花根际土 Rhizosphere soil of P. polyphylla in mountain ridge.
    下载: 导出CSV
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  • 收稿日期:  2019-04-12
  • 修回日期:  2019-07-14
  • 刊出日期:  2019-08-01

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