复合微生物菌对育肥猪生产性能、胃肠道绒毛及菌群结构的影响

李兆龙; 黄宇翔; 罗锦义; 黄耿光; 陈发亮; 黄种杰

doi:10.19303/j.issn.1008-0384.2019.12.007

复合微生物菌对育肥猪生产性能、胃肠道绒毛及菌群结构的影响

doi: 10.19303/j.issn.1008-0384.2019.12.007

1.
福建省农业科学院畜牧兽医研究所，福建　福州　350013
2.
福建省农业科学院作物研究所，福建　福州　350010
3.
南平市农业学校，福建　南平　354200
4.
龙岩市瑞丰养殖有限公司，福建　上杭　364200
5.
泉州华惠生物科技有限公司，福建　泉州　362000

基金项目: 福建省科技计划公益类专项（2018R1101014-7）；龙岩市农业科技重点项目（2019LYF9012)

详细信息

作者简介:
李兆龙（1973−），男，博士，主要从事畜禽肠道微生物及分子育种研究（E-mail：497377512@qq.com）

中图分类号: S 828.5
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出版历程
- 收稿日期: 2019-07-03
- 修回日期: 2019-09-10
- 刊出日期: 2019-12-01

Effects of a Compound Microbial Agent on Weight-gain, Gastrointestinal Villi and Flora of Pigs at Fattening Stage

1.
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350013, China
2.
Crop Research Institute, Fujian Academy of Agricutural Sciences, Fuzhou, Fujian　350010, China
3.
Nanping Agricultural School, Nanping, Fujian　354200, China
4.
Longyan Ruifeng Agriculture Co., Ltd., Shanghang, Fujian　364200, China
5.
Quanzhou Huhui Biotechnology Co., Ltd., Quanzhou, Fujian　362000, China

摘要

摘要: 目的研究复合微生态菌剂对育肥猪猪生产性能、胃肠道绒毛结构及菌群的影响。方法选取300头60日龄25 kg的育肥猪，随机分成对照组和试验组，试验组添加微生态菌剂，对照组添加等量的无菌水。每个处理15个重复，每个重复10头猪。试验为期90 d，记录日采食量、日增重，并测定15、30、60、90 d的耗料、增重比。试验结束时，每处理采集18头猪的胃黏液和结肠黏液，运用Illumina MiSeq方法测定16S rRNA基因并分析，并运用HE染色方法分析育肥猪胃肠道绒毛结构。结果与对照组相比，添加复合微生物菌处理显著提高育肥猪的平均日增重35.53 g（P＜0.05），降低料肉比0.10(P＜0.05)；16S rRNA基因测序结果表明，添加复合微生物菌组的育肥猪胃肠道属水平乳酸杆菌的丰度（平均67.81%）显著高于对照组（13.54%）（P＜0.05)，艰难梭菌属（4.51%）显著低于对照（5.55%）（P＜0.05)。结肠黏液属水平的乳酸菌丰度（平均6.51%）比试验组高（3.17%）（P＜0.05)，结肠黏液属水平艰难梭菌属（2.32%）比对照低（3.73%）（P＜0.05)，艰难梭菌数量显著低于对照组(P＜0.01)。此外，添加复合微生物菌明显增加胃肠道绒毛长度和密度(P＜0.05)。结论复合微生物菌能够显著改善育肥猪的生产性能，增加肠道有益菌，同时改善胃肠道绒毛结构。
- 复合微生物菌 /
- 育肥猪 /
- 生产性能 /
- 16S测序 /
- 绒毛
Abstract: Objective Effects of a compound microecological bioagent on the weight-gain, gastrointestinal villi and micro-flora in pigs at end of growth period were studied． Methods Three-hundred 60-day-old, weighing 25 kg, fattening pigs were randomly divided into control and treatment groups with 15 replicates of 10 pigs in each group. The treatment pigs were fed the forage diet supplemented with a microecological agent, while control with aseptic water. Daily feed-intake and weight-gain of the individual pigs were recorded for 90 d. Ratio of feed consumption/weight-gain was measured on the 15^th, 30^th, 60^th, and 90^th day during the feeding period. At end of the experiment, gastric and colon mucus of 18 randomly selected pigs from each group were collected for the Illumina MiSeq analysis on 16S rRNA gene and the HE-staining on gastrointestinal villi for a microscopic examination. Results The average daily weight-gain of the treatment pigs significantly increased over control (i.e., 35.53 g increase at P＜0.05) with 10 percent reduction on feed-consumption/weight-gain ratio at P＜0.05. The 16S rRNA sequencing showed a significantly higher abundance of Lactobacillus in the gastrointestinal tracts of treatment pigs (averaging 67.81%) than that of control (averaging 13.54%) at P＜0.05, but that of Clostridium difficile (i.e., 4.51%) significantly lower than that of control (i.e., 5.55%) at P＜0.05. In the colon mucus, the genus abundance of Lactobacillus in treatment pigs (i.e., 6.51%) was significantly higher than that in control pigs (i.e., 3.17%) at P＜0.05, but C. difficile (i.e., 2.32%) significantly lower than that of control (i.e., 3.73%) at P＜0.05. The supplementation of the compound microbial agent also significantly increased the length and density of gastrointestinal villi in the treatment pigs (P＜0.05). Conclusion The compound microecological bioagent significantly improved the weight-gain and increased the flora of beneficial bacteria as well as the gastrointestinal villi in the guts on pigs at fattening stage prior to slaughtering.
- compound microecological bioagent /
- fattening pig /
- weight-gain /
- 16S rRNA sequencing /
- villu

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图 1 胃肠道结构及绒毛变化

注：箭头标注为胃肠道绒毛结构；*表示差异显著（P＜0.05），**表示差异极显著(P＜0.01）。　　　　　　　　　　　　　　　　　　

Figure 1. Changes on structure and villi in gastrointestinal tracts

Note：The arrow is marked as the gastrointestinal villi structure; * indicates significant difference (P＜0.05), ** indicates extremenly significant difference (P＜0.01).

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图 2 胃属水平菌群组成

Figure 2. Microbiota composition of gastric mucus with respect to genus

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图 3 结肠属水平菌群组成

Figure 3. Microbiota composition of colon with respect to genus

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表 1 基础日粮组成及营养成分

Table 1. Basic diet and nutritional composition of forage

组成 Composition	含量 Contain
玉米 Corn/%	65
麦皮 Wheat bran/%	3
豆粕 Soybean meal/%	28
预混料 Premix/%（每千克日粮）	4
总计 Total/%	100
消化能 Digestive energy（DE）/（MJ·kg⁻¹）	13.87
粗蛋白 Crude protein（CP）/%	19.5
赖氨酸 Lysine（Lys）/%	1.1
钙 Calcium（Ca）/%	0.9
有效磷 Available phosphorus（AP）/%	0.45
注：预混料组成：石粉40%，磷酸氢钙30%，碘酸钙0.25%，亚硒酸钠0.75%，一水硫酸亚铁1.85%，一水硫酸锌1.5%，五水硫酸铜2.2%，一水硫酸锰0.85%，维生素A0.05%，维生素B1（98%）0.004%，维生素B2（96%）0.02%，维生素B6（98%）0.012%，维生素B12（1%）0.005%，维生素D3 0.09%，维生素E（50%）0.1%，氯化胆碱（50%）3.5%，生物素（2%）0.013%，烟酸（99%）0.076%，叶酸（97%）0.002%，泛酸（98%）0.051%，维生素K3（25%）0.1%，食盐6.0%，蛋氨酸（99%）1.0%，赖氨酸（98%）5.0%，抗氧化剂0.1%，载体（沸石粉）6.558%。 Note：The composition of premix is 40% stone powder, 30% calcium hydrogen phosphate, 0.25% calcium iodate, 0.75% sodium selenite, 1.85% ferrous sulfate monohydrate, 1.5% zinc sulfate monohydrate, 2.2% copper sulfate pentahydrate, 0.85% manganese sulfate monohydrate, 0.05% vitamin A, 0.004% vitamin B1 (98%), 0.02% vitamin B2 (96%), 0.012% vitamin B6 (98%), Vitamin B12 (1%) 0.005%, vitamin D3 0.09%, vitamin E (50%) 0.1%, choline chloride (50%) 3.5%, biotin (2%) 0.013%, niacin (99%) 0.076%, folic acid (97%) 0.002%, pantoic acid (98%) 0.051%, vitamin K3 (25%) 0.1%, salt 6.0%, methionine (99%) 1.0%, lysine (98%) 5.0%, antioxidant 0.1%, carrier (zeolite powder) 6.558%.

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表 2 添加复合菌对育肥猪生产性能的影响

Table 2. Effect of compound microbial agent addition in forage on weight-gain of pigs at fattening stage

试验时间 Time	试验处理 Treatments	平均日采食量 Average daily feed intake（ADFI）/（kg·头⁻¹）	平均增重 Average head gain/（kg·头⁻¹）	平均日增重 Average daily gain（ADG） /（kg·头⁻¹）	料/肉比（F/G） Feed/weight gain	F/G与对照组比较 F/G compared with control
15 d	复合菌组 Treatment	1.60±0.28 a	9.5±0.48 a	0.63±0.15 a	2.53 a	−8.38%
15 d	对照组 Control	1.60±0.39 a	8.72±1.29 b	0.52±0.14 b	2.73 b
30 d	复合菌组 Treatment	1.86±0.98 a	10.01±2.84 a	0.67±0.17a	2.76 a	−2.81%
30 d	对照组 Control	1.88±0.40 a	9.92±1.48 b	0.61±0.10 b	2.84 b
60 d	复合菌组 Treatment	2.43±0.52 a	25.86±1.24 a	0.86±0.15 a	2.82 a	−11.74%
60 d	对照组 Control	1.97±0.19 b	25.08±1.29 b	0.84±0.34 b	3.15 b
90 d	复合菌组 Treatment	3.35±0.76 a	28.65±1.24 a	0.95±0.15 a	3.52 a	−2.63%
90 d	对照组 Control	3.36±0.55 a	27.78±1.29 b	0.93±0.34 b	3.63 b
注：不同小写字母表示同一时间不同处理之间差异显著（P＜0.05），相同字母表示差异不显著（P＞0.05）。 Note: Different letters in the same row means significant difference between treatments（P＜0.05）.

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参考文献(29)

[1]	张兆顺, 窦宝棠, 白应利, 等. 抗生素饲料添加剂在畜牧业生产中过度使用的危害及对策 [J]. 陕西农业科学, 2012, 58(3):127−128, 139. doi: 10.3969/j.issn.0488-5368.2012.03.049 ZHANG Z S, DOU B T, BAI Y L, et al. Harm and countermeasures of excessive use of antibiotic feed additives in animal husbandry production [J]. Shaanxi Journal of Agricultural Sciences, 2012, 58(3): 127−128, 139.(in Chinese) doi: 10.3969/j.issn.0488-5368.2012.03.049
[2]	程学慧, 彭健, 詹志春. 抗生素在畜牧生产中面临的挑战和出路 [J]. 中国畜牧兽医, 2004, 31(2):13−15. doi: 10.3969/j.issn.1671-7236.2004.02.005 CHENG X H, PENG J, ZHAN Z C. The challenge and outlet of antibiotic in animal production [J]. China Animal Husbandry & Veterinary Medicine, 2004, 31(2): 13−15.(in Chinese) doi: 10.3969/j.issn.1671-7236.2004.02.005
[3]	王长彦. 微生物发酵饲料替代饲用抗生素技术在商品猪生产中的应用研究[D]. 杨凌: 西北农林科技大学, 2008. WANG C Y. The application of microbial fermented feed replacing for AGP in the commercial pigs production[D]. Yangling: Northwest A & F University, 2008. (in Chinese)
[4]	CHENG G Y, HAO H H, XIE S Y, et al. Antibiotic alternatives: the substitution of antibiotics in animal husbandry [J]. Frontiers in Microbiology, 2014, 5: 217.
[5]	AARESTRUP F M. Association between the consumption of antimicrobial agents in animal husbandry and the occurrence of resistant bacteria among food animals [J]. International Journal of Antimicrobial Agents, 1999, 12(4): 279−285. doi: 10.1016/S0924-8579(99)90059-6
[6]	ZHAO K N, XU R, ZHANG Y, et al. Development of a novel compound microbial agent for degradation of kitchen waste [J]. Brazilian Journal of Microbiology, 2017, 48(3): 442−450. doi: 10.1016/j.bjm.2016.12.011
[7]	金立明, 生宝强. 饲用微生物添加剂的研究与应用进展 [J]. 北华大学学报(自然科学版), 2004, 5(3):270−272. JIN L M, SHENG B Q. The study and applied progress of microbial addictives in forage [J]. Journal of Beihua University(Natural Science Edition), 2004, 5(3): 270−272.(in Chinese)
[8]	张心如, 杜干英, 张炜. 饲用微生物添加剂在养殖业中的应用(综述) [J]. 养猪, 1996(3):7−11. ZHANG X R, DU G Y, ZHANG W. Application of feed microbial additives in pig industry (Review) [J]. Swine Production, 1996(3): 7−11.(in Chinese)
[9]	欧阳荣林. “EM”菌料饲喂生长育肥猪效果试验 [J]. 畜牧兽医杂志, 2000, 19(3):5−6. OUYANG R L. Experiment on the effect of "EM" bacterial feed on growing and finishing pigs [J]. Journal of Animal Husbandry and Veterinary Medicine, 2000, 19(3): 5−6.(in Chinese)
[10]	王晶, 季海峰, 王四新, 等. 饲用乳酸菌制剂的研究现状及在养猪生产中的应用 [J]. 中国畜牧兽医, 2010, 37(3):38−41. WANG J, JI H F, WANG S X, et al. Advances on Lactobacillus preparation research and application in pig production [J]. China Animal Husbandry & Veterinary Medicine, 2010, 37(3): 38−41.(in Chinese)
[11]	侯成立, 季海峰, 周雨霞, 等. 益生菌的作用机制及其在母猪生产中的应用 [J]. 中国畜牧兽医, 2011, 38(7):20−22. HOU C L, JI H F, ZHOU Y X, et al. Mechanism of probiotics and its application in sow production [J]. China Animal Husbandry & Veterinary Medicine, 2011, 38(7): 20−22.(in Chinese)
[12]	高林, 白子金, 冯波, 等. 微生物饲料添加剂研究与应用进展 [J]. 微生物学杂志, 2014, 34(2):1−6. doi: 10.3969/j.issn.1005-7021.2014.02.001 GAO L, BAI Z J, FENG B, et al. Research and application progress of direct-fed microbials as feed additive [J]. Journal of Microbiology, 2014, 34(2): 1−6.(in Chinese) doi: 10.3969/j.issn.1005-7021.2014.02.001
[13]	徐鹏, 董晓芳, 佟建明. 微生物饲料添加剂的主要功能及其研究进展 [J]. 动物营养学报, 2012, 24(8):1397−1403. doi: 10.3969/j.issn.1006-267x.2012.08.002 XU P, DONG X F, TONG J M. Microbial feed additives: Major functions and its research advances [J]. Chinese Journal of Animal Nutrition, 2012, 24(8): 1397−1403.(in Chinese) doi: 10.3969/j.issn.1006-267x.2012.08.002
[14]	陈秀为. 农业微生物的应用及发展 [J]. 天津农林科技, 1997(1):21−23. CHEN X W. Application and development of agricultural microorganisms [J]. Science and Technology of Tianjin Agriculture and Forestry, 1997(1): 21−23.(in Chinese)
[15]	任瑞兰, 余琏. 猪微生物添加剂饲喂生长育肥猪试验 [J]. 四川农业大学学报, 1994, 12(S1):567−569. REN R L, YU L. Study on growing of piglets microbial additive feed [J]. Journal of Sichuan Agricultural University, 1994, 12(S1): 567−569.(in Chinese)
[16]	马雪花. 微生物饲料的喂猪效果试验 [J]. 浙江畜牧兽医, 2010, 35(4):24. doi: 10.3969/j.issn.1005-7307.2010.04.014 MA X H. Feeding effect test of microbial feed [J]. Zhejiang Journal Animal Science and Veterinary Medicine, 2010, 35(4): 24.(in Chinese) doi: 10.3969/j.issn.1005-7307.2010.04.014
[17]	何志刚, 林小伟. 微生物添加剂对哺乳仔猪增重效果的研究 [J]. 四川农业大学学报, 1994, 12(S1):573−575. HE Z G, LIN X W. Effect of microbial additive in chiet on gain of piglets [J]. Journal of Sichuan Agricultural University, 1994, 12(S1): 573−575.(in Chinese)
[18]	文廷富, 何志刚, 白杋, 等. 猪微生物饲料添加剂对仔猪增重的研究 [J]. 四川农业大学学报, 1994, 12(S1):570−572. WEN T F, HE Z G, BAI F, et al. Study about microbial feed additive on weight gain of suckling pigs [J]. Journal of Sichuan Agricultural University, 1994, 12(S1): 570−572.(in Chinese)
[19]	谢玺文, 张翠霞, 陈丽媛, 等. 饲用微生物的应用及研究现状 [J]. 微生物学杂志, 2001, 21(1):47−49, 53. doi: 10.3969/j.issn.1005-7021.2001.01.017 XIE X W, ZHANG C X, CHEN L Y, et al. Application and research status of feed microorganisms [J]. Journal of Microbiology, 2001, 21(1): 47−49, 53.(in Chinese) doi: 10.3969/j.issn.1005-7021.2001.01.017
[20]	胡未一, 江林, 李东成, 等. 猪微生物饲料添加剂(8701A)对仔猪促生长试验 [J]. 四川农业大学学报, 1994, 12(S1):564−566, 605. HU W Y, JIANG L, LI D C, et al. Effects of microbial feed additive “870lA” on the growth of piglets [J]. Journal of Sichuan Agricultural University, 1994, 12(S1): 564−566, 605.(in Chinese)
[21]	RICHARDS J D, GONG J, DELANGE C F M, 等. 胃肠道菌群及其在单胃动物主要在猪的营养和健康中的作用: 目前的认识, 可能的调节方法及对菌群生态学新的研究方法 [J]. 畜牧与兽医, 2012, 44(S1):357−358. RICHARDS J D, GONG J, DELANGE C F M, et al. Gastrointestinal flora and its role in the nutrition and health of monogastric animals: current understanding, possible regulatory methods and new research methods on flora ecology [J]. Animal Husbandry & Veterinary Medicine, 2012, 44(S1): 357−358.(in Chinese)
[22]	张翥, 董国忠. 影响断奶仔猪小肠结构完整性的因素 [J]. 中国饲料, 2010(6):9−13, 28. doi: 10.3969/j.issn.1004-3314.2010.06.004 ZHANG Z, DONG G Z. Factors affecting the structural integrality of the small intestine in weanling pigs [J]. China Feed, 2010(6): 9−13, 28.(in Chinese) doi: 10.3969/j.issn.1004-3314.2010.06.004
[23]	李长军, 张玉仙, 华勇谋, 等. 微生态制剂对新生仔猪小肠绒毛形态结构和黏膜免疫相关细胞的影响 [J]. 黑龙江畜牧兽医, 2011(19):60−62. LI C J, ZHANG Y X, HUA Y M, et al. Effect of Microecological Preparation on the Morphological Structure and Mucosal Immunorelated Cells of Newborn Piglets [J]. Heilongjiang Animal Science and Veterinary Medicine, 2011(19): 60−62.(in Chinese)
[24]	孙媛. 液态复合微生态制剂的研制及在仔猪生产上的应用[D]. 长春: 吉林农业大学, 2006. SUN Y. The liquid compound mcirobial eclolgical agents to research and manufacture and in application of the weaning piglets farming[D]. Changchun: Jilin Agricultural University, 2006. (in Chinese)
[25]	欧阳志周, 朱文, 肖林, 等. 猪源丁酸梭菌分离及其对肠道绒毛发育的影响 [J]. 齐鲁工业大学学报(自然科学版), 2016, 30(3):28−31. OUYANG Z Z, ZHU W, XIAO L, et al. Influence of the isolated Clostridium butyricum strain on the growth of intestinal villi [J]. Journal of Qilu University of Technology(Natural Science Edition), 2016, 30(3): 28−31.(in Chinese)
[26]	华建平, 姚宏昌, 马桂凤. 功能性消化不良患者的胃动力学研究 [J]. 天津医药, 2000, 28(3):172−174. doi: 10.3969/j.issn.0253-9896.2000.03.015 HUA J P, YAO H C, MA G F. The study of gastric dynamics in patients with functional dyspepsia [J]. Tianjing Medical Journal, 2000, 28(3): 172−174.(in Chinese) doi: 10.3969/j.issn.0253-9896.2000.03.015
[27]	WANG Y, YI L, ZHAO M L, et al. Effects of zinc-methionine on growth performance, intestinal flora and immune function in pigeon squabs [J]. British Poultry Science, 2014, 55(3): 403−408. doi: 10.1080/00071668.2014.919375
[28]	杨红萍, 鲍玉林. 复合微生态制剂对仔猪生长性能、肠道微生物和免疫功能的影响[ [J]. 黑龙江畜牧兽医, 2017, 522(6):74−76. YANG H P, BAO Y L. Effect of compound microecological preparation on growth performance, intestinal microorganism and immune function of piglets [J]. Heilongjiang Animal Husbandry and Veterinary, 2017, 522(6): 74−76.(in Chinese)
[29]	杜泓明. 不同益生菌培养物对断奶仔猪生长性能、免疫功能及盲肠微生物区系的影响[D]. 长春: 吉林农业大学, 2017. DU H M. Effect of different probiotic cultures on growth performance, immune function and cecum microflora diversity of weaned piglets[D]. Changchun: Jilin Agricultural University, 2017. (in Chinese)