培养基和植物生长调节剂对油㮈种胚培育效应

陈洁; 刘瑾航; 李永裕; 吴少华; 廖汝玉

doi:10.19303/j.issn.1008-0384.2020.03.009

培养基和植物生长调节剂对油㮈种胚培育效应

doi: 10.19303/j.issn.1008-0384.2020.03.009

陈洁^{1, 2,},
刘瑾航²,
李永裕²,
吴少华^2, ,,
廖汝玉^1, ,

1.
福建省农业科学院果树研究所，福建　福州　350013
2.
福建农林大学园艺学院，福建　福州　350002

基金项目: 福建省科技计划公益类专项（2019R1028-8）；国家科技支撑计划项目（2013BAD02B03-4-1）

详细信息

作者简介:
陈洁（1996−），女，硕士研究生，主要从事油㮈胚培育研究（E-mail: 1312680417@qq.com）

通讯作者:
吴少华（1959−），男，硕士，教授，博士生导师，主要从事园艺植物遗传育种、园艺活性物质提纯和利用、园艺疗法与景观规划（E-mail: 348279953@qq.com）

廖汝玉（1974−），男，硕士，副研究员，主要从事落叶果树育种与栽培研究（E-mail: 416944528@qq.com）

中图分类号: S 662.3
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出版历程
- 收稿日期: 2019-11-12
- 修回日期: 2020-01-21
- 刊出日期: 2020-03-01

Effects of Medium and Plant Growth Regulator on Germination of Prunus salicina Embryos

CHEN Jie^{1, 2
,},
LIU Jin-hang²,
LI Yong-yu²,
WU Shao-hua^{2
, ,},
LIAO Ru-yu^{1
, ,}

1.
College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China
2.
Institute of Fruit Research, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350013, China

摘要

摘要: 目的筛选适宜不同状态油㮈种胚的胚培育条件，提高种胚萌发率。方法以福建特色果树油㮈为试验材料，分析油㮈种胚状态、培养基种类以及植物生长调节剂对油㮈种胚萌发的影响，筛选出合适的培养基及植物生长调节剂组合进行胚培育，提高种胚的萌发率。结果在不添加植物生长调节剂的条件下，接种于WPM培养基的半败育种胚最高的萌发率为40.00%；在添加植物生长调节剂的条件下，接种于WPM培养基添加2.0 mg·L⁻¹ 6-BA+0.1 mg·L⁻¹ IBA（6号）的半败育种胚都有着最高的萌发率，为46.67%。完整种胚的最高萌发率为100.00%，分别为不添加任何植物生长调节剂的WPM培养基、MS培养基或添加1.0 mg·L⁻¹ 6-BA+0.1 mg·L⁻¹ NAA（2号）、2.0 mg·L⁻¹ 6-BA+0.2 mg·L⁻¹ NAA（3号）、2.0 mg·L⁻¹ 6-BA+0.1 mg·L⁻¹ IBA（6号）的WPM培养基。结论最适宜油㮈种胚萌发的是WPM培养基+2.0 mg·L⁻¹ 6-BA+0.1 mg·L⁻¹ IBA，且完整胚的萌发率都要高于半败育的萌发率。不添加植物生长调节剂的WPM培养基和MS培养基也都具有较好的萌发效果。
- 油㮈 /
- 种胚 /
- 植物生长调节剂 /
- 培养基 /
- 离体培养
Abstract: Objective Optimal medium formulation and effect of plant growth regulators for germination of Prunus salicina var. cordata cv. Younai embryos were investigated. Method Culture media with or without addition of plant growth regulators were varied to determine the effects on the embryonic germination of Younai seeds. Result The highest germination rate of the semi-abortive breeding embryos inoculated in the WPM medium without any added growth regulator was 40.00%. With the regulator addition, the WPM medium including 2.0 mg·L⁻¹ 6-BA and 0.1 mg·L⁻¹ IBA (Treatment No. 6) achieved a maximum germination rate of 46.67%. For the intact embryos, the rate reached 100.00% on either MS, WPM, or WPM with the addition of either 1.0 mg·L⁻¹ 6-BA and 0.1 mg·L⁻¹ NAA (Treatment No. 2) or 2.0 mg·L⁻¹ 6-BA and 0.2 mg·L⁻¹ NAA (Treatment No. 3) or Treatment No. 6. Conclusion The WPM medium with 2.0 mg·L⁻¹ 6-BA and 0.1 mg·L⁻¹ IBA delivered the highest germination rate of the semi-abortive seed embryos among all treatments. The germination of the intact embryos was found always higher than that of semi-abortive embryos. And, without added plant growth regulators, the WPM or MS medium could, nonetheless, provide acceptable embryo germination for Younai seeds.
- Prunus salicina var. cordata cv. Younai /
- embryo /
- plant growth regulator /
- medium /
- in vitro culture

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表 1 3种植物生长调节剂（6-BA、IBA、NAA）的正交组合

Table 1. Orthogonal combinations of 3 plant growth regulators, 6-BA, IBA and NAA

植物生长调节剂编号 Plant growth regulator number	6-BA/（mg·L⁻¹)	IBA/（mg·L⁻¹)	NAA/（mg·L⁻¹)
1（CK）	0	0	0
2	1.0	0	0.1
3	2.0	0	0.2
4	0	0.1	0.1
5	1.0	0.1	0.2
6	2.0	0.1	0
7	0	0.2	0.2
8	1.0	0.2	0
9	2.0	0.2	0.1

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表 2 不同类型种胚在3种培养基上的萌发情况

Table 2. Germinations of two types of embryos on 3 media

培养基种类 Culture medium kind	半败育胚萌发率/%	完整胚萌发率 /%	总萌发率/%
A（SH培养基）	0.90	18.89	6.11
B（WPM培养基）	17.11	64.00	25.54
C（MS培养基）	1.50	68.54	18.31
注：半败育胚萌发率（萌发个数/半败育胚个数）；完整胚萌发率（萌发个数/完整胚个数）；总萌发率（总萌发数/总胚个数）。 Note: Germination rate of semi-abortive embryos (number of germinated embryos/number of semi-aborted embryos); germination rate of intact complete embryos (number of germinated intact embryos/number of intact embryos); total germination rate (total number of germinated embryos/total number of embryos).

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表 3 培育于SH培养基中油㮈种胚的萌发率

Table 3. Germination rate of seed embryos cultured in SH medium

植物生长调节剂组合类型 Combination types of plant growth regulators	半败育种胚萌发率 Germination rate of semi-abortive breeding embryo/%	完整种胚萌发率 Germination rate of intact embryo/%
1（CK）	0	0
2	0	7.14±7.15 de
3	3.33±5.77	0
4	0	30.95±4.12 c
5	0	14.29±7.15 d
6	0	61.90±8.25 a
7	0	9.52±4.13 de
8	0	50.00±14.29 b
9	0	9.52±4.13 de
注：同一列不同小写字母表示差异显著（P＜0.05）。表4～6同。 Note: Data with different lowercase letters on same column indicate significant difference（P＜0.05）. The same as table 4−6.

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表 4 培育于WPM培养基中油㮈种胚的萌发率

Table 4. Germination rate of seed embryos cultured in WPM medium

植物生长调节剂组合类型 Combination types of plant growth regulators	半败育种胚萌发率 Germination rate of semi-abortive breeding embryo/%	完整种胚萌发率 Germination rate of intact embryo/%
1（CK）	40.00±0.00 b	100.00±0.00 a
2	0	100.00±0.00 a
3	16.67±5.77 d	100.00±0.00 a
4	23.33±5.77 c	0
5	10.00±0.00 e	50.00±0.00 d
6	46.67±5.77 a	100.00±0.00 a
7	0	76.19±4.12 b
8	0	61.91±4.13 c
9	10.00±0.00 e	11.91±4.13 e

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表 5 培育于MS培养基中油㮈种胚的萌发率

Table 5. Germination rate of seed embryos cultured in MS medium

植物生长调节剂组合类型 Combination types of plant growth regulators	半败育种胚萌发率 Germination rate of semi-abortive breeding embryo/%	完整种胚萌发率 Germination rate of intact embryo/%
1（CK）	0	90.48±4.13 a
2	0	0
3	3.33±5.77 ab	0
4	0	90.48±10.91 a
5	0	50.00±7.14 c
6	0	71.43±7.14 b
7	6.67±5.77 a	57.14±7.15 c
8	0	57.14±7.15 c
9	3.33±5.77 ab	80.95±4.12 ab

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表 6 不同组合植物生长调节剂的萌发情况

Table 6. Germination of seed embryos on media with varied combinations of plant growth regulators

植物生长调节剂组合类型 Combination types of plant growth regulators	半败育种胚萌发率 Germination rate of semi-abortive breeding embryo/%	完整种胚萌发率 Germination rate of intact embryo/%
1（CK）	13.33±0.00 a	63.49±1.38 b
2	2.22±1.92 c	35.71±2.39 fg
3	7.78±1.92 b	33.33±0.00 g
4	7.78±1.92 b	40.48±2.38 e
5	3.33±0.00 c	38.10±2.39 ef
6	15.56±1.93 a	77.78±3.64 a
7	2.22±1.92 c	47.62±0.00 d
8	2.22±1.92 c	56.35±2.75 c
9	4.44±1.93 c	34.12±1.37 g

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