不同施氮水平对核桃砧木苗形态特征及生理特性的影响

宋岩; 张锐; 鱼尚奇; 刘春花; 高山; 王雨; 罗立新; 张建良

doi:10.19303/j.issn.1008-0384.2020.03.010

不同施氮水平对核桃砧木苗形态特征及生理特性的影响

doi: 10.19303/j.issn.1008-0384.2020.03.010

宋岩^{1, 2, 3,},
张锐^{1, 2, 3, ,},
鱼尚奇^{1, 4},
刘春花^{1, 2, 3},
高山²,
王雨^{2, 3},
罗立新⁵,
张建良⁵

1.
新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室新疆　阿拉尔　843300
2.
塔里木大学植物科学学院新疆　阿拉尔　843300
3.
南疆特色果树高效优质栽培与深加工技术国家地方联合工程实验室新疆　阿拉尔　843300
4.
塔里木大学生命科学学院新疆　阿拉尔　843300
5.
新疆生产建设兵团第一师三团林业站新疆　阿拉尔　843300

基金项目: 兵团师域（区域）发展创新支持计划项目（2017DB001）；第一师阿拉尔市科研课题计划项目（2017YY20）；研究生科研创新项目（TDGRI201820）

详细信息

作者简介:
宋岩（1993−），男，硕士研究生，研究方向：果树遗传育种（E-mail: cliff_sy@163.com）

通讯作者:
张锐（1979−），教授，博士生导师，研究方向：果树遗传育种（E-mail: zhrgsh@163.com）

中图分类号: S 664.1
计量
- 文章访问数: 776
- HTML全文浏览量: 268
- PDF下载量: 16
- 被引次数: 0
出版历程
- 收稿日期: 2019-11-24
- 修回日期: 2020-01-05
- 刊出日期: 2020-03-01

Morphology and Physiology of Walnut Stock Seedlings as Affected by Nitrogen Fertilizations

SONG Yan^{1, 2, 3
,},
ZHANG Rui^{1, 2, 3
, ,},
YU Shang-qi^{1, 4},
LIU Chun-hua^{1, 2, 3},
GAO Shan²,
WANG Yu^{2, 3},
LUO Li-xin⁵,
ZHANG Jian-liang⁵

1.
Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basi¹, Xinjiang Production and Construction Corps, Alar, Xinjiang　843300, China
2.
College of Plant Science and Technology, Tarim University, Alar, Xinjiang　843300, China
3.
National and Local Joint Engineering Laboratory of High Efficiency and Superior-Quality Cultivation and Fruit Deep Processing Technology of Characteristic Fruit Trees in South, Alar, Xinjiang　843300, China
4.
College of Life Science, Tarim University, Alar, Xinjiang　843300, China
5.
First Division of the Third Group Forestry Station, Xinjiang Production and Construction Corps, Alar, Xinjiang　843300, China

摘要

摘要: 目的探究不同氮水平对2个种源地的核桃砧木苗形态特征、生理特性的影响，为新疆核桃砧木苗在栽培过程中的标准化施肥提供理论基础。方法对阿克苏种源地及和田种源地的核桃砧木苗进行不同水平的氮素诱导，测定核桃砧木苗地上部及地下部形态特征、生物量、光合特征及叶片可溶性糖含量。结果相同氮素水平处理下的不同种源地核桃砧木苗部分指标存在差异且各指标随氮梯度递增表现出先扬后抑的趋势。当施肥量达到10−15 g·a⁻¹·株⁻¹时，株高、根系总体积达到最高值，株高：95.27 、72.07 cm，根系体积：1 249.75 、928.01 cm³；净光合速率随着氮水平的增高而增高，施肥量达到10 g·a⁻¹·株⁻¹时出现最大值12.725 1、12.357 3 μmol·m⁻²·s⁻¹，之后随氮水平的递增，净光合速率逐渐下降。综合评价得出施氮量在10−15 g·a⁻¹·株⁻¹的2个种源地的核桃砧木苗形态特征及生理特性均表现出最优性状。结论核桃砧木苗的标准化施氮范围为10−15 g·a⁻¹·株⁻¹，且在标准化施肥范围内，阿克苏种源地核桃砧木苗对氮素的利用能力强于和田种源地的核桃砧木苗。
- 氮水平 /
- 核桃砧木 /
- 种源地 /
- 形态特征 /
- 光合作用
Abstract: Objective Varied N levels for fertilization were applied to determine the effects on the morphological and physiological characteristics of the Juglans regia L. rootstock seedlings from two provenances in Xinjiang. Methods Juglans regia L. rootstock seedlings from provenances at Aksu and Hotan were treated with varied levels of N fertilizer, and the morphology, biomass, photosynthetic characteristics, and soluble sugar content in leaves of the seedlings determined. Results The various evaluation indicators tended to increase initially and followed by a decline upon increasing N but responded differently between the seedling specimens from the two localities. Under 10−15 g·a⁻¹·plant⁻¹ application rates, the plant height peaked at 95.27 cm and 72.07 cm, while the total root volume at 1 249.75 cm³ and 928.01 cm³, respectively, for the Juglans regia L. plants from the two sources. The net photosynthetic rates for them increased with N to arrive at a maximum of 12.725 1 μmol·m⁻²·s⁻¹ and 12.357 3 μmol·m⁻²·s⁻¹, respectively, under the 10 g·a⁻¹·plant⁻¹ fertilization. Further increases on N caused a gradual decline on the net photosynthetic rate. According to the comprehensive evaluation, the morphological characteristics and physiological characteristics of Juglans regia L. rootstock seedlings in the two provenances with nitrogen application at 10−15 g·a⁻¹·plant⁻¹ showed the optimal characters. Conclusion Over all, the Juglans regia L. rootstock seedlings from the two provenances performed well morphologically and physiologically under the applications of 10−15 g·a⁻¹·plant⁻¹. In that range, Aksu walnut exhibited a greater N utilization ability than its counterpart.
- nitrogen level /
- rootstock seedling /
- provenance /
- morphology /
- photosynthesis

HTML全文

图 1 阿克苏种源地核桃砧木苗根系形态对氮水平的响应

Figure 1. Morphology of Aksu walnut rootstocks in response to varied N level

下载: 全尺寸图片幻灯片

图 2 和田种源地核桃砧木苗根系形态对氮水平的响应

Figure 2. Morphology of Hotan walnut rootstocks in response to varied N level

下载: 全尺寸图片幻灯片

图 3 不同处理间核桃砧木苗生物量对氮水平的响应

Figure 3. Biomass of walnut stock seedlings in response to varied treatment

下载: 全尺寸图片幻灯片

图 4 不同处理间核桃砧木苗叶片可溶性糖含量对氮水平的响应

Figure 4. Soluble sugar content of walnut stock seedlings in response to varied treatment

下载: 全尺寸图片幻灯片

表 1 不同处理间核桃砧木苗根系形态特征对氮水平的响应

Table 1. Morphological characteristics of seedling roots in response to varied treatment

种源地 Provenance	处理 Treatment	总长度 Total length/ cm	总表面积 Total surface area/ cm²	总体积 Total volume/ cm³	平均直径 Average diameter/ mm	总根尖数 Total root tip amount/ （N·plant⁻¹）
阿克苏种源地（A）	N1（CK）	2 060.29±34.40 c	1 593.14±17.25 d	668.34±12.46 d	1.14±0.043 2 d	9 469.33±311.71 c
	N2	3 045.11±37.12 b	1 859.40±27.35 b	763.63±29.15 c	1.29±0.007 8 c	9 470.33±132.00 c
	N3	3 362.79±22.76 a	2 033.67±10.28 a	977.73±9.94 b	1.43±0.004 7 b	16 310.67±520.96 a
	N4	2 191.47±95.27 c	1 758.70±9.08 c	1 249.75±20.63 a	1.50±0.021 7 a	12 249.33±204.33 b
	N5	1 698.38±40.65 d	1 430.50±9.39 e	586.01±2.90 e	1.14±0.013 7 d	7 297.00±175.65 d
和田种源地（H）	N1（CK）	2 013.64±2.53 c	1 436.31±15.18 d	445.53±19.62 d	0.89±0.003 7 d	11 671.33±177.75 b
	N2	2 447.27±81.84 b	1 739.95±27.63 c	670.18±8.61 c	1.15±0.012 2 c	11 791.00±288.70 b
	N3	2 941.66±20.64 a	1 957.46±27.79 a	928.01±4.60 a	1.59±0.016 3 a	14 558.00±267.22 a
	N4	1 413.87±4.13 d	1 875.53±8.76 b	868.13±16.30 b	1.55±0.022 3 b	7 862.00±601.13 c
	N5	945.41 ±3.01 e	1 136.71±19.73 e	422.70±5.20 d	1.17±0.013 4 c	4 550.33±294.24 d
注：同一列数字后不同小写字母表示不同处理间差异达到5%显著水平，表2～4同。 Note: Data with different lowercase letters after same column indicate significant difference between different treatments at 5% level. Same for the following. The same as table 2-4.

下载: 导出CSV

表 2 不同处理间核桃砧木苗形态特征对氮水平的响应

Table 2. Morphological characteristics of walnut stock seedlings in response to varied treatment

种源地 Provenance	处理 Treatment	叶长 Leaf length/ mm	叶宽 Leaf width/ mm	叶厚 Leaf thickness/ mm	叶片数 Number of leaf/ （piece·plant⁻¹）	株高 Plant height/ cm	地径 Ground diameter/ mm
阿克苏种源地（A）	N1（CK）	102.49±2.79 d	59.78±3.49 b	0.27±0.010 0 b	100.67±3.48 d	65.23±1.83 c	13.32±1.28 b
	N2	124.73±1.72 bc	72.00±1.53 a	0.34±0.008 8 a	122.00±5.03 c	78.90±5.12 b	14.61±0.15 b
	N3	144.89±8.52 a	77.48±0.97 a	0.32±0.016 7 a	149.00±1.53 b	81.13±7.38 b	16.93±0.49 a
	N4	134.00±15.06 ab	76.78±1.66 a	0.33±0.017 6 a	170.33±5.36 a	95.27±1.53 a	18.22±0.62 a
	N5	116.00±3.71 cd	73.92±1.90 a	0.30±0.003 3 ab	141.00±3.00 b	83.13±2.23 ab	12.83±0.29 b
和田种源地（H）	N1（CK）	108.98±0.88 b	64.20±3.21 bc	0.39±0.006 7 d	110.00±1.00 c	62.83±0.26 b	12.81±0.05 d
	N2	127.89±3.13 b	67.22±0.67 abc	0.47±0.003 3 c	130.33±3.28 b	69.03±3.93 ab	14.99±0.34 b
	N3	165.11±12.95 a	70.99±1.09 a	0.52±0.020 3 b	154.33±2.19 a	72.07±0.52 a	17.19±0.07 a
	N4	123.75±1.59 b	62.84±0.14 c	0.59±0.006 7 a	134.33±4.84 b	63.77±0.74 ab	16.77±0.21 a
	N5	126.89±1.25 b	68.33±1.68 ab	0.37±0.015 3 d	130.67±2.40 b	67.50±4.32 ab	14.07±0.49 c

下载: 导出CSV

表 3 不同处理间核桃砧木苗光合参数对氮水平的响应

Table 3. Photosynthetic parameters on walnut stock seedlings in response to varied treatment

种源地 Provenance	处理 Treatment	Pn/（μmol·m⁻²·s⁻¹）	Ci/（μmol·mol⁻¹）	Gs/（mol·m⁻²·s⁻¹）	Tr/（mmol·m⁻²·s⁻¹）
阿克苏种源地（A）	N1（CK）	9.36±0.12 c	243.49±8.99 a	0.141±0.004 a	5.59±0.09 c
	N2	10.56±0.55 bc	215.21±5.79 b	0.145±0.002 a	5.75±0.02 bc
	N3	12.73±0.50 a	211.43±4.86 b	0.157±0.006 a	6.60±0.19 a
	N4	11.59±0.71 ab	187.59±7.43 c	0.149±0.001 a	6.48±0.11 a
	N5	9.49±0.75 c	206.89±5.17 bc	0.149±0.010 a	5.96±0.03 b
和田种源地（H）	N1（CK）	12.00±0.40 ab	248.17±2.52 a	0.150±0.007 b	6.774±0.49 ab
	N2	11.79±0.79 ab	230.00±7.01 b	0.154±0.002 b	7.59±0.46 a
	N3	12.36±0.07 a	196.35±5.27 d	0.177±0.003 a	7.07±0.54 ab
	N4	12.06±0.86 ab	208.03±3.75 cd	0.170±0.008 ab	7.27±0.21 ab
	N5	10.56±0.14 b	223.27±5.96 bc	0.161±0.009 ab	6.32±0.09 b

下载: 导出CSV

表 4 不同处理间核桃砧木苗光合色素对氮水平的响应

Table 4. Photosynthetic pigments of walnut stock seedlings in response to varied treatment

种源地 Provenance	处理 Treatment	Chl a/（mg·g⁻¹）	Chl b/（mg·g⁻¹）	Car/（mg·g⁻¹）	Chl a+b/（mg·g⁻¹）
阿克苏种源地（A）	N1（CK）	1.31±0.13 a	0.60±0.04 b	0.83±0.11 a	1.91±0.16 a
	N2	1.96±0.19 a	0.68±0.03 ab	0.92±0.07 a	2.64±0.21 a
	N3	2.69±0.19 a	0.92±0.09 a	0.97±0.06 a	3.61±0.27 a
	N4	2.05±0.28 a	0.85±0.03 ab	0.81±0.08 a	2.90±0.31 a
	N5	2.12±0.07 a	0.73±0.08 ab	0.82±0.08 a	2.86±0.15 a
和田种源地（H）	N1（CK）	1.51±0.15 c	0.59±0.08 c	0.70±0.03 bc	2.10±0.23 c
	N2	1.58±0.31 b	0.70±0.09 bc	0.73±0.02 ab	2.28±0.37 b
	N3	1.70±0.03 a	0.79±0.06 a	0.85±0.02 a	2.49±0.09 a
	N4	1.69±0.08 b	0.73±0.08 ab	0.73±0.05 c	2.42±0.16 b
	N5	1.54±0.09 b	0.66±0.02 abc	0.66±0.01 c	2.20±0.03 b

下载: 导出CSV

参考文献(31)

[1]	梁娟, 胡海丽, 杨伟. 不同供氮水平对天门冬生长和品质的影响 [J]. 中国土壤与肥料, 2016(1):53−56. doi: 10.11838/sfsc.20160109 LIANG J, HU H L, YANG W. Effects of different nitrogen levels on growth and quality of Asparagus Cochinchinensis(Lour.) Merr [J]. Soil and Fertilizer Sciences in China, 2016(1): 53−56.(in Chinese) doi: 10.11838/sfsc.20160109
[2]	赵庆杰, 柴露, 吴金山. 海滨雀稗对氮素施用水平的生长及生理响应 [J]. 江苏农业科学, 2018, 46(9):153−155. ZHAO Q J, CHAI LU, WU J S. Response of growth and physiology of Paspalum vaginatum to nitrogen application levels [J]. Jiangsu Agricultural Sciences, 2018, 46(9): 153−155.(in Chinese)
[3]	匡鹤凌, 汪贵斌, 曹福亮. 不同供氮水平对喜树造林幼树生长的影响 [J]. 林业科技开发, 2015, 29(6):66−69. KUANG H L, WANG G B, CAO F L. Effects of nitrogen application on the growth of Camptothecaacuminata seedlings [J]. China Forestry Science and Technology, 2015, 29(6): 66−69.(in Chinese)
[4]	王益明, 万福绪, 李瑞瑞, 等. 指数施肥对美国山核桃幼苗生长及养分积累的影响 [J]. 东北林业大学学报, 2018, 46(9):21−25. doi: 10.3969/j.issn.1000-5382.2018.09.005 WANG Y M, WAN F X, LI R R, et al. Effects of exponential fertilization on growth and nutrient accumulation of Carya illinoensis seedlings [J]. Journal of Northeast Forestry University, 2018, 46(9): 21−25.(in Chinese) doi: 10.3969/j.issn.1000-5382.2018.09.005
[5]	马亚东, 刘慧英, 张筱茜, 等. 不同供氮水平对苦苣干物质积累、光合特性及叶绿素荧光参数的影响 [J]. 石河子大学学报(自然科学版), 2016, 34(3):315−320. MA Y D, LIU H Y, ZHANG X Q, et al. Effect of different nitrogen levels on dry matter accumulation, photosynthetic characteristics and chlorophyll fluorescence of endive(Cichorium endivia L.) [J]. Journal of Shihezi University(Natural Science), 2016, 34(3): 315−320.(in Chinese)
[6]	许楠, 倪红伟, 钟海秀, 等. 不同供氮水平对饲料桑树幼苗生长以及光合特性的影响 [J]. 江苏农业学报, 2015, 31(4):865−870. doi: 10.3969/j.issn.1000-4440.2015.04.024 XU N, NI H W, ZHONG H X, et al. Growth and photosynthetic characteristics of forage mulberry in response to different nitrogen application levels [J]. Jiangsu Journal of Agricultural Sciences, 2015, 31(4): 865−870.(in Chinese) doi: 10.3969/j.issn.1000-4440.2015.04.024
[7]	刘莹, 史树德. 不同施氮水平下甜菜光合特性比较 [J]. 北方农业学报, 2016, 44(2):7−12. doi: 10.3969/j.issn.1007-0907.2016.02.002 LIU Y, SHI S D. The photosynthetic characters of sugar beet under different nitrogen levels [J]. Journal of Northern Agriculture, 2016, 44(2): 7−12.(in Chinese) doi: 10.3969/j.issn.1007-0907.2016.02.002
[8]	沙建川, 贾志航, 徐新翔, 等. 氮水平对苹果叶片¹³C光合产物和¹⁵N向果实转移分配的影响 [J]. 应用生态学报, 2019, 30(4):1373−1379. SHA J C, JIA Z H, XU X X, et al. Effects of nitrogen application levels on translocation and distribution of ¹³C-photosynthate and ¹⁵N to fruit from leaves of apple tree [J]. Chinese Journal of Applied Ecology, 2019, 30(4): 1373−1379.(in Chinese)
[9]	丁易飞, 申长卫, 王洁, 等. 不同施氮水平对棚架栽培‘寿新水’梨生长及山梨醇代谢的影响 [J]. 南京农业大学学报, 2017, 40(2):242−250. doi: 10.7685/jnau.201604055 DING Y F, SHEN C W, WANG J, et al. Effects of different supply of N levels on tree growth and sorbitol metabolism in leaf and fruit of trellis trained ‘Kotobuki shinsui’ pear [J]. Journal of Nanjing Agricultural University, 2017, 40(2): 242−250.(in Chinese) doi: 10.7685/jnau.201604055
[10]	李国和. 核桃种质资源研究[D]. 雅安: 四川农业大学, 2007. LI G H. Studies on germplasm resources of walnuts[D]. Yaan, China: Sichuan Agricultural University, 2007. (in Chinese)
[11]	李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000.
[12]	樊卫国, 罗燕. 不同磷水平下4种柑橘砧木的生长状况、根系形态和生理特性 [J]. 中国农业科学, 2015, 48(3):534−545. doi: 10.3864/j.issn.0578-1752.2015.03.13 FAN W G, LUO Y. Growth status, root morphology and physiological characteristics of four Citrus rootstocks under different phosphorus levels [J]. Scientia Agricultura Sinica, 2015, 48(3): 534−545.(in Chinese) doi: 10.3864/j.issn.0578-1752.2015.03.13
[13]	王祎, 汤继华, 付延磊, 等. 不同氮水平下玉米苗期根系形态和氮吸收量的QTL定位 [J]. 植物营养与肥料学报, 2017, 23(4):942−956. doi: 10.11674/zwyf.16461 WANG Y, TANG J H, FU Y L, et al. Mapping of QTLs for root morphology and nitrogen uptake of maize under different nitrogen conditions [J]. Journal of Plant Nutrition and Fertilizer, 2017, 23(4): 942−956.(in Chinese) doi: 10.11674/zwyf.16461
[14]	Merghany M M. Effect of irrigation system and nitrogen levels on vegetative growth, yield, yield components and some chemical composition of tomato plants growth in newly reclaimed sandy soils [J]. Annals of Agricultural Science, 1997, 35(2): 965−981.
[15]	段英华, 张亚丽, 沈其荣. 水稻根际的硝化作用与水稻的硝态氮营养 [J]. 土壤学报, 2004, 41(5):803−809. doi: 10.3321/j.issn:0564-3929.2004.05.021 DUAN Y H, ZHANG Y L, SHEN Q R. Nitrification in rice rhizosphere and the nitrate nutrition of rice [J]. Acta Pedologica Sinica, 2004, 41(5): 803−809.(in Chinese) doi: 10.3321/j.issn:0564-3929.2004.05.021
[16]	施侃侃, 梁栋, 贾忠奎. 不同氮磷钾配比对红花玉兰幼苗生长及养分转运的影响 [J]. 中南林业科技大学学报, 2018, 38(9):58−64. SHI K K, LIANG D, JIA Z K. Effects of different ratio of N, P, K on nutrient accumulation and translocation of Magnolia wufengensis [J]. Journal of Central South Forestry University, 2018, 38(9): 58−64.(in Chinese)
[17]	刘根华, 黄坚钦, 潘春霞, 等. 基于反射光谱的山核桃幼苗氮素营养状况分析 [J]. 林业科学, 2011, 47(1):165−171. doi: 10.11707/j.1001-7488.20110125 LIU G H, HUANG J Q, PAN C X, et al. Analysis of the nitrogen levels in leaves of hickory seedling by reflectance spectra [J]. Scientia Silvae Sinicae, 2011, 47(1): 165−171.(in Chinese) doi: 10.11707/j.1001-7488.20110125
[18]	康晓育, 孙协平, 常聪, 等. 氮素形态对不同苹果砧木幼苗生长的影响 [J]. 西北农林科技大学学报(自然科学版), 2013, 41(6):133−138. KANG X Y, SUN X P, CHANG C, et al. Effects of nitrogen forms on growth of different Malus rootstock seedlings [J]. Journal of Northwest A & F University(Natural Science Edition), 2013, 41(6): 133−138.(in Chinese)
[19]	张亚洁, 苏祖芳, 杨连新, 等. 施氮水平对旱秧本田期氮素营养和根系生长及产量形成的影响 [J]. 扬州大学学报(农业与生命科学版), 2001(1):21−26. ZHANG Y J, SU Z F, YANG X L, et al. Effect of nitrogen supplying level on nitrgogen nutrition and the growth of roots and yield formation of dry nursery seedlings of rice in field [J]. Journal of Yangzhou University(Agricultural and Life Science Edition), 2001(1): 21−26.(in Chinese)
[20]	杨阳, 施皓然, 及利, 等. 指数施肥对紫椴播种苗生长和根系形态的影响[J/OL]. 南京林业大学学报(自然科学版): 1-8[2019-06-30]. http://kns.cnki.net/kcms/detail/32.1161.S.20190319.1728.008.html. YANG Y, SHI H R, JI L, et al. Effects of exponential fertilization on growth and root morphology of Tiliaamurensis seedlings [J/OL]. Journal of Nanjing Forestry University(Natural Sciences Edition): 1-8[2019-06-30]. http://kns.cnki.net/kcms/detail/32.1161.S.20190319.1728.008.html.
[21]	王炎, 李振宙, 周良, 等. 不同施氮量对甜荞生长及产量的影响 [J]. 河南农业科学, 2018, 47(4):26−30. WANG Y, LI Z Z, ZHOU L, et al. Effect of different nitrogen application amount on growth and yield of common buckwheat [J]. Journal of Henan Agricultural Sciences, 2018, 47(4): 26−30.(in Chinese)
[22]	王益明, 万福绪, 胡菲, 等. 指数施肥对美国山核桃幼苗根系形态的影响 [J]. 东北林业大学学报, 2018, 46(3):29−32. doi: 10.3969/j.issn.1000-5382.2018.03.006 WANG Y M, WAN F X, HU F, et al. Effects of exponential fertilization on root morphology of pecan seedlings [J]. Journal of Northeast Forestry University, 2018, 46(3): 29−32.(in Chinese) doi: 10.3969/j.issn.1000-5382.2018.03.006
[23]	张会绒. 不同施氮水平对小麦幼苗性状的影响 [J]. 陇东学院学报, 2010, 21(5):77−80. doi: 10.3969/j.issn.1674-1730.2010.05.026 ZHANG H R. On the influence of executes N level differently on wheat seeding character [J]. Journal of Longdong University, 2010, 21(5): 77−80.(in Chinese) doi: 10.3969/j.issn.1674-1730.2010.05.026
[24]	甘娜, 陈其兵. 施氮对天彭牡丹(红丹兰、丹景红)形态特征的影响 [J]. 广东农业科学, 2012, 39(23):53−56. doi: 10.3969/j.issn.1004-874X.2012.23.017 GAN N, CHEN Q B. Effect of different nitrogen treatment on the morphology growth of Paeonia suffruticosa ‘Hongdan Lan’ and Paeonia suffruticosa ‘Danjing Hong’ [J]. Guangdong Agricultural Sciences, 2012, 39(23): 53−56.(in Chinese) doi: 10.3969/j.issn.1004-874X.2012.23.017
[25]	娄善伟, 张巨松, 罗新宁, 等. 施氮水平对棉花农艺性状、倒四叶光合特性及产量的影响 [J]. 新疆农业大学学报, 2009, 32(1):39−42. doi: 10.3969/j.issn.1007-8614.2009.01.009 LOU S W, ZHANG J S, LUO X N, et al. Influence of nitrogenous fertilizer level on agronomic characters, fourth leaf from top photosynthetic characteristics and yield of cotton [J]. Journal of Xinjiang Agricultural University, 2009, 32(1): 39−42.(in Chinese) doi: 10.3969/j.issn.1007-8614.2009.01.009
[26]	查琳, 余有祥, 袁紫倩, 等. 不同氮水平对盆栽‘奥斯特’北美冬青生长及座果的影响 [J]. 浙江林业科技, 2016, 36(1):51−54. doi: 10.3969/j.issn.1001-3776.2016.01.011 ZHA L, YU Y X, YUAN Z Q, et al. Effect of different nitrogen amount on growth and fruit setting of potted Ilex verticillata ‘oosterwijk’ [J]. Journal of Zhejiang Forestry Science and Technology, 2016, 36(1): 51−54.(in Chinese) doi: 10.3969/j.issn.1001-3776.2016.01.011
[27]	蒋士东, 周冀衡, 李强, 等. 不同处理对烟叶经济性状及化学协调性的影响 [J]. 中国农业科技导报, 2017, 19(11):100−107. JIANG S D, ZHOU J H, LI Q, et al. Effects of different treatments on economic characters and chemical coordination of flue cured tobacco [J]. Journal of Agricultural Science and Technology, 2017, 19(11): 100−107.(in Chinese)
[28]	郑小雨, 典瑞丽, 张建鹿, 等. 不同施氮水平对烤烟叶片农艺性状的影响 [J]. 湖南文理学院学报(自然科学版), 2019, 31(2):55−59. ZHENG X Y, DIAN R L, ZHANG J L, et al. Effects of different nitrogen levels on agronomic traits of flue-cured tobacco leaves [J]. Journal of Hunan University of Arts and Science(Science and Technology), 2019, 31(2): 55−59.(in Chinese)
[29]	姚一华, 蒋强, 董蒙蒙, 等. 不同施氮水平对块根膨大期木薯叶片光合特性的影响 [J]. 西南农业学报, 2017, 30(7):1516−1521. YAO Y H, JIANG Q, DONG M M, et al. Effect of nitrogen level on photosynthetic characteristics at stage of root Tuber expansion of cassava [J]. Southwest China Journal of Agricultural Sciences, 2017, 30(7): 1516−1521.(in Chinese)
[30]	曹高燚, 杜锦, 苏东伟, 等. 氮肥对春玉米‘中单509’叶片生理特性及产量的影响 [J]. 天津农学院学报, 2017, 24(2):1−4. doi: 10.3969/j.issn.1008-5394.2017.02.002 CAO G Y, DU J, SU D W, et al. Effects of nitrogen fertilizer on physiological characteristics of leaves and yield in spring maize ‘Zhongdan509’ [J]. Journal of Tianjin Agricultural University, 2017, 24(2): 1−4.(in Chinese) doi: 10.3969/j.issn.1008-5394.2017.02.002
[31]	张梅, 宋柏权, 杨骥, 等. 氮素对甜菜碳代谢产物的影响 [J]. 中国农学通报, 2016, 32(3):66−70. doi: 10.11924/j.issn.1000-6850.casb15100107 ZHANG M, SONG B Q, YANG J, et al. Effect of nitrogen on carbon metabolism matter of sugar beet [J]. Chinese Agricultural Science Bulletin, 2016, 32(3): 66−70.(in Chinese) doi: 10.11924/j.issn.1000-6850.casb15100107