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野生建兰根际与根内共生细菌种群结构差异分析

谢泰祥 张清华 周杰 陈娟 马山虎 艾叶

谢泰祥,张清华,周杰,等. 野生建兰根际与根内共生细菌种群结构差异分析 [J]. 福建农业学报,2020,35(5):560−568 doi: 10.19303/j.issn.1008-0384.2020.05.014
引用本文: 谢泰祥,张清华,周杰,等. 野生建兰根际与根内共生细菌种群结构差异分析 [J]. 福建农业学报,2020,35(5):560−568 doi: 10.19303/j.issn.1008-0384.2020.05.014
XIE T X, ZHANG Q H, ZHOU J, et al. Microbial Communities in Rhizosphere and Root-Endosphere of Wild Cymbidium ensifolium [J]. Fujian Journal of Agricultural Sciences,2020,35(5):560−568 doi: 10.19303/j.issn.1008-0384.2020.05.014
Citation: XIE T X, ZHANG Q H, ZHOU J, et al. Microbial Communities in Rhizosphere and Root-Endosphere of Wild Cymbidium ensifolium [J]. Fujian Journal of Agricultural Sciences,2020,35(5):560−568 doi: 10.19303/j.issn.1008-0384.2020.05.014

野生建兰根际与根内共生细菌种群结构差异分析

doi: 10.19303/j.issn.1008-0384.2020.05.014
基金项目: 福建省自然科学基金项目(2016J01703);国家自然科学基金项目(31700618);福建省种业创新与产业化工程项目(ZYCX-LY-2017005)
详细信息
    作者简介:

    谢泰祥(1995−),男,硕士研究生,主要从事园林植物栽培养护研究(E-mail:626060364@qq.com

    通讯作者:

    艾叶(1987−),女,讲师,博士,主要从事园林植物栽培育种方面研究(E-mail:360821887@qq.com

  • 中图分类号: Q 93;S 682.31

Microbial Communities in Rhizosphere and Root-Endosphere of Wild Cymbidium ensifolium

  • 摘要:   目的  比较野生建兰根际和根内细菌的种群结构差异,为研究根共生细菌与建兰的营养关系奠定理论基础。  方法  采用高通量测序技术鉴定福州鼓山野生建兰根际和根内共生细菌,分析其种类多样性和种群结构。  结果  建兰根际、根内拥有种类多样的细菌,辛普森指数分别为0.99、0.95;香农指数比较显示建兰根际细菌种类多样性显著高于根内。建兰根际拥有10门细菌,优势门为变形菌门(60.5%)、酸杆菌门(20.5%)、放线菌门(15.3%),根内优势细菌门分别为变形菌门(75.3%)、酸杆菌门(7.3%)、放线菌门(14.6%)。根际与根内丰度最高的10个细菌属中,SolibacterAcidipila仅在根际中,戴氏菌、新鞘氨醇菌和Granulicella仅存在于根内。根际中酸杆菌门酸杆菌纲的丰度显著高于根内,11个差异显著的目,仅肠杆菌目在根内的丰度大于根际,根际有12个属的丰度显著高于根内。  结论  建兰根际与根内细菌种类丰富度存在显著性差异,其原因可能是不同生态位的细菌对建兰的生物学功能存在较大差异。
  • 图  1  建兰根际与根内细菌16S rDNA高通量测序结果稀释曲线

    Figure  1.  Rarefaction curve of 16S rDNA high-throughput sequencing of bacteria in C. ensifolium rhizosphere soil and C. ensifolium root-endosphere

    图  2  建兰根际与根内细菌优势类群

    注:图中细菌无中文名称用黑线代替(图3~6同)。

    Figure  2.  Dominant groups of bacteria in rhizosphere soil and C. ensifolium roots

    Note: Bacteria absent Chinese names are shown by black lines (same for Figs. 3~6).

    图  3  建兰根际与根内细菌丰度最大的10个目

    Figure  3.  Dominant bacterial orders in in rhizosphere soil and C. ensifolium roots

    图  4  建兰根际与根内细菌丰度最大的10个属

    Figure  4.  Dominant bacterial genera in rhizosphere soil and C. ensifolium roots

    图  5  建兰根际与根内细菌不同分类单元下差异显著的类群

    Figure  5.  Significantly different bacterial groups of different taxa unit in rhizosphere soil and C. ensifolium roots

    图  6  建兰根际、根内共有的细菌OUT数量

    Figure  6.  Number of OUTs on bacteria commonly found in rhizosphere soil and C. ensifolium roots

    表  1  建兰根际土壤及根内部样品细菌OUTs丰度和α多样性指数

    Table  1.   OUTs richness and alpha diversity indices of bacteria in rhizosphere soil and C. ensifolium root-endosphere

    样品
    Sample
    有效序列
    Clean reads
    可操作分类单位
    OTUs
    α多样性指数 alphy diversity indices
    香农指数
    Shannon
    辛普森指数
    Simpson
    超1指数
    Chao1
    测序深度
    Good’s coverage
    根际 Rhiz 74 878±6 490 817±42 7.56±0.09* 0.99±0.00 865.43±36.11* 0.998±0.00
    根内 Endo 68 600±10 198 654±78 6.10±1.05* 0.95±0.04 700.99±67.30* 0.998±0.00
    注:*在P=0.05水平下,该指标根际与根内细菌存在显著性差异。
    Note: *at the level of P=0.05, there is a significant difference between this indicator rhizosphere and endosphere bacteria.
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  • 收稿日期:  2019-12-24
  • 修回日期:  2020-03-08
  • 刊出日期:  2020-05-01

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