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长期施用沼液对槟榔芋根际土壤真菌群落多样性的影响

刘兰英 黄薇 吕新 何肖云 陈丽华 李玥仁

刘兰英,黄薇,吕新,等. 长期施用沼液对槟榔芋根际土壤真菌群落多样性的影响 [J]. 福建农业学报,2020,35(9):1034−1042 doi: 10.19303/j.issn.1008-0384.2020.09.015
引用本文: 刘兰英,黄薇,吕新,等. 长期施用沼液对槟榔芋根际土壤真菌群落多样性的影响 [J]. 福建农业学报,2020,35(9):1034−1042 doi: 10.19303/j.issn.1008-0384.2020.09.015
LIU L Y, HUANG W, LYU X, et al. Effect of Long-term Biogas Slurry Application on Fungal Community in Rhizosphere Soil at Areca Taro Fields [J]. Fujian Journal of Agricultural Sciences,2020,35(9):1034−1042 doi: 10.19303/j.issn.1008-0384.2020.09.015
Citation: LIU L Y, HUANG W, LYU X, et al. Effect of Long-term Biogas Slurry Application on Fungal Community in Rhizosphere Soil at Areca Taro Fields [J]. Fujian Journal of Agricultural Sciences,2020,35(9):1034−1042 doi: 10.19303/j.issn.1008-0384.2020.09.015

长期施用沼液对槟榔芋根际土壤真菌群落多样性的影响

doi: 10.19303/j.issn.1008-0384.2020.09.015
基金项目: 福建省自然科学基金项目(2018J01037);福建省财政专项-福建省农业科学院科技创新团队建设项目(STIT2017-1-12)
详细信息
    作者简介:

    刘兰英(1987−),女,硕士,助理研究员,研究方向:农产品产地环境与过程控制(E-mail:lly87119@126.com

    通讯作者:

    李玥仁(1966−),男,博士,研究员,研究方向:农产品质量安全(E-mail:yuerenli@yeah.net

  • 中图分类号: X 172

Effect of Long-term Biogas Slurry Application on Fungal Community in Rhizosphere Soil at Areca Taro Fields

  • 摘要:   目的  探究施用沼液对槟榔芋根际土壤真菌群落多样性的影响,为沼液更好的还田利用提供理论依据。  方法  采集连续施用沼液6年和未施用沼液的槟榔芋种植地块土壤,通过Illumina Miseq高通量测序平台,分析土壤真菌群落多样性、组成及其与土壤理化性质间的关系,揭示影响真菌群落的主要土壤因子。  结果  与未施用沼液土壤比较,连续施用沼液6年的土壤有机质、碱解氮和速效钾含量都有明显提高。连续施用沼液后土壤真菌物种OTUs数量、群落丰富度指数和多样性指数也明显增加。真菌门水平鉴定出子囊菌门(Ascomycota)、担子菌门(Basidiomycota)、被孢霉门(Mortierellomycota)、球囊菌门(Glomeromycota)等类群,其中子囊菌门为土壤中丰富度最高的真菌类群。连续施用沼液明显增加了被孢霉门中被孢霉纲的相对丰度,而球囊菌门中球囊菌纲则大幅减少;连续施用沼液后土壤真菌属水平优势类群也发生明显改变。冗余分析结果表明,引起土壤真菌群落多样性变化最主要的环境因子为碱解氮、速效钾和有机质。  结论  长期施用沼液改变了槟榔芋根际土壤真菌所处的微环境,进而导致其群落组成和多样性发生变化。
  • 图  1  不同施肥方式土壤真菌OTU分布韦恩图

    注:CK,未施用沼液土壤;BS,沼液施肥6年土壤,下同。 

    Figure  1.  Venn graph on fungi OTU distribution in soil under varied fertilizations

    Note: CK, Soil with no biogas fertilization; BS, Soil with 6-year biogas slurry fertilization. Same for the following.

    图  2  不同施肥方式土壤真菌群落在门水平的相对丰度

    Figure  2.  Relative abundance of fungal community at phylum level in soil under varied fertilizations

    图  3  不同施肥方式土壤真菌群落在纲水平的相对丰度

    Figure  3.  Relative abundance of fungal community at class level in soil under varied fertilizations

    图  4  不同施肥方式土壤真菌群落在属水平聚类热图

    Figure  4.  Cluster heatmap of fungal community at genus level in soil under varied fertilizations

    图  5  不同施肥方式土壤真菌冗余分析

    注:OM,有机质;AN,碱解氮;AP,速效磷;AK,速效钾。

    Figure  5.  Redundancy analysis on fungi in soil under varied fertilizations

    Note: OM, Organic matter; AN, Alkali-hydrolysis N; AP, Available P; AK, Available K.

    表  1  不同施肥方式土壤理化性质

    Table  1.   Physicochemical characteristics of soil under varied fertilizations

    样品编号
    Samples ID
    pH有机质
    Organic matter/(g·kg−1
    碱解氮
    Alkali-hydrolysis N/(mg·kg−1
    速效磷
    Available P/(mg·kg−1
    速效钾
    Available K/(mg·kg−1
    CK5.29±0.01a 38.02±0.06b 229±0.28b 31.59±0.25a 190±0.76b
    BS5.27±0.0a39.61±0.01a254±2.47 a30.17±0.10a283±2.09a
    注:CK,未施用沼液土壤;BS,沼液施肥6年土壤;同列数据后不同小写字母表示处理间在0.05水平差异显著,表2同。
    Note: CK, Soil with no biogas fertilization; BS, Soil with 6-year biogas slurry fertilization; Data with different lowercase letters on same column indicate significant difference among treatments at 0.05 level. Same for Table 2.
    下载: 导出CSV

    表  2  不同施肥方式土壤真菌多样性分析

    Table  2.   Fungal diversity in soil under varied fertilizations

    样品编号 Samples IDChao1Observed speciesPD whole treeShannon
    CK 1 623±14.72b 1 252±18.88b 248±2.14b 6.72±0.26a
    BS2 116±57.86a1 582±66.11a305±6.34a6.87±0.23a
    下载: 导出CSV
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