Transient Expression of Gus Genes Controlled by Differential Regulator Sequences in Citrus Protoplasts
-
摘要: 通过PEG法及转化脂介导法将不同调控序列控制下的gus基因导入柑桔原生质体中,研究了gus基因在柑桔原生质体中的瞬间表达情况,结果表明,在柑桔原生质体中,控制gus基因表达水平的能力由大至小依次为CaMV35S启动子、AdhI启动子及rbcs启动子,内含子的存在可以提高gus基因在柑桔原生质体中的表达水平。Abstract: Gus genes controlled by several kinds of regulator sequences were introduced into citrus protoplasts respectively by PEG and lipofectin mediated transformation.The transient expression of gus gene in citrus protoplasts had been investigated.The expression level of the citrus protoplasts transformated with plasimid pBI121 and p35SGSINT (both gus genes were controlled by CaMV35S promoter) was higher than that transformated with plasmid pAT13 (gus gene controlled by Adhl promoter) and pRGN73 (gus gene under control of rice rbcs promoter).It was found that intron increase the expression level in citrus protoplasts.
-
Key words:
- Transient expression /
- Gus gene /
- Regulator sequenece /
- Citrus /
- Protoplasts
-
[2] 朱祯, 李向辉.植物遗传工程研究进展.生物工程进展, 1991, 11(1):35～40 [5] Choud hary A D, Kess mann H, et al.Stress responses in electroporated suspension cell prot oplasts.Plant Cell Rep., 1990, 9:42-46 [6] Hobbs S L A, J ackson J A, et al.Genetype-and promoter-ind uced variability intransient β-glucur on idase expression inpea protoplasts.Plant Cell Rep., 1990, 9:17-20 [7] Fromm M F, Taylor L P, et al.Expression of genest ransfered intomonocot and dicot plant cells by electroporation Proc.N et al.Acad.Sci.USA, 1985, 82:5842-5848 [9] Jefferson R A, K avanag h T A, et al.Gusfusions:β-glucu ronidaseas a senstive and versatile genefusion marker in higher plants.EMBO J., 1987, 6:3901-3907 [14] Cocking E C, Peberdy J F,(eds.).The use of protoplasts from fungi and higher plants as genetic systems.Dept.Bot any, Univ.Notting ham.1974 -
点击查看大图
计量
- 文章访问数: 2459
- HTML全文浏览量: 122
- PDF下载量: 837
- 被引次数: 0
下载:
