Abstract: In vitro screening of seed-derived callus lines of Asparagus was carried out by the biotechnology of plant cell culture,with the crude toxin filtrate extracted from mycelia of P.asparagi Sacc as stress factor.Some somatic mutants resistant to Asparagus stem blight disease were obtained from 638 calli screened, 62 calli were proliferative,from which 52 regenerated plants were produced.Disease resistance of the regenerated plants to P.asporagi Sacc was examined by spraying inoculation technique, out of 52 regenerated plants, 6 plants were resistant, and 46 plants were susceptible SE of all calli was 9.4×10-3 、Some problems about the utilization of resistant plants were also discussed.