Sensitivities of Rhizoctonia solani to Propiconazole and Pyraclostrobin on Rice Plants in Guizhou Province
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摘要:
目的 明确贵州省水稻纹枯病菌对丙环唑和吡唑醚菌酯的敏感性,为水稻纹枯病的科学防治及合理用药提供参考。 方法 采用菌丝生长速率法测定从贵州省4个地区采集分离的52株水稻纹枯病菌对丙环唑和吡唑醚菌酯的敏感性及药剂间的相关性。 结果 供试菌株对丙环唑的EC50值为0.058~2.381 μg·mL−1,均值为0.422 μg·mL−1;供试菌株对吡唑醚菌酯的EC50值范围从0.077~0.936 μg·mL−1,均值为0.313 μg·mL−1。抗性水平分析表明,湄潭地区的水稻纹枯病菌对丙环唑和吡唑醚菌酯出现了低抗水平的菌株,抗性频率分别为16.7%、13.3%。聚类分析表明,水稻纹枯病菌对丙环唑和吡唑醚菌酯的敏感性差异与菌株地理来源无明显相关性,且这两种药剂的敏感性之间也无显著相关性。 结论 供试菌株中多数菌株对丙环唑和吡唑醚菌酯的敏感性较高。药剂间未产生交互抗性,生产上可以交替使用。 Abstract:Objective Sensitivities of Rhizoctonia solani on rice to propiconazole and pyraclostrobin were determined for proper pesticide application on the disease control in Guizhou. Method Mycelium growth rate was used as the criterion for testing the pesticide sensitivities of 52 strains of R. solani collected from 4 regions in the province. Result The EC50 of propiconazole on the pathogens ranged from 0.058 μg·mL−1 to 2.381 μg·mL−1, averaging 0.422 μg·mL−1. That of pyraclostrobin were between 0.077 μg·mL−1 and 0.936 μg·mL−1, averaging 0.313 μg·mL−1. Strains of low resistance to the pesticides were found in Meitan area of the province with a resistance frequency of 16.7% on propiconazole and 13.3% on pyraclostrobin. A cluster analysis showed no significant correlation between the pesticide sensitivities and the geographical origins of the pathogens, nor between the two pesticides. Conclusion A large number of the R. solani isolates in the study were highly sensitive to propiconazole and pyraclostrobin, and no apparent cross-resistance observed between the two fungicides. Hence, alternative application of them for disease control was deemed appropriate. -
Key words:
- Rhizoctonia solani /
- propiconazole /
- pyraclostrobin /
- pesticide sensitivity /
- cross-resistance
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表 1 不同地区纹枯病菌对丙环唑和吡唑醚菌酯的敏感性
Table 1. Pesticide sensitivities of R. solani from different regions
采集地
Locality菌株数
Isolate number丙环唑 propiconazole 吡唑醚菌酯 pyraclostrobin EC50范围
Range of EC50/(μg·mL−1)EC50均值
Average value of EC50/(μg·mL−1)EC50范围
Range of EC50/(μg·mL−1)EC50均值
Average value of EC50/(μg·mL−1)惠水 HS Huishui 13 0.065~0.519 (0.256±0.048)b 0.077~0.234 (0.153±0.016)b 湄潭 MT Meitang 18 0.077~2.381 (0.623±0.169)a 0.156~0.936 (0.525±0.062)a 黄平 HP Huangping 12 0.066~0.209 (0.140±0.013)b 0.126~0.383 (0.241±0.020)b 三穗 SS Sansui 9 0.498~1.060 (0.635±0.056)a 0.127~0.317 (0.217±0.019)b 注:同列数字后不同字母表示经新复极差法检验差异显著(P<0.05)。
Note: Data followed by the different letters are significantly by new multiple range test( P <0.05).表 2 不同地区水稻纹枯病菌对丙环唑和吡唑醚菌酯的抗性水平
Table 2. Pesticide resistances of R. solani from different regions
采集地
Locality菌株数
Isolate number丙环唑 吡唑醚菌酯 不同抗性水平菌株频率
Frequencies at different resistance level/%抗性频率
Resistance frequencies/%抗性水平范围
Range of resistance level不同抗性水平菌株频率
Frequencies at different resistance level/%抗性频率
Resistance frequencies/%抗性水平范围
Range of resistance levelRf<3 3<Rf≤10 Rf<3 3<Rf≤10 惠水
HS Huishui13 100 0 0 0.14~1.23 100 0 0 0.24~0.75 湄潭
MT Meitang18 83.3 16.7 16.7 0.18~5.64 86.6 13.3 13.3 0.50~3.01 黄平
HP Huangping12 100 0 0 0.16~0.50 100 0 0 0.40~1.22 三穗
SS Sansui9 100 0 0 1.18~2.51 100 0 0 0.41~1.01 -
[1] CHEN Z X, FENG Z M, KANG H X, et al. Identification of new resistance loci against sheath blight disease in rice through genome-wide association study [J]. Rice Science, 2019, 26(1): 21−31. doi: 10.1016/j.rsci.2018.12.002 [2] FENG S J, SHU C W, WANG C, et al. Survival of Rhizoctonia solani AG-1 IA, the causal agent of rice sheath blight, under different environmental conditions [J]. Journal of Phytopathology, 2017, 165(1): 44−52. doi: 10.1111/jph.12535 [3] 谭清群, 何海永, 陈小均, 等. 贵州水稻纹枯病菌对噻呋酰胺和己唑醇的敏感性测定 [J]. 四川农业大学学报, 2017, 35(2):159−166.TAN Q Q, HE H Y, CHEN X J, et al. Study on sensitivity of Rhizoctonia solani of rice to thifluzamide and texaconazole in Guizhou Province [J]. Journal of Sichuan Agricultural University, 2017, 35(2): 159−166.(in Chinese) [4] LIU L M, LIANG M Q, LI L, et al. Synergistic effects of the combined application of Bacillus subtilis, H158 and strobilurins for rice sheath blight control [J]. Biological Control, 2018, 117: 182−187. doi: 10.1016/j.biocontrol.2017.11.011 [5] 吴婕, 席亚东, 李洪浩, 等. 四川省水稻纹枯病菌对井冈霉素抗药性监测 [J]. 西南农业学报, 2015, 28(6):2501−2504.WU J, XI Y D, LI H H, et al. Monitoring of resistance of Thenatephorus cucumeris to jinggangmysin in Sichuan [J]. Southwest China Journal of Agricultural Sciences, 2015, 28(6): 2501−2504.(in Chinese) [6] 陈小龙, 方夏, 沈寅初. 纹枯病菌对井冈霉素的作用机制、抗药性及安全性 [J]. 农药, 2010, 49(7):481−483. doi: 10.3969/j.issn.1006-0413.2010.07.004CHEN X L, FANG X, SHEN Y C. Mechanism, resistance and security of Jinggangmycin against Rhizoctonia solani [J]. Agrochemicals, 2010, 49(7): 481−483.(in Chinese) doi: 10.3969/j.issn.1006-0413.2010.07.004 [7] 连娜娜, 纪明山, 祁之秋, 等. 东北三省水稻纹枯病病菌对苯醚甲环唑和咪鲜胺的敏感性 [J]. 农药, 2018, 57(9):699−702.LIAN N N, JI M S, QI Z Q, et al. Sensitive baseline of Rhizoctonia solani to Difenoconazole and Prochloraz in northeast three province [J]. Agrochemicals, 2018, 57(9): 699−702.(in Chinese) [8] 唐小飞, 张夕林. 吡唑醚菌酯防治水稻纹枯病、穗颈瘟的效果及应用技术 [J]. 上海农业科技, 2016(6):128−129. doi: 10.3969/j.issn.1001-0106.2016.06.072TANG X F, ZHANG X L. Effect and application technology of pyraclostrobin on Rice Sheath Blight and Rice blast [J]. Shanghai Agricultural Science and Technology, 2016(6): 128−129.(in Chinese) doi: 10.3969/j.issn.1001-0106.2016.06.072 [9] 张景飞, 袁月芳, 须兆龙, 等. 不同药剂对水稻纹枯病、稻曲病的药效试验 [J]. 上海农业科技, 2013(4):126, 122.ZHANG J F, YUAN Y F, XU Z L, et al. Effects of Different Pesticides on Rice Sheath Blight and Rice False Smut [J]. Shanghai Agricultural Science and Technology, 2013(4): 126, 122.(in Chinese) [10] 汪汉成, 周明国, 张艳军, 等. 戊唑醇对立枯丝核菌的抑制作用及在水稻上的应用 [J]. 农药学学报, 2007, 9(4):357−362. doi: 10.3321/j.issn:1008-7303.2007.04.008WANG H C, ZHOU M G, ZHANG Y J, et al. Fungicidal Activity of Tebuconazole against Rhizoctonia solani and its Application to Rice [J]. Chinese Journal of Pesticide Science, 2007, 9(4): 357−362.(in Chinese) doi: 10.3321/j.issn:1008-7303.2007.04.008 [11] 唐正合, 汪汉成, 王建新, 等. 丙环唑对水稻纹枯病菌的抑制作用及对纹枯病的防治效果 [J]. 植物保护, 2012, 38(1):158−161. doi: 10.3969/j.issn.0529-1542.2012.01.034TANG Z H, WANG H H, WANG J X, et al. Fungicidal activity of propiconazole to Rhizoctonia solani and its control efficacy against rice sheath blight [J]. Plant Protection, 2012, 38(1): 158−161.(in Chinese) doi: 10.3969/j.issn.0529-1542.2012.01.034 [12] 梁梦琦. 长江中下游稻区稻瘟病菌对稻瘟灵和吡唑醚菌酯的抗性监测[D]. 北京: 中国农业科学院, 2018.LIANG M Q. Monitoring sensitivity of Magnaporthe oryzae to Isoprothiolane and pyraclostrobin in middle and lower reaches of Yangtze[D]. Beijing: Chinese Academy of Agricultural Sciences, 2018. (in Chinese) [13] 孙雪, 侯志广, 赵晓峰, 等. 多种药剂对水稻纹枯病菌的毒力测定及田间药效 [J]. 农药, 2015, 54(2):139−142.SUN X, HOU Z G, ZHAO X F, et al. Toxicity assay and field control effects of different fungicides against Rhizoctonia solani [J]. Agrochemicals, 2015, 54(2): 139−142.(in Chinese) [14] 刘郁, 于亚辉. 吡唑醚菌酯施用时期对水稻主要真菌病害防效的影响 [J]. 福建农业学报, 2016, 31(2):175−178.LIU Y, YU Y H. Effect of Pyraclostrobin Application time on controlling major fungal diseases on rice in coastal regions of Liaoning [J]. Fujian Journal of Agricultural Sciences, 2016, 31(2): 175−178.(in Chinese) [15] 周而勋, 杨媚. 从植物病组织中分离立枯丝核菌的快速、简便技术 [J]. 华南农业大学学报, 1998, 19(1):125−126.ZHOU E X, YANG M. A Rapid and simple technique for the isolation of Rhizoctonia solani from diseased plant tissues [J]. Journal of South China Agricultural University, 1998, 19(1): 125−126.(in Chinese) [16] 胡贤锋, 李荣玉, 王健, 等. 水稻稻曲病菌的分离鉴定及室内毒力测定 [J]. 福建农业学报, 2017, 32(6):634−638.HU X F, LI R Y, WANG J, et al. Isolation, identification and fungicide toxicity of Ustilaginoidea virens [J]. Fujian Journal of Agricultural Sciences, 2017, 32(6): 634−638.(in Chinese) [17] 徐建强, 刁兴旺, 李恒, 等. 中国河南省小麦纹枯病菌对苯醚甲环唑及戊唑醇的敏感性 [J]. 农药学学报, 2016, 18(5):582−588.XU J Q, DIAO X W, LI H, et al. Sensitivity to difenoconazole and tebuconazole of Rhizoctonia cerealis in Henan province in China [J]. Chinese Journal of Pesticide Science, 2016, 18(5): 582−588.(in Chinese) [18] 杨帆, 李荣玉, 李明, 等. 水稻纹枯病病菌对苯醚甲环唑的敏感性基线 [J]. 中国植保导刊, 2017, 37(5):67−70. doi: 10.3969/j.issn.1672-6820.2017.05.013YANG F, LI R Y, LI M, et al. Sensitive baseline of Rhizoctonia solani to difenoconazole [J]. China Plant Protection, 2017, 37(5): 67−70.(in Chinese) doi: 10.3969/j.issn.1672-6820.2017.05.013 [19] 孙树青, 李宁波, 曾凯. 丙环唑对水稻纹枯病菌的抑制作用及对纹枯病的防治效果 [J]. 农业科技与信息, 2016(26):87−88. doi: 10.3969/j.issn.1003-6997.2016.26.058SUN S Q, LI N B, ZENG K. Fungicidal activity of propiconazole to Rhizoctonia solani and its control efficacy against rice sheath blight [J]. Information of Agricultural Science and Technology, 2016(26): 87−88.(in Chinese) doi: 10.3969/j.issn.1003-6997.2016.26.058 [20] 孙雪. 水稻纹枯病的防治技术研究[D]. 长春: 吉林农业大学, 2015.SUN X. Research on prevention and treatment technology of Rhizoctonia solani[D]. Changchun: Jilin Agricultural University, 2015. (in Chinese) [21] 范文艳, 文景芝, 金丽娜, 等. 黑龙江省水稻纹枯病菌的致病力分化与AFLP分析 [J]. 植物保护, 2008, 34(6):57−61. doi: 10.3969/j.issn.0529-1542.2008.06.013FAN W Y, WEN J Z, JIN L N, et al. Pathogenic differentiation and AFLP analysis of the rice sheath blight strains from Heilongjiang [J]. Plant Protection, 2008, 34(6): 57−61.(in Chinese) doi: 10.3969/j.issn.0529-1542.2008.06.013 [22] 陈涛, 张震, 柴荣耀, 等. 浙江省水稻纹枯病菌的遗传分化与致病力研究 [J]. 中国水稻科学, 2010, 24(1):67−72. doi: 10.3969/j.issn.1001-7216.2010.01.12CHEN T, ZHANG Z, CAI R Y, et al. Genetic diversity and pathogenicity variation of different Rhizoctonia solani isolates in rice from Zhejiang Province, China [J]. Chinese Journal of Rice Science, 2010, 24(1): 67−72.(in Chinese) doi: 10.3969/j.issn.1001-7216.2010.01.12 [23] 刘志恒, 韩翔宇, 杨红, 等. 辽宁省水稻纹枯病菌菌丝融合群鉴定初报 [J]. 沈阳农业大学学报, 2010, 41(1):82−85. doi: 10.3969/j.issn.1000-1700.2010.01.018LIU Z H, HAN X Y, YANG H, et al. Identification of Anastomosis Groups of Rhizoctonia solani on Rice in Liaoning Province [J]. Journal of Shenyang Agricultural University, 2010, 41(1): 82−85.(in Chinese) doi: 10.3969/j.issn.1000-1700.2010.01.018 [24] 张优, 魏松红, 王海宁, 等. 东北地区水稻纹枯病菌遗传多样性和致病性分析 [J]. 沈阳农业大学学报, 2017, 48(1):9−14.ZHANG Y, WEI S H, WANG H N, et al. Genetic diversity and pathogenicity of Rhizoctonia solani isolates causing sheath blight disease of rice in northeast region [J]. Journal of Shenyang Agricultural University, 2017, 48(1): 9−14.(in Chinese)