水稻T-DNA插入启动子捕获系的初步筛选

胡昌泉; 刘华清; 李素; 潘大仁; 王锋

水稻T-DNA插入启动子捕获系的初步筛选

胡昌泉^1,2,
刘华清²,
李素²,
潘大仁¹,
王锋^2, ,

1.
福建农林大学生命科学学院, 福建福州350002;
2.
福建省农业科学院生物技术研究所, 福建省农业遗传工程重点实验室, 福建福州350003

基金项目:

福建省科技计划——省属公益类科研院所基本科研专项(2009R10035-3);福建省自然科学基金项目(2010J01099)

详细信息

通讯作者:
王锋(1963-),男,博士,研究员,从事植物基因工程研究(E-mail:wf@fjage.org)

中图分类号: Q78
计量
- 文章访问数: 2541
- HTML全文浏览量: 74
- PDF下载量: 684
- 被引次数: 0
出版历程
- 收稿日期: 2011-01-12
- 修回日期: 2011-02-17
- 刊出日期: 2011-04-15

Preliminary Screening of Rice Promoter Trap Lines with T-DNA Tagging

1.
Life Sciences College, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350013, China;
2.
Key Laboratory of Agricultural Genetic Engineering, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China

摘要

摘要: 利用农杆菌介导法将含报道基因β-glucuronidase(GUS)无启动子的转化载体pCAMBIA1300GUSA-Hyg导入籼稻明恢86获得水稻启动子捕获系。水稻启动子捕获系潮酶素抗感分离比χ2分析表明:39个水稻启动子捕获系中有28个为单T-DNA位点插入。2653个水稻启动子捕获系报告基因GUS表达检测结果表明:83个水稻启动子捕获系在其不同时期、不同器官(包括根、茎、叶、花药、颖壳、胚、胚乳等组织)中检测到GUS表达。其中GUS活性在胚乳有表达的水稻启动子捕获系24个,占检测总数0.91%;GUS活性在花药中有表达的水稻启动子捕获系为19个,占检测总数0.72%。
- 水稻 /
- 启动子捕获系 /
- T-DNA标签
Abstract: Binary vector pCAMBIA1300GUSA-Hyg containing β-glucuronidase(GUS) without the promoter was introduced into Oryza sativa L.Minghui 86 by agrobacterium mediated transformation.The χ2 tests on the hygromycin resistant ratio revealed that 28 of the 39 rice promoter trap lines contained a loci T-DNA tagging.GUS histochemical assay at different stages in different organs,such as root,stem,leave,anther,hull,embryo and emdospern,of 2653 rice promoter trap lines was performed.The results showed that 83 rice promoter lines were GUS positive.Of all tested,24 lines were expressed in endosperm(i.e.,0.91%) and 19 lines expressed in anther(i.e.,0.72%).Information collected on the rice promoter trap lines could be useful in identifying new rice genes.
- Rice /
- promoter trap lines /
- T-DNA tagging

HTML全文

参考文献(4)

[1]	JEON JS,LEE S,JUNG KH,et al.T-DNA insertional mutagenesis for functional genomics in rice[J].Plant J,2000,22 (6):561-70.
[2]	SPRINGER PS.Gene traps:tool for plant development and genomics[J].Plant Cell,2000,12:1007-1020.
[3]	JEFFERSON R A.Assaying chimeric genes in plants:the GUS gene fusion system[J].Plant Molecular Biology Reporter,1987,5:387.
[4]	ZHANG J,LI C,WU C,et al.RMD:a rice mutant database for functional analysis of the rice genome[J].Nucleic Acids Res,2006,1 (34):745-748.