摘要:
研究结果表明,土壤和作物根部存在大量有益的拮抗细菌,其中A06、A209和S34,3个菌株对芦笋茎枯病有很强的拮抗作用,对病菌菌丝体生长的抑制宽度分别为10.6mm,10.0mm和8.6mm;对器孢子萌发的抑制圈半径分别为9.3mm,8.7mm和9.2mm,且这些菌株的发酵液也有抑菌作用。室内平板测定结果表明:适宜拮抗细菌生长温度为25～35℃,pH值6～8;距紫外线光源30～100cm内照射5min,拮抗细菌均被杀伤,抑制细菌生长率98.9%～31.5%.在田间,拮抗菌液和麦皮混合施用,防治茎枯病效果为44.6%。
Abstract:
There existed a large number of beneficial antagonistic bacteria against Phoma asparagi Sacc.in the root soil of asparagus crops,among which A06,A209,and S34,have been selected to inhibit P.asparagi.The girdle bands of antagonistic bacteria against the mycelium growth were 10.6,10.0,8.6mm respectively,that against the spore germination were 9.3,8.7,9.2 mm respectively.The fermentation liquid of the bacteria had the strong inhibition to the disease.The suitable conditions for the bacteria growing were 25-35℃ temperature with 6-8pH.The antagonistic bacteria were wounded and restrained under ultraviolet in the distance of 300-1000 mm in 5 minutes.The efficiency of controlling the disease using the product of the antagonistic bacteria in the field was about 44.6%.
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