枇杷组织中糖的气相色谱测定
Determination on Sugar Content in Loquat Constitution by Chromatography
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摘要: 本文首次报道枇杷种子中存在D-半乳糖并建立了枇杷组织中5种糖的简易测定方法。采用大口径毛细管柱对枇杷组织中存在的D-果糖、D-半乳糖、D-葡萄糖、D-山梨醇、蔗糖等5种糖进行定量分析。本方法对各组分的线性相关系数r均大于0.999;变异系数CV均小于3%;对山梨醇的平均回收率为98%.Abstract: This article first reported the existence of D-galactose in loquat seeds and the establishment of a brief determining method for five kinds of carbohydrate in loquat constitute, i.e.D-Fructose, D-Galactose, D-Glucose, D-Sorbitol and Sucrose.The quantitative analysis was carried out through a large-bore capillary column.This methed more than 0.999 the linear correlation coefficient for each analysed componence, less than 3% the coefficient of variation and the average recovery of D-Sorbitol reaches 98%.
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Key words:
- Gas Chromatography /
- Loquat /
- D-Fructose /
- D-Galactose /
- D-Glucose /
- D-Sorbitol /
- Sucrose
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[1] 田庚元.1985.天花粉多糖的研究I.分离、纯化及其理化性质.生物化学与生物物理学报,17(5):582~585 [3] 李明元主编.1988.高效液相色谱及其在食品分析中的应用.151~155.北京大学出版社,北京 -
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