摘要:
本文研究了褐飞虱种群酯酶同工酶酶谱型组成结构以及在不同生境下的变化规律。研究结果表明褐飞虱酯酶同工酶(EST)酶谱模式为三条带,即A、B、C,迁移率分别为0.4102、0.4709、0.5042。由三条酶带组成4种个体酶谱型:Ⅰ型C、Ⅱ型BC、Ⅲ型AC、Ⅳ型ABC。褐飞虱种群由这4种类型的个体按不同的比例组成,形成了种群的异质性。在稻田测报灯下,褐飞虱种群初建时期(5月份),Ⅰ型比例较低为0.46,Ⅱ型比例较高为0.31,随着种群的发展Ⅰ型比例稳定在0.6左右,Ⅱ型比例在0.2—0.36之间;11月份水稻生长季节结束时,Ⅰ型比例上升到0.9,Ⅱ型比例下降到0.098。测报灯下,田间基部,人工繁殖的褐飞虱种群EST酶谱比例也有很大差异,分析结果表明,Ⅰ型个体的比例高,指示着褐飞虱种群处于较健康状态。
Abstract:
The variation regularity of esterase (EST) zymogram in brown planthopper (Nilaparvata lugens Stal) population during rice-growing stages or at different habitats was studied.The results showed that there were three bands designating as A, B and C bands with transferring rates of 0.4102, 0.4709 and 0.5042 respectively, which composed four zymogram types, i.e.I -c, II -BC, III -AC and IV-ABC, in EST zymo-gram of brown planthopper population.In the rice-growing season, the brown planthopper population was consisted of different individuals possessing these four zymogram types, and formed the population heterogeneity.At the begining of population establishment (in May), the rate of type I (0.46) was lower, it increased with the population development and maintained stably around 0.6.In November, the terminal of rice-growing season, the rate of type I went up to 0.9, there were also significant differences of the EST zymogram in N.Lugens among the samples from black lig’lt trap, lower part of rice plant and artificial propagation under caged condition.The analysis results showed that higher proportion of type I indicates the more healthy situation of brown planthopper population.
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