甘薯品种的抗瘟性与酯酶、过氧化物酶同工酶的关系研究
Research about Relationship Between The Isozymes of Esterase Peroxidase and the Resistance to Pseudomonas solanacearum in Sweet Potato Varieties
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摘要: 在聚丙烯酰胺垂直平板凝胶电泳分析中,从不同抗瘟性甘薯品种中获得的酯酶同工酶谱由多种酶带以不同的比例构成。抗病、中抗和感病品种各有其特异的酶谱型。酶谱型6,4和5、7、10分别是抗病,中抗和感病品种的特异酶谱型。这一结果可以作为鉴定甘薯品种抗瘟性的生化指标之一。扫描分析结果:病、健薯苗过氧化物酶同工酶Pa区吸光值比的大小,也可以作为鉴定甘薯品种抗瘟性的生化指标之一。Abstract: In vertical polyacrylamide slab gel electrophoresis analysis,the band patterns of esterase isozymes obtained from resistance differing sweet potato varieties to Pseudomonas solanaccarum are composed of many enzyme bands in proper ratio.The high,medium or susceptoble resistance varieties had their own band patters of esterase isozynes.Pattern 6 represents high resistance,pattern 4 medium and pattern 5,7,10 susceptible.The band patterns of esterase isozymes can be used as one of biochemical criteria in identification of the variety resistance,Being analyzed by ultraiolet ray screening,the ratio of OD readings in Pa area of peroxidase isozymes in diseased or health seedlings of sweet potato can also be used as one of biochemical criteria in identification of the variety resistance.
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